455 results on '"Krmpotić A"'
Search Results
2. Beyond Artists’ Colors: A Spectral Reference Database for the Identification of β‑Naphthol and Triarylcarbonium Colorants by MeV SIMS
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Teodora Raicu, Matea Krmpotić, Zdravko Siketić, Iva Bogdanović Radović, Katja Sterflinger, and Dubravka Jembrih-Simbürger
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Chemistry ,QD1-999 - Published
- 2024
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3. A single-dose MCMV-based vaccine elicits long-lasting immune protection in mice against distinct SARS-CoV-2 variants
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Kristin Metzdorf, Henning Jacobsen, Yeonsu Kim, Luiz Gustavo Teixeira Alves, Upasana Kulkarni, Maja Cokarić Brdovčak, Jelena Materljan, Kathrin Eschke, M. Zeeshan Chaudhry, Markus Hoffmann, Federico Bertoglio, Maximilian Ruschig, Michael Hust, Marko Šustić, Astrid Krmpotić, Stipan Jonjić, Marek Widera, Sandra Ciesek, Stefan Pöhlmann, Markus Landthaler, and Luka Čičin-Šain
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SARS-CoV-2 ,COVID-19 ,MCMV ,vaccination ,single-dose ,long-lasting protection ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Current vaccines against COVID-19 elicit immune responses that are overall strong but wane rapidly. As a consequence, the necessary booster shots have contributed to vaccine fatigue. Hence, vaccines that would provide lasting protection against COVID-19 are needed, but are still unavailable. Cytomegaloviruses (CMVs) elicit lasting and uniquely strong immune responses. Used as vaccine vectors, they may be attractive tools that obviate the need for boosters. Therefore, we tested the murine CMV (MCMV) as a vaccine vector against COVID-19 in relevant preclinical models of immunization and challenge. We have previously developed a recombinant MCMV vaccine vector expressing the spike protein of the ancestral SARS-CoV-2 (MCMVS). In this study, we show that the MCMVS elicits a robust and lasting protection in young and aged mice. Notably, spike-specific humoral and cellular immunity was not only maintained but also even increased over a period of at least 6 months. During that time, antibody avidity continuously increased and expanded in breadth, resulting in neutralization of genetically distant variants, like Omicron BA.1. A single dose of MCMVS conferred rapid virus clearance upon challenge. Moreover, MCMVS vaccination controlled two variants of concern (VOCs), the Beta (B.1.135) and the Omicron (BA.1) variants. Thus, CMV vectors provide unique advantages over other vaccine technologies, eliciting broadly reactive and long-lasting immune responses against COVID-19.
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- 2024
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4. Pediatric pulmonary thromboembolism: a 3-year Canadian Pediatric Surveillance Program study
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Krmpotic, Kristina, Ramsay, Lily, McMullen, Sarah, Chan, Anthony K.C., Plint, Amy C., and Moorehead, Paul
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- 2024
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5. Pediatric Critical Care Referrals for Tertiary Inpatient and Transport Services in Canada's Maritime Provinces: A Retrospective Cohort Study
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Craig, Stephanie, Foster, Jennifer, Gallant, Julien, Verma, Neeraj, and Krmpotic, Kristina
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- 2024
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6. Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells
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Carmen Rožmanić, Berislav Lisnić, Marina Pribanić Matešić, Andrea Mihalić, Lea Hiršl, Eugene Park, Ana Lesac Brizić, Daniela Indenbirken, Ina Viduka, Marina Šantić, Barbara Adler, Wayne M. Yokoyama, Astrid Krmpotić, Vanda Juranić Lisnić, Stipan Jonjić, and Ilija Brizić
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Science - Abstract
Abstract Infections in early life can elicit substantially different immune responses and pathogenesis than infections in adulthood. Here, we investigate the consequences of murine cytomegalovirus infection in newborn mice on NK cells. We show that infection severely compromised NK cell maturation and functionality in newborns. This effect was not due to compromised virus control. Inflammatory responses to infection dysregulated the expression of major transcription factors governing NK cell fate, such as Eomes, resulting in impaired NK cell function. Most prominently, NK cells from perinatally infected mice have a diminished ability to produce IFN-γ due to the downregulation of long non-coding RNA Ifng-as1 expression. Moreover, the bone marrow’s capacity to efficiently generate new NK cells is reduced, explaining the prolonged negative effects of perinatal infection on NK cells. This study demonstrates that viral infections in early life can profoundly impact NK cell biology, including long-lasting impairment in NK cell functionality.
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- 2023
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7. A genetic history of the Balkans from Roman frontier to Slavic migrations
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Olalde, Iñigo, Carrión, Pablo, Mikić, Ilija, Rohland, Nadin, Mallick, Swapan, Lazaridis, Iosif, Mah, Matthew, Korać, Miomir, Golubović, Snežana, Petković, Sofija, Miladinović-Radmilović, Nataša, Vulović, Dragana, Alihodžić, Timka, Ash, Abigail, Baeta, Miriam, Bartík, Juraj, Bedić, Željka, Bilić, Maja, Bonsall, Clive, Bunčić, Maja, Bužanić, Domagoj, Carić, Mario, Čataj, Lea, Cvetko, Mirna, Drnić, Ivan, Dugonjić, Anita, Đukić, Ana, Đukić, Ksenija, Farkaš, Zdeněk, Jelínek, Pavol, Jovanovic, Marija, Kaić, Iva, Kalafatić, Hrvoje, Krmpotić, Marijana, Krznar, Siniša, Leleković, Tino, M. de Pancorbo, Marian, Matijević, Vinka, Milošević Zakić, Branka, Osterholtz, Anna J., Paige, Julianne M., Tresić Pavičić, Dinko, Premužić, Zrinka, Rajić Šikanjić, Petra, Rapan Papeša, Anita, Paraman, Lujana, Sanader, Mirjana, Radovanović, Ivana, Roksandic, Mirjana, Šefčáková, Alena, Stefanović, Sofia, Teschler-Nicola, Maria, Tončinić, Domagoj, Zagorc, Brina, Callan, Kim, Candilio, Francesca, Cheronet, Olivia, Fernandes, Daniel, Kearns, Aisling, Lawson, Ann Marie, Mandl, Kirsten, Wagner, Anna, Zalzala, Fatma, Zettl, Anna, Tomanović, Željko, Keckarević, Dušan, Novak, Mario, Harper, Kyle, McCormick, Michael, Pinhasi, Ron, Grbić, Miodrag, Lalueza-Fox, Carles, and Reich, David
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- 2023
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8. A Nuclear Structure Model for Double Charge-Exchange Processes
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Ferreira, V. dos S., Samana, A. R., Krmpotić, F., and Chiapparini, M.
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Nuclear Theory - Abstract
A new model, based on the BCS approach, is specially designed to describe nuclear phenomena $(A,Z)\rightarrow (A,Z\pm 2)$ of double-charge exchange (DCE). After being proposed, and applied in the particle-hole limit, by one of the authors (F. Krmpoti\'c [1]), so far it was never been applied within the BCS mean-field framework, nor has its ability to describe DCE processes been thoroughly explored. It is a natural extension of the pn-QRPA model, developed by Halbleib and Sorensen [2] to describe the single $\beta$-decays $(A,Z)\rightarrow (A,Z\pm 1)$, to the DCE processes. As such, it exhibits several advantages over the pn-QRPA model when is used in the evaluation of the double beta decay (DBD) rates. For instance, i) the extreme sensitivity of the nuclear matrix elements (NMEs) on the model parametrization does not occur, ii) it allows to study NMEs, not only for the fundamental state in daughter nuclei, as the pn-QRPA model does, but also for all final $0^+$ and $2^+$ states, accounting at the same time their excitation energies and the corresponding DBD Q-values, iii) together with the DBD-NMEs it provides also the energy spectra of Fermi and Gamow-Teller DCE transition strengths, as well as the locations of the corresponding resonances and their sum rules, iv) the latter are relevant for both the DBD and the DCE reactions, since the involved nuclear structure is the same; this correlation does not exist within the pn-QRPA model. As an example, detailed numerical calculations are presented for the $(A,Z)\rightarrow (A,Z+ 2)$ process in $^{48}$Ca $\rightarrow ^{48}$Ti and the $(A,Z)\rightarrow (A,Z- 2)$ process in $^{96}$Ru $\rightarrow ^{96}$Mo, involving all final $0^+$ states and $2^+$ states., Comment: 17 pages, 9 figures. To be submitted to Phys. Rev. C
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- 2019
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9. Nonmesonic weak decay of charmed hypernuclei
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Fontoura, C. E., Krmpotić, F., Galeão, A. P., De Conti, C, and Krein, G.
