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An increase in the number of drug-resistant microbes is a major threat to human health. Bacterial drug resistance is mostly mediated by biofilm formation. In this study, the culture filtrate from the edible mushroom, Pleurotus ostreatus , was fractionated to isolate compounds that inhibit the biofilm formation of six pathogenic bacteria. Notably, we isolated compounds 1-6 using bioassay-guided chromatographic separations. Spectroscopic and X-ray diffraction analyses identified 1 as a novel fused bicyclic pyrone-furan, named pleuropyronine, whereas 2-6 were known polyketides. Pleuropyronine inhibited biofilm formation in four Gram-negative bacteria, with IC50 values ranging from 5.4 to 8.7 µg/mL, whereas 2-6 exhibited IC50 values between 1.0 and 5.3 µg/mL against five bacteria. Additionally, pleuropyronine bioactivity was confirmed by the inhibition of exopolysaccharide and biofilm formation induced by C6-homoserine lactone. Thus, this may serve as a pioneering study on the pharmacological potential of isolated compounds, offering valuable insights for future research. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study evaluated the impacts of various drying techniques—hot-air drying (HD), microwave rolling-bed drying (MRD), hot-air microwave rolling-bed drying (HMRD), microwave vacuum rolling-bed drying (MVRD), and vacuum freeze drying (VFD)—on the drying efficiency, physicochemical qualities, and flavor profile of Pleurotus eryngii. The Page and Midilli models effectively predicted the drying kinetics. MRD and HMRD notably reduced drying time by 52.67 and 65.65%, respectively, compared to HD. MVRD and VFD were superior in preserving nutritional quality, achieving higher rehydration capacity, nutrient retention, and antioxidant capacity. MVRD, in particular, demonstrated the shortest drying time (116.67 min), minimal color change, and a crispy texture, attributed to its significant porosity (84.32%). In addition, MVRD-treated products were rich in flavor with higher concentrations of alcohols, aldehyde compounds, and monosodium glutamate–like taste. These findings suggest MVRD as an advantageous method for producing high-quality dried P. eryngii products, balancing efficiency with sensory and nutritional integrity. [ABSTRACT FROM AUTHOR]

Corybas carsei is one of New Zealand's most endangered orchids, with a single remaining population in the Whangamarino Wetland (North Island). Although orchid mycorrhizae have proved a critical aspect of orchid conservation globally, until now mycorrhizal fungi of C. carsei have not been identified or utilised for conservation. This study investigated fungal associates of C. carsei using three culture-based approaches: fungi grown directly from fragments of root tissue, from pelotons isolated from root-collars, and directly from macerated protocorm tissue. Fungi were cultured and identified as a diverse array of fungi through Sanger sequencing of the internal transcribed spacer region. This included the fungus Pleurotus purpureo-olivaceus (Basidiomycota; Agaricales), which belongs to a genus containing putative orchid mycorrhizal fungi, and the ericoid mycorrhizal fungus Oidiodendron eucalypti (Ascomycota; Leotiomycetes incertae sedis). No Rhizoctonia-like fungi, typical of orchid mycorrhizae, were found through any approach and fungi cultured in the present study likely represent endophytes, rather than mycorrhizal fungi. The potential of C. carsei having unculturable mycorrhizal associations, and the challenge this presents to conservation efforts, is discussed. [ABSTRACT FROM AUTHOR]

Utilizing agricultural waste to produce mushrooms may be a cost-effective and environmentally friendly proposition to address the nutritional and health demands of the growing global population. Mushrooms can grow on a range of substrates and their selection is based on their availability and cost. In this study, five types of local waste were mixed: olive crop residues (OC), coffee residue (CR) or rice husk (RH) with wheat straw (WS) and beech wood shavings (BW), respectively. Then, the mixtures were sprayed with 20% w/w lipid fermentation wastewater (LFW) from Rodosporidium toruloides that was used as an alternative substrate-moistening method. Afterwards, these mixtures were tested for cultivating Pleurotus spp., Ganoderma spp. and Lentinula edodes. The results showed that the substrate significantly affected the incubation period and the biological efficiency (BE), with OC mixed substrates proving to be the most favorable across the different species. Pleurotus spp. had the shortest cultivation times and the highest BE, while G. lucidum required the longest incubation periods and had the lowest BE, particularly on CR substrates. The study also found that substrates affected mushroom morphology. Nutritional analysis revealed significant differences in protein, polysaccharides, lipids, ash and energy content, depending on the species and substrate. High protein levels were found in P. eryngii (28.05–29.58% d.w.) and G. resinaceum (28.71–29.90% d.w.). The elevated total phenolic compounds (28.47–40.17 mgGAE/g) values in carposomes from CR and OC substrates for Ganoderma spp., L. edodes, P. pulmonarius and P. ostreatus, along with antioxidant activity (DPPH, ABTS, FRAP) assays, highlighted the crucial role of substrate composition in enhancing the medicinal properties of mushrooms. The mixed substrates also influenced the fatty acid (FA) and polysaccharide composition, with WS increasing unsaturated FAs and glucose (<69.8%) being the primary monosaccharide. The study suggests that using the spraying method of 20% w/w LFW as a moisture agent in these substrates is effective for mushroom production. [ABSTRACT FROM AUTHOR]

