12 results on '"Peng, Yongbin"'
Search Results
2. Identification and Characterization of miRNAs and lncRNAs Associated with Salinity Stress in Rice Panicles.
- Author
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Jiang, Conghui, Wang, Yulong, He, Yanan, Peng, Yongbin, Xie, Lixia, Li, Yaping, Sun, Wei, Zhou, Jinjun, Zheng, Chongke, and Xie, Xianzhi
- Subjects
COMPETITIVE endogenous RNA ,GENE expression ,HEAT shock proteins ,LINCRNA ,GERMPLASM ,CAROTENOIDS ,RICE breeding - Abstract
Salinity is a common abiotic stress that limits crop productivity. Although there is a wealth of evidence suggesting that miRNA and lncRNA play important roles in the response to salinity in rice seedlings and reproductive stages, the mechanism by which competing endogenous RNAs (ceRNAs) influence salt tolerance and yield in rice has been rarely reported. In this study, we conducted full whole-transcriptome sequencing of rice panicles during the reproductive period to clarify the role of ceRNAs in the salt stress response and yield. A total of 214 lncRNAs, 79 miRNAs, and 584 mRNAs were identified as differentially expressed RNAs under salt stress. Functional analysis indicates that they play important roles in GO terms such as response to stress, biosynthesis processes, abiotic stimuli, endogenous stimulus, and response to stimulus, as well as in KEGG pathways such as secondary metabolite biosynthesis, carotenoid biosynthesis, metabolic pathways, and phenylpropanoid biosynthesis. A ceRNA network comprising 95 lncRNA–miRNA–mRNA triplets was constructed. Two lncRNAs, MSTRG.51634.2 and MSTRG.48576.1, were predicted to bind to osa-miR172d-5p to regulate the expression of OsMYB2 and OsMADS63, which have been reported to affect salt tolerance and yield, respectively. Three lncRNAs, MSTRG.30876.1, MSTRG.44567.1, and MSTRG.49308.1, may bind to osa-miR5487 to further regulate the expression of a stress protein (LOC_Os07g48460) and an aquaporin protein (LOC_Os02g51110) to regulate the salt stress response. This study is helpful for understanding the underlying molecular mechanisms of ceRNA that drive the response of rice to salt stress and provide new genetic resources for salt-resistant rice breeding. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Moderate Salinity Stress Affects Rice Quality by Influencing Expression of Amylose- and Protein-Content-Associated Genes.
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Zheng, Chongke, Niu, Shulin, Yan, Ying, Zhou, Guanhua, Peng, Yongbin, He, Yanan, Zhou, Jinjun, Li, Yaping, and Xie, Xianzhi
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RICE quality ,SALINITY ,ELECTRIC conductivity ,GENES ,GRAIN yields ,RICE ,LAMINARIA - Abstract
Salinity is an environmental stress that severely impacts rice grain yield and quality. However, limited information is available on the molecular mechanism by which salinity reduces grain quality. In this study, we investigated the milling, appearance, eating and cooking, and nutritional quality among three japonica rice cultivars grown either under moderate salinity with an electrical conductivity of 4 dS/m or under non-saline conditions in a paddy field in Dongying, Shandong, China. Moderate salinity affected rice appearance quality predominantly by increasing chalkiness rate and chalkiness degree and affected rice eating and cooking and nutritional quality predominantly by decreasing amylose content and increasing protein content. We compared the expression levels of genes determining grain chalkiness, amylose content, and protein content in developing seeds (0, 5, 10, 15, and 20 days after flowering) of plants grown under saline or non-saline conditions. The chalkiness-related gene Chalk5 was up-regulated and WHITE-CORE RATE 1 was repressed. The genes Nuclear factor Y and Wx, which determine amylose content, were downregulated, while protein-content-associated genes OsAAP6 and OsGluA2 were upregulated by salinity in the developing seeds. These findings suggest some target genes that may be utilized to improve the grain quality under salinity stress conditions via gene-pyramiding breeding approaches. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Genome-Wide Scan for Genetic Signatures Based on the Whole-Genome Resequencing of Salt- and Drought-Tolerant Rice Varieties
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Jiang, Conghui, primary, Wang, Yulong, additional, Zhou, Jinjun, additional, Rashid, Muhammad Abdul Rehman, additional, Li, Yaping, additional, Peng, Yongbin, additional, Xie, Lixia, additional, Zhou, Guanhua, additional, He, Yanan, additional, Sun, Wei, additional, Zheng, Chongke, additional, and Xie, Xianzhi, additional