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Nuclear Theory ,High Energy Physics - Lattice ,Nuclear Experiment - Abstract
We present a study of the nonmesonic weak decay (NMWD) of charmed hypernuclei using a relativistic formalism. We work within the framework of the independent particle shell model and employ a ($\pi$,$K$) one-meson-exchange model for the decay dynamics. We implement a fully relativistic treatment of nuclear recoil. Numerical results are obtained for the one-neutron-induced transition NMWD rates of the $_{\Lambda^{+}_{c}}^{12}$N. The effect of nuclear recoil is sizable and goes in the direction to decrease the nuclear decay rate. We found that the NMWD decay rate of $_{\Lambda^{+}_{c}}^{12}$N is of the same order of magnitude as the partial decay rate for the corresponding mesonic decay $\Lambda^+_c \rightarrow \Lambda + \pi^+$, suggesting the feasibility of experimental detection of such heavy-flavor nuclear processes., Comment: 24 pages, 3 figures
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- 2017
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10. Partial restoration of spin-isospin SU(4) Symmetry and the One-QRPA method in Double Beta Decay
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Ferreira, V. dos S., Krmpotić, F., Barbero, C. A., and Samana, A. R.
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Nuclear Theory - Abstract
The one-QRPA method is used to describe simultaneously both double decay beta modes, giving special attention to the partial restoration of spin-isospin SU(4) symmetry. To implement this restoration and to fix the model parameters, we resort to the energetics of Gamow-Teller resonances and to the minima of the single $\beta^+$-decay strengths. This makes the theory predictive regarding the $\beta\beta_{2\nu}$-decay, producing the $2\nu$ moments in $^{48}$Ca, $^{76}$Ge, $^{82}$Se, $^{96}$Zr, $^{100}$Mo, $^{128,130}$Te, and $^{150}$Nd, that are of the same order of magnitude as the experimental ones; however, the agreement with $\beta\beta_{2\nu}$ data is only modest. To include contributions coming from induced nuclear weak currents, we extend the $\beta\beta_{0\nu}$-decay formalism employed previously in C. Barbero et. al, Nuc. Phys. A628, 170 (1998). The numerical results for the $\beta\beta_{0\nu}$ moments in the above mentioned nuclei are similar to those obtained in other theoretical studies although smaller on averag by $\sim 40\%$. We attribute this difference basically to the one-QRPA-method, employed here for the first time, instead of the currently used two-QRPA-method. The difference is partially due to the way of carrying out the restoration of the spin-isospin symmetry. It is hard to say which is the best way to make the restoration, since the $\beta\beta_{0\nu}$ moments are not experimentally measurable. The numerical uncertainties in the $\beta\beta$ moments, related with i) their strong dependence on the residual interaction in the p-p channel when evaluated within the QRPA, and ii) lack of proper knowledge of single-particle energies, have been quantified. It is concluded that the partial restoration of the SU (4) symmetry is crucial in the description of the $\beta\beta$-decays, regardless of the nuclear model used., Comment: 18 pages, 4 figures, accepted for publication in Physical Review C
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- 2017
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11. Study of UV ageing effects in modern artists’ paints with MeV-SIMS
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Krmpotić, Matea, Jembrih-Simbürger, Dubravka, Siketić, Zdravko, Anghelone, Marta, and Radović, Iva Bogdanović
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- 2022
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12. Temporary Urban Interventions in Public Space
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Ivana Krmpotić Romić and Bojana Bojanić Obad Šćitaroci
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public space ,temporary urbanism ,terrain vague ,urban appropriation ,Architecture ,NA1-9428 ,Building construction ,TH1-9745 - Abstract
Insufficient research on the typology of temporary urban interventions, which has become a common tool for pointing out possible scenarios in the development of unused and neglected urban areas in recent 21st century projects, indicate the relevance of the article’s topic. A literature review has allowed for a definition and analysis of the typology of temporary urban interventions in order to determine their basic characteristics, the relationship between the intervention and public space, and the establishing initiatives. The paper determines the formative period through an analysis of architectural and artistic events in 20th century and distinguishes three direct predecessors: events in public space, experimental architectural projects of the 60s, and art installations in public space in the second half of 20th century. A synthesis of collected data is an important prerequisite for understanding the role and impact of temporary urban interventions on future public space. Based on the obtained results, the research emphasizes the importance of the relationship between temporary urban interventions and public space for the creation of contemporary urban space in 21st century.
- Published
- 2022
13. MCMV-based vaccine vectors expressing full-length viral proteins provide long-term humoral immune protection upon a single-shot vaccination
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Kim, Yeonsu, Zheng, Xiaoyan, Eschke, Kathrin, Chaudhry, M. Zeeshan, Bertoglio, Federico, Tomić, Adriana, Krmpotić, Astrid, Hoffmann, Markus, Bar-On, Yotam, Boehme, Julia, Bruder, Dunja, Ebensen, Thomas, Brunotte, Linda, Ludwig, Stephan, Messerle, Martin, Guzman, Carlos, Mandelboim, Ofer, Hust, Michael, Pöhlmann, Stefan, Jonjić, Stipan, and Čičin-Šain, Luka
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- 2022
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14. Systematic study of the [formula omitted]([formula omitted],p)[formula omitted] reaction for NRA applications
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Provatas, G., Fazinić, S., Soić, N., Vukman, N., Cosic, D., Krmpotić, M., Palada, L., Popočovski, R., Dell’Aquila, D., Jakšić, M., Kokkoris, M., and Maragkos, F.
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- 2021
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15. Viral infection of the ovaries compromises pregnancy and reveals innate immune mechanisms protecting fertility
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Tomac, Jelena, Mazor, Marija, Lisnić, Berislav, Golemac, Mijo, Kveštak, Daria, Bralić, Marina, Bilić Zulle, Lidija, Brinkmann, Melanie M., Dölken, Lars, Reinert, Line S., Paludan, Soren R., Krmpotić, Astrid, Jonjić, Stipan, and Juranić Lisnić, Vanda
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- 2021
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16. Relativistic model for the nonmesonic weak decay of single-lambda hypernuclei
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Fontoura, C. E., Krmpotić, F., Galeão, A. P., De Conti, C., and Krein, G.
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Nuclear Theory - Abstract
Having in mind its future extension for theoretical investigations related to charmed nuclei, we develop a relativistic formalism for the nonmesonic weak decay of single-$\Lambda$ hypernuclei in the framework of the independent-particle shell model and with the dynamics represented by the $(\pi,K)$ one-meson-exchange model. Numerical results for the one-nucleon-induced transition rates of ${}^{12}_{\Lambda}\textrm{C}$ are presented and compared with those obtained in the analogous nonrelativistic calculation. There is satisfactory agreement between the two approaches, and the most noteworthy difference is that the ratio $\Gamma_{n}/\Gamma_{p}$ is appreciably higher and closer to the experimental value in the relativistic calculation. Large discrepancies between ours and previous relativistic calculations are found, for which we do not encounter any fully satisfactory explanation. The most recent experimental data is well reproduced by our results. In summary, we have achieved our purpose to develop a reliable model for the relativistic calculation of the nonmesonic weak decay of $\Lambda$-hypernuclei, which can now be extended to evaluate similar processes in charmed nuclei.
