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Your search keyword '"Makhotkin, M. A."' showing total 11 results
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11 results on '"Makhotkin, M. A."'

11. [The miRNA aberrant expression dependence on DNA methylation in HeLa cells treated with mitomycin C].

Author

Tarasov VA, Boyko NV, Makhotkin MA, Shin EF, Tyutyakina MG, Chikunov IE, Naboka AV, Mashkarina AN, Kirpiy AA, and Matishov DG

Subjects
HeLa Cells, Humans, DNA Methylation drug effects, DNA Methylation genetics, Gene Expression Regulation drug effects, MicroRNAs biosynthesis, MicroRNAs genetics, Mitomycin pharmacology
Abstract

The dependence of expression of miRNAs and their precursors (pre-miRNAs) on the DNA methylation level in HeLa cells 8 days after mitomycin C treatment was studied. A massive parallel DNA sequencing method was applied to analyze miRNA expression. 5-Azacytidine (DNA methylation inhibitor) was added to the medium 6 days after mutagenic agent exposure. The results indicated that the change in expression for some mature miRNAs (39 of 61) was accompanied by the change in the expression of their pre-miRNAs, while there were no significant changes in the expression of pre-miRNA for other mature miRNAs (22 of 61). The aberrant expression was maintained by 8 of 61 mature miRNAs and 6 of 55 pre-miRNAs in the induced HeLa cells after 5-azacytidine treatment. In addition, the expression of more than 90% of miRNAs, which indicated a significant change in expression after mitomycin C treatment, does not depend or depends slightly on the DNA methylation level in HeLa cells without mitomycin C treatment. The results suggest that mitomycin C induces aberrant DNA methylation which affects maintenance of changes in the miRNA expression in cell generations after mutagen treatment.

Published
2016

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