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222 results on '"Lim, Ki-Byung"'

21. The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids.

Author

Park, Hye-Wan, Sevilleno, Samantha Serafin, Ha, My Khanh Tran Thi, Cabahug-Braza, Raisa Aone, Yi, Ji-Hun, Lim, Ki-Byung, Cho, Wonwoo, and Hwang, Yoon-Jung

Subjects
FLUORESCENCE in situ hybridization, GENITALIA, PLANT breeding, GENETIC variation, FLUORESCENCE, CHROMOSOMES, INTROGRESSION (Genetics)
Abstract

Fluorescence in situ hybridization (FISH), a molecular cytogenetic technique that enables the visualization and identification of specific DNA sequences within chromosomes, has emerged as a pivotal tool in plant breeding programs, particularly in the case of Veronica species. Veronica, a genus with a complex reproductive system, often poses challenges in accurately identifying hybrids because of its tendency to hybridize, which leads to intricate genetic variation. This study focused on the use of FISH as a prescreening method to identify true hybrids in Veronica breeding programs. FISH analysis was first performed on the parents to identify their 45S and 5S rDNA signals, along with their respective chromosome numbers. The signals were then compared with those of the twenty progenies with reference to their supposed parents. Five true hybrids, seven self-pollinated progenies, and eight false hybrids were identified through FISH. The findings highlight the significance of FISH as a screening method that contributes significantly to the efficiency of Veronica breeding programs by ensuring the preservation of desired genetic traits and minimizing the inadvertent inclusion of misidentified hybrids. To conclude, this study underscores the vital role of FISH in enhancing the precision and success of breeding programs and opens new avenues for improved breeding strategies and crop development. [ABSTRACT FROM AUTHOR]

Published
2024
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24. Lilium

Author

Van Tuyl, Jaap M., Arens, Paul, Shahin, Arwa, Marasek-Ciołakowska, Agnieszka, Barba-Gonzalez, Rodrigo, Kim, Hyoung Tae, Lim, Ki-Byung, Rajcan, Istvan, Editor-in-Chief, Vollmann, Johann, Editor-in-Chief, and Van Huylenbroeck, Johan, editor

Published
2018
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37. Elucidating the triplicated ancestral genome structure of radish based on chromosome-level comparison with the Brassica genomes

Author

Jeong, Young-Min, Kim, Namshin, Ahn, Byung Ohg, Oh, Mijin, Chung, Won-Hyong, Chung, Hee, Jeong, Seongmun, Lim, Ki-Byung, Hwang, Yoon-Jung, Kim, Goon-Bo, Baek, Seunghoon, Choi, Sang-Bong, Hyung, Dae-Jin, Lee, Seung-Won, Sohn, Seong-Han, Kwon, Soo-Jin, Jin, Mina, Seol, Young-Joo, Chae, Won Byoung, Choi, Keun Jin, Park, Beom-Seok, Yu, Hee-Ju, and Mun, Jeong-Hwan

Published
2016
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49. Karyotype and nuclear DNA content analyses of Korean native Hibiscus‘Gangneung’ and ‘Baekryungdo’ using fluorescence in situ hybridization and flow cytometry

Author

Deepo, Deen Mohammad, Islam, Md Mazharul, Yesmin, Reshma, Lee, Hye-Min, Kim, Hong-Yul, Kim, Chang-Kil, and Lim, Ki-Byung

Abstract

Determination of nuclear DNA content, genome size, ploidy degree as well as prior knowledge on cytogenetic properties are prerequisites for modern plant breeding. The objectives of this study were to determine (1) 2C-DNA contents, genome sizes, and ploidy degree, (2) number of chromosomes, (3) 5S and 18S rDNA loci distribution, and 4) karyomorphology of two Korean native cultivars of Hibiscus‘Gangneung’ and ‘Baekryungdo’. According to the flow cytometry results, the 2C-DNA contents of both ‘Gangneung’ and ‘Baekryungdo’ were nearly identical, with 2.14 pg and 2.18 pg respectively. Additionally, putative DNA content were 2091.32 Mbp and 2135.00 Mbp for ‘Gangneung’ and ‘Baekryungdo’ respectively. In this study, we determined that H. syriacus‘Gangneung’ had 84 chromosomes, whereas ‘Baekryungdo’ had 90. We detected 5S rDNA and 18S rDNA loci through fluorescence in situ hybridization (FISH) technique. FISH results showed one pair of 5S rDNA loci in both cultivars, whereas two and three pairs of 18S rDNA loci were detected in ‘Gangneung’ and ‘Baekryungdo’, respectively. In ‘Gangneung,’ the 5S rDNA loci pair was detected in the short arm of chromosome #22, whereas in ‘Baekryungdo,’ the 5S loci was found in the centromere of chromosome #7. Furthermore, one 18S rDNA pair was found on the long arm of chromosome #14 and another pair on the short arm of chromosome #20 in ‘Gangneung’. Two pairs were found in the long arms of chromosome #11 and #20 for ‘Baekryungdo,’ while the last pair was found in the short arm of chromosome #45. The somatic chromosome’ length in ‘Gangneung’ and ‘Baekryungdo’ ranged from 2.10 to 7.31 μm and from 2.21 to 6.85 μm, respectively. To the best of our knowledge, this study is the first cytogenetic report of Korea native ‘Gangneung’ and ‘Baekryungdo’ cultivars. These findings will contribute to the detailed cytogenetic assessment of both the ‘Gangneung’ and ‘Baekryungdo’ cultivars and benefit plant breeding within this genus.

Published
2023
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