14 results on '"Parrado, Rudy"'
Number of results to display per page
Search Results
2. Treatment of adult chronic indeterminate Chagas disease with benznidazole and three E1224 dosing regimens: a proof-of-concept, randomised, placebo-controlled trial
- Author
-
Santina, Glaucia, Blum, Bethania, Correia, Erika, Garcia-Bournisen, Facundo, Vaillant, Michel, Morales, Jimena Ramos, Pinto Rocha, Jimy Jose, Rojas Delgadillo, Gimena, Magne Anzoleaga, Helmut Ramon, Mendoza, Nilce, Quechover, Roxana Challapa, Caballero, Maria Yurly Escobar, Lozano Beltran, Daniel Franz, Zalabar, Albert Mendoza, Rojas Panozo, Lizeth, Palacios Lopez, Alejandro, Torrico Terceros, Dunia, Fernandez Galvez, Violeta Alejandra, Cardozo, Letty, Cuellar, Gabriela, Vasco Arenas, Rudy Nelson, Gonzales, Isabel, Hoyos Delfin, Carlos Florencio, Garcia, Lineth, Parrado, Rudy, de la Barra, Anabelle, Montano, Nair, Villarroel, Sandro, Duffy, Tomas, Bisio, Margarita, Ramirez, Juan Carlos, Duncanson, Fred, Everson, Michael, Daniels, Antonia, Asada, Makoto, Cox, Eugene, Wesche, David, Diderichsen, Paul Matthias, Marques, Alexandre F, Izquierdo, Luis, Sender, Silvia Sanz, Reverter, Joan Carlos, Morales, Manuel, Jimenez, Wladimiro, Torrico, Faustino, Gascon, Joaquim, Ortiz, Lourdes, Alonso-Vega, Cristina, Pinazo, María-Jesús, Schijman, Alejandro, Almeida, Igor C, Alves, Fabiana, Strub-Wourgaft, Nathalie, and Ribeiro, Isabela
- Published
- 2018
- Full Text
- View/download PDF
3. Genetic polymorphism of Trypanosoma cruzi bloodstream populations in adult chronic indeterminate Chagas disease patients from the E1224 clinical trial
- Author
-
Ramírez, Juan Carlos, primary, Acevedo, Gonzalo Raúl, additional, Torres, Carolina, additional, Parrado, Rudy, additional, De La Barra, Anabelle, additional, Villarroel, Sandro, additional, García, Lineth, additional, Gascon, Joaquim, additional, Ortiz, Lourdes, additional, Torrico, Faustino, additional, Ribeiro, Isabela, additional, and Schijman, Alejandro Gabriel, additional
- Published
- 2021
- Full Text
- View/download PDF
4. New regimens of benznidazole monotherapy and in combination with fosravuconazole for treatment of Chagas disease (BENDITA): a phase 2, double-blind, randomised trial
- Author
-
Torrico, Faustino, primary, Gascón, Joaquim, additional, Barreira, Fabiana, additional, Blum, Bethania, additional, Almeida, Igor C, additional, Alonso-Vega, Cristina, additional, Barboza, Tayná, additional, Bilbe, Graeme, additional, Correia, Erika, additional, Garcia, Wilson, additional, Ortiz, Lourdes, additional, Parrado, Rudy, additional, Ramirez, Juan Carlos, additional, Ribeiro, Isabela, additional, Strub-Wourgaft, Nathalie, additional, Vaillant, Michel, additional, Sosa-Estani, Sergio, additional, Arteaga, Roger, additional, de la Barra, Anabelle, additional, Camacho Borja, Jhonny, additional, Martinez, Ivana, additional, Fernandes, Jayme, additional, Garcia, Lineth, additional, Lozano, Daniel, additional, Palacios, Alejandro, additional, Schijman, Alejandro, additional, Pinazo, Maria Jesus, additional, Pinto, Jimmy, additional, Rojas, Gimena, additional, Estevao, Igor, additional, Ortega-Rodriguez, Uriel, additional, Mendes, Maria Tays, additional, Schuck, Edgar, additional, Hata, Katsura, additional, Maki, Noritsugu, additional, and Asada, Makoto, additional
- Published
- 2021
- Full Text
- View/download PDF
5. Genetic polymorphism of Trypanosoma cruzi bloodstream populations in adult chronic indeterminate Chagas disease patients from the E1224 clinical trial.
