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An automatic system for cantaloupe flower pollination in greenhouses is proposed to meet the requirements of automatic pollination. The system consists of a mobile platform, robotic manipulator, and camera that reaches the flowers to detect and recognise their external features. The main task of the vision system is to detect the position and orientation of the flower in Cartesian coordinates, allowing the manipulator to reach the pose and perform pollination. A comprehensive method to ensure the accuracy of the pollination process is proposed that accurately determines the position and orientation of cantaloupe flowers in real environments. The vision system is used to capture images, detect the flower, and recognise its state according to its external features, such as size, colour, and shape, thereby providing appropriate nozzle access during pollination. The proposed approach begins with a segmentation method designed to precisely locate and segment the target cantaloupe flowers. Subsequently, a mathematical model is used to determine the key points that are important for establishing the growth orientation of each flower. Finally, an inverse-projection method is employed to convert the position of the flower from a two-dimensional (2D) image into a three-dimensional (3D) space, providing the necessary position for the pollination robot. The experimental process is conducted in a laboratory and proves the efficacy of the cantaloupe flower segmentation method, yielding precision, recall, and F1 scores of 87.91%, 90.76%, and 89.31%, respectively. Furthermore, the accuracy of the growth-orientation prediction method reaches approximately 86.7%. Notably, positional errors in 3D space predominantly fall within the allowable range, resulting in a successful pollination rate of up to 83.1%.

Nowadays, many image processing and machine learning (ML) methods are used in mango-quality classification systems. Existing algorithms perform independently to capture the relationships between features in the dataset. Nevertheless, each method has its advantages and disadvantages. As a result, this study presents an ensemble-learning framework that combines the strengths of multiple ML algorithms to make predictions. Initially, different image processing algorithms are used to extract external mango features. Next, the dataset is constructed by combining those features with weight values from sensor signals. Following that, different ML algorithms are evaluated on the dataset to determine which ones are robust. Subsequently, various ensemble-learning approaches are deployed, such as bagging, boosting, and stacking. Finally, those models are evaluated and compared, to decide which model is suited for this study’s dataset. In the experimental part, the assessment of errors demonstrates the usefulness of image processing algorithms. Furthermore, evaluation of the training models revealed that the stacking model, which integrates several methods in both the base learner and meta-learner, produced the highest results in precision, recall, F1-score, and accuracy, with values of 0.9855, 0.9901, 0.9876, and 0.9863, respectively. These experimental results confirm the robustness of the proposed methodologies for classifying mango quality.

Single-cell nanoencapsulation (SCNE) has great potential in the enhancement of therapeutic effects of probiotic microbes. However, the material scope has been limited to water-soluble compounds to avoid non-biocompatible organic solvents that are harmful to living cells. In this work, the SCNE of probiotic Lactobacillus acidophilus with water-insoluble luteolin and Fe3+ ions is achieved by the vortex-assisted, biphasic water–oil system. The process creates L. acidophilus nanoencapsulated in the luteolin-Fe3+ shells that empower the cells with extrinsic properties, such as resistance to lysozyme attack, anti-ROS ability, and α-amylase-inhibition activity, as well as sustaining viability under acidic conditions. The proposed protocol, embracing water-insoluble flavonoids as shell components in SCNE, will be an advanced add-on to the chemical toolbox for the manipulation of living cells at the single-cell level. [ABSTRACT FROM AUTHOR]

Nanoencapsulation of living cells within artificial shells is a powerful approach for augmenting the inherent capacity of cells and enabling the acquisition of extrinsic functions. However, the current state of the field requires the development of nanoshells that can dynamically sense and adapt to environmental changes by undergoing transformations in form and composition. This paper reports the compositional transformation of an enzyme‐embedded nanoshell of Fe3+‐trimesic acid complex to an iron phosphate shell in phosphate‐containing media. The cytocompatible transformation allows the nanoshells to release functional molecules without loss of activities and biorecognition, while preserving the initial shell properties, such as cytoprotection. Demonstrations include the lysis and killing of Escherichia coli by lysozyme, and the secretion of interleukin‐2 by Jurkat T cells in response to paracrine stimulation by antibodies. This work on micrometric Transformers will benefit the creation of cell‐in‐shell nanobiohybrids that can interact with their surroundings in active and adaptive ways. [ABSTRACT FROM AUTHOR]

Coordination-driven self-assembly of metal–ligand complexes is a powerful nanoarchitectonic tool for particle engineering, but its usability is limited when using two immiscible coating components. This paper reports that simple vortexing of a biphasic system of Fe3+ ions in water and flavonoids in oil forms nanoshells on individual particles, thereby enabling the utilization of water-insoluble ligands as coating materials. Mechanistic studies suggest that the biphasic mass-transfer equilibrium of flavonoid-Fe3+ species controls the shell formation, with the oil phase acting as a reservoir of coating precursors for continuous coating. The versatility and convenience of our method expand the chemical toolbox for modulating particle–material interfaces. [ABSTRACT FROM AUTHOR]

