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Your search keyword '"Pérez, Emilio M."' showing total 393 results
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393 results on '"Pérez, Emilio M."'

5. Controlled Driving of a Single-Molecule Anthracene-Based Nanocar on a Metal Surface

Author

Barragán, Ana, Lauwaet, Koen, Nicolás-García, Tomás, Sánchez-Grande, Ana, Urgel, José Ignacio, Björk, Jonas, Pérez, Emilio M., Écija, David, Joachim, Christian, Series Editor, Grill, Leonhard, Editorial Board Member, Jelezko, Fedor, Editorial Board Member, Koshino, Masanori, Editorial Board Member, Martrou, David, Editorial Board Member, Nakayama, Tomonobu, Editorial Board Member, Rapenne, Gwénaël, Editorial Board Member, Remacle, Françoise, Editorial Board Member, and Moresco, Francesca, editor

Published
2023
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7. Mechanical and liquid phase exfoliation of cylindrite: a natural van der Waals superlattice with intrinsic magnetic interactions

Author

Niu, Yue, Villalva, Julia, Frisenda, Riccardo, Sanchez-Santolino, Gabriel, Ruiz-González, Luisa, Pérez, Emilio M., García-Hernández, Mar, Burzurí, Enrique, and Castellanos-Gomez, Andres

Subjects
Physics - Applied Physics, Condensed Matter - Mesoscale and Nanoscale Physics, Condensed Matter - Materials Science
Abstract

We report the isolation of thin flakes of cylindrite, a naturally occurring van der Waals superlattice, by means of mechanical and liquid phase exfoliation. We find that this material is a heavily doped p-type semiconductor with a narrow gap (<0.85 eV) with intrinsic magnetic interactions that are preserved even in the exfoliated nanosheets. Due to its environmental stability and high electrical conductivity, cylindrite can be an interesting alternative to the existing two-dimensional magnetic materials., Comment: 5 figures in main text, 1 supp. info. figure. Available open access at the publisher's web

Published
2019
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8. Simultaneous Assembly of van der Waals Heterostructures into Multiple Nanodevices

Author

Burzurí, Enrique, Vera-Hidalgo, Mariano, Giovanelli, Emerson, Villalva, Julia, Castellanos-Gomez, Andres, and Pérez, Emilio M.

Subjects
Condensed Matter - Materials Science
Abstract

Van der Waals heterostructures (vdWH) are made of different two-dimensional (2D) layers stacked on top of each other, forming a single material with unique properties that differ from those of the individual 2D constituent layers, and that can be modulated through the interlayer interaction. These hetero-materials can be artificially made by mechanical stamping, solution processing or epitaxial growth. Alternatively, franckeite has been recently described as an example of a naturally-occurring vdWH that can be exfoliated down to nanometer thicknesses. Research on vdWHs has so far been limited to manually exfoliated and stamped individual devices. Here, a scalable and fast method to fabricate vdWH nanodevices from liquid phase exfoliated nanoflakes is reported. The transport and positioning of the flakes into localized submicrometer structures is achieved simultaneously in multiple devices via a dielectrophoretic process. The complex vdWH is preserved after dielectrophoresis and the properties of the resulting field-effect transistors are equivalent to those fabricated via mechanical exfoliation and stamping. The combination of liquid phase exfoliation and dielectrophoretic assembly is particularly suited for the study of vdWHs and applications where large-scale fabrication is required.

