1. HlyF Produced by Extraintestinal Pathogenic Escherichia coli Is a Virulence Factor That Regulates Outer Membrane Vesicle Biogenesis
- Author
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Emmanuelle Helloin, Sébastien Houle, Tetsuya Hayashi, Marie Penary, Patricia Martin, Gaëlle Porcheron, Kazunori Murase, Charles M. Dozois, Eric Oswald, Jean-Philippe Nougayrède, University of Miyazaki, Université Fédérale Toulouse Midi-Pyrénées, Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Laboratoire de Comportement, INRA-CNRS, 37380 Nouzilly, France, Institut National de la Recherche Agronomique (INRA), Department of Applied Chemistry, Osaka University [Osaka], CHU Toulouse [Toulouse], This work was supported by the French government, through the Investments for the Future program (ANR-11-EQPX-0003), the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Grants-in-Aid for Young [B] 26860293 to K. M.), the National Science and Engineering Research Council of Canada (Discovery grant to C. M. D.), and a Canada Research Chair (to C. M. D.)., ANR-11-EQPX-0003/11-EQPX-0003,ANINFIMIP,Equipements plateforme animalerie infectieuse de haute-sécurité de Midi Pyrénées(2011), ANR-11-EQPX-0003,ANINFIMIP,Equipements plateforme animalerie infectieuse de haute-sécurité de Midi Pyrénées(2011), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Courcelles, Michel, Equipements plateforme animalerie infectieuse de haute-sécurité de Midi Pyrénées - - ANINFIMIP2011 - ANR-11-EQPX-0003 - EQPX - VALID, Institut de Recherche en Santé Digestive (IRSD ), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
0301 basic medicine ,[SDV]Life Sciences [q-bio] ,MESH: Escherichia coli Proteins ,Vacuole ,MESH: Virulence ,medicine.disease_cause ,Virulence factor ,Immunology and Allergy ,MESH: Animals ,MESH: Phylogeny ,Escherichia coli Infections ,Phylogeny ,Extraintestinal Pathogenic Escherichia coli ,MESH: Gene Expression Regulation, Bacterial ,biology ,Virulence ,MESH: Escherichia coli ,Escherichia coli Proteins ,pathogenesis ,MESH: Chickens ,OMV ,3. Good health ,[SDV] Life Sciences [q-bio] ,MESH: Mutagenesis, Site-Directed ,Infectious Diseases ,Bacterial outer membrane ,Hemolysin Factors ,Virulence Factors ,MESH: Poultry Diseases ,MESH: Vacuoles ,Microbiology ,03 medical and health sciences ,medicine ,Autophagy ,Escherichia coli ,Animals ,Humans ,MESH: Autophagy ,Poultry Diseases ,MESH: Virulence Factors ,MESH: Escherichia coli Infections ,MESH: Humans ,Cell Membrane ,Pathogenic bacteria ,Gene Expression Regulation, Bacterial ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,HlyF ,030104 developmental biology ,Vacuoles ,MESH: Hemolysin Factors ,Mutagenesis, Site-Directed ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Chickens ,Bacteria ,MESH: Cell Membrane - Abstract
International audience; Escherichia coli can cause extraintestinal infections in humans and animals. The hlyF gene is epidemiologically associated with virulent strains of avian pathogenic E. coli and human neonatal meningitis-associated E. coli. We demonstrated that culture supernatants of E. coli expressing HlyF induced autophagy in eukaryotic cells. This phenotype coincided with an enhanced production of outer membrane vesicles (OMVs) by bacteria expressing HlyF. The HlyF protein displays a predicted catalytic domain of the short-chain dehydrogenase/reductase superfamily. This conserved domain was involved the ability of HlyF to promote the production of OMVs. The increased production of OMVs was associated with the release of toxins. hlyF was shown to be expressed during extraintestinal infection and to play a role in the virulence of extraintestinal pathogenic E. coli in a chicken model of colibacillosis. This is the first evidence that pathogenic bacteria produce a virulence factor directly involved in the production of OMVs.
- Published
- 2016