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- 2015
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17. MORPHOLOGICAL DIVERSITY OF FACIAL VIBRISSAE IN Chaetophractus vellerosus (MAMMALIA, XENARTHRA, DASYPODIDAE) AND DIFFERENTIAL MECHANOPERCEPTION
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Krmpotic, Cecilia Mariana, Andrés Laube, Pedro Fernando, Barbeito, Claudio Gustavo, Pombo, María Teresa, Scarano, Alejo Carlos, Loza, Cleopatra Mara, and Carlini, Alfredo Armando
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- 2020
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18. Differential cross section measurements of the 9Be(3He,p)11B reaction for NRA applications
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Provatas, G., Fazinić, S., Soić, N., Vukman, N., Cosic, D., Krmpotić, M., Vukšić, M., Crnjac, A., Popočovski, R., Palada, L., Čolović, P., Dell’Aquila, D., Gašparić, I., Malenica, D. Jelavić, Mijatović, T., and Uroić, M.
- Published
- 2020
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19. Nonmesonic Weak Decay Dynamics from proton spectra of $\Lambda$-Hypernuclei
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Krmpotic, Franjo and De Conti, Cláudio
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Nuclear Theory - Abstract
A novel comparison between the data and the theory is proposed for the nonmesonic (NM) weak decay of hypernuclei. Instead of confronting the primary decay rates, as is usually done, we focus attention on the effective decay rates that are straightforwardly related with the number of emitted particles. Proton kinetic energy spectra of $^5_\Lambda$He, $^7_\Lambda$Li, $^9_\Lambda$Be, $^{11}_\Lambda$B, $^{12}_{\Lambda}$C, $^{13}_\Lambda$C, $^{15}_{\Lambda}$N and $^{16}_{\Lambda}$O, measured by FINUDA, are evaluated theoretically. The Independent Particle Shell Model (IPSM) is used as the nuclear structure framework, while the dynamics is described by the One-Meson-Exchange (OME) potential. Only for the $^{5}_{\Lambda}$He, $^{7}_{\Lambda}$Li, and $^{12}_{\Lambda}$C hypernuclei is it possible to make a comparison with the data, since for the rest there is no published experimental information on number of produced hypernuclei. Considering solely the one-nucleon-induced ($1N$-NM) decay channel, the theory reproduces correctly the shapes of all three spectra at medium and high energies ($E_p \geq 40 $ MeV). Yet, it greatly overestimates their magnitudes, as well as the corresponding transition rates when the full OME ($\pi+K+ \eta+\rho+\omega+K^*$) model is used. The agreement is much improved when only the $\pi+K$ mesons with soft dipole cutoff parameters participate in the decay process. We find that the IPSM is a fair first order approximation to disentangle the dynamics of the $1N$-NM decay, the knowledge of which is indispensable to inquire about the baryon-baryon strangeness-flipping interaction. It is shown that the IPSM provides very useful insights regarding the determination the $2N$-NM decay rate. In a new analysis of the FINUDA data, we derive two results for this quantity with one of them close to that obtained previously.
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- 2015
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20. Supporting the improvement of air quality management practices: The “FAIRMODE pilot” activity
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Pisoni, E., Guerreiro, C., Lopez-Aparicio, S., Guevara, M., Tarrason, L., Janssen, S., Thunis, P., Pfäfflin, F., Piersanti, A., Briganti, G., Cappelletti, A., D'Elia, I., Mircea, M., Villani, M.G., Vitali, L., Matavž, L., Rus, M., Žabkar, R., Kauhaniemi, M., Karppinen, A., Kousa, A., Väkevä, O., Eneroth, K., Stortini, M., Delaney, K., Struzewska, J., Durka, P., Kaminski, J.W., Krmpotic, S., Vidic, S., Belavic, M., Brzoja, D., Milic, V., Assimakopoulos, V.D., Fameli, K.M., Polimerova, T., Stoyneva, E., Hristova, Y., Sokolovski, E., and Cuvelier, C.
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- 2019
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21. Adaptations to a semiaquatic lifestyle in the external ear of southern pinnipeds (Otariidae and Phocidae, Carnivora): Morphological evidences
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Loza, Cleopatra M., Krmpotic, Cecilia M., Galliari, Fernando C., Andrés Laube, Pedro F., Negrete, Javier, Scarano, Alejo C., Loureiro, Julio, Carlini, Alfredo A., and Barbeito, Claudio G.
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- 2019
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22. A single-dose MCMV-based vaccine elicits long-lasting immune protection in mice against distinct SARS-CoV-2 variants.
- Author
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Metzdorf, Kristin, Jacobsen, Henning, Yeonsu Kim, Teixeira Alves, Luiz Gustavo, Kulkarni, Upasana, Eschke, Kathrin, Chaudhry, M. Zeeshan, Hoffmann, Markus, Bertoglio, Federico, Ruschig, Maximilian, Hust, Michael, pBrdovčak, Maja Cokarić, Materljan, Jelena, Šustić, Marko, Krmpotić, Astrid, Jonjić, Stipan, Widera, Marek, Ciesek, Sandra, Pöhlmann, Stefan, and Landthaler, Markus
- Subjects
BOOSTER vaccines ,SARS-CoV-2 ,COVID-19 pandemic ,SARS-CoV-2 Omicron variant ,CELLULAR immunity - Abstract
Current vaccines against COVID-19 elicit immune responses that are overall strong but wane rapidly. As a consequence, the necessary booster shots have contributed to vaccine fatigue. Hence, vaccines that would provide lasting protection against COVID-19 are needed, but are still unavailable. Cytomegaloviruses (CMVs) elicit lasting and uniquely strong immune responses. Used as vaccine vectors, they may be attractive tools that obviate the need for boosters. Therefore, we tested the murine CMV (MCMV) as a vaccine vector against COVID-19 in relevant preclinical models of immunization and challenge. We have previously developed a recombinant MCMV vaccine vector expressing the spike protein of the ancestral SARS-CoV-2 (MCMVS). In this study, we show that the MCMVS elicits a robust and lasting protection in young and aged mice. Notably, spike-specific humoral and cellular immunity was not only maintained but also even increased over a period of at least 6 months. During that time, antibody avidity continuously increased and expanded in breadth, resulting in neutralization of genetically distant variants, like Omicron BA.1. A single dose of MCMVS conferred rapid virus clearance upon challenge. Moreover, MCMVS vaccination controlled two variants of concern (VOCs), the Beta (B.1.135) and the Omicron (BA.1) variants. Thus, CMV vectors provide unique advantages over other vaccine technologies, eliciting broadly reactive and long-lasting immune responses against COVID-19. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Myeloid Cells Restrict MCMV and Drive Stress-Induced Extramedullary Hematopoiesis through STAT1
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Gawish, Riem, Bulat, Tanja, Biaggio, Mario, Lassnig, Caroline, Bago-Horvath, Zsuzsanna, Macho-Maschler, Sabine, Poelzl, Andrea, Simonović, Natalija, Prchal-Murphy, Michaela, Rom, Rita, Amenitsch, Lena, Ferrarese, Luca, Kornhoff, Juliana, Lederer, Therese, Svinka, Jasmin, Eferl, Robert, Bosmann, Markus, Kalinke, Ulrich, Stoiber, Dagmar, Sexl, Veronika, Krmpotić, Astrid, Jonjić, Stipan, Müller, Mathias, and Strobl, Birgit
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- 2019
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24. Immune responses to congenital cytomegalovirus infection
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Brizić, Ilija, Hiršl, Lea, Britt, William J., Krmpotić, Astrid, and Jonjić, Stipan
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- 2018
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25. Investigation of key factors in preparation of alpha sources by electrodeposition
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Krmpotić, Matea, Rožmarić, Martina, and Benedik, Ljudmila
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- 2018
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26. Memory CD8 T Cells Generated by Cytomegalovirus Vaccine Vector Expressing NKG2D Ligand Have Effector-Like Phenotype and Distinct Functional Features
- Author
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Marko Šustić, Maja Cokarić Brdovčak, Berislav Lisnić, Jelena Materljan, Vanda Juranić Lisnić, Carmen Rožmanić, Daniela Indenbirken, Lea Hiršl, Dirk H. Busch, Ilija Brizić, Astrid Krmpotić, and Stipan Jonjić
- Subjects