Published
2018
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10. Characterization of highly crystalline lead iodide nanosheets prepared by room-temperature solution processing

Author

Frisenda, Riccardo, Island, Joshua O., Lado, Jose L., Giovanelli, Emerson, Gant, Patricia, Nagler, Philipp, Bange, Sebastian, Lupton, John M., Schüller, Christian, Molina-Mendoza, Aday, Aballe, Lucia, Foerster, Michael, Korn, Tobias, Niño, Miguel Angel, de Lara, David Perez, Pérez, Emilio M., Fernandéz-Rossier, Joaquín, and Castellanos-Gomez, Andres

Subjects
Condensed Matter - Mesoscale and Nanoscale Physics, Condensed Matter - Materials Science
Abstract

Two-dimensional semiconducting materials are particularly appealing for many applications. Although theory predicts a large number of two-dimensional materials, experimentally only a few of these materials have been identified and characterized comprehensively in the ultrathin limit. Lead iodide, which belongs to the transition metal halides family and has a direct bandgap in the visible spectrum, has been known for a long time and has been well characterized in its bulk form. Nevertheless, studies of this material in the nanometer thickness regime are rather scarce. In this article we demonstrate an easy way to synthesize ultrathin, highly crystalline flakes of PbI2 by precipitation from a solution in water. We thoroughly characterize the produced thin flakes with different techniques ranging from optical and Raman spectros-copy to temperature-dependent photoluminescence and electron microscopy. We compare the results to ab initio calculations of the band structure of the material. Finally, we fabricate photodetectors based on PbI2 and study their optoelectronic properties., Comment: main text 20 pages + 10 figures, supporting information 16 pages + 17 figures

Published
2017
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11. Surfactant-Free Polar-to-Non-Polar-Phase Transfer of Exfoliated MoS2 Two-Dimensional Colloids

Author

Giovanelli, Emerson, Castellanos-Gomez, Andres, and Pérez, Emilio M.

Subjects
Condensed Matter - Mesoscale and Nanoscale Physics
Abstract

Exfoliation of lamellar materials into their corresponding layers represented a breakthrough, due to the outstanding properties arising from the nanometric thickness confinement. Among the cleavage techniques, liquid-phase exfoliation is now on the rise because it is scalable and leads to easy-to-manipulate colloids. However, all appropriate exfoliating solvents exhibit strong polarity, which restrains a lot the scope of feasible functionalization or processing of the resulting flakes. Here we propose to extend this scope, demonstrating that nanosheets exfoliated in a polar medium can be properly dispersed in a non-polar solvent. To that purpose, we prepared suspensions of molybdenum disulfide flakes in isopropanol/water and developed a phase transfer of the nanosheets to chloroform via precipitation and redispersion/centrifugation sequences, without any assisting surfactant. The colloidal stability of the nanosheets in chloroform was found to be governed by their lateral dimensions and, although lower than in polar media, proved to be high enough to open the way to subsequent functionalization or processing of the flakes in non-polar medium., Comment: 20 pages, 6 figures, 7 supporting figures

Published
2017
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13. Engineering the optoelectronic properties of MoS2 photodetectors through reversible noncovalent functionalization

Author

Molina-Mendoza, Aday J., Vaquero-Garzon, Luis, Leret, Sofia, de Juan-Fernández, Leire, Pérez, Emilio M., and Castellanos-Gomez, Andres

Subjects
Condensed Matter - Materials Science, Condensed Matter - Mesoscale and Nanoscale Physics
Abstract

We present an easy drop-casting based functionalization of MoS2-based photodetectors that results in an enhancement of the photoresponse of about four orders of magnitude, reaching responsivities up to 100 A/W. The functionalization is technologically trivial, air-stable, fully reversible and reproducible, and opens the door to the combination of 2D-materials with molecular dyes for the development of high performance photodetectors., Comment: 20 pages, main text and supporting information, 3 figures main text, 7 figures supporting information

Published
2016
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14. Franckeite: a naturally occurring van der Waals heterostructure

Author

Molina-Mendoza, Aday J., Giovanelli, Emerson, Paz, Wendel S., Niño, Miguel Angel, Island, Joshua O., Evangeli, Charalambos, Aballe, Lucía, Foerster, Michael, van der Zant, Herre S. J., Rubio-Bollinger, Gabino, Agraït, Nicolás, Palacios, J. J., Pérez, Emilio M., and Castellanos-Gomez, Andres

Subjects
Condensed Matter - Mesoscale and Nanoscale Physics, Condensed Matter - Materials Science
Abstract

