25 results on '"Meenakshi Bhardwaj"'
Search Results
2. Cytosolic glucosylceramide regulates endolysosomal function in Niemann-Pick type C disease
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Simon Wheeler, Per Haberkant, Meenakshi Bhardwaj, Paige Tongue, Maria J. Ferraz, David Halter, Hein Sprong, Ralf Schmid, Johannes M.F.G. Aerts, Nikol Sullo, and Dan J. Sillence
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Sphingolipid ,Glycolipids ,Endocytosis ,Neurodegenerative disease ,Lysosomal acidification ,GBA2 ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Niemann-Pick type C disease (NPCD) is a neurodegenerative disease associated with increases in cellular cholesterol and glycolipids and most commonly caused by defective NPC1, a late endosomal protein. Using ratiometric probes we find that NPCD cells show increased endolysosomal pH. In addition U18666A, an inhibitor of NPC1, was found to increase endolysosomal pH, and the number, size and heterogeneity of endolysosomal vesicles. NPCD fibroblasts and cells treated with U18666A also show disrupted targeting of fluorescent lipid BODIPY-LacCer to high pH vesicles. Inhibiting non-lysosomal glucocerebrosidase (GBA2) reversed increases in endolysosomal pH and restored disrupted BODIPY-LacCer trafficking in NPCD fibroblasts. GBA2 KO cells also show decreased endolysosomal pH. NPCD fibroblasts also show increased expression of a key subunit of the lysosomal proton pump vATPase on GBA2 inhibition. The results are consistent with a model where both endolysosomal pH and Golgi targeting of BODIPY-LacCer are dependent on adequate levels of cytosolic-facing GlcCer, which are reduced in NPC disease. more...
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- 2019
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Catalog
3. Epithelioid Hemangioendothelioma of Tibia andTalus: A Case Report
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Dharmendra Kumar, Vijay K. Jain, Meenakshi Bhardwaj, Ananta K. Naik, Ruchi Nasa, and Rajendra K. Arya
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Hemangioendothelioma Epithelioid ,Bone and Bones ,Talus ,Tibia ,Medicine - Abstract
Epithelioid hemangioendothelioma (EHE) of the bone is extremely uncommon and consists of less than 1% of primary bone tumors. It is characterized by epithelioid endothelial cells and has variable biological behavior. EHE is more likely to occur between 20 and 30 years of age. Approximately half of EHE present with multifocal disease. Since the behavior of these tumors is intermediate, it is important to not misdiagnose EHE as an angiosarcoma. Here we describe the case of a 43-year-old male who presented with pain and swelling of the leg and ankle to Dr. Ram Manohar Lohia Hospital, India. Radiography and computed tomography of the ankle and leg were performed and suggested an expansile lytic lesion involving the distal tibia and talus bone. The lesion was excised and ankle arthrodesis performed. Histological evaluation of the lesion demonstrated the presence of EHE of the tibia and talus. more...
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- 2015
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4. Forensic Statement Analysis to determine Personality Traits-A Case Study
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-, Meenakshi Bhardwaj, primary and -, Asha Srivastava, additional
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- 2023
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5. Methyldecanoate isolated from marine algae Turbinaria ornata enhances immunomodulation in LPS-induced inflammatory reactions in RAW 264.7 macrophages via iNOS/NFκB pathway
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Meenakshi Bhardwaj, Veeresh Kumar Sali, R. Malarvizhi, Sugumar Mani, T. K. Padmavathy, and Hannah Rachel Vasanthi
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Pharmacology ,Immunology ,Pharmacology (medical) - Abstract
This study identifies the anti-inflammatory, antioxidant, and immunomodulatory potential of a fatty acid methyl ester segregated from the brown algae Turbinaria ornata and identified by nuclear magnetic resonance and mass spectrometry as methyl 6,12-dimethyltridecanoate (ET). Antioxidant and anti-inflammatory effects of ET were studied on lipopolysaccharide (LPS)-induced inflammatory reaction in RAW 264.7 macrophages. Moreover, in silico docking studies of isolated ET with inflammatory markers TNFα, NFκB, and COX-2 showed potent binding scores suggesting anti-inflammatory potential. ET significantly reduced LPO and increased LPS-induced SOD, catalase, and GSH levels. Molecular docking results were further confirmed by checking mRNA levels of selected cytokines (IL6 and IL10), followed by protein expression of iNOS and NFκB in LPS-induced macrophages. ET significantly upregulated the expression of IL10 and downregulated the expression of IL6, iNOS, and NFκB, confirming the inhibition of LPS-induced inflammation via the iNOS/NFκB pathway. more...