- Author
-
Ramírez, Juan Carlos, Acevedo, Gonzalo Raúl, Torres, Carolina, Parrado, Rudy, Barra, Anabelle De La, Villarroel, Sandro, García, Lineth, Gascon, Joaquim, Ortiz, Lourdes, Torrico, Faustino, Ribeiro, Isabela, Schijman, Alejandro Gabriel, and De La Barra, Anabelle
- Subjects
TRYPANOSOMA cruzi ,GENETIC polymorphisms ,SATELLITE DNA ,CLINICAL trials ,CHAGAS' disease ,GENETIC variation ,GENE amplification ,GENETIC distance ,PROTOZOA ,RESEARCH ,RESEARCH methodology ,EVALUATION research ,COMPARATIVE studies ,TRYPANOSOMIASIS ,RESEARCH funding - Abstract
Background: The role that the genetic diversity of natural Trypanosoma cruzi populations plays in response to trypanocidal treatment of chronic Chagas disease (CD) patients remains to be understood. We analysed the genetic polymorphisms of parasite bloodstream populations infecting chronic CD patients enrolled in the E1224 clinical trial.Methods: A total of 506 baseline and post-treatment follow-up samples from 188 patients were analysed. T. cruzi satellite DNA (satDNA) was amplified and sequenced using cruzi1/cruzi2 primers, and samples with TcI/III, TcII, TcIV or hybrid satDNA sequences were identified. Minicircle signatures were obtained after kinetoplast DNA amplification using 121/122 primers and restriction enzyme digestion. Genetic distances between baseline and post-treatment minicircle signatures were estimated using the Jaccard coefficient.Results: At baseline, 74.3% TcII, 17.9% hybrid and 7.8% TcI/III satDNA sequences were found, whereas at the end of follow-up the distribution was 55.2% TcII, 35.2% hybrid and 9.5% TcI/III. The placebo arm was the treatment group with the highest variation of satDNA sequences between baseline and post-treatment follow-up. Genetic distances between baseline and post-treatment minicircle signatures were similar among all treatment arms. No association between minicircle signature variability and satDNA type distribution was found.Conclusions: Genetic variability of T. cruzi bloodstream populations during post-treatment follow-up did not differ from that observed during chronic infection in the absence of treatment, suggesting that there were no selection events of E1224-resistant parasite populations. This is the first report documenting the genetic polymorphism of natural T. cruzi populations in chronic patients in the context of clinical trials with trypanocidal drugs. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
6. Usefulness of Serial Blood Sampling and PCR Replicates for Treatment Monitoring of Patients with Chronic Chagas Disease
- Author
-
Parrado, Rudy, primary, Ramirez, Juan Carlos, additional, de la Barra, Anabelle, additional, Alonso-Vega, Cristina, additional, Juiz, Natalia, additional, Ortiz, Lourdes, additional, Illanes, Daniel, additional, Torrico, Faustino, additional, Gascon, Joaquim, additional, Alves, Fabiana, additional, Flevaud, Laurence, additional, Garcia, Lineth, additional, Schijman, Alejandro G., additional, and Ribeiro, Isabela, additional
- Published
- 2019
- Full Text
- View/download PDF
7. First external quality assurance program for bloodstream Real-Time PCR monitoring of treatment response in clinical trials of Chagas disease
- Author
-
Ramírez, Juan C., Parrado, Rudy, Sulleiro Igual, Elena, de la Barra, Anabelle, Rodríguez, Marcelo, Villarroel, Sandro, Irazu, Lucía, Alonso-Vega, Cristina, Alves, Fabiana, Curto, María A., García, Lineth, Ortiz, Lourdes, Torrico, Faustino, Gascon, Joaquim, Flevaud, Laurence, Molina Romero, Israel, Ribeiro, Isabela, Schijman, Alejandro G., Universitat Autònoma de Barcelona, and Universitat de Barcelona
- Subjects