Pleurotus ostreatus, due to its saprophytic nature, can extract nutrients and bioactive compounds from the substrate on which it is grown. This study aimed to assess the effect of adding spent coffee grounds (SCG) and potato peel (PPW) in the wheat straw substrate formulation to grow over the production indicators, physicochemical, techno-functional, total chemical compounds, and antioxidant properties. Treatments were described as follows: T1, wheat straw at 100%; T2, wheat straw at 80% + 10% of SCG + 10% of PPW; T3, wheat straw at 70% + 15% of SCG + 15% of PPW; T4, wheat straw at 60% + 20% of SCG + 20% of PPW. After P. ostreatus growth, non-differences were found in production indicators for T1–T4, including biological efficiency, production rate, and yield. With respect to P. ostreatus dried powders, T1–T4 showed pH values near neutrality concerning soy protein (SP), and the color samples were beige. Also, T2 and T3 exert higher water-holding (WHC) values, while T1–T4 exert higher oil-holding (OHC) and emulsifying capacity (EC) values concerning SP, in dependence on the growth substrate. T1–T4 showed lower swelling (SC) and T1–T3 lower gelling capacity (GC) values. Regarding total chemical compounds and antioxidant properties of P. ostreatus extracts, growth substrate and solvent extraction have an effect on metabolite content and antiradical and reducing power properties. The multivariate analysis revealed that T2 water extracts exert the highest total tannin (TTC) and protocatechuic acid contents (PAC), as well as the highest antiradical (RCSA) and reducing power (RPA) values. In conclusion, this study demonstrated that using SCG and PPW as a partial substitute for substrate (what straw) enhances the physicochemical, techno-functional, and antioxidant activity of P. ostreatus. [ABSTRACT FROM AUTHOR]

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Green mould contamination causes a significant challenge to mushroom growers in Malaysia leading to reduced yields and economic losses in the widely cultivated and marketed edible grey oyster mushroom, Pleurotus pulmanorius. This study aimed to identify the causal agents of green mould contaminants and determine the critical points in the cultivation process in the farm that contribute to green mould contamination. Samples of mushroom substrate (sawdust), spawn substrate (corn), environmental sources and tools were collected at different stages of mushroom cultivation. As results, the causal agents of green mould contamination were identified as Trichoderma pleuroti , T. harzianum and T. ghanese. Prior to steam pasteurisation and after steam pasteurisation, the spawn substrate and mushroom substrate were found to be free of Trichoderma. However, Trichoderma was detected in water, air within the production house and on cleaning tools. This findings suggests that water could serve as the source of green mould introduction in mushroom farms, while cultivation practices such as watering and scratching during the harvesting cycle may contribute to adverse green mould. Understanding these critical points and causal agents provides information to mitigate the green mould contamination throughout the grey oyster mushroom cultivation process. [Display omitted] • Pleurotus mushroom cultivation in Malaysia faces contamination by green mould. • T. pleuroti is the main causal agent followed by T. harzianum and T. ghanense. • Water is the primary source followed by air inside the production house. • Watering is one of critical points in mushroom cultivation. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Novel substrates consisted of different fresh agro-industrial residues, their corresponding and commercial spent mushroom substrates (i.e. SMS deriving from laboratory-scale experiments and SMS deriving from industrial-scale experiments by Green Zin S.A. - SMS GZ) and Pleurotus waste (PW; stipes/mishappen mushrooms) were used in blends for a new cultivation cycle of Pleurotus ostreatus and P. eryngii mushrooms in bags. Their impact on the biochemical properties (intra-cellular polysaccharides - IPSs, protein, lipid, total phenolic compounds - TPCs, individual carbohydrates composition of the IPSs) in the first- and second-flush whole mushrooms, pilei and stipes, as well as the fatty acids composition, the antioxidant activity (in the first-flush mushroom parts) and glucan content of stipes were examined. Both species produced satisfactory IPSs quantities in all substrates (28.69–46.38 %, w/w) and significant protein amounts (18.37–26.80 %, w/w). The further SMS addition (80 %, w/w instead of 40 %, w/w) in the cultivation substrates affected positively the mushroom IPSs values, whereas the highest protein content was detected in mushroom's parts cultivated on substrates consisted of fresh agro-industrial residues. Mushroom's lipid content was affected differently by the various substrate combinations, with SMS presence resulting in mushrooms with a lower fat content than those produced in substrates with PW addition. Fresh substrates with PW and those with coffee residue were the most favorable for TPCs production. Regarding production flushes, the nutritional value of mushrooms was comparable between them, only a slight increase in TPCs of second-flush carposomes was detected. Glucose was the predominant monosaccharide of the produced IPSs, combined with a significant production of total and β- glucans. SMSs and PW addition had a positive impact on antioxidant activity, too. A higher quantity of lipids, TPCs and significant antioxidant activity were detected in all Pleurotus pilei than stipes, whereas the latter were richer in IPSs. Both pilei and stipes had a significant protein amount. Hence, the data obtained by this study support the positive effect of different types of SMS and mushroom waste on P. ostreatus and P. eryngii nutritional value.