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- 2022
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6. Cost-effectiveness of the prevention of parent-to-child transmission guidelines of HIV in India
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Seema Patrikar, Meenakshi Bhardwaj, Puja Dudeja, and Renuka Kunte
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General Medicine - Published
- 2022
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7. Methyldecanoate isolated from marine algae Turbinaria ornata enhances immunomodulation in LPS induced inflammation in RAW 264.7 macrophages via iNOS/NFκB pathway
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Meenakshi Bhardwaj, Veeresh Kumar Sali, R. Malarvizhi, Sugumar Mani, Padmavathy TK, and Hannah R Vasanthi
- Abstract
This study identifies the anti-inflammatory, antioxidant and immunomodulatory potential of a fatty acid methyl ester segregated from the brown algae Turbinaria ornata and identified by nuclear magnetic resonance and mass spectrometry as methyl6,12-dimethyltridecanoate (ET). Antioxidant and anti-inflammatory effects of ET were studied on lipopolysaccharide (LPS) induced inflammation in RAW 264.7 macrophages. Moreover, in silico docking studies of isolated ET with inflammatory markers TNFα, NFκB and COX-2 showed potent binding scores suggesting good anti-inflammatory potential. ET significantly reduced LPO and increased LPS induced SOD, catalase, and GSH levels. Molecular docking results were further confirmed by checking mRNA levels of common inflammatory/anti-inflammatory mediators IL6 and IL10, followed by protein expression of iNOS and NFκB in LPS induced macrophages. ET significantly upregulated the expression of IL10 and downregulated the expression IL6, iNOS, and NFκB confirming inhibition of LPS induced inflammation via the iNOS/NFκB pathway. more...
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- 2022
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8. Inhibition of Cyclooxygenase Enzyme by Bioflavonoids in Horsegram Seeds Alleviates Pain and Inflammation
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Sugumar Mani, Meenakshi Bhardwaj, Malarvizhi Ramalingam, Veeresh Kumar Sali, and Hannah R. Vasanthi
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Male ,Analgesic ,Anti-Inflammatory Agents ,Pain ,Pharmacology ,Catechin ,Rats, Sprague-Dawley ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Diclofenac ,Drug Discovery ,medicine ,Animals ,Humans ,030304 developmental biology ,Flavonoids ,Inflammation ,chemistry.chemical_classification ,Analgesics ,0303 health sciences ,Cyclooxygenase 2 Inhibitors ,Dose-Response Relationship, Drug ,biology ,Plant Extracts ,Chemistry ,Anti-Inflammatory Agents, Non-Steroidal ,Organic Chemistry ,Daidzein ,food and beverages ,Fabaceae ,General Medicine ,Isoflavones ,In vitro ,Computer Science Applications ,Molecular Docking Simulation ,Enzyme ,Prostaglandin-Endoperoxide Synthases ,Docking (molecular) ,030220 oncology & carcinogenesis ,Seeds ,biology.protein ,COX-2 inhibitor ,Cyclooxygenase ,Protein Binding ,medicine.drug - Abstract
Background: Inflammation and pain, mainly induced by the prostaglandins synthesized by the cyclooxygenase enzymes, may cause distress. To overcome this unpleasant stress in a safer manner, numerous natural molecules are proven for modulating the COX enzymes. Epicatechin and daidzein are two bioactive natural compounds present in horsegram, a legume known for its medicinal properties. Objective: The present study aims at evaluating the potential of horsegram, and some of its bioactive molecules, to be used as an anti-inflammatory and analgesic agent mediated by the inhibition of COX enzymes, which can be recommended as a substitute for chemically synthesized NSAIDs. Methods: The present work involved the quantification of epicatechin and daidzein present in horsegram seeds. The COX enzyme inhibitory nature of epicatechin and daidzein was tested using in silico docking analysis with Autodock software and was further confirmed by in vitro COX inhibitory biochemical assays. Furthermore, the anti-inflammatory and analgesic activities of the horsegram seeds were evaluated in animal experiments. Results: Horsegram seeds contain 158.1 microgram/g and 6.51 microgram/g of epicatechin and daidzein respectively. The docking studies reveal that both the bioactive molecules exhibit better binding efficiency with COX-2 protein as compared to COX-1. Hence, in vitro COX-2 inhibitory assay was performed for epicatechin, daidzein and compared with known analgesic agent diclofenac which revealed a pronounced dose dependent inhibitory activity. Furthermore, the analgesic and anti-inflammatory activity of horsegram in experimental animals exhibited a dose dependent effect which might be due to the presence of the bioactive compounds such as epicatechin and daidzein. Conclusion: The results suggest that epicatechin and daidzein present in horsegram are potent cyclooxygenase inhibitors and thus would be helpful in the management of inflammation and pain. more...