memory T cells ,CD8 T lymphocytes ,cytomegalovirus ,vaccine vector ,tumor vaccine ,Klrg1 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Viral vectors have emerged as a promising alternative to classical vaccines due to their great potential for induction of a potent cellular and humoral immunity. Cytomegalovirus (CMV) is an attractive vaccine vector due to its large genome with many non-essential immunoregulatory genes that can be easily manipulated to modify the immune response. CMV generates a strong antigen-specific CD8 T cell response with a gradual accumulation of these cells in the process called memory inflation. In our previous work, we have constructed a mouse CMV vector expressing NKG2D ligand RAE-1γ in place of its viral inhibitor m152 (RAE-1γMCMV), which proved to be highly attenuated in vivo. Despite attenuation, RAE-1γMCMV induced a substantially stronger CD8 T cell response to vectored antigen than the control vector and provided superior protection against bacterial and tumor challenge. In the present study, we confirmed the enhanced protective capacity of RAE-1γMCMV as a tumor vaccine vector and determined the phenotypical and functional characteristics of memory CD8 T cells induced by the RAE-1γ expressing MCMV. RNAseq data revealed higher transcription of numerous genes associated with effector-like CD8 T cell phenotype in RAE-1γMCMV immunized mice. CD8 T cells primed with RAE-1γMCMV were enriched in TCF1 negative population, with higher expression of KLRG1 and lower expression of CD127, CD27, and Eomes. These phenotypical differences were associated with distinct functional features as cells primed with RAE-1γMCMV showed inferior cytokine-producing abilities but comparable cytotoxic potential. After adoptive transfer into naive hosts, OT-1 cells induced with both RAE-1γMCMV and the control vector were equally efficient in rejecting established tumors, suggesting the context of latent infection and cell numbers as important determinants of enhanced anti-tumor response following RAE-1γMCMV vaccination. Overall, our results shed new light on the phenotypical and functional distinctness of memory CD8 T cells induced with CMV vector expressing cellular ligand for the NKG2D receptor.
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- 2021
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27. The role of tungsten phases formation during tungsten metal powder consolidation by FAST: Implications for high-temperature applications
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Šestan, Andreja, Jenuš, Petra, Krmpotič, Saša Novak, Zavašnik, Janez, and Čeh, Miran
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- 2018
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28. Role of antibodies in confining cytomegalovirus after reactivation from latency: three decades’ résumé
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Krmpotić, Astrid, Podlech, Jürgen, Reddehase, Matthias J., Britt, William J., and Jonjić, Stipan
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- 2019
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29. CD4 T cells are required for maintenance of CD8 TRM cells and virus control in the brain of MCMV-infected newborn mice
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Brizić, Ilija, Hiršl, Lea, Šustić, Marko, Golemac, Mijo, Britt, William J., Krmpotić, Astrid, and Jonjić, Stipan
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- 2019
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30. Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion
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Guillem Angulo, Jelena Zeleznjak, Pablo Martínez-Vicente, Joan Puñet-Ortiz, Hartmut Hengel, Martin Messerle, Annette Oxenius, Stipan Jonjic, Astrid Krmpotić, Pablo Engel, and Ana Angulo
- Subjects
cytomegalovirus ,herpesvirus ,t-cell co-stimulation ,icosl ,cd275 ,viral immune evasion ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Viral infections are controlled, and very often cleared, by activated T lymphocytes. The inducible co-stimulator (ICOS) mediates its functions by binding to its ligand ICOSL, enhancing T-cell activation and optimal germinal center (GC) formation. Here, we show that ICOSL is heavily downmodulated during infection of antigen-presenting cells by different herpesviruses. We found that, in murine cytomegalovirus (MCMV), the immunoevasin m138/fcr-1 physically interacts with ICOSL, impeding its maturation and promoting its lysosomal degradation. This viral protein counteracts T-cell responses, in an ICOS-dependent manner, and limits virus control during the acute MCMV infection. Additionally, we report that blockade of ICOSL in MCMV-infected mice critically regulates the production of MCMV-specific antibodies due to a reduction of T follicular helper and GC B cells. Altogether, these findings reveal a novel mechanism evolved by MCMV to counteract adaptive immune surveillance, and demonstrates a role of the ICOS:ICOSL axis in the host defense against herpesviruses.
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- 2021
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31. Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells
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Rožmanić, Carmen, primary, Lisnić, Berislav, additional, Pribanić Matešić, Marina, additional, Mihalić, Andrea, additional, Hiršl, Lea, additional, Park, Eugene, additional, Lesac Brizić, Ana, additional, Indenbirken, Daniela, additional, Viduka, Ina, additional, Šantić, Marina, additional, Adler, Barbara, additional, Yokoyama, Wayne M., additional, Krmpotić, Astrid, additional, Juranić Lisnić, Vanda, additional, Jonjić, Stipan, additional, and Brizić, Ilija, additional
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- 2023
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32. Xenarthra (Mammalia) from a new late Neogene fossiliferous locality in Northwestern Argentina
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Zurita, Alfredo E., Camacho, María, Miño-Boilini, Angel R., Candela, Adriana M., Cuadrelli, Francisco, Krmpotic, Cecilia M., and Solís, Natalia
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- 2017
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33. Mouse cytomegalovirus encoded immunoevasins and evolution of Ly49 receptors – Sidekicks or enemies?
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Zeleznjak, Jelena, Popovic, Branka, Krmpotic, Astrid, Jonjic, Stipan, and Lisnic, Vanda Juranic
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- 2017
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34. Biochemical and Physiological Responses of Arabidopsis thaliana Leaves to Moderate Mechanical Stimulation
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Šutevski, Iva, Krmpotić, Klara, Vitko, Sandra, Bauer, Nataša, Fancev, Eva, Cifrek, Mario, and Vidaković-Cifrek, Željka
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Physiology ,Plant Science ,Biochemistry ,Arabidopsis thaliana ,mechanical stimulation ,oxidative stress ,antioxidant enzymes ,photosynthetic performance ,systemic response - Abstract
Mechanical stimulation of plants can be caused by various abiotic and biotic environmental factors. Apart from the negative consequences, it can also cause positive changes, such as acclimatization of plants to stress conditions. Therefore, it is necessary to study the physiological and biochemical mechanisms underlying the response of plants to mechanical stimulation. Our aim was to evaluate the response of model plant Arabidopsis thaliana to a moderate force of 5 N (newton) for 20 s, which could be compared with the pressure caused by animal movement and weather conditions such as heavy rain. Mechanically stimulated leaves were sampled 1 h after exposure and after a recovery period of 20 h. To study a possible systemic response, unstimulated leaves of treated plants were collected 20 h after exposure alongside the stimulated leaves from the same plants. The effect of stimulation was assessed by measuring oxidative stress parameters, antioxidant enzymes activity, total phenolics, and photosynthetic performance. Stimulated leaves showed increased lipid peroxidation 1 h after treatment and increased superoxide dismutase activity and phenolic oxidation rate after a 20-h recovery period. Considering photosynthetic performance after the 20-h recovery period, the effective quantum yield of the photosystem II was lower in the stimulated leaves, whereas photochemical quenching was lower in the unstimulated leaves of the treated plants. Nonphotochemical quenching was lower in the stimulated leaves 1 h after treatment. Our study suggested that plants sensed moderate force, but it did not induce pronounced change in metabolism or photosynthetic performance. Principal component analysis distinguished three groups–leaves of untreated plants, leaves analysed 1 h after stimulation, while stimulated and unstimulated leaves of treated plants analysed 20 h after treatment formed together the third group. Observed grouping of stimulated and unstimulated leaves of treated plants could indicate signal transduction from the stimulated to distant leaves, that is, a systemic response to a local application of mechanical stimuli.