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- 2023
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5. Moderate Salinity Stress Reduces Rice Grain Yield by Influencing Expression of Grain Number- and Grain Filling-Associated Genes
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Guanhua Zhou, Chongke Zheng, Peng Yongbin, Xianzhi Xie, Wen Li, and Zhizhen Zhang
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0106 biological sciences ,0301 basic medicine ,Soil salinity ,food and beverages ,Plant physiology ,Plant Science ,Biology ,01 natural sciences ,Salinity ,03 medical and health sciences ,030104 developmental biology ,Human fertilization ,Agronomy ,Paddy field ,Cultivar ,Agronomy and Crop Science ,Gene ,010606 plant biology & botany ,Panicle - Abstract
Soil salinity is an environmental stress severely impacting on rice grain yield. However, limited information is available on how salinity affects expression levels of genes determining grain yield. In this study, we investigated agronomic traits associated with grain yield among three japonica rice cultivars grown either under moderate salinity with an electrical conductivity of 4 dS/m or under non-saline conditions in a paddy field in Dongying, Shandong, China. Moderate salinity affected rice yield predominantly by reducing grain number and grain filling. We compared expression levels of genes determining grain number in young panicles (0.5–1 cm in length) of plants grown under salinity or non-saline conditions. Transcription of Lax panicle 1, Lax panicle 2, Ideal plant architecture (IPA1), Dense and erect panicle 1, Tawawa 1, and OsMADS1 was significantly repressed, whereas that of Cytokinin oxidase/dehydrogenase, Grain number per panicle 1, and Narrow leaf 1 was not significantly influenced by salinity. OsmicroRNA156, the posttranscriptional regulator of IPA1, was induced by salinity in the panicle and seedlings and complemented the expression patterns of IPA1. This result implied that the OsmicroRNA156–IPA1 pathway was involved in rice salinity responses. The grain filling-associated genes Grain incomplete filling 1, Grain incomplete filling 2, and Nuclear factor Y were down-regulated by salinity in the spikelet at 5 or 10 days after fertilization, which contributed to the salinity-triggered reduction in grain weight. These findings suggest some targets that may be utilized to improve the grain yield under salinity stress conditions via breeding and gene-pyramiding approaches.
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- 2020
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6. Moderate Salinity Stress Reduces Rice Grain Yield by Influencing Expression of Grain Number- and Grain Filling-Associated Genes
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Zheng, Chongke, primary, Zhou, Guanhua, additional, Zhang, Zhizhen, additional, Li, Wen, additional, Peng, Yongbin, additional, and Xie, Xianzhi, additional
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- 2020
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7. Identification and gene mapping of a grain-filling mutant gef1 in rice
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Peng Yongbin, Hongyu Zhang, KangXi Du, QingShan Jiang, Yao Fu, Pei-zhou Xu, TingKai Wu, Xian-jun Wu, Baolin Han, JiaBin Chen, Xiaoqiong Chen, and DeMing Zhao
- Subjects
education.field_of_study ,Multidisciplinary ,Population ,Glume ,Mutant ,Wild type ,food and beverages ,Biology ,Vascular bundle ,Caryopsis ,Botany ,Amyloplast ,education ,Plant stem - Abstract
Grain filling is an extremely important process in rice growth and development. This process which involves complex genetic regulatory networks and environmental interaction, is directly related to the formation of rice yield and quality. A stable inherited mutant with grain extended filling ( gef1 ) was identified from an EMS mutagenized population of Yixiang 1B. The investigation of agronomic traits, measurement of physiological and biochemical characteristics, histology observation, gene expression analysis and gene mapping were performed in this study. Compared with the wild type plants, the grain filling rate of gef1 was significantly lower than that of Yixiang 1B, resulting in the grain filling time of gef1 was about 30 days delay. Meanwhile, the 1000-grain weight of gef1 became bigger and seed setting rate decreased by 22%. During the development of caryopsis, we found that the soluble sugar