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- 2020
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9. Pleural mass in a young male unfolding an uncommon presentation of multiple myeloma
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Meenakshi Bhardwaj, Arvind Ahuja, Purnima Bharati, and Prajwala Gupta
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medicine.medical_specialty ,business.industry ,pleural mass ,nonsecretory ,Pleural Mass ,medicine.disease ,Dermatology ,multiple myeloma ,fnac ,immunocytochemistry ,hemic and lymphatic diseases ,lcsh:Pathology ,Medicine ,Presentation (obstetrics) ,business ,Multiple myeloma ,Young male ,lcsh:RB1-214 - Abstract
Multiple myeloma is a malignant proliferation of the plasma cells and can be non-secretory in about 1 to 5 % cases. Pleura based mass lesions are uncommon in myeloma. Herein we report a case of non-secretory multiple myeloma presenting with a pleura based mass in a 40 year old male. Fine needle aspiration cytology with immunohistochemistry played a key role in the diagnosis of this case. more...
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- 2020
10. Refinement of LPS induced Sepsis in SD Rats to Mimic Human Sepsis
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Rutuja Patil, Meenakshi Bhardwaj, A Hannah Rachel Vasanthi, Sugumar Mani, and R. Malarvizhi
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Pharmacology ,Sepsis ,business.industry ,Medicine ,business ,medicine.disease - Published
- 2020
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11. Diosgenin Promoted Glucose Uptake by Attenuation of High Glucose Induced Blockade of PI3K-Akt-GLUT4 Pathway and ER Stress
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Ramesh Prajapath, Veeresh Kumar Sali, Meenakshi Bhardwaj, R. Malarvizhi, and Hannah Rachel Vasanthi
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- 2022
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12. Biomedical Potential of Marine Macroalgae in Modulating Chronic Disease Pathologies
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Meenakshi Bhardwaj and Hannah R. Vasanthi
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Chronic disease ,business.industry ,Immunology ,Medicine ,business - Published
- 2021
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13. Neophytadiene from Turbinaria ornata Suppresses LPS-Induced Inflammatory Response in RAW 264.7 Macrophages and Sprague Dawley Rats
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Veeresh Kumar Sali, Meenakshi Bhardwaj, Hannah R. Vasanthi, and Sugumar Mani
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Lipopolysaccharides ,Male ,0301 basic medicine ,Necrosis ,Lipopolysaccharide ,Immunology ,Anti-Inflammatory Agents ,Inflammation ,Pharmacology ,Phaeophyta ,Nitric oxide ,Proinflammatory cytokine ,Rats, Sprague-Dawley ,Biological Factors ,Mice ,Random Allocation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Immunology and Allergy ,Dose-Response Relationship, Drug ,biology ,Macrophages ,Interleukin ,Rats ,Nitric oxide synthase ,RAW 264.7 Cells ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,TLR4 ,Inflammation Mediators ,medicine.symptom - Abstract
This study investigates the mode of action of Neophytadiene (MT), a molecule isolated from a marine algae Turbinaria ornata in LPS-induced inflammation in both in vitro and in vivo conditions. Neophytadiene (25, 50, 100 μM/mL) was treated to LPS-stimulated RAW 264.7 macrophages cells to identify its anti-inflammatory potential by measuring the level of tumour necrosis factor (TNF-α) by enzyme-linked immunosorbent assay (ELISA) and nitric oxide (NO) using Griess reagent. The mRNA levels of inflammatory cytokines, interleukin (IL-6 and IL-10), and the protein expression of nuclear factor-κB (NF-κB) and inducible nitric oxide synthase (iNOS) were quantified by Western blot analysis. Subsequently, Neophytadiene (12, 25, 50 mg/kg b.wt/p.o) was pre-treated for 7 days to the experimental animals followed by LPS (10 mg/kg) injection interaperitonially. After LPS induction, blood was collected and the haematological parameters were analysed followed by isolation of heart tissue for biochemical molecular and histopathological analysis Neophytadiene significantly inhibited the NO production and inflammatory cytokines TNF-α, IL-6 and IL-10 both in in vitro and in vivo conditions. Further, the expression of TNF-α, IL1β, NF-κB, iNOS, PI3k/Akt and MAPK in the heart tissue was modulated by Neophytadiene significantly confirming the anti-inflammatory potential. Thus, the effect of Neophytadiene on LPS-induced cardiac injury can be attributed to its anti-inflammatory antioxidant and cardioprotective properties. more...
