Search

Your search keyword '"Pierre Galka"' showing total 2 results
Start Over Author "Pierre Galka" Publication Year Range Last 10 years
2 results on '"Pierre Galka"'

1. QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides.

Author

Pierre Galka, Elisabeth Jamez, Gilles Joachim, and Patrice Soumillion

Subjects
Medicine, Science
Abstract

Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.

Published
2017
Full Text
View/download PDF

2. QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides

Author

Patrice Soumillion, Gilles Joachim, Pierre Galka, Elisabeth Jamez, and UCL - SST/ISV - Institut des sciences de la vie

Subjects
0301 basic medicine, Computer science, Oligonucleotides, lcsh:Medicine, Artificial Gene Amplification and Extension, Bioinformatics, Biochemistry, Polymerases, Polymerase Chain Reaction, Synthetic biology, chemistry.chemical_compound, Plasmid, DNA libraries, Cloning, Molecular, lcsh:Science, Polymerase, Synthetic Plasmids, Multidisciplinary, biology, Cloning (programming), Nucleotides, Genomics, Nucleic acids, Oligodeoxyribonucleotides, Engineering and Technology, Synthetic Biology, Research Article, Plasmids, Computational biology, Molecular cloning, DNA polymerase, Research and Analysis Methods, 03 medical and health sciences, Peptide Library, DNA-binding proteins, Genetics, Escherichia coli, Peptide library, Molecular Biology Techniques, Molecular Biology, Sequence Assembly Tools, 030102 biochemistry & molecular biology, Oligonucleotide, lcsh:R, Biology and Life Sciences, Computational Biology, Proteins, DNA, Synthetic Genomics, Genome Analysis, 030104 developmental biology, chemistry, biology.protein, lcsh:Q, Cloning
Abstract

Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.

Published
2017

Catalog

Books, media, physical & digital resources
See catalog results