content in wild type showed a single peak curve, and the content maintained at a low level throughout the grain filling stage. However, the soluble sugar content of the gef1 was significantly higher than that of the wild type, and showing a bimodal curve. It revealed that the transportation and distribution of photosynthate, and starch synthesis were inefficiency in gef1 . In addition, histocytological analysis showed that the number of big vascular bundles in the same transection parts of rachis, the first top internodes and the second top internodes were all decreased in gef1 , and indicated that the transport efficiency of photosynthate was one of the limiting factors in the rate of grain filling. The scanning electron microscope showed that the glume of gef1 had smooth surface and slender cells. Scanning of grain transection showed that gef1 had spherical amyloplasts and arranged loosely, but the wild type had unregular polygon amyloplasts and packed closely. These results suggested that the changes of grain size associated with the cell number and shape of glume, and gef1 had impact on rice quality. Additionally, by analyzing the expression of the glycometabolism-related genes in developing caryopsis, we found that all of them were keeping at a low level in gef1 compared with the wild type. By analyzing the three crosses gef1 /Yixiang 1B, gef1 /02428 and gef1 /Kitaake, we found that the F1 plants of these three crosses were all normal and indicated that the mutant trait was controlled by a recessive gene. The ratio of normal plants to mutant plants in the three F2 populations was 3:1 and indicating that the mutant trait was controlled by a single recessive nuclear gene. gef1 was preliminarily mapped on the short arm of rice chromosome 3, and was narrowed to a 198 kb region between InDel3-1 and InDel3-2. This region has 21 open reading frames and there was no homologous gene had been reported within it, and the further identification work of candidate genes is ongoing.
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- 2016
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8. Rice Calcineurin B-Like Protein-Interacting Protein Kinase 31 (OsCIPK31) Is Involved in the Development of Panicle Apical Spikelets
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Xiaoshu Deng, Asif Ali, Xianjun Wu, Chaojian Gu, Yongxiang Liao, Peizhou Xu, Peng Yongbin, Tingkai Wu, Feixue Hou, Xiaoqiong Chen, Hongyu Zhang, and Bai Que
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0106 biological sciences ,0301 basic medicine ,Apical dominance ,Population ,Mutant ,MAPK signaling ,Plant Science ,lcsh:Plant culture ,Biology ,01 natural sciences ,apical dominance ,03 medical and health sciences ,Auxin ,lcsh:SB1-1110 ,CIPK ,Protein kinase A ,education ,panicle apical abortion ,Gene ,Original Research ,Panicle ,chemistry.chemical_classification ,education.field_of_study ,IAA ,food and beverages ,ROS ,Cell biology ,Complementation ,030104 developmental biology ,chemistry ,auxin ,010606 plant biology & botany - Abstract
Panicle apical abortion (PAA) causes severe yield losses in rice production, but details about its development and molecular basis remain elusive. Herein, a PAA mutant, paa1019, was identified among the progeny of an elite indica maintainer rice line Yixiang 1B (YXB) mutagenized population obtained using ethyl methyl sulfonate. The abortion rate of spikelets in paa1019 was observed up to 60%. Genetic mapping combined with Mutmap analysis revealed that LOC_Os03g20380 harbored a single-bp substitution (C to T) that altered its transcript length. This gene encodes calcineurin B-like protein-interacting protein kinase 31 (OsCIPK31) localized into the cytoplasm, and is preferentially expressed in transport tissues of rice. Complementation of paa1019 by transferring the open reading frame of LOC_Os03g20380 from YXB reversed the mutant phenotype, and conversely, gene editing by knocking out of OsCIPK31 in YXB results in PAA phenotype. Our results support that OsCIPK31 plays an important role in panicle development. We found that dysregulation is caused by the disruption of OsCIPK31 function due to excessive accumulation of ROS, which ultimately leads to cell death in rice panicle. OsCIPK31 and MAPK pathway might have a synergistic effect to lead ROS accumulation in response to stresses. Meanwhile the PAA distribution is related to IAA hormone accumulation in the panicle. Our study provides an understanding of the role of OsCIPK31 in panicle development by responding to various stresses and phytohormones.