0301 basic medicine ,Chagas disease ,Posaconazole ,Molecular biology ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Satellite DNA ,Ravuconazole ,Polymerase Chain Reaction ,Biochemistry ,Geographical locations ,purl.org/becyt/ford/1 [https] ,0302 clinical medicine ,Malaltia de Chagas ,Medicine and Health Sciences ,lcsh:Science ,Protozoans ,Trypanosoma Cruzi ,Multidisciplinary ,External quality assurance ,Eukaryota ,Trypanocidal Agents ,Clinical Laboratory Sciences ,3. Good health ,Nucleic acids ,Clinical Laboratories ,Nitroimidazoles ,Benznidazole ,CIENCIAS NATURALES Y EXACTAS ,Research Article ,medicine.drug ,Trypanosoma ,Bolivia ,medicine.medical_specialty ,Drug Research and Development ,Forms of DNA ,Otras Ciencias Biológicas ,Concordance ,030231 tropical medicine ,030106 microbiology ,Real-Time Polymerase Chain Reaction ,Research and Analysis Methods ,Ciencias Biológicas ,03 medical and health sciences ,Diagnostic Medicine ,Internal medicine ,parasitic diseases ,Parasitic Diseases ,Genetics ,medicine ,Humans ,Chagas Disease ,Clinical Trials ,purl.org/becyt/ford/1.6 [https] ,Molecular Biology Techniques ,Molecular Biology ,Monitoring, Physiologic ,Biologia molecular ,Pharmacology ,proficiency testing ,business.industry ,lcsh:R ,Organisms ,Biology and Life Sciences ,DNA ,Triazoles ,South America ,Molecular diagnostics ,medicine.disease ,Parasitic Protozoans ,Clinical trial ,Chagas' disease ,Immunology ,lcsh:Q ,Real-Time PCR ,Clinical Medicine ,People and places ,business ,Quality assurance - Abstract
Real-Time PCR (qPCR) testing is recommended as both a diagnostic and outcome measurement of etiological treatment in clinical practice and clinical trials of Chagas disease (CD), but no external quality assurance (EQA) program provides performance assessment of the assays in use. We implemented an EQA system to evaluate the performance of molecular biology laboratories involved in qPCR based follow-up in clinical trials of CD. An EQA program was devised for three clinical trials of CD: the E1224 (NCT01489228), a pro-drug of ravuconazole; the Sampling Study (NCT01678599), that used benznidazole, both conducted in Bolivia; and the CHAGASAZOL (NCT01162967), that tested posaconazole, conducted in Spain. Four proficiency testing panels containing negative controls and seronegative blood samples spiked with 1, 10 and 100 parasite equivalents (par. eq.)/mL of four Trypanosoma cruzi stocks, were sent from the Core Lab in Argentina to the participating laboratories located in Bolivia and Spain. Panels were analyzed simultaneously, blinded to sample allocation, at 4-month intervals. In addition, 302 random blood samples from both trials carried out in Bolivia were sent to Core Lab for retesting analysis. The analysis of proficiency testing panels gave 100% of accordance (within laboratory agreement) and concordance (between laboratory agreement) for all T. cruzi stocks at 100 par. eq./mL; whereas their values ranged from 71 to 100% and from 62 to 100% at 1 and 10 par. eq./mL, respectively, depending on the T. cruzi stock. The results obtained after twelve months of preparation confirmed the stability of blood samples in guanidine-EDTA buffer. No significant differences were found between qPCR results from Bolivian laboratory and Core Lab for retested clinical samples. This EQA program for qPCR analysis of CD patient samples may significantly contribute to ensuring the quality of laboratory data generated in clinical trials and molecular diagnostics laboratories of CD. Fil: Ramirez Gomez, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Parrado, Rudy. Universidad Mayor de San Simon; Bolivia Fil: Sulleiro, Elena. Universitat Autònoma de Barcelona; España Fil: De La Barra, Anabelle. Universidad Mayor de San Simon; Bolivia Fil: Rodríguez, Marcelo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Villarroel, Sandro. Universidad Mayor de San Simon; Bolivia Fil: Irazu, Lucía. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Alonso Vega, Cristina. Drugs for Neglected Diseases initiative; Suiza Fil: Alves, Fabiana. Drugs for Neglected Diseases initiative; Suiza Fil: Curto, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: García, Lineth. Universidad Mayor de San Simon; Bolivia Fil: Ortiz, Lourdes. Universidad Autonoma Juan Misael Saracho; Bolivia Fil: Torrico, Faustino. Fundacion CEADES; Bolivia Fil: Gascón, Joaquim. Universidad de Barcelona; España Fil: Flevaud, Laurence. Medecins Sans Frontières Operational Center Barcelona-Athens; España Fil: Molina, Israel. Universitat Autònoma de Barcelona; España Fil: Ribeiro, Isabela. Drugs for Neglected Diseases initiative; Suiza Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
- Published
- 2017
8. Real Time PCR for the Evaluation of Treatment Response in Clinical Trials of Adult Chronic Chagas Disease: Usefulness of Serial Blood Sampling and qPCR Replicates
- Author
-