Pleurotus pulmonarius (Pp), commonly called the Phoenix mushroom, is an edible tropical species with significant interest for its health-promoting properties. Renowned for its nutraceutical potential, Pp exhibits anti-inflammatory, anti-cancer, and prebiotic effects, positioning it as a valuable resource in functional food and therapeutic research. However, its anti-ulcer potential remains largely unexplored, prompting this investigation. The phytochemical profile and antibacterial activities of Pp were assessed using standard procedures. Thirty-five male Wistar rats were randomly assigned to five groups (n=7 per group): normal control (CN), untreated control (CU), pre-treated control with antacid (CP) and two groups treated with Pp supplements at 10% (FS10) and 30% (FS30) concentrations. Following 14 days of treatment, gastric ulcers were induced with indomethacin (40 mg/kg orally) after a 24-hour fast. Four hours post-ulcer induction, rats were anesthetized and stomachs were excised for analyses of ulcer score, nitrite, mucin, H2O2 and H+K⁺-ATPase activity. Stomach tissues were further examined through histopathological analysis. Data were analyzed using descriptive statistics and one-way ANOVA at α=0.05. The study confirmed the presence of all evaluated phytochemicals in Pp. Treatments with FS10 and FS30 resulted in ulcer inhibition rates of 64.5% and 56.58%, respectively. Significant improvements were observed in gastric parameters (total protein, nitric oxide, mucin content, H2O2 levels, malondialdehyde, and H⁺K⁺-ATPase) activities, in the FS10 and FS30 groups compared to the control groups (CN and CU). These findings underscore the antioxidant and gastroprotective properties of Pp, suggesting its potential as a natural therapeutic agent for managing gastric ulcers. [ABSTRACT FROM AUTHOR]

Parasitic gastrointestinal nematodes (GINs) cause diseases that are a major challenge to livestock production. Chemical control is the most widely used method, but it has many economic and environmental problems. This study was to evaluate in vitro the nematicidal and enzymatic activity of Pleurotus djamor against Trichostrongylus spp., and Strongyloides papillosus. The fungus was cultivated in a solid medium composed of wheat seeds for 25 days. The enzyme extraction was then performed using distilled water in the ratio of 1:5. Subsequently, by means of filtration and then centrifugation, the cell-free crude extract (CFCE) was obtained. Proteolytic activity was measured at pH 5 using the caseinolytic assay. The nematophagous assay of the P. djamor on the infective larvae (L3) was performed for 6 h. The nematicidal effect of the CFCE (active and denatured) was analysed over a period of 48 h. The CFCE showed proteolytic activity of 8.9 ± 0,5 U mL−1. P. djamor reduced the number of L3 by 75% (p < 0.01). The active CFCE showed a statistical difference compared to the control and the denatured (p < 0.01). On the other hand, there was no significant difference between the denatured group and the control group (without enzymes). The percentage reduction of L3 was 57%. This result suggests that the nematicidal action occurred mainly due to the activity of the proteases present in the CFCE. This study reports for the first time the applicability of proteases produced by P. djamor in the biochemical control of GINs, which could become an alternative biopesticide in the future. [ABSTRACT FROM AUTHOR]

Melanin plays an important role in protecting skin cells from harmful UV radiation, but its uneven pigmentation in the skin sometimes demands cosmetic resolution. As a safe and effective way of evening the skin tone, enzymatic decolorization of melanin at the stratum corneum has been proposed. In this regard, the use of lignin peroxidase, in the presence of hydrogen peroxide and veratryl alcohol, an electron mediator, has been explored. Here, we first show that versatile peroxidase (VP) and aryl alcohol oxidase (AAO) purified from the Pleurotus eryngii liquid culture can be used to decolorize melanin without exogenous hydrogen peroxide. This resembles the oxidative degradation pathway of lignin in nature; AAO generates hydrogen peroxide from veratryl alcohol, and VP utilizes generated hydrogen peroxide while using veratryl alcohol as a mediator. We further explored the use of POX_Pe, the crude peroxidase preparation of the Pleurotus eryngii liquid culture, for melanin decolorization because of its better stability. Using POX_Pe and veratryl alcohol, over 60 % of melanin decolorization was obtained in 1 h in the absence of exogenous hydrogen peroxide addition. Furthermore, POX_Pe could decolorize melanin in a 3D human pigmented epidermis model, demonstrating its possible applications in cosmetics. [Display omitted] • Enzymatic decolorization of the skin pigment, melanin, is drawing great attention. • Lignin degrading pathway of Pleurotus eryngii was re-purposed to decolorize melanin. • The system consists of versatile peroxidase and aryl alcohol oxidase. • The crude preparation of the enzymes showed benefits over purified enzymes. • High degree of melanin decolorization in the absence of toxic H 2 O 2 is described. [ABSTRACT FROM AUTHOR]