Coffee melanoidins, biocompatibility, cytoprotection, eggshell membrane hydrolysates, single-cell nanoencapsulation Keywords: coffee melanoidins; biocompatibility; cytoprotection; eggshell membrane hydrolysates; single-cell nanoencapsulation EN coffee melanoidins biocompatibility cytoprotection eggshell membrane hydrolysates single-cell nanoencapsulation 1 1 1 05/10/22 20220501 NES 220501 B Layer-by-layer (LbL) assembly b of food waste-derived materials, eggshell membrane hydrolysates and coffee melanoidins, is applied to the single-cell nanoencapsulation (SCNE) of I Saccharomyces cerevisiae i . The hydrogen bonding-based LbL process is extremely cytocompatible (viability > 99%), and the cell is protected from external assaults, such as heavy metals and UV-B, after the SCNE. [Extracted from the article]

Single-cell nanoencapsulation (SCNE) has great potential in the enhancement of therapeutic effects of probiotic microbes. However, the material scope has been limited to water-soluble compounds to avoid non-biocompatible organic solvents that are harmful to living cells. In this work, the SCNE of probiotic Lactobacillus acidophilus with water-insoluble luteolin and Fe 3+ ions is achieved by the vortex-assisted, biphasic water-oil system. The process creates L. acidophilus nanoencapsulated in the luteolin-Fe 3+ shells that empower the cells with extrinsic properties, such as resistance to lysozyme attack, anti-ROS ability, and α-amylase-inhibition activity, as well as sustaining viability under acidic conditions. The proposed protocol, embracing water-insoluble flavonoids as shell components in SCNE, will be an advanced add-on to the chemical toolbox for the manipulation of living cells at the single-cell level.

Nanoencapsulation of living cells within artificial shells is a powerful approach for augmenting the inherent capacity of cells and enabling the acquisition of extrinsic functions. However, the current state of the field requires the development of nanoshells that can dynamically sense and adapt to environmental changes by undergoing transformations in form and composition. This paper reports the compositional transformation of an enzyme-embedded nanoshell of Fe 3+ -trimesic acid complex to an iron phosphate shell in phosphate-containing media. The cytocompatible transformation allows the nanoshells to release functional molecules without loss of activities and biorecognition, while preserving the initial shell properties, such as cytoprotection. Demonstrations include the lysis and killing of Escherichia coli by lysozyme, and the secretion of interleukin-2 by Jurkat T cells in response to paracrine stimulation by antibodies. This work on micrometric Transformers will benefit the creation of cell-in-shell nanobiohybrids that can interact with their surroundings in active and adaptive ways., (© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)

Coordination-driven self-assembly of metal-ligand complexes is a powerful nanoarchitectonic tool for particle engineering, but its usability is limited when using two immiscible coating components. This paper reports that simple vortexing of a biphasic system of Fe 3+ ions in water and flavonoids in oil forms nanoshells on individual particles, thereby enabling the utilization of water-insoluble ligands as coating materials. Mechanistic studies suggest that the biphasic mass-transfer equilibrium of flavonoid-Fe 3+ species controls the shell formation, with the oil phase acting as a reservoir of coating precursors for continuous coating. The versatility and convenience of our method expand the chemical toolbox for modulating particle-material interfaces.

To investigate the structure-cellular penetration relationship of guanidinium-rich transporters (GRTs), we previously designed PGua 4 , a five-amino acid peptoid containing a conformationally restricted pattern of eight guanidines, which showed high cell-penetrating abilities and low cell toxicity. Herein, we characterized the cellular uptake selectivity, internalization pathway, and intracellular distribution of PGua 4 , as well as its capacity to deliver cargo. PGua 4 exhibits higher penetration efficiency in HeLa cells than in six other cell lines (A549, Caco-2, fibroblast, HEK293, Mia-PaCa2, and MCF7) and is mainly internalized by clathrin-mediated endocytosis and macropinocytosis. Confocal microscopy showed that it remained trapped in endosomes at low concentrations but induced pH-dependent endosomal membrane destabilization at concentrations ≥10 μM, allowing its diffusion into the cytoplasm. Importantly, PGua 4 significantly enhanced macropinocytosis and the cellular uptake and cytosolic delivery of large IgGs following noncovalent complexation. Therefore, in addition to its peptoid nature conferring high resistance to proteolysis, PGua 4 presents characteristics of a promising tool for IgG delivery and therapeutic applications.

Supramolecular self-assembly of Fe 3+ and tannic acid (TA) has received great attention in the fields of materials science and interface engineering because of its exceptional surface coating properties. Although advances in coating strategies often suggest that kinetics in the generation of interface-active Fe 3+ -TA species is deeply involved in the film formation, there is no acceptable elucidation for the coating process. In this work, we developed the enzyme-mediated kinetic control of Fe 2+ oxidation to Fe 3+ in a Fe 2+ -TA complex in the iron-gall-ink-revisited coating method. Specifically, hydrogen peroxide, produced in the glucose oxidase (GOx)-catalyzed reaction of d-glucose, accelerated Fe 2+ oxidation, and the optimized kinetics profoundly facilitated the film formation to be about 9 times thicker. We also proposed a perspective considering the coating process as nucleation and growth. From this viewpoint, the kinetics in the generation of interface-active Fe 3+ -TA species should be optimized because it determines whether the interface-active species forms a film on the substrate (i.e., heterogeneous nucleation and film growth) or flocculates in solution (i.e., homogeneous nucleation and particle growth). Moreover, GOx was concomitantly embedded into the Fe 3+ -TA films with sustained catalytic activities, and the GOx-mediated coating system was delightfully adapted to catalytic single-cell nanoencapsulation.