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Your search keyword '"Morrell-Falvey, Jennifer L."' showing total 38 results
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38 results on '"Morrell-Falvey, Jennifer L."'

1. Mediation of plant-mycorrhizal interaction by a lectin receptor-like kinase.

Author

Labbé, Jessy, Muchero, Wellington, Czarnecki, Olaf, Wang, Juan, Wang, Xiaoping, Bryan, Anthony C, Zheng, Kaijie, Yang, Yongil, Xie, Meng, Zhang, Jin, Wang, Dongfang, Meidl, Peter, Wang, Hemeng, Morrell-Falvey, Jennifer L, Cope, Kevin R, Maia, Lucas GS, Ané, Jean-Michel, Mewalal, Ritesh, Jawdy, Sara S, Gunter, Lee E, Schackwitz, Wendy, Martin, Joel, Le Tacon, François, Li, Ting, Zhang, Zhihao, Ranjan, Priya, Lindquist, Erika, Yang, Xiaohan, Jacobson, Daniel A, Tschaplinski, Timothy J, Barry, Kerrie, Schmutz, Jeremy, Chen, Jin-Gui, and Tuskan, Gerald A

Subjects
Mycorrhizae, Populus, Plant Roots, Protein Kinases, Plant Proteins, Symbiosis, Laccaria
Abstract

The molecular mechanisms underlying mycorrhizal symbioses, the most ubiquitous and impactful mutualistic plant-microbial interaction in nature, are largely unknown. Through genetic mapping, resequencing and molecular validation, we demonstrate that a G-type lectin receptor-like kinase (lecRLK) mediates the symbiotic interaction between Populus and the ectomycorrhizal fungus Laccaria bicolor. This finding uncovers an important molecular step in the establishment of symbiotic plant-fungal associations and provides a molecular target for engineering beneficial mycorrhizal relationships.

Published
2019

6. Amphiphilic Co-Solvents Modulate the Structure of Membrane Domains

Author

Tan, Luoxi, primary, Scott, Haden L., additional, Smith, Micholas Dean, additional, Pingali, Sai Venkatesh, additional, O’Neill, Hugh M., additional, Morrell-Falvey, Jennifer L., additional, Katsaras, John, additional, Smith, Jeremy C., additional, Davison, Brian H., additional, Elkins, James G., additional, and Nickels, Jonathan D., additional

Published
2023
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11. Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase

Author

Qiao, Zhenzhen, primary, Yates, Timothy B., additional, Shrestha, Him K., additional, Engle, Nancy L., additional, Flanagan, Amy, additional, Morrell‐Falvey, Jennifer L., additional, Sun, Yali, additional, Tschaplinski, Timothy J., additional, Abraham, Paul E., additional, Labbé, Jessy, additional, Wang, Zeng‐Yu, additional, Hettich, Robert L., additional, Tuskan, Gerald A., additional, Muchero, Wellington, additional, and Chen, Jin‐Gui, additional

Published
2021
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19. Integrated Proteomics and Lipidomics Reveal That the Swarming Motility of Paenibacillus polymyxa Is Characterized by Phospholipid Modification, Surfactant Deployment, and Flagellar Specialization Relative to Swimming Motility

Author

Poudel, Suresh, primary, Giannone, Richard J., additional, Farmer, Abigail T., additional, Campagna, Shawn R., additional, Bible, Amber N., additional, Morrell-Falvey, Jennifer L., additional, Elkins, James G., additional, and Hettich, Robert L., additional

Published
2019
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21. Labeling of Phosphatidylinositol Lipid Products in Cells through Metabolic Engineering by Using a Clickablemyo-Inositol Probe

Author

Ricks, Tanei J., primary, Cassilly, Chelsi D., additional, Carr, Adam J., additional, Alves, Daiane S., additional, Alam, Shahrina, additional, Tscherch, Kathrin, additional, Yokley, Timothy W., additional, Workman, Cameron E., additional, Morrell-Falvey, Jennifer L., additional, Barrera, Francisco N., additional, Reynolds, Todd B., additional, and Best, Michael D., additional

Published
2018
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22. Proteomics-Based Tools for Evaluation of Cell-Free Protein Synthesis

Author

Hurst, Gregory B., Asano, Keiji G., Doktycz, Charles J., Consoli, Elliot J., Doktycz, William L., Foster, Carmen M., Morrell-Falvey, Jennifer L., Standaert, Robert F., and Doktycz, Mitchel J.

Abstract

Cell-free protein synthesis (CFPS) has the potential to produce enzymes, therapeutic agents, and other proteins, while circumventing difficulties associated with in vivo heterologous expression. However, the contents of the cell-free extracts used to carry out synthesis are generally not characterized, which hampers progress toward enhancing yield or functional activity of the target protein. We explored the utility of mass spectrometry (MS)-based proteomics for characterizing the bacterial extracts used for transcribing and translating gene sequences into proteins as well as the products of CFPS reactions. Full proteome experiments identified over 1000 proteins per reaction. The complete set of proteins necessary for transcription and translation were found, demonstrating the ability to define potential metabolic capabilities of the extract. Further, MS-based techniques allowed characterization of the CFPS product and provided insight into the synthesis reaction and potential functional activity of the product. These capabilities were demonstrated using two different CFPS products, the commonly used standard green fluorescent protein (GFP, 27 kDa) and the polyketide synthase DEBS1 (394 kDa). For the large, multidomain DEBS1, substantial premature termination of protein translation was observed. Additionally, MS/MS analysis, as part of a conventional full proteomics workflow, identified post-translational modifications, including the chromophore in GFP, as well as the three phosphopantetheinylation sites in DEBS1. A hypothesis-driven approach focused on these three sites identified that all were correctly modified for DEBS1 expressed in vivo but with less complete coverage for protein expressed in CFPS reactions. These post-translational modifications are essential for functional activity, and the ability to identify them with mass spectrometry is valuable for judging the success of the CFPS reaction. Collectively, the use of MS-based proteomics will prove advantageous for advancing the application of CFPS and related techniques.

