1. Potential role of effector memory T cells in chronic T cell-mediated kidney graft rejection
- Author
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Piero Stratta, Franco Citterio, Lucrezia Furian, Fabio Sallustio, A. Toscano, Grazia Serino, Mirko Trpevski, Luigi Biancone, Loreto Gesualdo, Gianna Mazzucco, Marco Quaglia, Antonella Barreca, Paolo Rigotti, Claudia Curci, Stefania Bussolino, Giuseppe De Palma, Marco Fiorentino, Francesco Paolo Schena, Marialuisa Valente, and Michele Rossini
- Subjects
Adult ,Graft Rejection ,Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Adolescent ,T-Lymphocytes ,Settore MED/18 - CHIRURGIA GENERALE ,Lymphocyte ,T cell ,030230 surgery ,Kidney ,Young Adult ,transcriptomics ,03 medical and health sciences ,effector memory T cells ,0302 clinical medicine ,Downregulation and upregulation ,Humans ,Medicine ,IL-2 receptor ,Receptor ,Aged ,FFPE kidney biopsies ,chronic T cell-mediated rejection ,Transplantation ,business.industry ,Effector ,Middle Aged ,Receptors, OX40 ,Kidney Transplantation ,030104 developmental biology ,medicine.anatomical_structure ,Nephrology ,Female ,Transcriptome ,business ,CD8 ,Signal Transduction - Abstract
BACKGROUND Chronic T cell-mediated rejection (TCMR) in kidney graft is characterized by reduction of the vessel lumen with marked intimal thickening, fibrous hyperplasia of the small renal arteries and leukocyte infiltrates. The aim of this study was to find specific gene expression profiles in chronic TCMR kidney biopsies. METHODS RNA extracted from archival formalin-fixed, paraffin-embedded renal biopsies was used for gene expression profiling. Our study included 14 patients with chronic TCMR and 10 with acute TCMR. Fifty-two cadaveric donors were used as controls. The results were validated in an independent set of kidney biopsies. RESULTS We identified 616 and 243 differentially expressed genes with a fold change ≥1.5 and a false discovery rate
- Published
- 2016
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