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- 2023
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35. A Viral Immunoevasin Controls Innate Immunity by Targeting the Prototypical Natural Killer Cell Receptor Family
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Aguilar, Oscar A., Berry, Richard, Rahim, Mir Munir A., Reichel, Johanna J., Popović, Branka, Tanaka, Miho, Fu, Zhihui, Balaji, Gautham R., Lau, Timothy N.H., Tu, Megan M., Kirkham, Christina L., Mahmoud, Ahmad Bakur, Mesci, Aruz, Krmpotić, Astrid, Allan, David S.J., Makrigiannis, Andrew P., Jonjić, Stipan, Rossjohn, Jamie, and Carlyle, James R.
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- 2017
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36. CD8 T Cell Vaccines and a Cytomegalovirus-Based Vector Approach
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Marko Šustić, Maja Cokarić Brdovčak, Astrid Krmpotić, and Stipan Jonjić
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CD8 T cells ,T-Lymphocytes ,vaccines ,CMV ,cytomegalovirus ,vaccine vector ,Science - Abstract
The twentieth century witnessed a huge expansion in the number of vaccines used with great success in combating diseases, especially the ones caused by viral and bacterial pathogens. Despite this, several major public health threats, such as HIV, tuberculosis, malaria, and cancer, still pose an enormous humanitarian and economic burden. As vaccines based on the induction of protective, neutralizing antibodies have not managed to effectively combat these diseases, in recent decades, the focus has increasingly shifted towards the cellular immune response. There is substantial evidence demonstrating CD8 T cells as key players in the protection not only against many viral and bacterial pathogens, but also in the fight against neoplastic cells. Here, we present arguments for CD8 T cells to be considered as promising candidates for vaccine targeting. We discuss the heterogeneity of CD8 T cell populations and their contribution in the protection of the host. We also outline several strategies of using a common human pathogen, cytomegalovirus, as a vaccine vector since accumulated data strongly suggest it represents a promising approach to the development of novel vaccines against both pathogens and tumors.
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- 2021
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37. Toward Developing Techniques─Agnostic Machine Learning Classification Models for Forensically Relevant Glass Fragments
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Omer Kaspi, Osnat Israelsohn-Azulay, Zidon Yigal, Hila Rosengarten, Matea Krmpotić, Sabrina Gouasmia, Iva Bogdanović Radović, Pasi Jalkanen, Anna Liski, Kenichiro Mizohata, Jyrki Räisänen, Zsolt Kasztovszky, Ildikó Harsányi, Raghunath Acharya, Pradeep K. Pujari, Molnár Mihály, Mihaly Braun, Nahum Shabi, Olga Girshevitz, Hanoch Senderowitz, Materials Physics, and Department of Physics
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Induced breakdown spectroscopy ,Refractive-index ,General Chemical Engineering ,General Chemistry ,Precision ,Library and Information Sciences ,114 Physical sciences ,La-icp-ms ,Computer Science Applications ,PIXE ,LA-ICP-MS ,SEM-EDS ,PIGE ,INAA ,PGAA ,car window glass fragments ,Machine Learning ,Databases ,Forensic chemistry ,Elemental composition ,Discrimination ,Tool ,Plasma-mass spectrometry ,Accuracy - Abstract
Glass fragments found in crime scenes may constitute important forensic evidence when properly analyzed, for example, to determine their origin. This analysis could be greatly helped by having a large and diverse database of glass fragments and by using it for constructing reliable machine learning (ML)-based glass classification models. Ideally, the samples that make up this database should be analyzed by a single accurate and standardized analytical technique. However, due to differences in equipment across laboratories, this is not feasible. With this in mind, in this work, we investigated if and how measurement performed at different laboratories on the same set of glass fragments could be combined in the context of ML. First, we demonstrated that elemental analysis methods such as particle-induced X-ray emission (PIXE), laser ablation induct i v e l y coupled plasma mass spectrometry (LA-ICP-MS), scanning electron microscopy with energy-dispersive X-ray spectrometry (SEM-EDS), particle-induced Gamma-ray emission (PIGE), instrumental neutron activation analysis (INAA), and prompt Gamma-ray neutron activation analysis (PGAA) could each produce lab-specific ML-based classification models. Next, we determined rules for the successf u l combinations of data from different laboratories and techniques and demonstrated that when followed, they give rise to improved models, and conversely, poor combinations wi l l lead to poor-performing models. Thus, the combination of PIXE and LA-ICP-MS improves the performances by similar to 10-15%, while combining PGAA with other techniques provides poorer performances in comparison with the lab-specific models. Finally, we demonstrated that the poor performances of the SEM-EDS technique, sti l l in use by law enforcement agencies, could be greatly improved by replacing SEM-EDS measurements for Fe and Ca by PIX E measurements for these elements. These findings suggest a process whereby forensic laboratories using different elemental analysis techniques could upload their data into a unified database and get reliable classification based on lab-agnostic models. This in tur n brings us closer to a more exhaustive extraction of information from glass fragment evidence and furthermore may form the basis for international-wide collaboration between law enforcement agencies.
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- 2022
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38. Erektilna disfunkcija
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Krmpotić, Mihaela, Markić, Dean, Sotošek, Stanislav, Ahel, Juraj, and Španjol, Josip
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erectile dysfunction ,sexual activity ,PDE5 inhibitors - Abstract
Erektilna disfunkcija je trajna nemogućnost postizanja i održavanja erekcije dostatne za zadovoljavajuću spolnu aktivnost. Istraživanja pokazuju da preko 50% muškaraca u dobi od 40 do 70 godina pati od ED-a, no točan broj teško je procijeniti s obzirom da se mnogi muškarci rijetko javljaju liječniku radi navedenog problema. ED se etiološki može klasificirati na psihogenu, organsku, i mješovitu. Organska ED uključuje vaskulogenu, neurogenu, endokrinološku i medikamentoznu etiologiju. ED uzrokuje pad kvalitete života pacijenta i njegovog partnera. Stres i depresija često prate bolest pa s vremenom skoro sve organske disfunkcije najčešće postaju miješane. Najčešći čimbenici rizika za ED su hipertenzija, dijabetes melitus, pušenje, dislipidemije, pretilost, fizička neaktivnost i stres. Osnovni postupci pri dijagnosticiranju ED-a obuhvaćaju temeljitu anamnezu, fizikalni pregled, hormonske i biokemijske analize te procjenu psihološkog profila pacijenta. Kod nekih pacijenata bit će potrebne daljnje pretrage. Prva linija terapije ED-a obuhvaća promjenu životnih navika, farmakoterapiju oralnim inhibitorima PDE5 te korištenje vakuumskih uređaja za erekciju. U slučaju nedjelotvornosti prve linije terapije, kao sekundarna linija koriste se intrakavernozne injekcije ili intrauretralni supozitoriji alprostadila. U tercijarnu liniju terapije spadaju kirurške metode., Erectile dysfunction is defined as the inability to achieve and maintain an erection sufficient for satisfactory sexual activity. Research shows that over 50% of men between the ages of 40 and 70 suffer from ED, but the exact number is difficult to estimate, given that many men rarely seek medical help about the problem. ED can be etiologically classified into psychogenic, organic, and mixed ED. Organic ED includes vasculogenic, neurogenic, endocrinological and medicamentous etiologies. ED causes a decline in the quality of life of the patient and his partner. Stress and depression often accompany ED, so over time almost all organic dysfunctions usually become mixed. The most common risk factors for ED are hypertension, diabetes mellitus, smoking, dyslipidemia, obesity, physical inactivity and stress. Basic procedures for diagnosing ED include a thorough medical history, physical examination, hormonal and biochemical analyses, and assessment of the patient's psychological profile. Some patients will require further testing. The first-line therapy for ED includes lifestyle changes, pharmacotherapy with oral PDE5 inhibitors and the use of vacuum-assisted erection devices. In case that the first-line therapy isn't effective, intracavernous injections of alprostadil or intraurethral alprostadil suppositories are used as second-line therapy. The third-line therapy includes surgical methods.