Published
2024
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28. Stochastic Assembly of Bacteria in Microwell Arrays Reveals the Importance of Confinement in Community Development

Author

Hansen, Ryan H., Timm, Collin M., Timm, Andrea C., Bible, Amber N., and Morrell-Falvey, Jennifer L.

Abstract

The structure and function of microbial communities is deeply influenced by the physical and chemical architecture of the local microenvironment and the abundance of its community members. The complexity of this natural parameter space has made characterization of the key drivers of community development difficult. In order to facilitate these characterizations, we have developed a microwell platform designed to screen microbial growth and interactions across a wide variety of physical and initial conditions. Assembly of microbial communities into microwells was achieved using a novel biofabrication method that exploits well feature sizes for control of innoculum levels. Wells with incrementally smaller size features created populations with increasingly larger variations in inoculum levels. This allowed for reproducible growth measurement in large wells, and screening for favorable growth conditions in small wells. We demonstrate the utility of this approach for screening and discovery using 5 ë_m wells to assemble P. aeruginosa colonies across a broad distribution of innoculum levels, and identify those conditions that promote the highest probability of survivial and growth under spatial confinement. Multi-member community assembly was also characterized to demonstrate the broad potential of this platform for studying the role of member abundance on microbial competition, mutualism and community succession.

Published
2016

33. A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization

Author

Bible, Amber N., primary, Fletcher, Sarah J., additional, Pelletier, Dale A., additional, Schadt, Christopher W., additional, Jawdy, Sara S., additional, Weston, David J., additional, Engle, Nancy L., additional, Tschaplinski, Timothy, additional, Masyuko, Rachel, additional, Polisetti, Sneha, additional, Bohn, Paul W., additional, Coutinho, Teresa A., additional, Doktycz, Mitchel J., additional, and Morrell-Falvey, Jennifer L., additional

Published
2016
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37. Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoeasp. YR343

Author

Estenson, Kasey, Hurst, Gregory B., Standaert, Robert F., Bible, Amber N., Garcia, David, Chourey, Karuna, Doktycz, Mitchel J., and Morrell-Falvey, Jennifer L.

Abstract

Indole-3-acetic acid (IAA) plays a central role in plant growth and development, and many plant-associated microbes produce IAA using tryptophan as the precursor. Using genomic analyses, we predicted that Pantoeasp. YR343, a microbe isolated from Populus deltoides, synthesizes IAA using the indole-3-pyruvate (IPA) pathway. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we characterized proteomes of Pantoeasp. YR343 grown in the presence of tryptophan or IAA. Exposure to IAA resulted in upregulation of proteins predicted to function in carbohydrate and amino acid transport and exopolysaccharide (EPS) biosynthesis. Metabolite profiles of wild-type cells showed the production of IPA, IAA, and tryptophol, consistent with an active IPA pathway. Finally, we constructed an ΔipdCmutant that showed the elimination of tryptophol, consistent with a loss of IpdC activity, but was still able to produce IAA (20% of wild-type levels). Although we failed to detect intermediates from other known IAA biosynthetic pathways, this result suggests the possibility of an alternate pathway or the production of IAA by a nonenzymatic route in Pantoeasp. YR343. The ΔipdCmutant was able to efficiently colonize poplar, suggesting that an active IPA pathway is not required for plant association.

Published
2018
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38. Coupled Mercury-Cell Sorption, Reduction, and Oxidation on Methylmercury Production by Geobacter sulfurreducens PCA.

Author

Hui Lin, Morrell-Falvey, Jennifer L., Rao, Balaji, Liyuan Liang, and Gu, Baohua

Subjects
*METHYLMERCURY & the environment, *GEOBACTER sulfurreducens, *MERCURY oxidation, *METHYLATION, *ENVIRONMENTAL research
Abstract

G. sulfurreducens PCA cells have been shown to reduce, sorb, and methylate Hg(II) species, but it is unclear whether this organism can oxidize and methylate dissolved elemental Hg(0) as shown for Desulfovibrio desulfuricans ND132. Using Hg(II) and Hg(0) separately as Hg sources in washed cell assays in phosphate buffered saline (pH 7.4), we report how cell-mediated Hg reduction and oxidation compete or synergize with sorption, thus affecting the production of toxic methylmercury by PCA cells. Methylation is found to be positively correlated to Hg sorption (r = 0.73) but negatively correlated to Hg reduction (r = -0.62). These reactions depend on the Hg and cell concentrations or the ratio of Hg to cellular thiols (-SH). Oxidation and methylation of Hg(0) are favored at relatively low Hg to cell-SH molar ratios (e.g., <1). Increasing Hg to cell ratios from 0.25 × 10-19 to 25 × 10-19 moles-Hg/cell (equivalent to Hg/cell-SH of 0.71 to 71) shifts the major reaction from oxidation to reduction. In the absence of five outer membrane c-type cytochromes, mutant ΔomcBESTZ also shows decreases in Hg reduction and increases in methylation. However, the presence of competing thiol-binding ions such as Zn2+ leads to increased Hg reduction and decreased methylation. These results suggest that the coupled cell-Hg sorption and redox transformations are important in controlling the rates of Hg uptake and methylation by G. sulfurreducens PCA in anoxic environments. [ABSTRACT FROM AUTHOR]

Published
2014
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