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Your search keyword '"Lai, Wei-Hua"' showing total 29 results
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29 results on '"Lai, Wei-Hua"'

16. Synergistic Dual-Mechanism Fluorescence Quenching Immunochromatographic Assay Based on Fe(Iii)-Polydopamine Nanobead for Sensitive Detection of Enrofloxacin

Author

Lai, Xiaocui, primary, Zhang, Ganggang, additional, Deng, Shengliang, additional, Huang, Zhen, additional, Peng, Juan, additional, Zhang, Gan, additional, Su, Liu, additional, He, Weihua, additional, Wu, Youlin, additional, Ding, Nengshui, additional, Zhang, Zhigang, additional, and Lai, Wei-Hua, additional

Published
2022
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17. Free Triiodothyronine Connected With Metabolic Changes in Patients With Coronary Artery Disease by Interacting With Other Functional Indicators

Author

Tian, Xiao-xue, primary, Zheng, Shu-fen, additional, Liu, Ju-e, additional, Wu, Yuan-yuan, additional, Lin, Lu, additional, Chen, Hong-mei, additional, Li, Li-wen, additional, Qin, Min, additional, Wang, Zi-xian, additional, Zhu, Qian, additional, Lai, Wei-hua, additional, and Zhong, Shilong, additional

Published
2021
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23. Lateral flow immunoassay integrated with competitive and sandwich models for the detection of aflatoxin M1 and Escherichia coli O157:H7 in milk

Author

Wang, Chun, primary, Peng, Juan, additional, Liu, Dao-Feng, additional, Xing, Ke-Yu, additional, Zhang, Gang-Gang, additional, Huang, Zhen, additional, Cheng, Song, additional, Zhu, Fang-Fei, additional, Duan, Miao-Lin, additional, Zhang, Kai-Yi, additional, Yuan, Mei-Fang, additional, and Lai, Wei-Hua, additional

Published
2018
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29. Lateral flow immunoassay integrated with competitive and sandwich models for the detection of aflatoxin M 1 and Escherichia coli O157:H7 in milk.

Author

Wang C, Peng J, Liu DF, Xing KY, Zhang GG, Huang Z, Cheng S, Zhu FF, Duan ML, Zhang KY, Yuan MF, and Lai WH

Subjects
Animals, Food Microbiology, Gold, Metal Nanoparticles, Aflatoxin M1 isolation & purification, Escherichia coli O157 isolation & purification, Immunoassay methods, Milk chemistry, Milk microbiology
Abstract

Pathogens, mycotoxins, or antibiotics may exist in a food sample. Micro- and macromolecular substances must be detected quickly. A rapid and convenient lateral flow immunoassay (LFI) integrated with competitive and sandwich models was developed to detect micro- and macromolecular substances. In this study, aflatoxin M 1 (AFM 1 ) and Escherichia coli O157:H7 were selected as the micro- and macromolecular substances, respectively. Two test lines in the LFI test strip were evaluated to detect AFM1 and E. coli O157:H7 by competitive and sandwich models. Results showed that the limits of detection for detecting AFM1 and E. coli O157:H7 were 50 pg·mL -1 and 1.58 × 10 4 cfu·mL -1 , respectively. The whole assay time was 30 min. The recoveries of gold nanoparticle-LFI ranged from 78.0 to 111.6% with coefficients of variation in the range of 3.9 to 8.5% for the detection of AFM 1 . For the detection of E. coli O157:H7, the range of recoveries was from 70.1 to 89.6% with coefficients of variation ranging from 4.9 to 13.0%. This study not only tested sensitivity and specificity, but also was a systematic study of location of 2 test lines of the LFI test strip integrated with competitive and sandwich models., (Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Published
2018
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