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- 2023
39. Advancements of ELBE Timing System Upgrade
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(0000-0002-8145-5837) Kuntzsch, M., (0000-0002-8120-6806) Zenker, K., Schwarz, A., Justus, M., Hrovatin, R., Peruško, L., Legat, U., Rojec, U., Oven, Z., Krmpotić, L., (0000-0002-8145-5837) Kuntzsch, M., (0000-0002-8120-6806) Zenker, K., Schwarz, A., Justus, M., Hrovatin, R., Peruško, L., Legat, U., Rojec, U., Oven, Z., and Krmpotić, L.
- Abstract
Modern Big physics experiments call for optimizations of machines in various aspects. Integration of an advanced control system is one of them, and timing system as controls’ backbone is most often required to be upgraded significantly or even designed and implemented anew. The complexity of experiments at HZDR ELBE and the range of varieties of its instruments and subsystems is combined with top-notch performance requirements. These, coupled with hardware obsolescence, dictate an implementation of an advanced timing system, surpassing a system, that was designed initially. The new timing system must be compatible with all existing timing triggering patterns and must provide configuration option for new features. It is designed to generate trigger patterns for shot on demand to 26 MHz cw which requires a universal and complex implementation of the pattern composition and validity checks. The design of this timing solution further demands the adaptation and the modification of the event-based timing system built on MRF HW. As a result, we realized the new Control Software with an extended range of functionalities. While maintaining the common functionality we made it suitable for the most demanding experiments today.
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- 2023
40. Izazovi i uspjesi udruge CroMSIC u sferi volontiranja, projekata i razmjena
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Radošević, Mia, Radoš, Laura, Vareško, Aurora, Paradinović, Lea, Kovačević Žižić, Mia, Ban, Adriana, Klepač, Lea, Cahun, Elena, Miljanić, Klara, Selimović, Tiyya, Žujić, Dino, Galić, Petra, Smojver, Ivan, Suknović, Mia, Pervan, Matij, Ercegović, Vladimir, Kukić, Sandro, Halić Kordić, Kana, Rudelj, Laura, Krmpotić, Angelina, Radošević, Mia, Radoš, Laura, Vareško, Aurora, Paradinović, Lea, Kovačević Žižić, Mia, Ban, Adriana, Klepač, Lea, Cahun, Elena, Miljanić, Klara, Selimović, Tiyya, Žujić, Dino, Galić, Petra, Smojver, Ivan, Suknović, Mia, Pervan, Matij, Ercegović, Vladimir, Kukić, Sandro, Halić Kordić, Kana, Rudelj, Laura, and Krmpotić, Angelina
- Abstract
Međunarodna udruga studenata medicine Hrvatska – CroMSIC jest udruga studenata medicine posvećena poboljšanju obrazovanja i suradnje diljem Republike Hrvatske. Djelovanje udruge ostvaruje se kroz volontiranje studenata. Udruga provodi niz projekata usmjerenih na prevenciju i edukaciju, uključujući akcije s tematikom iz područja javnog zdravstva, ljudskih prava, spolnog i reproduktivnog zdravlja te medicinske edukacije. Također, provodi program studentskih razmjena kojim omogućavamo studentima putovanja te profesionalne i znanstvene razmjene diljem svijeta, što pridonosi prepoznavanju Republike Hrvatske, kao i hrvatskih sveučilišta na svjetskoj razini. CroMSIC pruža jedinstveno međunarodno iskustvo i priliku za osobni i profesionalni razvoj. Također kroz razne projekte i akcije, iskazujemo svoju humanitarnu stranu pomažući djeci i obiteljima u potrebi, dok naši projekti omogućavaju zdravstvenu skrb u ruralnim područjima koja su često zapostavljena. Naši predani dužnosnici bave se različitim aspektima zdravstva, uključujući specifične teme poput prevencije raka i genetike u suradnji s lokalnim zajednicama i stručnjacima. CroMSIC pokreće pozitivne promjene kroz suradnju sa školama, domovima za djecu i lokalnim zajednicama. Naši dosadašnji uspjesi uključuju obrazovanje tisuća učenika i sudjelovanje na stotine studenata volontera u projektima. Cilj je ovog članka prikazati udrugu CroMSIC i njezine ciljeve – pridonijeti boljem zdravlju i obrazovanju u Republici Hrvatskoj te oblikovati buduće generacije medicinskih stručnjaka koji će donositi pozitivne promjene u društvu., The Croatian Medical Students’ International Committee (CroMSIC) is an association dedicated to improving education and collaboration across the Republic of Croatia. The organization’s activities are carried out through student volunteering. It implements a range of projects focused on prevention and education, including initiatives in public health, human rights, sexual and reproductive health, as well as medical education. Additionally, we conduct student exchange programs that enable students to travel and participate in professional and scientific exchanges worldwide, contributing to the recognition of Croatia and its university on a global scale. CroMSIC, as an association, provides a unique international experience and an opportunity for personal and professional development. Through various projects and initiatives, CroMSIC demonstrates its humanitarian side by assisting children and families in need, while some projects facilitate healthcare in often neglected rural areas. Their dedicated officials also work to raise awareness about various healthcare aspects, including specific topics like cancer prevention and genetics, in collaboration with local communities and experts. CroMSIC drives positive change through cooperation with schools, children’s homes, and local communities. Through its projects, the association aims to enhance the quality of medical education and raise awareness about key public health issues. Our past successes include educating thousands of students and engaging hundreds of student volunteers in projects. The aim of this article is to present the association CroMSIC as well as its goals – to contribute to better health and education in the Republic of Croatia, and to shape future generations of medical experts who will bring positive changes in society.
