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Your search keyword '"Luneva, Oksana G."' showing total 5 results
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5 results on '"Luneva, Oksana G."'

3. Hemolysis and ATP release from human and rat erythrocytes under conditions of hypoxia: a comparative study

Author

Luneva, Oksana G., Novozhilova, T. S., Alekseeva, Natalya V., Rodnenkov, Oleg V., Deev, Leonid I., Maksimov, Georgy V., Grygorczyk, Ryszard, Orlov, Sergei N., and Sidorenko, Svetlana V.

Subjects
эритроциты, аденозинтрифосфорная кислота, гемолиз, молекулярные механизмы, экто-АТФаза
Abstract

Red blood cells are involved not only in transportation of oxygen and carbon dioxide but also in autoregulation of vascular tone by ATP release in hypoxic conditions. Molecular mechanisms of the ATP release from red blood cells in response to a decrease in partial oxygen pressure still remain to be elucidated. In this work we have studied effects of hypoxia on red blood cell hemolysis in humans and rats and compared the effects of inhibitors of ecto-ATPase and pannexin on the release of ATP and hemoglobin from rat erythrocytes. The 20-min hypoxia at 37°C increased hemolysis of red blood cells in humans and rats 1.5- and 2.5-fold, respectively. In rat erythrocytes a significant increase in hypoxia-induced extracellular ATP level was found only in the presence of ecto-ATPase inhibitor ARL 67156. In these conditions we observed a positive correlation (R2 = 0.5003) between the increase in free hemoglobin concentration and the ATP release. Neither carbenoxolon nor probenecid, the inhibitors of low-selectivity pannexin channels, altered the hypoxia-induced ATP release from rat erythrocytes. The obtained results indicate a key role of hemolysis in the ATP release from red blood cells.

Published
2018

4. Deoxygenation Affects Composition of Membrane-Bound Proteins in Human Erythrocytes

Author

Luneva, Oksana G., primary, Sidorenko, Svetlana V., additional, Ponomarchuk, Olga O., additional, Tverskoy, Artem M., additional, Cherkashin, Aleksander A., additional, Rodnenkov, Oleg V., additional, Alekseeva, Natalia V., additional, Deev, Leonid I., additional, Maksimov, Georgy V., additional, Grygorczyk, Ryszard, additional, and Orlov, Sergei N., additional

Published
2016
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5. Effect of the insecticide clothianidin on the photosynthetic electron transport chain in pea.

Author

Volgusheva, Alena A., Hao, Jingrao, He, Yanlin, Lovyagina, Elena R., Loktyushkin, Aleksey V., Parshina, Evgenia Yu, Luneva, Oksana G., Baizhumanov, Adil A., Khruschev, Sergei S., Maksimov, Georgy V., and Rubin, Andrew B.

Subjects
*CLOTHIANIDIN, *NEONICOTINOIDS, *ELECTRON transport, *INSECTICIDES, *BINDING sites, *CHLOROPLAST membranes, *PHOTOSYSTEMS
Abstract

Clothianidin (CL) is a neonicotinoid insecticide widely used in crop protection against insect pests. However, its effects on photosynthesis remain largely unknown. Here, by investigating the influence of CL at the concentrations of 22 and 110 μg/L on the primary processes of photosynthesis, membrane fluidity and structural changes of pea chloroplasts, we located several primary binding sites of this pesticide. Similar dynamics were observed for both concentrations. However, statistically significant differences were only found at 110 μg/L for all methods used. The light saturated rate of linear electron flow decreased mainly due to the disturbance of electron flow on the acceptor side of photosystem II (PSII) associated with the appearance of QB‐nonreducing centers and empty QB binding sites of PSII. The functioning of the donor side of PSII, the activity of photosystem I (PSI) and the maximum quantum yield of PSII photochemistry (Fv/Fm) were not found to be significantly altered. Increased membrane fluidity and structural alterations of the thylakoid membrane led to a decrease in the development of the proton gradient ΔрН and membrane energization processes. [ABSTRACT FROM AUTHOR]

Published
2024
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