The fabrication of van der Waals heterostructures, artificial materials assembled by individually stacking atomically thin (2D) materials, is one of the most promising directions in 2D materials research. Until now, the most widespread approach to stack 2D layers relies on deterministic placement methods which are cumbersome when fabricating multilayered stacks. Moreover, they tend to suffer from poor control over the lattice orientations and the presence of unwanted adsorbates between the stacked layers. Here, we present a different approach to fabricate ultrathin heterostructures by exfoliation of bulk franckeite which is a naturally occurring and air stable van der Waals heterostructure (composed of alternating SnS2-like and PbS-like layers stacked on top of each other). Presenting both an attractive narrow bandgap (<0.7 eV) and p-type doping, we find that the material can be exfoliated both mechanically and chemically down to few-layer thicknesses. We present extensive theoretical and experimental characterizations of the material's electronic properties and crystal structure, and explore applications for near-infrared photodetectors (exploiting its narrow bandgap) and for p-n junctions based on the stacking of MoS2 (n-doped) and franckeite (p-doped), Comment: 5 Figures (main text) + 19 Supp. Info. Figures

Published
2016
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17. Five Minutes in the Life of a Molecular Shuttle: Near-Equilibrium Measurements of Shuttling Dynamics Using Optical Tweezers

Author

Lemishko, Kateryna M., Naranjo, Teresa, Pérez, Emilio M., Ibarra, Borja, Joachim, Christian, Series Editor, Grill, Leonhard, Editorial Board Member, Jelezko, Fedor, Editorial Board Member, Koshino, Masanori, Editorial Board Member, Martrou, David, Editorial Board Member, Nakayama, Tomonobu, Editorial Board Member, Rapenne, Gwénaël, Editorial Board Member, and Remacle, Françoise, Editorial Board Member

Published
2020
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18. Reinforcement of Polyimine Covalent Adaptable Networks with Mechanically Interlocked Derivatives of SWNTs.

Author

Isasti, Ion, Miranda, Silvia, Jiménez, David M., Parzyszek, Sylwia, Martín Sabanés, Natalia, Pedersen, Henrik, and Pérez, Emilio M.

Subjects
ULTIMATE strength, WASTE recycling, CARBON fibers, PLASTICS, POLYMERS
Abstract

There is an urgent need for new approaches to reduce the environmental impact of plastics. One approach is to enhance recyclability. Covalent adaptable networks (CANs), where crosslinks are chemically reversible, offer an attractive alternative to thermoset materials. Another option is to strengthen polymers using nanofillers, to reduce the amount of material needed. In this regard, single‐walled carbon nanotubes (SWNTs) are excellent candidates as fillers due to their extreme strength‐to‐weight ratio and dimensionality. Here, SWNTs functionalized as mechanically‐interlocked derivatives (MINTs) are shown to significantly improve the mechanical properties of polyimine (PI) CANs, with close to optimal efficiency. Enhancements in both stiffness and ultimate strength, approaching 100% load transfer considering the SWNT loading, are observed for PI MINT, while composites made with pristine SWNTs exhibit poor improvement. The PI MINT CANs can be recycled both thermally and chemically without compromising their mechanical properties. Finally, prototype carbon fiber PI MINT laminar composites are also fabricated and characterized, demonstrating a significant increase in their mechanical properties. [ABSTRACT FROM AUTHOR]

Published
2024
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20. Rapid and simple viral protein detection by functionalized 2D MoS2/graphene electrochemiluminescence aptasensor

