5 results on '"Mohamed Kasbari"'
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2. Epidemic outbreak of anthroponotic cutaneous leishmaniasis in Kohat District, Khyber Pakhtunkhwa, Pakistan
- Author
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Hussain, Mubashir, Munir, Shahzad, Jamal, Muhammad Ameen, Ayaz, Sultan, Akhoundi, Muhammad, and Mohamed, Kasbari
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- 2017
- Full Text
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3. First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan
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Muhammad Anees, Muhammad Qasim, Muhammad Ameen Jamal, Baharullah Khattak, Taj Ali Khan, Humaira Mazhar, Sultan Ayaz, Shahzad Munir, Kashif Rahim, Mohamed Kasbari, Noha Watanay, Irfan Ahmed, Hazir Rahman, Niaz Muhammad, and Mubbashir Hussain
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Veterinary medicine ,Leishmania tropica ,biology ,030231 tropical medicine ,General Medicine ,Ribosomal RNA ,Leishmania ,biology.organism_classification ,medicine.disease ,Giemsa stain ,03 medical and health sciences ,0302 clinical medicine ,Cutaneous leishmaniasis ,parasitic diseases ,medicine ,Leishmania major ,030212 general & internal medicine ,Internal transcribed spacer ,Restriction fragment length polymorphism - Abstract
Objective To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis (CL) is endemic and was thought to be caused by Leishmania tropica only. Methods Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011–2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. Results Leishmania amastigotes were detected by microscopy in 308 of 432 samples (71.3%) while 374 out of 432 samples (86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed L. tropica in 351 and L. major in 6 biopsy samples. Conclusions This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of L. major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.
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- 2017
4. Leishmania infections: Molecular targets and diagnosis
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Mohammad Akhoundi, Katrin Kuhls, Mohamed Kasbari, Luigi Gradoni, Tim Downing, Jan Votýpka, Denis Sereno, Pierre Marty, Christophe Ravel, Pascal Delaunay, Bruno Granouillac, Julius Lukeš, Arnaud Cannet, Laboratoire de Parasitologie-Mycologie, Nice, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Université Côte d'Azur (UCA), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre méditerranéen de médecine moléculaire (C3M), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence des Leishmanioses [CHRU Montpellier] (CNR-L), Université de Montpellier (UM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques er émergentes (TransVIHMI), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Université de Bordeaux (UB)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Dublin City University [Dublin] (DCU), Institute of Parasitology, Czech Academy of Sciences [Prague] (CAS), Charles University [Prague] (CU), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Université de Montpellier (UM), Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes (TransVIHMI), Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Istituto Superiore di Sanità (ISS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université de Bordeaux (UB)
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0301 basic medicine ,Clinical Biochemistry ,Protozoan Proteins ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,Bioinformatics ,Diagnostic methods ,Biochemistry ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Leishmaniasis ,Phylogeny ,Leishmania ,Medicine(all) ,General Medicine ,Hybrid strains ,3. Good health ,Phylogeography ,Molecular Medicine ,Identification (biology) ,Genome-wide map ,Genotype ,030231 tropical medicine ,Antiprotozoal Agents ,Computational biology ,Sympatric species ,Biology ,Sensitivity and Specificity ,03 medical and health sciences ,Species Specificity ,ddc:570 ,medicine ,Animals ,Humans ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Typing ,Genotyping ,Molecular Biology ,Molecular markers ,DNA, Protozoan ,medicine.disease ,biology.organism_classification ,Leishmania Infections ,Insect Vectors ,Molecular Typing ,030104 developmental biology ,Molecular targets ,Psychodidae ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
International audience; Progress in the diagnosis of leishmaniases depends on the development of effective methods and the discovery of suitable biomarkers. We propose firstly an update classification of Leishmania species and their synonymies. We demonstrate a global map highlighting the geography of known endemic Leishmania species pathogenic to humans. We summarize a complete list of techniques currently in use and discuss their advantages and limitations. The available data highlights the benefits of molecular markers in terms of their sensitivity and specificity to quantify variation from the subgeneric level to species complexes, (sub) species within complexes, and individual populations and infection foci. Each DNA-based detection method is supplied with a comprehensive description of markers and primers and proposal for a classification based on the role of each target and primer in the detection, identification and quantification of leishmaniasis infection. We outline a genome-wide map of genes informative for diagnosis that have been used for Leishmania genotyping. Furthermore, we propose a classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans. This can be applied to newly discovered species and to hybrid strains originating from inter-species crosses. Developing more effective and sensitive diagnostic methods and biomarkers is vital for enhancing Leishmania infection control programs.