Based on the cultivation environment and material parameters of Pleurotus pulmonarius sticks, a P. pulmonarius stick cutting machine is designed to solve the problem of directly realizing the cutting of P. pulmonarius sticks for a net-shaped mushroom stick cultivation layer frame. The machine consists of three parts: a traveling trolley, an XYZ three-axis moving mechanism, and a clamping and cutting mechanism. Based on the force analysis of the clamping and cutting mechanism and the P. pulmonarius sticks, the key components were designed and selected. A prototype was constructed, and several cutting tests were conducted, with the cutting speed, number of cutting circles, and depth of the cut as the main factors. The success rate of cutting, the roundness of the cut, and the degree of loss in terms of the cutout substrate, were used as the performance indicators. An optimized design, based on orthogonal testing, was employed to determine the optimal parameter combinations for the cutting process. The optimal parameters were found to be a cutting speed of 376.3 r/min, 5.4 cutting circles, and a cutting depth of 4 mm. Finally, a validation test was carried out on the machine, and the results show that under the same parameter conditions, the success rate of cutting was 86.6%, the roundness of the cut was 0.235, and the degree of loss in terms of the cutout substrate was 0.851%, which is consistent with the optimization results. This study provides a reference, including a practical reference, for the design and development of a machine to realize the cutting of P. pulmonarius sticks for a cultivation layer frame. [ABSTRACT FROM AUTHOR]

The yield attributes and nutrient compositions of Pleurotus pulmonarius under four different substrates (maize-kernel, Empty Palm Fruit Bunch (EPFB), Dry Banana Leaf (DBL), and the Control) were studied in a humid tropical environment. The experimental design was a completely randomized design with nine replications. Variations in the morphological, proximate, vitamin, and phytochemical attributes of the mushrooms were recorded. The data were subjected to statistical analysis. The result revealed a significant substrate effect on the studied attributes. The DBL substrate significantly improved the pileus circumference (39.07 cm), pileus area (124.6 cm), length of stipe (6.37 cm), pileus diameter (12.43 cm), and moisture content in P. pulmonarius, while the maize-kernel substrate had significantly higher protein (4.6%) and flavonoids (14.42%) contents compared to other substrates. The substrate from EPFB gave significantly higher levels of vitamin B1 (3.8 mg/100 g), vitamin B2 (5.7 mg/100 g), vitamin C (114 mg/100 g), and vitamin E (17.4 mg/100 g) than the other substrates. The correlation results reveal that the concentrations of phytochemicals in P. pulmonarius tend to decrease as the morphological attributes expand. This substrate effect can be harnessed to produce mushrooms for specific needs. EPFB substrates can be used to enhance dietary supplementation and antioxidant activity in human nutrition. Mushrooms grown on the substrate formulated with maize kernel could serve as an alternative for animal protein because of their high protein content, while mushrooms produced on the DBL substrate with the highest yield in most attributes are recommended for commercial cultivation of P. pulmonarius. [ABSTRACT FROM AUTHOR]

The selenized modification of purified Pleurotus eryngii polysaccharides (PPEP) were carried out in a nitric acid-sodium selenite system, and the preliminary structural characterization of PPEP and selenized PPEP (613.87 μg/g of selenium) (SPPEP) were carried out by advanced instrumentation techniques. It was found that the selenization modification did not seriously affect the structure of the PPEP. However, the SPPEP exhibited a lower molecular weight, more glycosidic bonds, reduced crystallinity, and improved thermal stability. Through in vivo animal experiments, the study found that PPEP and SPPEP were non-toxic to macrophages. Furthermore, they markedly increased the thymus and spleen indices of immunocompromised mice and improved the histomorphology of these organs. Moreover, PPEP and SPPEP enhanced the mice's immune ability by markedly increasing the level of immune factor secretion, bone marrow hematopoietic function, and macrophage phagocytic capacity. In addition, the immune activity of SPPEP was better than PPEP's activity. [Display omitted] • The polysaccharide of P.eryngii is selenized for the first time. • The selenization modification did not seriously affect the structure of the PPEP. • The immune activity of SPPEP is better than PPEP's activity. • Selenide polysaccharides were evaluated for non-toxicity. [ABSTRACT FROM AUTHOR]

Novel substrates consisted of different fresh agro-industrial residues, their corresponding and commercial spent mushroom substrates (i.e. SMS deriving from laboratory-scale experiments and SMS deriving from industrialscale experiments by Green Zin S.A. - SMS GZ) and Pleurotus waste (PW; stipes/mishappen mushrooms) were used in blends for a new cultivation cycle of Pleurotus ostreatus and P. eryngii mushrooms in bags. Their impact on the biochemical properties (intra-cellular polysaccharides - IPSs, protein, lipid, total phenolic compounds - TPCs, individual carbohydrates composition of the IPSs) in the first- and second-flush whole mushrooms, pilei and stipes, as well as the fatty acids composition, the antioxidant activity (in the first-flush mushroom parts) and glucan content of stipes were examined. Both species produced satisfactory IPSs quantities in all substrates (28.69-46.38 %, w/w) and significant protein amounts (18.37-26.80 %, w/w). The further SMS addition (80 %, w/w instead of 40 %, w/w) in the cultivation substrates affected positively the mushroom IPSs values, whereas the highest protein content was detected in mushroom's parts cultivated on substrates consisted of fresh agroindustrial residues. Mushroom's lipid content was affected differently by the various substrate combinations, with SMS presence resulting in mushrooms with a lower fat content than those produced in substrates with PW addition. Fresh substrates with PW and those with coffee residue were the most favorable for TPCs production. Regarding production flushes, the nutritional value of mushrooms was comparable between them, only a slight increase in TPCs of second-flush carposomes was detected. Glucose was the predominant monosaccharide of the produced IPSs, combined with a significant production of total and ß- glucans. SMSs and PW addition had a positive impact on antioxidant activity, too. A higher quantity of lipids, TPCs and significant antioxidant activity were detected in all Pleurotus pilei than stipes, whereas the latter were richer in IPSs. Both pilei and stipes had a significant protein amount. Hence, the data obtained by this study support the positive effect of different types of SMS and mushroom waste on P. ostreatus and P. eryngii nutritional value. [ABSTRACT FROM AUTHOR]