Author

Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Comunidad de Madrid, Ministerio de Ciencia e Innovación (España), European Commission, Ministerio de Economía y Competitividad (España), López-Diego, David [0009-0003-0396-1077], Luna, Mónica [0000-0002-7104-0726], Gutiérrez-Gálvez, Laura, El Hajioui-El Ghalbzouri, Hanaa, Enebral-Romero, Estefanía, Garrido, Marina, Naranjo, Alicia, López-Diego, David, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, Lorenzo, Encarnación, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Comunidad de Madrid, Ministerio de Ciencia e Innovación (España), European Commission, Ministerio de Economía y Competitividad (España), López-Diego, David [0009-0003-0396-1077], Luna, Mónica [0000-0002-7104-0726], Gutiérrez-Gálvez, Laura, El Hajioui-El Ghalbzouri, Hanaa, Enebral-Romero, Estefanía, Garrido, Marina, Naranjo, Alicia, López-Diego, David, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, and Lorenzo, Encarnación

Abstract

In this work we present the development of an electrochemiluminescence aptasensor based on electrografting molybdenum disulphide nanosheets functionalized with diazonium salt (MoS2-N2+) upon screen-printed electrodes of graphene (SPEs GPH) for viral proteins detection. In brief, this aptasensor consists of SPEs GPH electrografted with MoS2-N2+ and modified with a thiolated aptamer, which can specifically recognize the target protein analyte. In this case, we have used SARS-CoV-2 spike protein as model protein. Electrochemiluminescence detection was performed by using the [Ru(bpy)3]2+/TPRA (tripropylamine) system, which allows the specific detection of the SARS-CoV-2 spike protein easily and rapidly with a detection limit of 9.74 fg/mL and a linear range from 32.5 fg/mL to 50.0 pg/mL. Moreover, the applicability of the aptasensor has been confirmed by the detection of the protein directly in human saliva samples. Comparing our device with a traditional saliva antigen test, our aptasensor can detect the spike protein even when the saliva antigen test gives a negative result.

Published
2024

25. Multiplex Portable Biosensor for Bacteria Detection

Author

Kaci, Karim, primary, Enebral-Romero, Estefanía, additional, Martínez-Periñán, Emiliano, additional, Garrido, Marina, additional, Pérez, Emilio M., additional, López-Diego, David, additional, Luna, Mónica, additional, González-de-Rivera, Guillermo, additional, García-Mendiola, Tania, additional, and Lorenzo, Encarnación, additional

Published
2023
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28. Scope and Limitations of Using Microemulsions for the Covalent Patterning of Graphene.

Author

Naranjo, Alicia, Garrido, Marina, Martín Sabanés, Natalia, and Pérez, Emilio M.

Subjects
GRAPHENE, ELECTRONIC structure, MICROEMULSIONS, EMULSIONS, MICELLES
Abstract

Patterning of graphene (functionalizing some areas while leaving others intact) is challenging, as all the C atoms in the basal plane are identical, but it is also desirable for a variety of applications, like opening a bandgap in the electronic structure of graphene. Several methods have been reported to pattern graphene, but most of them are very technologically intensive. Recently, we reported the use of microemulsions as templates to pattern graphene at the μm scale. This method is very simple and in principle tunable, as emulsions of different droplet size and composition can be prepared easily. Here, we explore in detail the scope of this methodology by applying it to all the combinations of four different emulsions and three different organic reagents, and characterizing the resulting substrates exhaustively through Raman, SEM and AFM. We find that the method is general, works better when the reactive species are outside the micelles, and requires reactive species that involve short reaction times. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Interlocking Matrix and Filler for Enhanced Individualization and Reinforcement in Polymer–Single-Walled Carbon Nanotube Composites

Author

Villalva, Julia, primary, Rapakousiou, Amalia, additional, Monclús, Miguel A., additional, Fernández Blázquez, Juan Pedro, additional, de la Vega, Jimena, additional, Naranjo, Alicia, additional, Vera-Hidalgo, Mariano, additional, Ruiz-González, María Luisa, additional, Pedersen, Henrik, additional, and Pérez, Emilio M., additional

Published
2023
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32. Data - Automated Statistical analysis of Raman spectra of nanomaterials

Author

Martín Sabanés, N., Eaton, M. D., Moreno-Da Silva, S., Naranjo, A., Pérez, Emilio M., Martín Sabanés, N., Eaton, M. D., Moreno-Da Silva, S., Naranjo, A., and Pérez, Emilio M.