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- 2023
41. Future Public Spaces; Urban Design in Times of Crisis
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Krmpotić Romić, Ivana and Krmpotić Romić, Ivana
- Published
- 2023
42. A genetic history of the Balkans from Roman frontier to Slavic migrations
- Author
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Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Fundación la Caixa, Natural Sciences and Engineering Research Council of Canada, Ministry of Science, Education and Sports of the Republic of Croatia, National Institutes of Health (US), John Templeton Foundation, Paul G. Allen Family Foundation, Howard Hughes Medical Institute, Olalde, Iñigo [0000-0002-2660-6807], Lalueza-Fox, Carles [0000-0002-1730-5914], Olalde, Iñigo, Carrión, Pablo, Mikić, Ilija, Rohland, Nadin, Mallick, Swapan, Lazaridis, Iosif, Mah, Matthew, Miomir Korać, Golubović, Snežana, Petković, Sofija, Miladinović-Radmilović, Nataša, Vulović, Dragana, Alihodžić, Timka, Ash, Abigail, Baeta, Miriam, Bartík, Juraj, Bedić, Željka, Bilić, Maja, Bonsall, Clive, Bunčić, Maja, Bužanić, Domagoj, Carić, Mario, Čataj, Lea, Cvetko, Mirna, Drnić, Ivan, Dugonjić, Anita, Đukić, Ana, Đukić, Ksenija, Farkaš, Zdeněk, Jelínek, Pavol, Jovanovic, Marija, Kaić, Iva, Kalafatić, Hrvoje, Krmpotić, Marijana, Krznar, Siniša, Leleković, Tino, Pancorbo, Marian M. de, Matijević, Vinka, Milošević Zakić, Branka, Osterholtz, Anna J., Paige, Julianne M., Tresić Pavičić, Dinko, Premužić, Zrinka, Rajić Šikanjić, Petra, Rapan Papeša, Anita, Paraman, Lujana, Sanader, Mirjana, Radovanović, Ivana, Roksandic, Mirjana, Šefčáková, Alena, Stefanović, Sofia, Teschler-Nicola, Maria, Tončinić, Domagoj, Zagorc, Brina, Callan, Kim, Candilio, Francesca, Cheronet, Olivia, Fernandes, Daniel, Kearns, Aisling, Lawson, Ann Marie, Mandl, Kirsten, Wagner, Anna, Zalzala, Fatma, Zettl, Anna, Tomanović, Željko, Keckarević, Dušan, Novak, Mario, Harper, Kyle, McCormick, Michael, Pinhasi, Ron, Grbić, Miodrag, Lalueza-Fox, Carles, Reich, David, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Fundación la Caixa, Natural Sciences and Engineering Research Council of Canada, Ministry of Science, Education and Sports of the Republic of Croatia, National Institutes of Health (US), John Templeton Foundation, Paul G. Allen Family Foundation, Howard Hughes Medical Institute, Olalde, Iñigo [0000-0002-2660-6807], Lalueza-Fox, Carles [0000-0002-1730-5914], Olalde, Iñigo, Carrión, Pablo, Mikić, Ilija, Rohland, Nadin, Mallick, Swapan, Lazaridis, Iosif, Mah, Matthew, Miomir Korać, Golubović, Snežana, Petković, Sofija, Miladinović-Radmilović, Nataša, Vulović, Dragana, Alihodžić, Timka, Ash, Abigail, Baeta, Miriam, Bartík, Juraj, Bedić, Željka, Bilić, Maja, Bonsall, Clive, Bunčić, Maja, Bužanić, Domagoj, Carić, Mario, Čataj, Lea, Cvetko, Mirna, Drnić, Ivan, Dugonjić, Anita, Đukić, Ana, Đukić, Ksenija, Farkaš, Zdeněk, Jelínek, Pavol, Jovanovic, Marija, Kaić, Iva, Kalafatić, Hrvoje, Krmpotić, Marijana, Krznar, Siniša, Leleković, Tino, Pancorbo, Marian M. de, Matijević, Vinka, Milošević Zakić, Branka, Osterholtz, Anna J., Paige, Julianne M., Tresić Pavičić, Dinko, Premužić, Zrinka, Rajić Šikanjić, Petra, Rapan Papeša, Anita, Paraman, Lujana, Sanader, Mirjana, Radovanović, Ivana, Roksandic, Mirjana, Šefčáková, Alena, Stefanović, Sofia, Teschler-Nicola, Maria, Tončinić, Domagoj, Zagorc, Brina, Callan, Kim, Candilio, Francesca, Cheronet, Olivia, Fernandes, Daniel, Kearns, Aisling, Lawson, Ann Marie, Mandl, Kirsten, Wagner, Anna, Zalzala, Fatma, Zettl, Anna, Tomanović, Željko, Keckarević, Dušan, Novak, Mario, Harper, Kyle, McCormick, Michael, Pinhasi, Ron, Grbić, Miodrag, Lalueza-Fox, Carles, and Reich, David
- Abstract
[Summary] The rise and fall of the Roman Empire was a socio-political process with enormous ramifications for human history. The Middle Danube was a crucial frontier and a crossroads for population and cultural movement. Here, we present genome-wide data from 136 Balkan individuals dated to the 1st millennium CE. Despite extensive militarization and cultural influence, we find little ancestry contribution from peoples of Italic descent. However, we trace a large-scale influx of people of Anatolian ancestry during the Imperial period. Between ∼250 and 550 CE, we detect migrants with ancestry from Central/Northern Europe and the Steppe, confirming that “barbarian” migrations were propelled by ethnically diverse confederations. Following the end of Roman control, we detect the large-scale arrival of individuals who were genetically similar to modern Eastern European Slavic-speaking populations, who contributed 30%–60% of the ancestry of Balkan people, representing one of the largest permanent demographic changes anywhere in Europe during the Migration Period.
- Published
- 2023
43. Mussels (Mytilus galloprovincialis) as a bio-indicator species in radioactivity monitoring of Eastern Adriatic coastal waters
- Author
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Krmpotić, Matea, Rožmarić, Martina, and Barišić, Delko
- Published
- 2015
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44. HISTORICAL DERIVATION OF THE PROTO-SLAVIC PRESENT STEM. THE INDO-EUROPEAN HERITAGE AND DEVELOPMENT
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Krmpotić, Pavao, Matasović, Ranko, Stolac, Diana, Kapović, Mate, Snoj, Marko, and Zubčić, Sanja
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accentology ,811.163.42 ,Proto-Slavic ,etymology ,diachronic word formation ,present stem - Abstract
U radu se donose rezultati istraživanja povijesne tvorbe prezentske glagolske osnove u praslavenskome jeziku. Rad se zasniva na suvremenoj indoeuropeističkoj i slavističkoj literaturi te na vlastitoj interpretaciji pojedinih primjera prezentskih osnova ekscerpiranih iz prikupljene građe. Za svaki primjer praslavenske prezentske osnove navode se najvažniji podatci: etimologija, akcentuacija te potvrde iz slavenskih jezika s posebnim naglaskom na građu iz hrvatskih dijalekata. Na istraživačka pitanja postavljena u uvodnome dijelu rada pokušava se odgovoriti u zaključku nakon što smo u središnjem dijelu rada analizirali tvorbu pojedinačnih praslavenskih prezentskih osnova. Istraživačka pitanja vezana su uz baltoslavensko nasljeđe praslavenskih prezentskih osnova i posebnosti u razvoju hrvatskih dijalekata., The aim of this work is to present our research in diachronic derivation of the Proto-Slavic present stem. The work is based on relevant linguistic bibliography and primary sources of the Slavic dialects. The first part of the dissertation presents a theoretical and methodological overview of the verbal morphology in the Indo-European and Balto-Slavic proto-language. We tried to formulate the three major problems regarding the Balto-Slavic verbal derivation, the ProtoSlavic development and attestations of the Proto-Slavic present stems in the contemporary Croatian dialects. A major part of the work is dedicated to the analysis of the Proto-Slavic present stems. Every single Proto-Slavic present stem is described regarding it's etymology, attestation, accentuation and historical derivation.
