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10 results on '"Pasteur, N"'

1. List of Contributors

Author

Abdelbary, M.M.H., primary, Aguileta, G., additional, Alout, H., additional, Andam, C.P., additional, Arenas, M., additional, Azarian, T., additional, Baquero, F., additional, Basset, P., additional, Billmyre, R.B., additional, Biron, D.G., additional, Blanc, D.S., additional, Broset, E., additional, Byrnes, E.J., additional, Castro-Nallar, E., additional, Challagundla, L., additional, Cochran, G., additional, Cohan, F.M., additional, Comas, I., additional, Cornel, A.J., additional, D'arc, M., additional, David, J.-P., additional, Delaporte, E., additional, de Meeûs, T., additional, Djogbénou, L., additional, Dorn, P.L., additional, Dujardin, J.-P., additional, Elliot, E., additional, Etienne, L., additional, Feil, E.J., additional, Fisher, M., additional, Fouchier, R.A.M., additional, Galán, J.C., additional, Gibson, W., additional, Gilabert, A., additional, Giraud, T., additional, Gladieux, P., additional, González-Candelas, F., additional, Gonzalo-Asensio, J., additional, Hanage, W.P., additional, Harpending, H., additional, Heitman, J., additional, Hill, C.A., additional, Holzmuller, P., additional, Hurt, A.C., additional, Justi, S., additional, Kopac, Sarah, additional, Koskella, B., additional, Krafsur, E.S., additional, Labbé, P., additional, Lanzaro, G.C., additional, Lee, Y., additional, Lewis, M.D., additional, Liu, Y.-T., additional, Mahungu, T., additional, Martínez, J.L., additional, Miles, M.A., additional, Milesi, P., additional, Missé, D., additional, Morgan, A.D., additional, Morrison, D.A., additional, Nedelkov, D., additional, Owen, A., additional, Pasteur, N., additional, Peeters, M., additional, Pérez-Losada, M., additional, Polley, S.D., additional, Prugnolle, F., additional, Rasigade, J.-P., additional, Robinson, D.A., additional, Shaw, M.A., additional, Shi, Z., additional, Sutherland, C.J., additional, Tibayrenc, M., additional, van Aerle, R., additional, van der Giezen, M., additional, Van Regenmortel, M.H.V., additional, Vernet, G., additional, Vijaykrishna, D., additional, Wang, L.-F., additional, Weill, M., additional, Wirth, T., additional, and Yeo, M., additional

Published
2017
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4. Molecular data reveal a cryptic species within the Culex pipiens mosquito complex

Author

Dumas, E., Atyame, C.M., Malcolm, C.A., Le Goff, Gilbert, Unal, S., Makoundou, P., Pasteur, N., Weill, M., Duron, O., Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])

Subjects
Cell Nucleus, cytoplasmic incompatibility, Pupa, DNA, Biological Evolution, DNA, Mitochondrial, mitochondria, Culex, Haplotypes, Culex pipiens mosquito complex, Larva, parasitic diseases, [SDE]Environmental Sciences, Animals, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Symbiosis, Phylogeny, Wolbachia, Multilocus Sequence Typing
Abstract

International audience; The Culex pipiens mosquito complex is a group of evolutionarily closely related species including C. pipiens and Culex quinquefasciatus, both infected by the cytoplasmically inherited Wolbachia symbiont. A Wolbachia-uninfected population of C. pipiens was however described in South Africa and was recently proposed to represent a cryptic species. In this study, we reconsidered the existence of this species by undertaking an extensive screening for the presence of Wolbachia-uninfected C. pipiens specimens and by characterizing their genetic relatedness with known members of the complex. We first report on the presence of Wolbachia-uninfected specimens in several breeding sites. We next confirm that these uninfected specimens unambiguously belong to the C. pipiens complex. Remarkably, all uninfected specimens harbour mitochondrial haplotypes that are either novel or identical to those previously found in South Africa. In all cases, these mitochondrial haplotypes are closely related, but different, to those found in other C. pipiens complex members known to be infected by Wolbachia. Altogether, these results corroborate the presence of a widespread cryptic species within the C. pipiens species complex. The potential role of this cryptic C. pipiens species in the transmission of pathogens remains however to be determined. The designation 'Culex juppi nov. sp.' is proposed for this mosquito species.