The antennal sensilla play an important role in many behavioral activities of insects. The fungivorous beetle Triplax ainonia Lewis (Erotylidae) is an important pest which prefers to feed on Pleurotus mushrooms. In order to clarify the types, number, and distribution of the antennal sensilla of male and female T. ainonia, scanning electron microscopy was used. The results showed that there were five sensillum types on the antennae of adults male and female, including Böhm's bristles (BB), sensilla chaetica (three subtypes: SC 1, SC 2, and SC 3), sensilla basiconica (three subtypes: SB 1, SB 2, and SB 3), sensilla trichodea (ST), and sensilla styloconica (SS). Among all the sensilla, the number of SB 2 was the most abundant in both sexes. We found that there was no sexually dimorphic in the sensillum types, but there were differences in the number, lengths, and diameters of some sensilla between males and females. Based on the information of the morphology and distribution of the sensilla, the potential functions of the antennal sensilla of T. ainonia adults were discussed. The results of this study provide a basis for further study on the behavioral ecology and electrophysiology of the fungivore beetles of the Erotylidae. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study investigated the impact of Lactiplantibacillus plantarum PC-004 (PL) and Kazachstania bulderi PY-1 (PY), selected from home-made Paocai, on the physicochemical properties and volatile flavor compounds of fermented Pleurotus eryngii. P. eryngii, fermented by PY, was maintained at about pH 5 throughout fermentation, while co-fermentation exhibited a higher acid production rate and lower post-acidification value compared to mono-fermentation by PL. The viable cell counts of PY exhibited no difference between the mono- and co-fermentation, while PL significantly decreased in the co-fermentation after 168 h. Compared to mono-fermented P. eryngii, co-fermentation had lower levels of certain biogenic amines. Co-fermented P. eryngii had a higher value of nitrite in the early fermentation stage, but this decreased during mono-fermentations. Additionally, among the three different groups, PY fermentation had more esters, PL fermentation had more ketones and aldehydes, and co-fermentation had more abundance and diverse volatile flavor compounds than mono-fermentations. Our results suggest that co-fermentation with K. bulderi and L. plantarum is suitable for producing fermented P. eryngii, highlighting their potential for the fermentation of edible mushrooms. [ABSTRACT FROM AUTHOR]

The oyster mushroom is cultivated globally, renowned for its unique texture and umami flavor, as well as its rich content of nutrients and functional ingredients. This study aims to identify the descriptive sensory characteristics, assess the consumer acceptability of new superior lines and cultivars of yellow oyster mushrooms, in addition to exploring the relationship between these descriptive characteristics and consumer acceptability. Statistical analyses were performed using one-way analysis of variance (ANOVA), principal component analysis (PCA), and partial least squares regression (PLSR). Twenty attributes were delineated, including three related to appearance/color (gray, yellow, and white), four associated with the smell/odor of fresh mushroom (oyster mushroom, woody, fishy, and seafood smells), three pertaining to the smell/odor of cooked mushrooms (mushroom, umami, and savory smells), four describing flavor/taste (sweet, salty, umami, and savory tastes), and five for texture/mouthfeel (chewy, smooth, hard, squishy, and slippery textures). Consumer acceptability tests involved 100 consumers who evaluated overall liking, appearance, overall taste, sweetness, texture, savory taste, MSG taste, smell, color, purchase intention, and recommendation. The general oyster mushroom (548 samples) scored highest in acceptability. Seven attributes, namely fresh mushroom smell, seafood smell (fresh), fishy smell (fresh), umami smell (cooked), nutty smell (cooked), salty taste, and MSG taste with the exception of appearance showed significant differences among samples (p < 0.001). The three yellow oyster mushroom samples were strongly associated with attributes like hardness, softness (texture), sweet taste (745 samples), MSG taste, salty taste, squishy texture, and fishy smell (483 and 629 samples). The development of sensory lexicons and increasing consumer acceptance of new superior lines and cultivars of yellow oyster mushroom will likely enhance sensory quality and expand the consumer market, aligning with consumer needs and preferences. [ABSTRACT FROM AUTHOR]