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- 2020
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14. Effect of con-joint application of inorganic and organic sources of nutrients with biofertilizers on growth, yield and fruit quality of Kinnow mandarin
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Vipan Guleria, Dharminder Kumar, Meenakshi Bhardwaj, Rajesh Kumar, Vgs Chandel, and Atul Gupta
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Horticulture ,Nutrient ,Biofertilizer ,Yield (finance) ,media_common.quotation_subject ,language ,Quality (business) ,Mandarin Chinese ,Joint (geology) ,language.human_language ,Mathematics ,media_common - Published
- 2020
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15. Macrotyloma unifloruma plant food alleviates the metabolic syndrome through modulation of adipokines and PPARs
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Hannah R. Vasanthi, R. Malarvizhi, Sugumar Mani, Meenakshi Bhardwaj, and Veeresh Kumar Sali
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030309 nutrition & dietetics ,Biophysics ,Adipokine ,03 medical and health sciences ,0404 agricultural biotechnology ,Nutraceutical ,Adipokines ,Diabetes mellitus ,Hyperlipidemia ,Animals ,Medicine ,Obesity ,Metabolic Syndrome ,Pharmacology ,0303 health sciences ,Adiponectin ,Traditional medicine ,business.industry ,Fabaceae ,04 agricultural and veterinary sciences ,Cell Biology ,medicine.disease ,040401 food science ,Rats ,Human nutrition ,Dietary Supplements ,Metabolic syndrome ,business ,Food Science - Abstract
A sedentary lifestyle combined with the intake of high-calorie diet has been the paramount cause of metabolic syndrome (MS) which is now a serious concern of public health worldwide as it involves the coexistence of hypertension, hyperlipidemia, glucose intolerance, and obesity. Hence, identifying a suitable strategy to overcome the worldwide menace of MS is imperative. Macrotyloma uniflorum a lesser known legume is highly nutritious and notable for its ethano-medicinal potential. Herein, the influence of M. uniflorum in high-fat dietinduced metabolic changes in a rodent model of metabolic syndrome was evaluated. Serum levels of glucose, total cholesterol, triglycerides, VLDL-c, and bodyweight were decreased, whereas HDL-c was increased in M. uniflorum-treated MS rats. The protein expression (AMPK-α, PPAR-α, and PPAR-γ) and gene expression (leptin, adiponectin, resistin, UCP2, NF-κB, and IL-6) results are impressive to highlight that M. uniflorum modulates the pathological conditions of MS and proves to be cardioprotective. Furthermore, the histopathological analysis confirmed the pathological changes and substantiates the influence of M. uniflorum to overcome MS. The HPLC and GC (MS) profiling reveals the presence of an array of polyphenols such as rutin (694.61 μg/g), catechin (500.12 μg/g), epicatechin (158.10 μg/g), gallic acid (17.98 μg/g), ferulic acid (10.911 μg/g), daidzein (6.51 μg/g), and PUFA, respectively, which probably exhibits the therapeutic effect on MS and associated complications by modulating lipid metabolism and adipogenesis. PRACTICAL APPLICATIONS: Metabolic disorders like CVD and diabetes are leading cause of mortality and morbidity worldwide. With emerging issues on adverse effects of modern drugs, the emphasis on "Food is Medicine and Medicine as Food" has taken dramatic dimensions in the healthcare sector. Therefore, nutraceuticals are in great demand in the developed world off late. Legumes, are potent elements in a balanced diet next to cereals. Exploring the medicinal properties of legumes could bring a revolution in public health and nutraceutical industries. This study scientifically validated the phytochemicals in M. uniflorum for its functional potential in the management of Metabolic Syndrome (MS). This study would help the nutraceutical industries to develop functional foods using M. uniflorum seeds to make porridges and soups or nutraceutical supplements with the bioflavonoids isolated from M. uniflorum for the management of metabolic disorders by mitigating hyperlipidemia, oxidative stress, and inflammation. more...
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- 2020
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16. Sulfated polysaccharide from Turbinaria ornata suppress lipopolysaccharide-induced inflammatory response in RAW 264.7 macrophages
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R. Malarvizhi, Sugumar Mani, Hannah R. Vasanthi, Veeresh Kumar Sali, T K Padmavathy, and Meenakshi Bhardwaj
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Lipopolysaccharides ,Antioxidant ,Lipopolysaccharide ,Cell Survival ,medicine.medical_treatment ,Anti-Inflammatory Agents ,Inflammation ,02 engineering and technology ,medicine.disease_cause ,Polysaccharide ,Phaeophyta ,Biochemistry ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Structural Biology ,Polysaccharides ,Turbinaria ornata ,medicine ,Animals ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,biology ,Macrophages ,General Medicine ,Glutathione ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Oxidative Stress ,RAW 264.7 Cells ,chemistry ,Tumor necrosis factor alpha ,medicine.symptom ,0210 nano-technology ,Oxidative stress ,Biomarkers - Abstract
Background Marine macroalgae known for its polysaccharides exhibit potent biomedical properties and its potential as an anti-inflammatory agent has increased in the recent past as inflammation is a major pathology noted in many chronic diseases. Purpose The present study investigates the anti-inflammatory potential of a sulfated polysaccharide (PS) isolated from the marine algae Turbinaria ornata collected from the Indian waters on LPS induced inflammation in RAW 264.7 macrophages. Study design and methods PS isolated from the macroalgae was characterized using ESI(MS) and was screened for its antioxidant and anti-inflammatory potential in RAW 264.7 cells by assessing markers of oxidative stress, and inflammation. Results LPS significantly increased the levels of LPO and LDH in RAW 264.7 cells which were significantly reduced in PS pre-treatment groups. Pretreatment significantly increased the antioxidants GSH and SOD and significantly reduced mRNA levels of IL6 and TNFα in vitro confirming its anti-inflammatory potential. NFκB and iNOS were significantly modulated by PS confirming the probable mode of action. Conclusion Altogether, it can be concluded that PS isolated from Turbinaria ornata collected from the Southeast Coast of India exhibits antioxidant and anti-inflammatory potential probably mediated by the sulfated polysaccharide containing glucopyranose and fucopyranose moieties. more...