Abstract

A computational tool to automatically analyze the Raman spectral features of nanomaterials. Codes and experimental data.

Published
2023

33. Direct Magnetic Evidence, Functionalization, and Low-Temperature Magneto-Electron Transport in Liquid-Phase Exfoliated FePS3

Author

Martín-Pérez, Lucas, Medina Rivero S., Vázquez Sulleiro M., Naranjo A., Gómez I.J., Ruíz-González M.L., Castellanos-Gomez A., Garcia-Hernandez M., Pérez, Emilio M., Burzurí E., Martín-Pérez, Lucas, Medina Rivero S., Vázquez Sulleiro M., Naranjo A., Gómez I.J., Ruíz-González M.L., Castellanos-Gomez A., Garcia-Hernandez M., Pérez, Emilio M., and Burzurí E.

Published
2023

34. Microwave-Driven Exfoliation of Bulk 2H-MoS2 after Acetonitrile Prewetting Produces Large-Area Ultrathin Flakes with Exceptionally High Yield

Author

Quirós-Ovies, Ramiro, Laborda, María, Martín Sabanés, Natalia, Martín-Pérez, Lucía, Moreno-Da Silva, Sara, Burzurí E., Sebastian, Víctor, Pérez, Emilio M., Santamaría, Jesús, Quirós-Ovies, Ramiro, Laborda, María, Martín Sabanés, Natalia, Martín-Pérez, Lucía, Moreno-Da Silva, Sara, Burzurí E., Sebastian, Víctor, Pérez, Emilio M., and Santamaría, Jesús

Published
2023

36. Pathogen sensing device based on 2D MoS2/graphene heterostructure.

Author

Enebral-Romero E., Gutiérrez-Gálvez L., Del Caño R., Sulleiro M.V., Naranjo A., Gómez I.J., Pariente F., Pérez, Emilio M., García-Mendiola T., Lorenzo E., Enebral-Romero E., Gutiérrez-Gálvez L., Del Caño R., Sulleiro M.V., Naranjo A., Gómez I.J., Pariente F., Pérez, Emilio M., García-Mendiola T., and Lorenzo E.

Published
2023

38. Interlocking Matrix and Filler for Enhanced Individualization and Reinforcement in Polymer-Single-Walled Carbon Nanotube Composites

Author

Villalva J., Rapakousiou A., Monclús M.A., Fernández Blázquez J.P., de la Vega J., Naranjo A., Vera-Hidalgo M., Ruiz-González M.L., Pedersen H., Pérez, Emilio M., Villalva J., Rapakousiou A., Monclús M.A., Fernández Blázquez J.P., de la Vega J., Naranjo A., Vera-Hidalgo M., Ruiz-González M.L., Pedersen H., and Pérez, Emilio M.

Published
2023

40. Supplementary Material MoS2-Carbon Nanodots as New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection

Author

Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, Lorenzo, Encarnación, Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, and Lorenzo, Encarnación

Published
2023

41. Multiplex Portable Biosensor for Bacteria Detection

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, Ministerio de Economía y Competitividad (España), European Commission, Kaci, Karim, Enebral-Romero, Estefanía, Martínez-Periñán, Emiliano, Garrido, Marina, Pérez, Emilio M., López-Diego, David, Luna, Mónica, González de Rivera, Guillermo, García-Mendiola, Tania, Lorenzo, Encarnación, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, Ministerio de Economía y Competitividad (España), European Commission, Kaci, Karim, Enebral-Romero, Estefanía, Martínez-Periñán, Emiliano, Garrido, Marina, Pérez, Emilio M., López-Diego, David, Luna, Mónica, González de Rivera, Guillermo, García-Mendiola, Tania, and Lorenzo, Encarnación