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- 2017
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5. First report of autochthonous non-vectorial canine leishmaniasis in New Caledonia, south-western Pacific: implications for new control measures and recommendations on importation of dogs
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Nathalie Daval, Mohamed Kasbari, Nicolas Keck, Laurent Guillaumot, Céline Marchal, Christophe Ravel, Thomas Hüe, Direction des Affaires Vétérinaires, Alimentaires et Rurales (DAVAR), Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP), Institut Agronomique Néo-Calédonien (IAC), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Centre National de Référence des Leishmanioses [CHRU Montpellier] (CNR-L), Université de Montpellier (UM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Départemental Vétérinaire de l'Hérault, Conseil Général de l'Hérault, Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
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MESH: Histocytochemistry ,Entomology ,Veterinary medicine ,MESH: Leishmaniasis ,Importation risk ,0403 veterinary science ,MESH: Dogs ,MESH: Communicable Disease Control ,0302 clinical medicine ,Zoonoses ,Canine leishmaniasis ,MESH: Animals ,Dog Diseases ,Leishmania infantum ,Fluorescent Antibody Technique, Indirect ,Leishmaniasis ,Transmission (medicine) ,MESH: Real-Time Polymerase Chain Reaction ,Histocytochemistry ,04 agricultural and veterinary sciences ,MESH: Leishmania infantum ,3. Good health ,Isoenzymes ,Infectious Diseases ,MESH: Isoenzymes ,MESH: Zoonoses ,Non-vectorial transmission ,040301 veterinary sciences ,MESH: Dog Diseases ,030231 tropical medicine ,Zoology ,Biology ,MESH: Disease Transmission, Infectious ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Dogs ,New Caledonia ,Transplacental and venereal transmission routes ,medicine ,Disease Transmission, Infectious ,MESH: Fluorescent Antibody Technique, Indirect ,Animals ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Direct fluorescent antibody ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,Research ,medicine.disease ,biology.organism_classification ,MESH: New Caledonia ,Parasitology ,Vector (epidemiology) ,Communicable Disease Control ,Phlebotomine sandflies ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; BACKGROUND: Canine leishmaniasis (CanL), a parasitic zoonotic disease caused by Leishmania infantum and usually transmitted by phlebotomine sandflies, has rarely been reported in Pacific islands, which have been regarded until now as leishmaniasis-free territory. Here, we report the first autochthonous CanL case in New Caledonia (south-western Pacific) and the investigations carried out 1) to determine how infection was introduced into and transmitted among these dogs and 2) to assess the risks to animal and public health.METHODS: Extensive epidemiological and entomological investigations in and around the focus were carried out. Leishmaniasis infection was confirmed by histopathology, indirect fluorescent antibody test, real-time PCR, and culture. Parasite strain was typed by the isoenzymatic technique.RESULTS: The survey revealed close contacts between the autochthonous dog and two infected bitches imported from Spain, but failed to find any possible vector or disease spreading to other animals or humans. L. infantum zymodeme MON-1, the most frequent type in the Mediterranean basin, was identified. Although transplacental and venereal transmissions could not be excluded, the evidence was in favour of non-vectorial, direct dog-to-dog transmission.CONCLUSIONS: This study corroborates the possibility of non-vectorial routes (transplacental, venereal, and direct dog-to-dog) of canine leishmaniasis transmission in New Caledonia and raises the debate of relevant test requirements and diagnostic sensitivity prior to importation of dogs in Leishmania-free regions. New leishmaniasis control measures and recommendations to avoid future CanL introduction on the island are discussed.
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- 2016
- Full Text
- View/download PDF
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