This research investigates the growth performance of Clarias batrachus when supplemented with Pleurotus florida freeze-dried extract (PFME) over 60 days. Triplicate groups of fish were fed experimental diets containing varying concentrations of PFME (0.5%, 1%, 1.5% and 2%), each adjusted to 5% of their wet body weight biweekly. Results demonstrate that PFME-enriched diets significantly enhanced the growth metrics of Clarias batrachus compared to a control group fed a standard diet. Fish fed with Diet III, containing 1.5% PFME, exhibited the highest weight gain of 31.98 ± 0.45 grams, followed by Diet IV (26.59 ± 0.33 grams), Diet II (25.26 ± 0.49 grams), Diet I (23.90 ± 0.30 grams), and the control group (17.78 ± 0.61 grams). This trend in weight gain correlates with biweekly measurements showing accelerated growth, particularly evident after the first month of feeding. Length measurements also reflected enhanced growth rates, with Diet III yielding the longest fish at 21.24 ± 0.38 cm. Furthermore, specific growth rates were notably higher in fish-fed Diet III (2.11 ± 0.05%), indicating superior growth potential compared to other diets and the control. Feed conversion ratios (FCR) were comparable across experimental diets, suggesting efficient feed utilization, particularly evident in Diet III (FCR 1.55 ± 0.45), which required less feed to produce more fish biomass. Moreover, PFME supplementation positively influenced the feeding rates and survival rates of Clarias batrachus. Fish on Diet III exhibited a consistent feeding rate (3.01 ± 0.06%) similar to other experimental diets, while achieving a high survival rate of 100 ± 0.02%, signifying improved health and immunity. Pleurotus florida extract, particularly at 1.5% concentration (Diet III), proves effective in enhancing the growth performance of Clarias batrachus. This study underscores PFME's potential as a beneficial dietary supplement in the aquaculture, promoting efficient growth and potentially reducing production costs. Further research into the physiological mechanisms underlying these effects could provide deeper insights into optimizing fish nutrition and health in aquaculture settings. [ABSTRACT FROM AUTHOR]

Pleurotus citrinopileatus Singer (PCS) has attracted increasing attention as a raw material for medicine and food. Its quality is greatly affected by the accumulation of metabolites, which varies with the applied drying methods. In this study, we utilize an approach based on ultra-high-performance liquid chromatography/Q Exactive mass spectrometry (UHPLC-QE-MS) to reveal the metabolic profiles of PCS from three different drying methods (natural air-drying, NAD; hot-air-drying, HAD; vacuum freeze-drying, VFD). The results showed that lipids, amino acids and their derivatives were all important secondary metabolites produced during NAD, HAD and VFD treatments, with the key differential metabolites of PCS during drying including fifteen lipids and seven amino acids. Meanwhile, VFD was the best way for long-term preservation of dried PCS. Hot-drying methods, especially HAD, can improve the medicinal component of PCS. Furthermore, KEGG enrichment analysis highlighted 16 pathways and indicated that amino acid metabolism might be the key metabolite pathway for the PCS drying process. Our study elucidates the relationship between drying methods and metabolites or metabolic pathways of PCS to determine the mechanisms affecting the quality of PCS, and finally provides reference values for further development and application in functional food and medications. [ABSTRACT FROM AUTHOR]

Summary: Pleurotus pulmonarius is one of the most widely consumed mushrooms containing low calories and fat. This study aimed to extract polysaccharides mainly ß‐glucan from P. pulmonarius by‐products including cap and stem using pulsed electric field (PEF) and study their prebiotic properties. The by‐products were subjected to PEF at a field strength of 5, 7.5 and 10 kV cm−1, a frequency of 15 Hz and a number of pulses of 12 000; then, the ß‐glucan content was determined with K‐YBGL assay test kit compared with a conventional extraction method using hot water at 100 °C for 6 h. In raw materials, crude carbohydrate contents in the cap and stem were 50.42% and 68.78%, respectively, and the ß‐glucan contents were 34.03% and 50.22%, respectively. After treated with PEF at 7.5 kV cm−1, the highest yield of 4.18% with ß‐glucan content of 14.10% was achieved. And the stem treated at 10 kV cm−1 showed the highest yield of 2.34% with ß‐glucan content of 21.39%. Comparatively lower yield and ß‐glucan contents were found in the samples treated with hot water. Scanning electron micrographs suggested that PEF caused severe damage to both samples. Additionally, the study of the prebiotic properties revealed that both products exhibited the ability to stimulate the growth of target lactic acid bacteria and the production of short‐chain fatty acid. This study suggested that PEF could enhance the extraction efficiency of β‐glucan with prebiotic potential from P. pulmonarius by‐products. [ABSTRACT FROM AUTHOR]

Summary: The preservation of King oyster mushrooms (KOMs) during storage remains a significant challenge due to their susceptibility to microbial spoilage, browning and quality degradation. This study explores the efficacy of electron beam irradiation (EBI) as a novel preservation technique for KOMs, targeting these specific storage issues. Employing a range of detection techniques, we evaluated the impact of EBI on microbial contamination, essential qualities and the browning of KOMs. Our findings reveal that EBI significantly reduces microbial contamination, including total aerobic bacteria and Pseudomonas spp., effectively inhibiting pathogenic spoilage and the formation of browning lesions. The treatment successfully preserved the moisture content, water activity and minimised weight loss, thus maintaining the mushrooms' textural and visual quality. Notably, doses of EBI up to 2 kGy did not adversely affect the firmness of KOMs. Additionally, we determined that photostimulated luminescence and thermoluminescence are effective methods for detecting EBI treatment in KOMs, whereas electron spin resonance proved unsuitable due to the lack of a distinctive irradiation signal in high‐moisture content foods. These findings contribute to the development of an efficient preservation technique for perishable KOMs, offering potential applicability in the food industry. [ABSTRACT FROM AUTHOR]