Parrado, Rudy, primary, Ramirez, Juan Carlos, additional, de la Barra, Anabelle, additional, Alonso-Vega, Cristina, additional, Juiz, Natalia, additional, Ortiz, Lourdes, additional, Illanes, Daniel, additional, Torrico, Faustino, additional, Gascon, Joaquim, additional, Alves, Fabiana, additional, Flevaud, Laurence, additional, Garcia, Lineth, additional, Schijman, Alejandro G., additional, and Ribeiro, Isabela, additional
- Published
- 2018
- Full Text
- View/download PDF
9. Treatment of adult chronic indeterminate Chagas disease with benznidazole and three E1224 dosing regimens: a proof-of-concept, randomised, placebo-controlled trial
- Author
-
Torrico, Faustino, primary, Gascon, Joaquim, additional, Ortiz, Lourdes, additional, Alonso-Vega, Cristina, additional, Pinazo, María-Jesús, additional, Schijman, Alejandro, additional, Almeida, Igor C, additional, Alves, Fabiana, additional, Strub-Wourgaft, Nathalie, additional, Ribeiro, Isabela, additional, Santina, Glaucia, additional, Blum, Bethania, additional, Correia, Erika, additional, Garcia-Bournisen, Facundo, additional, Vaillant, Michel, additional, Morales, Jimena Ramos, additional, Pinto Rocha, Jimy Jose, additional, Rojas Delgadillo, Gimena, additional, Magne Anzoleaga, Helmut Ramon, additional, Mendoza, Nilce, additional, Quechover, Roxana Challapa, additional, Caballero, Maria Yurly Escobar, additional, Lozano Beltran, Daniel Franz, additional, Zalabar, Albert Mendoza, additional, Rojas Panozo, Lizeth, additional, Palacios Lopez, Alejandro, additional, Torrico Terceros, Dunia, additional, Fernandez Galvez, Violeta Alejandra, additional, Cardozo, Letty, additional, Cuellar, Gabriela, additional, Vasco Arenas, Rudy Nelson, additional, Gonzales, Isabel, additional, Hoyos Delfin, Carlos Florencio, additional, Garcia, Lineth, additional, Parrado, Rudy, additional, de la Barra, Anabelle, additional, Montano, Nair, additional, Villarroel, Sandro, additional, Duffy, Tomas, additional, Bisio, Margarita, additional, Ramirez, Juan Carlos, additional, Duncanson, Fred, additional, Everson, Michael, additional, Daniels, Antonia, additional, Asada, Makoto, additional, Cox, Eugene, additional, Wesche, David, additional, Diderichsen, Paul Matthias, additional, Marques, Alexandre F, additional, Izquierdo, Luis, additional, Sender, Silvia Sanz, additional, Reverter, Joan Carlos, additional, Morales, Manuel, additional, and Jimenez, Wladimiro, additional
- Published
- 2018
- Full Text
- View/download PDF
10. First external quality assurance program for bloodstream Real-Time PCR monitoring of treatment response in clinical trials of Chagas disease
- Author
-
Ramírez, Juan C., primary, Parrado, Rudy, additional, Sulleiro, Elena, additional, de la Barra, Anabelle, additional, Rodríguez, Marcelo, additional, Villarroel, Sandro, additional, Irazu, Lucía, additional, Alonso-Vega, Cristina, additional, Alves, Fabiana, additional, Curto, María A., additional, García, Lineth, additional, Ortiz, Lourdes, additional, Torrico, Faustino, additional, Gascón, Joaquim, additional, Flevaud, Laurence, additional, Molina, Israel, additional, Ribeiro, Isabela, additional, and Schijman, Alejandro G., additional
- Published
- 2017
- Full Text
- View/download PDF
11. Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
- Author
-
Ramírez, Juan Carlos, primary, Cura, Carolina Inés, additional, da Cruz Moreira, Otacilio, additional, Lages-Silva, Eliane, additional, Juiz, Natalia, additional, Velázquez, Elsa, additional, Ramírez, Juan David, additional, Alberti, Anahí, additional, Pavia, Paula, additional, Flores-Chávez, María Delmans, additional, Muñoz-Calderón, Arturo, additional, Pérez-Morales, Deyanira, additional, Santalla, José, additional, Marcos da Matta Guedes, Paulo, additional, Peneau, Julie, additional, Marcet, Paula, additional, Padilla, Carlos, additional, Cruz-Robles, David, additional, Valencia, Edward, additional, Crisante, Gladys Elena, additional, Greif, Gonzalo, additional, Zulantay, Inés, additional, Costales, Jaime Alfredo, additional, Alvarez-Martínez, Miriam, additional, Martínez, Norma Edith, additional, Villarroel, Rodrigo, additional, Villarroel, Sandro, additional, Sánchez, Zunilda, additional, Bisio, Margarita, additional, Parrado, Rudy, additional, Maria da Cunha Galvão, Lúcia, additional, Jácome da Câmara, Antonia Cláudia, additional, Espinoza, Bertha, additional, Alarcón de Noya, Belkisyole, additional, Puerta, Concepción, additional, Riarte, Adelina, additional, Diosque, Patricio, additional, Sosa-Estani, Sergio, additional, Guhl, Felipe, additional, Ribeiro, Isabela, additional, Aznar, Christine, additional, Britto, Constança, additional, Yadón, Zaida Estela, additional, and Schijman, Alejandro G., additional