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- 2020
17. Correction to: Neophytadiene from Turbinaria ornata Suppresses LPS-Induced Inflammatory Response in RAW 264.7 Macrophages and Sprague Dawley Rats
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Meenakshi Bhardwaj, Veeresh Kumar Sali, Sugumar Mani, and Hannah R. Vasanthi
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Immunology ,Immunology and Allergy - Abstract
The publisher made a mistake in the published version of this article.
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- 2020
18. Retraction notice to 'Attenuation of inflammation by marine algae Turbinaria ornata in cotton pellet induced granuloma mediated by fucoidan like sulphated polysaccharide' [Carbohydr. Polym. 151 (2016) 1261–1268]
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Ananthi Subash, Meenakshi Bhardwaj, Veeresh Kumar Sali, Gayathri Veeraraghavan, and Hannah R. Vasanthi
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Cotton pellet ,Polymers and Plastics ,biology ,Fucoidan ,Organic Chemistry ,Inflammation ,biology.organism_classification ,medicine.disease ,Microbiology ,chemistry.chemical_compound ,chemistry ,Algae ,Granuloma ,Sulphated polysaccharides ,Turbinaria ornata ,Materials Chemistry ,medicine ,medicine.symptom - Published
- 2021
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19. Immunomodulatory activity of brown algae Turbinaria ornata derived sulfated polysaccharide on LPS induced systemic inflammation
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Sugumar Mani, Hannah R. Vasanthi, Veeresh Kumar Sali, Meenakshi Bhardwaj, and R. Malarvizhi
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Lipopolysaccharides ,Male ,Antioxidant ,Lipopolysaccharide ,medicine.medical_treatment ,Pharmaceutical Science ,Inflammation ,Pharmacology ,Phaeophyta ,Systemic inflammation ,medicine.disease_cause ,Immunomodulation ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Polysaccharides ,Drug Discovery ,Turbinaria ornata ,medicine ,Animals ,030304 developmental biology ,0303 health sciences ,biology ,Sulfates ,Glutathione ,biology.organism_classification ,Rats ,Oxidative Stress ,Interleukin 10 ,Complementary and alternative medicine ,chemistry ,030220 oncology & carcinogenesis ,Molecular Medicine ,medicine.symptom ,Oxidative stress - Abstract
Background Inflammation and oxidative stress are common pathologies in a wide range of chronic diseases. Polysaccharides are known to exhibit antioxidant and anti-inflammatory potential and are suggested to possess immunomodulatory potential. Purpose Herein, the immunomodulatory activity of a sulfated polysaccharide (PS) separated from a brown marine algae Turbinaria ornata is studied in LPS instigated systemic inflammation in experimental rats. Study design and methods Male SD rats are pretreated with different doses of PS (2.5, 5, 10 mg/kg bw) for a week followed by inducing systemic inflammation using LPS (10 mg/kg i.p.). Blood withdrawn after 8 h of LPS injection is subjected to hematological analysis (WBC, HCT, and PLT). After 24 h of LPS induction, cardiac tissue was isolated and subjected to biochemical, molecular, and histopathological analysis. Effect of PS pre-treatment (2.5, 5, 10 mg/kg bw) was checked by assessing serum parameters (AST, CK-MB, and γGT), antioxidant markers (LPO, GSH, SOD, Grx) and inflammatory markers (IL1β, IL6, IL10, NFκB), followed by analyzing the iNOS, PI3k and Akt to identify the probable mode of action. Results Elevated levels of AST, CK-MB, and γGT in serum were significantly reduced on PS pretreatment. LPS significantly raised the LPO and Grx levels in heart tissue whereas, PS pre-treatment significantly reduced LPO and Grx levels. GSH and SOD levels were reduced upon LPS induction and were brought to near normal by HD of PS. PS also reduced the mRNA levels of IL6, Trx, and increased IL10 levels in the heart tissue substantiating its anti-inflammatory and antioxidant potency. Further, IL1β, NFκB, iNOS, and pPI3k/pAkt expressions were significantly modulated by PS in the cardiac tissue substantiating the immunomodulatory effect. A trend of improvement in the inflammatory pathology was also observed in the heart tissue compared to LPS control, as confirmed by histopathology analysis. Conclusion Altogether, this study concludes the immunomodulatory potential of PS from the marine macroalgae Turbinaria ornata significantly and prevents LPS induced systemic inflammation in the cardiac tissue presumably influenced by the glucopyranose and fucopyranose subunits in the polysaccharide. more...