Published
2016
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7. High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes

Author

Alout, H, Labbé, P, Berthomieu, A, Makoundou, P, Fort, P, Pasteur, N, and Weill, M

Abstract

We investigated the genetic determinism of high chlorpyrifos resistance (HCR), a phenotype first described in 1999 in Culex pipiens mosquitoes surviving chlorpyrifos doses ⩾1 mg l−1and more recently found in field samples from Tunisia, Israel or Indian Ocean islands. Through chlorpyrifos selection, we selected several HCR strains that displayed over 10 000-fold resistance. All strains were homozygous for resistant alleles at two main loci: the ace-1 gene, with the resistant ace-1Rallele expressing the insensitive G119S acetylcholinesterase, and a resistant allele of an unknown gene (named T) linked to the sex and ace-2 genes. We constructed a strain carrying only the T-resistant allele and studied its resistance characteristics. By crossing this strain with strains harboring different alleles at the ace-1 locus, we showed that the resistant ace-1Rand the T alleles act in strong synergy, as they elicited a resistance 100 times higher than expected from a simple multiplicative effect. This effect was specific to chlorpyrifos and parathion and was not affected by synergists. We also examined how HCR was expressed in strains carrying other ace-1-resistant alleles, such as ace-1Vor the duplicated ace-1Dallele, currently spreading worldwide. We identified two major parameters that influenced the level of resistance: the number and the nature of the ace-1-resistant alleles and the number of T alleles. Our data fit a model that predicts that the T allele acts by decreasing chlorpyrifos concentration in the compartment targeted in insects.

Published
2016
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8. Investigation of Cytoplasmic Incompatibility Patterns in Culex pipiens: From Field Samples to Laboratory Isofemale Lines.

Author

Namias A, Atyame C, Pasteur N, Sicard M, and Weill M

Subjects
Animals, Cytoplasm, Cytosol, Culex genetics
Abstract

This protocol describes approaches to qualify Wolbachia-induced cytoplasmic incompatibility (CI) patterns (compatible, uni or bidirectional) in crosses between two or more Culex pipiens isofemale lines, hosting different Wolbachia (wPip) strains. Here, we describe how to (1) collect the larvae in the field and grow them to the adult stage in the insectary, (2) set up isofemale lines in the insectary, (3) genetically characterize the wPip group of these lines, and (4) perform reciprocal crosses to qualify CI., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2024
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9. Dynamic of resistance alleles of two major insecticide targets in Anopheles gambiae (s.l.) populations from Benin, West Africa.

Author

Assogba BS, Pasteur N, Makoundou P, Unal S, Baba-Moussa L, Labbé P, and Weill M

Subjects
Animals, Benin, Carbamates pharmacology, Evolution, Molecular, Female, Insect Proteins genetics, Malaria, Mosquito Control methods, Mosquito Vectors drug effects, Mosquito Vectors genetics, Mutation, Organophosphates pharmacology, Permethrin pharmacology, Phenylcarbamates, Pyrethrins pharmacology, Alleles, Anopheles drug effects, Anopheles genetics, Drug Resistance, Multiple genetics, Insecticide Resistance genetics, Insecticides pharmacology
Abstract

Background: Insecticide resistance is a growing concern for malaria control and vector control effectiveness relies on assessing it distribution and understanding its evolution., Methods: We assessed resistance levels and the frequencies of two major target-site mutations, L1014F-VGSC and G119S-ace-1, conferring resistance to pyrethroids (PYRs) and carbamates/organophosphates (CXs/OPs) insecticides. These data were compared to those acquired between 2006 and 2010 to follow resistance evolutionary trends over ten years., Results: We report the results of a 3-year survey (2013-2015) of insecticide resistance in 13 localities across the whole country of Benin. Permethrin (PYR) resistance was found in all populations tested, L1014F-VGSC being almost fixed everywhere, while bendiocarb resistance was limited to a few localities, G119S-ace-1 remaining rare, with very limited variations during surveyed period. Interestingly, we found no effect of the type of insecticide pressure on the dynamics of these mutations., Conclusions: These results confirm both the high prevalence of PYR resistance and the potential of CXs/OPs as short- to medium-term alternatives in Benin. They also underline the need for regular resistance monitoring and informed management in their usage, as the G119S-ace-1 mutation is already present in Benin and surrounding countries. Their unwise usage would rapidly lead to its spread, which would jeopardize PYR-resistant Anopheles control.

Published
2020
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10. Case Report of an Anthrax Presentation Relevant to Special Operations Medicine.

Author

Winkler S, Enzenauer RW, Karesh JW, Pasteur N, Eisnor DL, Painter RB, and Calvano CJ

Subjects
Adolescent, Anthrax complications, Anthrax diagnosis, Cicatrix etiology, Eye, Facial Dermatoses diagnosis, Facial Muscles surgery, Female, Haiti, Humans, Skin Diseases, Bacterial complications, Skin Diseases, Bacterial diagnosis, Anthrax drug therapy, Anti-Bacterial Agents therapeutic use, Ceftriaxone therapeutic use, Cicatrix surgery, Facial Dermatoses drug therapy, Skin Diseases, Bacterial drug therapy
Abstract

Special Operations Forces (SOF) medical personnel function worldwide in environments where endemic anthrax (caused by Bacillus anthracis infection) may present in one of three forms: cutaneous, pulmonary, or gastrointestinal. This report presents a rare periocular anthrax case from Haiti to emphasize the need for heightened diagnostic suspicion of unusual lesions likely to be encountered in SOF theaters., (2016.)

Published
2016

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