- Published
- 2015
- Full Text
- View/download PDF
12. Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruziDNA in Blood Samples from Chagas Disease Patients
- Author
-
Ramírez, Juan Carlos, Cura, Carolina Inés, da Cruz Moreira, Otacilio, Lages-Silva, Eliane, Juiz, Natalia, Velázquez, Elsa, Ramírez, Juan David, Alberti, Anahí, Pavia, Paula, Flores-Chávez, María Delmans, Muñoz-Calderón, Arturo, Pérez-Morales, Deyanira, Santalla, José, Marcos da Matta Guedes, Paulo, Peneau, Julie, Marcet, Paula, Padilla, Carlos, Cruz-Robles, David, Valencia, Edward, Crisante, Gladys Elena, Greif, Gonzalo, Zulantay, Inés, Costales, Jaime Alfredo, Alvarez-Martínez, Miriam, Martínez, Norma Edith, Villarroel, Rodrigo, Villarroel, Sandro, Sánchez, Zunilda, Bisio, Margarita, Parrado, Rudy, Maria da Cunha Galvão, Lúcia, Jácome da Câmara, Antonia Cláudia, Espinoza, Bertha, Alarcón de Noya, Belkisyole, Puerta, Concepción, Riarte, Adelina, Diosque, Patricio, Sosa-Estani, Sergio, Guhl, Felipe, Ribeiro, Isabela, Aznar, Christine, Britto, Constança, Yadón, Zaida Estela, and Schijman, Alejandro G.
- Abstract
An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease patients. Two methods were studied: Satellite DNA (SatDNA) qPCR and kinetoplastid DNA (kDNA) qPCR. Both methods included an internal amplification control. Reportable range, analytical sensitivity, limits of detection and quantification, and precision were estimated according to international guidelines. In addition, inclusivity and exclusivity were estimated with DNA from stocks representing the different Trypanosoma cruzidiscrete typing units and Trypanosoma rangeliand Leishmaniaspp. Both methods were challenged against 156 blood samples provided by the participant laboratories, including samples from acute and chronic patients with varied clinical findings, infected by oral route or vectorial transmission. kDNA qPCR showed better analytical sensitivity than SatDNA qPCR with limits of detection of 0.23 and 0.70 parasite equivalents/mL, respectively. Analyses of clinical samples revealed a high concordance in terms of sensitivity and parasitic loads determined by both SatDNA and kDNA qPCRs. This effort is a major step toward international validation of qPCR methods for the quantification of T. cruziDNA in human blood samples, aiming to provide an accurate surrogate biomarker for diagnosis and treatment monitoring for patients with Chagas disease.
- Published
- 2015
- Full Text
- View/download PDF
13. Tratamiento de la enfermedad de Chagas crónica: evaluación parasitológica en seguimiento prolongado.
- Author
-
Fuentealba, Cristian, Zulantay, Inés, Apt, Werner, Carrasco, Daniela, García, Lineth, Parrado, Rudy, Vergara, Camilo, Saldías, Francisca, Zuñiga, Rodrigo, Martínez, Gabriela, Muñoz, Gabriela, and Valdebenito, Hugo
- Published
- 2020
14. Usefulness of Serial Blood Sampling and PCR Replicates for Treatment Monitoring of Patients with Chronic Chagas Disease
- Author
-
Parrado, Rudy, Ramirez, Juan Carlos, de la Barra, Anabelle, Alonso-Vega, Cristina, Juiz, Natalia, Ortiz, Lourdes, Illanes, Daniel, Torrico, Faustino, Gascon, Joaquim, Alves, Fabiana, Flevaud, Laurence, Garcia, Lineth, Schijman, Alejandro G., and Ribeiro, Isabela
- Abstract
This work evaluated a serial blood sampling procedure to enhance the sensitivity of duplex real-time quantitative PCR (qPCR) for baseline detection and quantification of parasitic loads and posttreatment identification of failure in the context of clinical trials for treatment of chronic Chagas disease, namely, DNDi-CH-E1224-001 (ClinicalTrials.gov registration no. NCT01489228) and the MSF-DNDi PCR Sampling Optimization Study (NCT01678599).
- Published
- 2018
- Full Text
- View/download PDF
Catalog
Books, media, physical & digital resources
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.