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- 2021
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20. Anti-arthritic potential of marine macroalgae Turbinaria ornata in Complete Freund’s Adjuvant induced rats
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Hannah R. Vasanthi, R. Malarvizhi, S. Ananthi, Meenakshi Bhardwaj, and V. Gayathri
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0301 basic medicine ,Antioxidant ,medicine.medical_treatment ,Freund's Adjuvant ,Anti-Inflammatory Agents ,Arthritis ,Inflammation ,Pharmacology ,Phaeophyta ,Toxicology ,medicine.disease_cause ,Antioxidants ,Pathology and Forensic Medicine ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Polysaccharides ,Turbinaria ornata ,medicine ,Animals ,Dexamethasone ,030203 arthritis & rheumatology ,biology ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Chemistry ,Cell Biology ,General Medicine ,medicine.disease ,biology.organism_classification ,Arthritis, Experimental ,Oxidative Stress ,C-Reactive Protein ,030104 developmental biology ,Freund's adjuvant ,Immunology ,medicine.symptom ,Adjuvant ,Biomarkers ,Oxidative stress ,medicine.drug - Abstract
T. ornata a macroalgae rich in bioactive molecules possess various biological activities. Herein, the aim of the study is to evaluate the aqueous extract and the sulphated polysaccharide isolated from T. ornata for its anti-arthritic potential in Complete Freund's Adjuvant (CFA) induced arthritis in rats. Anti-arthritic potential of aqueous T. ornata (ATO) and T. ornata sulphated polysaccharide (TSP) was evidenced by the significant reduction in paw volume and arthritic score. Inflammatory and antioxidant markers were found to be restored in the drug treated groups which was found to be in line with dexamethasone a standard anti-inflammatory drug. The histopathological and radiological examination adds on the support to the above findings confirming the anti-arthritic potential of ATO and TSP. It is interesting to note that the sulphated polysaccharide inhibits inflammation and bone damage at very low dose itself. Hence, TSP could be considered as a better candidate in the management of chronic inflammatory diseases like rheumatoid arthritis. more...
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- 2017
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21. Cytosolic glucosylceramide regulates endolysosomal function in Niemann-Pick type C disease
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David Halter, Johannes M. F. G. Aerts, Daniel J. Sillence, Hein Sprong, Maria J. Ferraz, Meenakshi Bhardwaj, Per Haberkant, Simon Wheeler, Ralf Schmid, Paige Tongue, and Nikol Sullo
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0301 basic medicine ,Endosome ,GBA2 ,Endosomes ,Glucosylceramides ,Endocytosis ,Neurodegenerative disease ,Sphingolipid ,lcsh:RC321-571 ,Mice ,03 medical and health sciences ,Cytosol ,0302 clinical medicine ,Glycolipid ,Niemann-Pick C1 Protein ,Animals ,Humans ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Chemistry ,Vesicle ,Lysosomal acidification ,nutritional and metabolic diseases ,Niemann-Pick Disease, Type C ,Golgi Targeting ,Fibroblasts ,Cell biology ,030104 developmental biology ,Neurology ,Androstenes ,NPC1 ,Glycolipids ,Lysosomes ,030217 neurology & neurosurgery - Abstract
A new paradigm for Niemann-Pick C disease is presented where lysosomal storage leads to a deficit in cytoplasmic glucosylceramide (GlcCer) where it performs important functions. Previously it had been reported that Gaucher cells have defective endolysosomal pH. GlcCer also accumulates in Niemann-Pick C disease and also shows this defect. Niemann-Pick C cells were found to have reduced cytoplasmic glucosylceramide (GlcCer) transport. Inhibiting cytoplasmic glucocerebrosidase (GBA2), increased GlcCer, decreased endolysosomal pH in normal cells, reversed increases in endolysosomal pH and restored disrupted BODIPY-LacCer trafficking and increased expression of vATPase a subunit in Niemann-Pick C fibroblasts. The results are consistent with a model where both endolysosomal pH and Golgi targeting of BODIPY-LacCer are dependent on adequate levels of cytosolic GlcCer which are reduced in NPC disease. This work consequently suggests GBA2 and vATPase as new therapeutic targets in Niemann-Pick C and related neurodegenerative diseases. The work was in collaboration with colleagues in the Netherlands and Leicester University. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link. Niemann-Pick type C disease (NPCD) is a neurodegenerative disease associated with increases in cellular cholesterol and glycolipids and most commonly caused by defective NPC1, a late endosomal protein. Using ratiometric probes we find that NPCD cells show increased endolysosomal pH. In addition U18666A, an inhibitor of NPC1, was found to increase endolysosomal pH, and the number, size and heterogeneity of endolysosomal vesicles. NPCD fibroblasts and cells treated with U18666A also show disrupted targeting of fluorescent lipid BODIPY-LacCer to high pH vesicles. Inhibiting non-lysosomal glucocerebrosidase (GBA2) reversed increases in endolysosomal pH and restored disrupted BODIPY-LacCer trafficking in NPCD fibroblasts. GBA2 KO cells also show decreased endolysosomal pH. NPCD fibroblasts also show increased expression of a key subunit of the lysosomal proton pump vATPase on GBA2 inhibition. The results are consistent with a model where both endolysosomal pH and Golgi targeting of BODIPY-LacCer are dependent on adequate levels of cytosolic-facing GlcCer, which are reduced in NPC disease. more...