A specific feature of mushrooms (including those of the genus Pleurotus) is their natural ability to absorb and accumulate many chemical substances present in their immediate environment, which makes them an excellent natural sorption material. Hence, fruiting bodies of mushrooms have been recognized for years as excellent indicators of the environment, reflecting its current state. Nevertheless, mushrooms can accumulate both health-promoting substances, such as bioelements, and toxic substances, such as heavy metals and organic compounds, including bisphenol A® (BPA). This organic chemical compound in the phenol group, although it has been withdrawn in the EU since 2010, is widely present in the environment around us. In the present experiment, we aimed to determine the effect of adding BPA to liquid media for in vitro cultures of Pleurotus spp. The biomass increases were determined. Moreover, the degrees of adsorption and desorption of BPA from the obtained freeze-dried biomass in two different environments (neutral and acidic) were determined as a function of time. This is the first study to determine the bioavailability of adsorbed BPA in obtained biomass by extracting the mycelium into artificial digestive juices in a model digestive system. BPA was added to the liquid Oddoux medium in the following amounts: 0.01, 0.5, and 0.5 g/250 mL of medium. The amounts of adsorbed and desorbed BPA were determined by flow injection analysis (FIA) with amperometric detection. The addition of BPA to the substrate reduced the biomass growth in each of the discussed cases. BPA adsorption by the mycelium occurred at over 90% and depended on the morphology of the mushroom (structure, surface development, and pore size). BPA desorption depended on the pH of the environment and the desorption time. Mushrooms are an excellent natural remedial material, but BPA is extracted into artificial digestive juices; therefore, consuming mushrooms from industrialized areas may have health consequences for our bodies. [ABSTRACT FROM AUTHOR]

Thailand is one of the major agricultural production countries in the world. Therefore, large amounts of agricultural waste are generated as by-products of the agroindustry. The wastes are usually discarded or burnt, resulting in environmental pollution. The main goal of this research was to evaluate the use of agricultural waste for mushroom cultivation. A total of 15 mushroom isolates were recovered from mother spawns and basidiocarps. They were screened for their cellulolytic enzyme activity on Czapek agar using carboxymethyl cellulose (CMC) as the sole carbon source. Two isolates of the oyster mushrooms, Pleurotus pulmonarius PP6 and Pleurotus ostreatus PO3, produced the best enzyme activities. To observe the mycelial growth on agricultural waste, the two oyster mushroom candidates were cultured in jars containing five different types of agricultural waste: corn husk, rice straw, coconut meal, coconut husk and sugarcane bagasse, and the jars were incubated at 25°C for six weeks. The results show that both isolates grew best on coconut meal, producing very densely packed mycelia. Meanwhile, corn husk and rice straw were also good sources for oyster mushroom cultivation. This study shows that these three substrates have the potential to be utilized in mushroom cultivation on a commercial scale. [ABSTRACT FROM AUTHOR]

Mycoviruses are usually transmitted horizontally via hyphal anastomosis and vertically through sporulation in natural settings. Oyster mushroom spherical virus (OMSV) is a mycovirus that infects Pleurotus ostreatus, with horizontal transmission via hyphal anastomosis. However, whether OMSV can be vertically transmitted is unclear. This study aimed to investigate the transmission characteristics of OMSV to progeny via basidiospores and horizontally to a new host. A total of 37 single-basidiospore offspring were obtained from OMSV-infected P. ostreatus and Pleurotus pulmonarius for Western blot detection of OMSV. The OMSV-carrying rate among monokaryotic isolates was 19% in P. ostreatus and 44% in P. pulmonarius. Then, OMSV-free and OMSV-infected monokaryotic isolates were selected for hybridization with harvested dikaryotic progeny strains. Western blot analyses of the offspring revealed that the OMSV transmission efficiency was 50% in P. ostreatus and 75% in P. pulmonarius, indicating vertical transmission via sexual basidiospores. Furthermore, we observed the horizontal transfer of OMSV from P. pulmonarius to Pleurotus floridanus. OMSV infection in P. floridanus resulted in significant inhibition of mycelial growth and yield loss. This study was novel in reporting the vertical transmission of OMSV through basidiospores, and its infection and pathogenicity in a new host P. floridanus. [ABSTRACT FROM AUTHOR]

Biological control using edible mushrooms as natural enemies is a sustainable alternative for pest management. Despite the well-established literature on toxins and secondary metabolites produced by these fungi in the biochemical control of nematodes, the nematicidal activity of proteases from different Pleurotus species is yet to be investigated. Therefore, this study aimed to correlate protease to the nematicidal activity of different mushrooms, Pleurotus sp., P. ostreatus (SB), P. ostreatus (Pearl), and P. djamor. For such a purpose, we performed motility assays of Panagrellus sp. at different time intervals, 6, 12, and 24 h for each of the mushrooms. In addition, the protease activity was measured using different pH (5, 7, and 9) and fermentation time intervals (45 and 75 days). Furthermore, we also evaluated the effect of this cell-free extract on Panagrellus sp. In response to these experiments, all edible mushrooms showed a reduction over 82% for the nematode-feeding activity (p < 0.01). The cell-free crude extract of each of the fungi studied showed nematocidal activity (p < 0.01). For the 45-day fermentation, P. djamor exhibited statistical significance (p < 0.01) compared with the others, reaching a reduction percentage of 73%. For the 75-day fermentation, Pleurotus sp. and P. ostreatus (Pearl) showed significant differences compared with the other fungi (p < 0.01), with reduction percentages of 64 and 62%, respectively. Herein, protease activity was associated with the nematicidal action of different Pleurotus species in controlling Panagrellus sp. [ABSTRACT FROM AUTHOR]