Abstract

An advanced, cost-effective, and portable DNA biosensor capable of detecting multiple bacteria simultaneously has been developed. The biosensor comprises a fast and inexpensive potentiostat that controls the applied potential to a screen-printed electrochemical array platform functionalized with MoS2 flakes and bacterial DNA probes. The current response obtained by à la carte thionine functionalized carbon nanodots (Ty-CDs) is monitored as an electrochemical indicator of the hybridization event. The design of the potentiostat prioritizes achieving an optimal signal-to-noise ratio and incorporates a user-friendly interface compatible with various devices, including computers, mobile phones, and tablets. The device is compact, lightweight, and manufactured at a low cost. The key components of the potentiostat include a data acquisition board capable of analyzing multiple samples simultaneously and a controller board. The results of this study confirm the ability of the multiplex portable biosensor to successfully detect specific bacterial DNA sequences, demonstrating its reliability and superior performance compared with a traditional, more complex, and laboratory-oriented potentiostat.

Published
2023

42. Supporting information: Microwave-Driven Exfoliation of Bulk 2H-MoS2 after Acetonitrile Prewetting Produces Large-Area Ultrathin Flakes with Exceptionally High Yield

Author

Quirós‐Ovies, Ramiro, Laborda, María, Martín Sabanés, Natalia, Martín-Pérez, Lucía, Moreno-Da Silva, Sara, Burzurí, Enrique, Sebastián, Víctor, Pérez, Emilio M., Santamaría, Jesús, Quirós‐Ovies, Ramiro, Laborda, María, Martín Sabanés, Natalia, Martín-Pérez, Lucía, Moreno-Da Silva, Sara, Burzurí, Enrique, Sebastián, Víctor, Pérez, Emilio M., and Santamaría, Jesús

Abstract

Screening of exfoliation conditions (dispersion solvent, prewetting solvent, microwave power, microwave irradiation time, MoS2–NMP ratio, and % yield); UV–vis analyses, optical and electronic microscopy analyses; thermogravimetric analysis of bulk and exfoliated MoS2, X-ray photoelectron spectroscopy analysis, electron transport measurements.

Published
2023

43. Bridging spin Qbits to superconducting circuits through carbon nanotubes

Author

Ory, Marina C. de, Moreno-Da Silva, Sara, Martín-Pérez, Lucía, Pérez, Emilio M., Granados, Daniel, Luis, Fernando, Burzurí, Enrique, Gómez, Alicia, Ory, Marina C. de, Moreno-Da Silva, Sara, Martín-Pérez, Lucía, Pérez, Emilio M., Granados, Daniel, Luis, Fernando, Burzurí, Enrique, and Gómez, Alicia

Abstract

Hybrid systems based on superconducting planar resonators strongly coupled to magnetic molecules are promising candidates for future large-scale quantum processors. Superconducting microwave resonators allow the strong confinement of the electromagnetic fields mode volume, which is crucial for reaching a strong and coherent coupling with the spin qubits. However, achieving control over a few or single spins remains challenging. In this sense, two main issues need to be overcome. First, the cavity field effective volume needs to be further concentrated to maximize the addressability of a single spin in a controlled way. Second, as the microwave field is localized in a nanoscopic region, developing a method to deliver the magnetic sample with sufficient spatial accuracy is crucial. As a first approach, I. Gimeno et al. showed that reducing the width of a superconducting transmission line locally leads to an enhancement of the microwave magnetic field and, therefore, of the achieved coupling. Utilizing dip pen nanolithography, they can place nanodeposits in a controlled way, reducing the number of coupled spins by several orders of magnitude. However, new approaches must be developed to achieve strong coupling to a single spin. We propose to downscale and reach the single-spin sensitivity limit by replacing the constriction of the superconducting resonator with a carbon nanotube (CNT). This approach presents two significant advantages: first, it concentrates further the microwave magnetic volume, increasing the coupling rate to a single spin; second, as CNT can be directly functionalized with molecular species, it allows addressing a single magnetic molecule in a localized position. Preliminary results of a hybrid superconducting/CNT resonator are presented, where the controlled positioning of the carbon nanotube on the nanometer scale has been achieved by dielectrophoresis. Moreover, optimized superconducting lumped element resonators (LERs) have been developed and co

