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In order to solve the problem of concrete shaft support in deep topsoil layer, ANSYS finite element software was used to compare and analyze the deformation and stress of hybrid fiber high-strength concrete shaft and ordinary high-strength concrete shaft when the thickness of topsoil layer varied from 200 m to 600 m. The calculation results show that: the strain of mixed fiber concrete shaft is greater than that of ordinary concrete shaft under the same stress, and the fiber can improve the brittleness of shaft; the maximum circumferential stress on shaft increases with the increase of topsoil layer thickness, and increases gradually from outside to inside along the shaft radial direction, and the circumferential strain is always compressive strain and the inner surface is greater than the outer surface. As the burial depth increases, the deformation of shaft changes from mainly axial deformation to mainly radial deformation; finally, the regression equation of the relationship between stress-strain and thickness of topsoil layer is given, which can guide the engineering practice.

Acute pancreatitis (AP) is an inflammatory disease with high morbidity and mortality. The regulation mechanism of miRNA is involved in the production and development of various diseases, but the regulation mechanism of miRNA in AP is still not fully elucidated. The expression of miR-339-3p was detected using quantitative real-time PCR. The levels of TNF-α, IL-1β, and IL-6 were detected using enzyme-linked immunosorbent assay. Cell apoptosis was measured using flow cytometry. The protein expressions of TNF receptor-associated factor 3 (TRAF3), Bcl-2, C-caspase 3, Bax, p-p38, and p38 were measured using western blot. Luciferase reporter assay and RNA immunoprecipitation assay were applied to ensure that miR-399-3p targeted TRAF3. Caerulein promoted the expression of TNF-α, IL-1β, and IL-6, enhanced the expression of C-caspase 3 and Bax while inhibited Bcl-2 protein expression. Meanwhile, caerulein also reduced the expression of miR-339-3p and induced the expression of TRAF3 in rat pancreatic acinar cells. miR-399-3p transfection inhibited the levels of TNF-α, IL-1β, and IL-6 and C-caspase 3 and Bax protein expression as well as suppressed cell apoptosis, while increased Bcl-2 protein expression in caerulein-induced AP. TRAF3 has been verified as a target of miR-339-3p. Interestingly, the reduction of miR-399-3p inhibited the p38 pathway, which was impaired by the upregulation of TRAF3. In addition, the suppression effects of miR-339-3p on cell inflammation and apoptosis in caerulein-induced AP were reversed by enhancing TRAF3 expression. In this study, in vitro model of AP was characterized by strong inflammation and cell apoptosis. We have first demonstrated the regulatory network of miR-339-3p and TRAF3. Overexpression of miR-339-3p inhibited cell inflammation and cell apoptosis in caerulein-induced AP through modulating TRAF3 expression via the p38 pathway, providing a new therapeutic target in the treatment of AP.

Recent advances in the research of late transition metal (Ni) salicylaldimine bimetallic catalysts in ethylene polymerization and copolymerization of ethylene with comonomers have been introduced. The effect of positioning two polymerization-active metal centers in close proximity on catalytic activity, molecular weight, molecular weight distribution, and levels of branching are thoroughly documented. The synergistic effect of these binuclear catalysts compared to their mononuclear analogs is the subject of this review.

In the Cyber-Physical system(CPS), equipment generates a large amount of communication information and equipment data during the operation of the system, which are at risk of being modified and stolen. Block chain technology with distributed storage as the core is conducive to ensuring the data security of CPS system, but the existing consensus algorithms have the problem that data recording rights are concentrated in a few nodes and are easy to be hijacked by malicious nodes. By introducing node validity, the dynamic change of node behavior can be reflected. The experimental results show that the consensus mechanism based on the validity of nodes makes the distribution of nodes with access to data records more decentralized, better reflects the de-centralization characteristics of the system, and improves the security of data.

Carcinogen exposure has been associated with enhanced cancer immunogenicity that is often attributed to neoantigen generation. However, the broader, neoantigen-independent impact of carcinogens on immune responses to cancer cells remains underexplored. In this issue of the JCI, Huang et al. uncover a mechanism wherein carcinogen-treated cancer cells exhibit an inability to establish an immunosuppressive tumor microenvironment (TME) due to reduced M-CSF expression. Intriguingly, the so-called carcinogen-induced tumor-associated macrophages (TAMs) within this TME exhibited anti-tumor properties instead of the conventional immunosuppressive phenotype. This phenomenon extended to human lung cancers, as evidenced by TAM reprogramming in smokers versus nonsmokers. This study substantially advances our understanding of carcinogen-mediated effects on cancer immunogenicity, potentially redirecting approaches to cancer immunotherapy., Carcinogen treatment augments cancer immunogenicity beyond neoantigens Carcinogens are substances with the ability to cause cancer mainly by generating mutations in cellular DNA. Carcinogens responsible for a substantial number of [...]