Tamiru, A., Bruce, T.J.A., Woodcock, C.M., Birkett, M.A., Midega, C.A.O., Pickett, J.A., and Khan, Z.R.
A better understanding of the chemical cues employed by parasitoids to locate their herbivore hosts will contribute towards effective exploitation of parasitoids as biological control agents against damaging crop pests. The current study was conducted to establish key bioactive compounds mediating behavioural response in the parasitic wasp Cotesia sesamiae (Cameron, 1906) (Hymenoptera: Braconidae) by employing electrophysiological techniques and behavioural studies. Electroantennogram (EAG) recording with synthetic equivalents of herbivore-induced plant volatiles (HIPVs) using C. sesamiae confirmed that 13 compounds elicited electrophysiological activity. In an olfactometer bioassay, only (E)-4,8-dimethyl-1,3,7-nonatriene, (E)-β-farnesene, and (E,E)-4,8,-trimethyl-1,3,7tridecatetraene elicited behavioural responses from the parasitoid when tested individually at a natural dose, while (R)-linalool and (E)-caryophyllene were attractive at the highest test dose. Other EAG-active HIPVs were not attractive either at lower or higher concentrations, suggesting their role as blend components rather than functioning as attractants by themselves. In contrast, ninecomponent synthetic HIPV blends formulated in the same ratio and concentration as in a natural sample was as attractive as the original headspace sample. By providing further insights into maize-stemborer-parasitoid tritrophic interactions, results from this study will help in selecting crop cultivars emitting appropriate HIPVs to attract natural enemies and perhaps repel damaging crop pests. Key words: electrophysiology, bioassay, parasitoid, Cotesia sesamiae, bioactive compounds. Une meilleure comprehension des signaux chimiques employes par les parasitoides pour localiser leurs hotes herbivores facilitera leur exploitation efficace comme agents de lutte biologique contre les ravageurs des cultures. L'etude a ete realisee pour cerner les composes bioactifs cles qui modulent la reaction comportementale chez la guepe parasitique Cotesia sesamiae (Cameron, 1906) (Hymenopteres : Braconidae) en utilisant des techniques electrophysiologiques et des etudes du comportement. L'enregistrement electroantennographique (EAG) pour C. sesamiae en presence d'equivalents synthetiques de composes volatils induits chez les plantes par des herbivores (HIPV) a confirme que 13 composes suscitaient une activite electrophysiologique. Dans une epreuve biologique olfactometrique, seuls le (E)-4,8-dimethyle-1,3,7-nonatriene, le (E)-p-farnesene et le (E,E)-4,8,-trimethyle-1,3,7-tridecatetraene ont induit des reactions comportementales du parasitoide quand ils etaient testes individuellement a des doses naturelles, alors que le (R)-linalol et le (E)-caryophyllene etaient attractifs a la dose testee la plus elevee. D'autres HIPV agissant sur l'EAG n'etaient pas attractifs a des concentrations tant faibles que plus fortes, donnant a penser qu'ils jouent un role de composes de melange plutot que d'attractifs fonctionnels. En revanche, un melange d'HIPV synthetiques de neuf composes formule selon le meme rapport et les memes concentrations que ceux d'un echantillon naturel etait aussi attractif que l'echantillon d'espace de tete initial. En jetant un nouvel eclairage sur les interactions tritrophiques mais - foreur de tiges - parasitoide, les resultats de l'etude aideront a selectionner des cultivars qui emettent les bons HIPV pour attirer des ennemis naturels et, possiblement, repousser des ravageurs de cultures. [Traduit par la Redaction] Mots-cles: electrophysiologie, epreuve biologique, parasitoide, Cotesia sesamiae, composes bioactifs., Introduction When plants are subjected to insect attack, they release a blend of volatile compounds known as herbivore-induced plant volatiles (HIPVs) that attract natural enemies of the attacking herbivore (De [...]