- Published
- 2019
22. Fibrosarcomatous dermatofibrosarcoma metastasizing to retroperitoneum four years after resection of primary lesion - A case report with review of literature
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Dipankar Naskar, Meenakshi Bhardwaj, Umesh Krishnegowda, Nikhil Gupta, Arun Gupta, and C. K. Durga
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medicine.medical_specialty ,Pathology ,Soft Tissue Neoplasm ,business.industry ,Exploratory laparotomy ,medicine.medical_treatment ,Soft tissue ,medicine.disease ,Malignancy ,Curvatures of the stomach ,medicine ,Dermatofibrosarcoma protuberans ,Histopathology ,business ,Dermatofibrosarcoma - Abstract
Dermatofibrosarcoma protuberans (DFSP) is a rare soft tissue neoplasm arising from mesenchymal cells, making up about 6% of all soft tissue tumours1. The most commonly involved sites are the trunk (42–72%) and proximal extremities (16–30%)2. It generally has intermediate- to low-grade malignancy potential with the exception of the fibrosarcomatous variant (high-grade). This variant is more likely to metastasise (in less than 5% of cases) to the lungs and lymph nodes and may occur many years after the onset of disease3. Herein, we present the case of a 40-yearold male who presented with a two-month history of a lump in the upper abdomen with pressure symptoms and past history of recurrent dermatofibrosarcoma of the left shoulder. Computed tomography (CT) revealed a mass arising from the greater curvature of the stomach. The patient underwent exploratory laparotomy which revealed a tumour mass arising from the retroperitoneum The mass was resected and sent for histopathology which revealed a fibrosarcomatous variant of dermatofibrosarcoma protuberans (FS-DFSP). more...
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- 2015
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23. Survey of major ethnomedicinal plants of District Kinnaur, Himachal Pradesh
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Meenakshi Bhardwaj, L. S. Kandari, and Tripti Negi
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Apiaceae ,biology ,Traditional medicine ,Desert climate ,fungi ,food and beverages ,04 agricultural and veterinary sciences ,010501 environmental sciences ,Asteraceae ,biology.organism_classification ,01 natural sciences ,Rhizome ,visual_art ,040103 agronomy & agriculture ,visual_art.visual_art_medium ,0401 agriculture, forestry, and fisheries ,Bark ,Medicinal plants ,0105 earth and related environmental sciences - Abstract
This paper highlights the medicinal plant wealth and its gradual depletion in Kinnaur district of Himachal Pradesh, India. In most cases, the entire plant is dug out for their rhizomes or roots thereby reducing the chances of regeneration. Out of the forty one plants species in the study areas, 83% belong to herbs, 7% to shrubs and 10% to trees. Apiaceae and Asteraceae being the predominant families represented by 7 species each. Maximum utilisation (56%) of roots and rhizomes was observed followed by whole plant (12.2%), seeds (10%), leaf and flower (7.3%), fruits (5%), barks and leaf (2.4%), and bark, stem and flower (2.4%). Out of 41 plants recorded in the present study, 10 were reported to cure stomachache followed by rheumatism and fever (09), and 4 by anaemia and skin disease. Thirty-three plants species have been reported to have more than one therapeutic uses, whereas 17 species are reported to be used for single ailment. more...