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- 2018
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9. Mutation in Rice Abscisic Acid2 Results in Cell Death, Enhanced Disease-Resistance, Altered Seed Dormancy and Development
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Peng Yongbin, Tingkai Wu, Peizhou Xu, Xiaoshu Deng, Wenming Wang, Xianjun Wu, Daiming Guo, Yongxiang Liao, Bai Que, Xiaoqiong Chen, Hongyu Zhang, Asif Ali, and Ming Luo
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0106 biological sciences ,0301 basic medicine ,Programmed cell death ,Mutant ,Plant Science ,Biology ,Plant disease resistance ,lcsh:Plant culture ,medicine.disease_cause ,01 natural sciences ,Lesion ,abscisic acid ,03 medical and health sciences ,chemistry.chemical_compound ,Downregulation and upregulation ,medicine ,lcsh:SB1-1110 ,Oryza sativa L ,xanthoxin dehydrogenase ,Abscisic acid ,Original Research ,Mutation ,pre-harvest sprouting ,Point mutation ,food and beverages ,ABA/GA ratio ,gibberellin ,Cell biology ,030104 developmental biology ,chemistry ,lesion mimic mutant ,defense responses ,medicine.symptom ,010606 plant biology & botany - Abstract
Lesion mimic mutants display spontaneous cell death, and thus are valuable for understanding the molecular mechanism of cell death and disease resistance. Although a lot of such mutants have been characterized in rice, the relationship between lesion formation and abscisic acid (ABA) synthesis pathway is not reported. In the present study, we identified a rice mutant, lesion mimic mutant 9150 (lmm9150), exhibiting spontaneous cell death, pre-harvest sprouting, enhanced growth, and resistance to rice bacterial and blast diseases. Cell death in the mutant was accompanied with excessive accumulation of H2O2. Enhanced disease resistance was associated with cell death and upregulation of defense-related genes. Map-based cloning identified a G-to-A point mutation resulting in a D-to-N substitution at the amino acid position 110 of OsABA2 (LOC_Os03g59610) in lmm9150. Knock-out of OsABA2 through CRISPR/Cas9 led to phenotypes similar to those of lmm9150. Consistent with the function of OsABA2 in ABA biosynthesis, ABA level in the lmm9150 mutant was significantly reduced. Moreover, exogenous application of ABA could rescue all the mutant phenotypes of lmm9150. Taken together, our data linked ABA deficiency to cell death and provided insight into the role of ABA in rice disease resistance.
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- 2018
10. Rice Calcineurin B-Like Protein-Interacting Protein Kinase 31 (OsCIPK31) Is Involved in the Development of Panicle Apical Spikelets
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Peng, Yongbin, primary, Hou, Feixue, additional, Bai, Que, additional, Xu, Peizhou, additional, Liao, Yongxiang, additional, Zhang, Hongyu, additional, Gu, Chaojian, additional, Deng, Xiaoshu, additional, Wu, Tingkai, additional, Chen, Xiaoqiong, additional, Ali, Asif, additional, and Wu, Xianjun, additional
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- 2018
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11. Mutation in Rice Abscisic Acid2 Results in Cell Death, Enhanced Disease-Resistance, Altered Seed Dormancy and Development
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Liao, Yongxiang, primary, Bai, Que, additional, Xu, Peizhou, additional, Wu, Tingkai, additional, Guo, Daiming, additional, Peng, Yongbin, additional, Zhang, Hongyu, additional, Deng, Xiaoshu, additional, Chen, Xiaoqiong, additional, Luo, Ming, additional, Ali, Asif, additional, Wang, Wenming, additional, and Wu, Xianjun, additional
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- 2018
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12. Identification and gene mapping of a grain-filling mutant gef1 in rice
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DU, KangXi, primary, JIANG, QingShan, additional, XU, PeiZhou, additional, HAN, BaoLin, additional, PENG, YongBin, additional, FU, Yao, additional, WU, TingKai, additional, ZHAO, DeMing, additional, CHEN, JiaBin, additional, CHEN, XiaoQiong, additional, ZHANG, HongYu, additional, and WU, XianJun, additional
- Published
- 2016
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