- Published
- 2023
45. Murine CMV Expressing the High Affinity NKG2D Ligand MULT-1: A Model for the Development of Cytomegalovirus-Based Vaccines
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Lea Hiršl, Ilija Brizić, Tina Jenuš, Vanda Juranić Lisnić, Johanna Julia Reichel, Slaven Jurković, Astrid Krmpotić, and Stipan Jonjić
- Subjects
murine CMV ,cytomegalovirus ,vaccine ,NKG2D ,murine UL16 binding protein-like transcript 1 ,NK cells ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The development of a vaccine against human cytomegalovirus (CMV) has been a subject of long-term medical interest. The research during recent years identified CMV as an attractive vaccine vector against infectious diseases and tumors. The immune response to CMV persists over a lifetime and its unique feature is the inflationary T cell response to certain viral epitopes. CMV encodes numerous genes involved in immunoevasion, which are non-essential for virus growth in vitro. The deletion of those genes results in virus attenuation in vivo, which enables us to dramatically manipulate its virulence and the immune response. We have previously shown that the murine CMV (MCMV) expressing RAE-1γ, one of the cellular ligands for the NKG2D receptor, is highly attenuated in vivo but retains the ability to induce a strong CD8+ T cell response. Here, we demonstrate that recombinant MCMV expressing high affinity NKG2D ligand murine UL16 binding protein-like transcript (MULT-1) (MULT-1MCMV) inserted in the place of its viral inhibitor is dramatically attenuated in vivo in a NK cell-dependent manner, both in immunocompetent adult mice and in immunologically immature newborns. MULT-1MCMV was more attenuated than the recombinant virus expressing RAE-1γ. Despite the drastic sensitivity to innate immune control, MULT-1MCMV induced an efficient CD8+ T cell response to viral and vectored antigens. By using in vitro assay, we showed that similar to RAE-1γMCMV, MULT-1 expressing virus provided strong priming of CD8+ T cells. Moreover, MULT-1MCMV was able to induce anti-viral antibodies, which after passing the transplacental barrier protect offspring of immunized mothers from challenge infection. Altogether, this study further supports the concept that CMV expressing NKG2D ligand possesses excellent characteristics to serve as a vaccine or vaccine vector.
- Published
- 2018
- Full Text
- View/download PDF
46. Concrete production and quality control at concrete plant Apolonia d.o.o
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Krmpotić, Nera, Netinger Grubeša, Ivanka, Miličević, Ivana, and Hadzima-Nyarko, Marijana
- Subjects
production control ,concrete ,quality control - Abstract
Beton je materijal čija je primjena vrlo široka što ga čini jednim od vodećih i najrasprostranjenijih materijala današnjice. Koristi se u zgradarstvu, u izgradnji mostova, kanala, brana, tunela i slično. Proces dobivanja betona je vrlo složen jer zahtijeva proizvodnju u kontroliranim uvjetima i prema točno određenim pravilima propisanih normom za proizvodnju i kontrolu kvalitete betona. U radu je popraćen proizvodni proces izrade i kontrole kvalitete u lokalnoj betonari Apolonia d.o.o. Praćena je proizvodnja betona od njegova naručivanja od strane kupca, tijek proizvodnje, te otprema naručenog betona na zahtijevanu adresu. Također, popraćena su i laboratorijska ispitivanja proizvedenog betona koja se provode u svrhu kontrole njegove kakvoće., Concrete is a material whose application is very broad, which makes it one of the leading and most widespread materials nowadays. It is used in the construction industry, in the construction of bridges, canals, dams, tunnels and such. The process of making concrete is very complex because it requires production under controlled conditions and according to the exact rules prescribed by the norm for the production and quality control of concrete. The paper follows the production process and quality control in the local concrete plant Apolonia d.o.o. The production of concrete was monitored from the time it was ordered by the customer, during the production process,to the delivery of the ordered concrete to the required address. Also, laboratory tests of the produced concrete, which are carried out for the purpose of quality control, are also accompanied.
- Published
- 2023
47. Klub mladih 'o čitanju i svemu pomalo' Gradske knjižnice i čitaonice Virovitica
- Author
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Kotromanović, Mirjana and Krmpotić, Anja
- Subjects
programi za mlade, Gradska knjižnica i čitaonica Virovitica, čitateljski klubovi, klub mladih ,programs for young people, City Library of Virovitica, reading clubs, youth club - Abstract
U sklopu programa za mlade Gradske knjižnice i čitaonice Virovitica djeluje Klub mladih „O čitanju i svemu pomalo“. Članovi spomenutog Kluba, na susretima koji se održavaju jednom mjesečno, razgovaraju o pročitanim knjigama te kreiraju i prezentiraju različite sadržaje za mlade. Cilj je u ovome radu prikazati na koji način djeluje Klub, kakvi su sadržaji kreirani unutar djelovanja Kluba, a u svrhu popularizacije programa za mlade i poticanja na osnivanje čitateljskih klubova za mlade u drugim knjižnicama. U uvodnom dijelu rada riječ je o mladima i čitanju, potrebi osnivanja čitateljskih klubova za mlade te osnivanju Kluba mladih „O čitanju i svemu pomalo“ Gradske knjižnice i čitaonice Virovitica. U središnjem dijelu rada predstavljeni su programi za mlade koji su se u sklopu djelovanja Kluba mladih Gradske knjižnice i čitaonice Virovitica provodili kroz dvije godine. Zaključak donosi promišljanja o potrebi razvijanja čitanja među mladima te kako se kroz djelovanje Klub mladih „O čitanju i svemu pomalo“ povećao broj mladih korisnika u Gradskoj knjižnici i čitaonici Virovitica., As part of the program for young people of the City Library of Virovitica, there is a Youth Club “On reading and all the other things”. At meetings held once a month, members of the aforementioned Club discuss the books they have read and create and present various content for young people. The goal of this work is to show how the Club works, what content is created within the Club’s activities, and for the purpose of popularizing programs for young people and encouraging the establishment of reading clubs for young people in other libraries. In the introductory part of the paper, it is about young people and reading, the need to establish reading clubs for young people, and the establishment of the Youth Club “About reading and everything a little bit” of the City Library of Virovitica. In the central part of the work, programs for young people were presented, which were implemented over two years as part of the activities of the Youth Club of the City Library of Virovitica. The conclusion brings reflections on the need for developing reading among young people and how the number of young users in the City Library of Virovitica increased through the activities of the Youth Club “On reading and all the other things”.
- Published
- 2023
48. Humanitarian Demining Using Data Observatories and Data Lakes
- Author
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Horvat, Marko, Krtalić, Andrija, Akagić, Amila, Krmpotić, Klara, Skender, Sven, and Markt, Katarina
- Subjects
data transformation, data observatory, big data, non-technical survey, mine scene analysis, artificial intelligence - Abstract
Humanitarian demining is an indispensable part of any post-conflict reconstruction and rehabilitation efforts, as landmines and other unexploded ordnance (UXO) continue to pose a significant threat to civilians and infrastructure. Traditional non- technical survey demining methods are typically time-consuming, labor-intensive, and require professional familiarity with a number of different complex tools and involved procedures. The emergence of the latest artificial intelligence (AI) technologies for deep learning (DL) and data analysis techniques presents an opportunity to transform humanitarian demining by significantly improving its efficiency, speed, accuracy, and safety. One such approach is the development of a data observatory that implements data ingestion pipelines into a data lake using data transformation to improve the usability of unstructured data about a mine scene. This paper addresses the key characteristics and benefits of such a system and its potential to improve humanitarian demining.
- Published
- 2023
49. The herpesviral antagonist m152 reveals differential activation of STING‐dependent IRF and NF‐κB signaling and STING's dual role during MCMV infection
- Author
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Stempel, Markus, Chan, Baca, Juranić Lisnić, Vanda, Krmpotić, Astrid, Hartung, Josephine, Paludan, Søren R, Füllbrunn, Nadia, Lemmermann, Niels AW, and Brinkmann, Melanie M
- Published
- 2019
- Full Text
- View/download PDF
50. Temporary Urban Interventions in Public Space
- Author
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Krmpotić Romić, Ivana, primary and Bojanić Obad Šćitaroci, Bojana, additional
- Published
- 2022
- Full Text
- View/download PDF
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