- Published
- 2020
- Full Text
- View/download PDF
24. Placenta accreta not previa: a rare case report of placenta accreta in an unscarred uterus
- Author
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Meenakshi Bhardwaj, Jaya Chawla, Renuka Malik, and Swati Singh
- Subjects
medicine.medical_specialty ,business.industry ,Placenta accreta ,Obstetrics ,embryonic structures ,Rare case ,Medicine ,business ,medicine.disease ,Unscarred uterus ,reproductive and urinary physiology - Abstract
Placenta accreta spectrum disorders are usually associated with direct surgical scar such as caesarean delivery, surgical termination of pregnancy, Dilatation and curettage, Myomectomy, Endometrial resection and Asherman’s syndrome. It can also be associated with non-surgical scar and uterine anomalies. Rarely it can be encountered in unscarred uterus. Mrs X, 35-year female, unbooked patient, G7P2L2A4 with nine months of amenorrhoea reported in emergency of RML Hospital on 30/07/2019 with history of labour pains since 2 days. Patient gave history of four dilatation and curettage for incomplete abortion. On examination patient was found to be severely anaemic (Hb -6 gm). 2 Packed RBC were transfused preoperatively. There was no progress in labour beyond 6 cm for 4 hours. Patient was thus taken for LSCS for NPOL, with blood on flow. Intraoperatively, after delivery of the baby placenta which was fundo-posterior did not separate. In view of parity and morbidly adherent placenta (clinical grade III), subtotal hysterectomy was done. Patient was transfused 4 PRBC, 4 FFP and 2 platelets. Uterus with placenta in situ was sent for histopathology. Patient was in ICU for 2 days and recovered well. Post-operative period was uneventful. Placenta accreta is defined as abnormal trophoblast invasion of whole or a part of placenta into myometrium of uterine wall. Caesarean delivery is associated with increased risk of placenta accrete and the risk increases with each caesarean section, from 0.3% in woman with one previous caesarean delivery to 6.47% for woman with five or more caesarean deliveries. Placenta accreta spectrum disorders occur in 3% of woman diagnosed with placenta previa and no prior caesarean. In the developing world, the obstetrician should be prepared to encounter un diagnosed placenta accrete even in absence of previous LSCS. Curretage following MTP is also a risk factor, so vigourous currettage should be avoided to prevent endometrial damage. more...
- Published
- 2019
- Full Text
- View/download PDF
25. Attenuation of inflammation by marine algae Turbinaria ornata in cotton pellet induced granuloma mediated by fucoidan like sulphated polysaccharide
- Author
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Meenakshi Bhardwaj, Ananthi Subash, Gayathri Veeraraghavan, Veeresh Kumar Sali, and Hannah R. Vasanthi
- Subjects
0301 basic medicine ,Polymers and Plastics ,medicine.drug_class ,Anti-Inflammatory Agents ,medicine.disease_cause ,Polysaccharide ,Phaeophyta ,Anti-inflammatory ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Polysaccharides ,Turbinaria ornata ,Materials Chemistry ,medicine ,Animals ,Cotton Fiber ,chemistry.chemical_classification ,Inflammation ,Granuloma ,Hematologic Tests ,030102 biochemistry & molecular biology ,biology ,Fucoidan ,Sulfates ,Organic Chemistry ,Glutathione ,Organ Size ,biology.organism_classification ,Molecular biology ,Bioactive compound ,Rats ,Brown algae ,Oxidative Stress ,030104 developmental biology ,chemistry ,Biochemistry ,Female ,Oxidative stress ,Biomarkers - Abstract
Turbinaria ornata, a commonly found marine brown algae along the Gulf of Mannar, Southeast coast of India was evaluated for its anti-inflammatory potential and the bioactive compound present in it was characterized. Cotton pellet induced granuloma model in rats was used to assess the anti-inflammatory potential of the aqueous extract of Turbinaria ornata (ATO) (30, 100 and 300mg/kg, p.o) which was compared with dexamethasone (0.1mg/kg, p.o) a standard anti-inflammatory agent. Granuloma weight, haematological parameters and plasma markers (LDH, GPT, and CRP) were estimated. Further, the levels of oxidative stress markers (SOD, GPx, GSH, LPO, and Nitrite) and inflammatory markers (Cathepsin D and MPO) in the hepatic tissue were measured. ATO decreased the granuloma weight dose dependently. ATO significantly reversed the levels of biochemical and inflammatory markers in comparison to the vehicle treated rats. The active constituent, fucoidan (sulphated polysaccharide) from the aqueous extract was fractionated and characterized using GCMS. The sulphated polysaccharide (TSP) from ATO confirms the presence of sulphates and sugars. The present findings suggest ATO to be a potent inhibitor of both proliferative and exudative phases of inflammation possibly mediated by the sulphated polysaccharides which might inhibit the action of COX-2 enzyme analogous to dexamethasone. more...
- Published
- 2016
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