Published
2023

44. MoS2-Carbon nanodots as a new electrochemiluminescence platform for breast cancer biomarker detection

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Consejo Superior de Investigaciones Científicas (España), European Commission, Ministerio de Ciencia e Innovación (España), Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, Lorenzo, Encarnación, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Consejo Superior de Investigaciones Científicas (España), European Commission, Ministerio de Ciencia e Innovación (España), Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, and Lorenzo, Encarnación

Abstract

In this work, we present the combination of two different types of nanomaterials, 2D molybdenum disulfide nanosheets (MoS2-NS) and zero-dimensional carbon nanodots (CDs), for the development of a new electrochemiluminescence (ECL) platform for the early detection and quantification of the biomarker human epidermal growth factor receptor 2 (HER2), whose overexpression is associated with breast cancer. MoS2-NS are used as an immobilization platform for the thiolated aptamer, which can recognize the HER2 epitope peptide with high affinity, and CDs act as coreactants of the anodic oxidation of the luminophore [Ru(bpy)3]2+. The HER2 biomarker is detected by changes in the ECL signal of the [Ru(bpy)3]2+/CD system, with a low detection limit of 1.84 fg/mL and a wide linear range. The proposed method has been successfully applied to detect the HER2 biomarker in human serum samples.

Published
2023

48. Pathogen sensing device based on 2D MoS2/graphene heterostructure

Author

Enebral Romero, Estefanía, Gutiérrez Gálvez, Laura, Del Caño Ochoa, Rafael, Vázquez Sulleiro, Manuel, Naranjo, Alicia, Gómez, I. Jénnifer, Pariente Alonso, Félix, Pérez, Emilio M., García Mendiola, Tania, Lorenzo Abad, Encarnación, and UAM. Departamento de Química Analítica y Análisis Instrumental

Subjects
SARS-CoV-2, Electrografting, Covalent heterostructures, Aptasensor, Química, f-MoS 2
Abstract

In this work we propose a new methodology for selective and sensitive pathogen detection based on a 2D layered heterostructured biosensing platform. As a proof of concept, we have chosen SARS-CoV-2 virus because the availability of new methods to detect this virus is still a great deal of interest. The prepared platform is based on the covalent immobilization of molybdenum disulphide functionalized with a diazonium salt (f-MoS2) onto graphene screen-printed electrodes (GPH SPE) by electrografting of the diazonium salt. This chemistry-based method generates an improved heterostructured biosensing platform for aptamer immobilization and aptasensor development. Electrochemical impedance spectroscopy (EIS) is used to obtain the signal response of the device, proving the ability of the sensor platform to detect the virus. SARS-CoV-2 spike RBD recombinant protein (SARS-CoV-2 S1 protein) has been detected and quantified with a low detection limit of 2.10 fg/mL. The selectivity of the developed biosensor has been confirmed after detecting the S1 protein even in presence of other interfering proteins. Moreover, the ability of the device to detect SARS-CoV-2 S1 protein has been also tested in nasopharyngeal swab samples, This work has been financially supported by the Spanish Ministry of Economy and Competitiveness (PID2020-116728RB-I00, PID2020- 116661RB-I00, CTQ2015-71955-REDT (ELECTROBIONET)) and Community of Madrid (TRANSNANOAVANSENS, S2018/NMT-4349, and PhotoArt P2018/NMT-4367). E. Enebral thank the financial support of “Nanotecnología para detección del SARS-CoV-2 y sus variantes. NANOCOV” project. IMDEA Nanociencia receives support from the “Severo Ochoa” Programme for Centres of Excellence in R&D (MINECO, Grant CEX2020-001039-S). We also thank the Spanish Ministry of Universities for supporting Laura Gutiérrez-Galvez with the Formación del Profesorado Universitario (FPU) grant (FPU19/06309)

Published
2023

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