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Membraneless organelles (MLO) regulate diverse biological processes in a spatiotemporally controlled manner spanning from inside to outside of the cells. The plasma membrane (PM) at the cell surface serves as a central platform for forming multi-component signaling hubs that sense mechanical and chemical cues during physiological and pathological conditions. During signal transduction, the assembly and formation of membrane-bound MLO are dynamically tunable depending on the physicochemical properties of the surrounding environment and partitioning biomolecules. Biomechanical properties of MLO-associated membrane structures can control the microenvironment for biomolecular interactions and assembly. Lipid-protein complex interactions determine the catalytic region’s assembly pattern and assembly rate and, thereby, the amplitude of activities. In this review, we will focus on how cell surface microenvironments, including membrane curvature, surface topology and tension, lipid-phase separation, and adhesion force, guide the assembly of PM-associated MLO for cell signal transductions.

Achieving unity quantum efficiency in single-photon emitters (SPEs) is a holy grail in quantum information science. Through plasmonic coupling it is possible to increase the quantum efficiency of SPEs by increasing the radiative decay rate, but to approach unity quantum efficiency, non-radiative decay must be mitigated. Here we show that non-radiative decay in two-dimensional WSe2 quantum emitters can be electrically suppressed through charge depletion by using dual gate configurations under a large electric field. In this condition, for site-controlled SPEs in WSe2 coupled to gold nanogaps, the SPE transition quantum efficiency after gating is increased to 76.4 ± 14.6% on average, with some SPEs reaching near-unity (more than 90%) quantum efficiency. This study provides a new approach for tuning SPEs with an applied gate voltage and motivates further theoretical and experimental studies of SPE enhancement on vertically aligned nanogaps. Non-radiative decay in two-dimensional WSe2 quantum emitters is electrically suppressed through charge depletion using dual gate configurations. The single-photon emitter transition quantum efficiency after gating is increased to 76.4 ± 14.6% on average. [ABSTRACT FROM AUTHOR]

Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However, the relevant metaphase Cdk1 targets were not known. Here we show that the highly conserved actin filament crosslinking protein fimbrin is a critical Cdk1 target for actin cable assembly regulation in budding yeast. Fimbrin is specifically phosphorylated on threonine 103 by the metaphase cyclin-Cdk1 complex, in vivo and in vitro. On the basis of conformational simulations, we suggest that this phosphorylation stabilizes fimbrin's N-terminal domain, and modulates actin filament binding to regulate actin cable assembly and stability in cells. Overall, this work identifies fimbrin as a key target for cell cycle regulation of actin cable assembly in budding yeast, and suggests an underlying mechanism.

Localized interlayer excitons (LIXs) in two-dimensional moiré superlattices exhibit sharp and dense emission peaks, making them promising as highly tunable single-photon sources. However, the fundamental nature of these LIXs is still elusive. Here, we show the donor-acceptor pair (DAP) mechanism as one of the origins of these excitonic peaks. Numerical simulation results of the DAP model agree with the experimental photoluminescence spectra of LIX in the moiré MoSe2/WSe2 heterobilayer. In particular, we find that the emission energy-lifetime correlation and the nonmonotonic power dependence of the lifetime agree well with the DAP IX model. Our results provide insight into the physical mechanism of LIX formation in moiré heterostructures and pave new directions for engineering interlayer exciton properties in moiré superlattices. The nature of localized interlayer excitons (LIXs) in moiré superlattices is still elusive Here, the authors propose a donor-acceptor pair mechanism for LIXs in MoSe2/WSe2 heterobilayers. [ABSTRACT FROM AUTHOR]

The actin cytoskeleton (AC) undergoes rapid remodelling to coordinate cellular processes during signal transduction, including changes in actin nucleation, crosslinking, and depolymerization in a time‐ and space‐dependent manner. Switching the initial actin nucleation often provides timely control of the entire actin network formation. Located at the cell surface, the plant class I formin family is a major class of actin nucleators that rapidly respond to exterior chemical and environmental cues. Plant class I formins are structurally integrated within the plant cell wall‐plasma membrane‐actin cytoskeleton (CW‐PM‐AC) continuum, sharing similar biophysical properties to mammalian integrins that are embedded within the extracellular matrix‐PM‐AC continuum. In plants, perturbation of structural components of the CW‐PM‐AC continuum changes the biophysical properties of two dimensional‐scaffolding structures, which results in uncontrolled molecular diffusion and interactions of class I formins, as well as their clustering and activities in the nucleation of the AC. Emerging studies have shown that the PM‐integrated formins are highly responsive to the mechanical perturbation of CW and AC integrity changes that tune the oligomerization and condensation of formin on the cell surface. However, during diverse signalling transductions, the molecular mechanisms that spatiotemporally underlie the mechanosensing and mechanoregulation of formin for remodelling actin remain unclear. Here, the emphasis will be placed on recent developments in understanding how the molecular condensation of class I formin regulates the biochemical activities in tuning actin polymerization during plant immune signalling, as well as how the plant structural components of the CW‐PM‐AC continuum control formin condensation at a nanometre scale. [ABSTRACT FROM AUTHOR]