Pulu Mandoti, a local red rice (Oryza sativa L.) variety popular among Sulawesi residents, has gained recognition for its perceived health benefits, especially as a preferred dietary option for individuals with diabetes or those seeking to prevent obesity. Given the increasing consumption of mushrooms, particularly Pleurotus species, renowned for their nutritional and medicinal attributes, this study delves into the transformative effects of Pleurotus spp. fermentation on Pulu Mandoti, the indigenous rice variety. Proximate analysis disclosed elevated dry matter (91.99 ± 0.61%), crude protein (8.55 ± 0.15%), and crude fat (1.34 ± 0.05%) in Pleurotus cystidiosus fermentation compared to Pleurotus ostreatus and Pleurotus djamor. Concurrently, antioxidant and antidiabetic activities were notably improved in all Pleurotus fermentations. Pulu Mandoti fermented with P. cystidiosus outperformed other treatments, aligning with molecular docking results pinpointing 11-Eicosenoic acid, methyl ester, and butylated hydroxytoluene as optimal interactors with antioxidant receptors 5O0x and 2CKJ. Butylated hydroxytoluene demonstrated interactions with the antidiabetic receptor 2QV4, along with 9-Octadecenoic acid, methyl ester. These compounds, previously unreported in Pleurotus, displayed promising attributes as antioxidants and antidiabetic agents. Furthermore, the investigation delved into the fatty acid profiles, emphasizing the diverse range of potential bioactive compounds in fermented Pulu Mandoti. The findings of this research present a potential functional food rich in natural antioxidants and antidiabetic compounds, highlighting the yet undiscovered capabilities of Pleurotus spp. fermentation in augmenting the nutritional composition and bioactivity of indigenous rice varieties, specifically Pulu Mandoti. [ABSTRACT FROM AUTHOR]

Objective: To investigate the antioxidant activity of fermented tartaty buckwheat extract of Pleurotus eryngii in vitro as well as its protective effect on alcohol-induced liver and gastric mucosa injury in vivo. Methods: The study involved determining the contents of functional components in fermented tartary buckwheat extract and observing its antioxidant capacity. Mice models of chronic alcoholic liver and gastric mucosa injury were established using Lieber-DeCarli liquid feed. The protective effects of fermented tartary buckwheat extract at low and high doses (1.5 g/kg B.W., 3.0 g/kg B.W.) were investigated for both liver and gastric mucosa injury. Results: The extract of fermented buckwheat with Pleurotus eryngii contained more antioxidant components, the contents of polyphenols, flavonoids and triterpenes were 11.40±0.32 mg GAE/g DW, 17.19±0.30 mg RE/g DW and 7.59±0.24 mg/g, respectively. The contents of rutin and quercetin were as follows: 13.55±0.05 and 0.665±0.01 mg/g. The iron reducing antioxidant capacity and DPPH and ABTS+ free radical scavenging efficiency of Tartary buckwheat extract were 16.66±0.65, 33.49±1.26 and 15.68±1.17 µmol Trolox/g DW, respectively. Compared with the model group, both high-dose and low-dose groups significantly reduced malondialdehyde (P<0.05), aspartate aminotransferase (P<0.01), alanine aminotransferase (P<0.01), lactate dehydrogenase (P<0.05), and interleukin-1β (P<0.05) levels and significantly increased levels of superoxide dismutase (P<0.01) and glutathione peroxidase (P<0.01), downregulated protein expression levels of reactive oxygen species (P<0.01), rat sarcoma (P<0.01), rapidly accelerated fibrosarcoma (P<0.01), extracellular signal-regulated kinases (P<0.05), and mitogen activated protein kinase kinase (P<0.05). Conclusion: Fermented tartaty buckwheat extract of Pleurotus eryngii has good antioxidant activity, and has obvious protective effect on chronic alcoholic liver and gastric mucosa injury in mice. [ABSTRACT FROM AUTHOR]

In this study, the objective was to examine the utilization of the pyrolyzed by-products biomass as an adsorbent for extracting the dye Basic Red 46 (BR 46) from a solution. The pyrolyzed by-product containing Pleurotus mutilus biomass was characterized by thermogravimetric analysis (TGA), FTIR and µXRF. Different parameters effects such as, adsorbent dose, solution pH, contact time, temperature and initial dye concentration on the adsorption capacity of the pyrolyzed by-product biomass were examined. The solution was analyzed before and after the adsorption studies. With adsorbent dose of 1 g/L, contact time of 14 min, and solution pH of 7.5, the optimum yield of 88% was achieved. In order to fit the equilibrium data, the Langmuir, Freundlich, Temkin, and Khan isotherm models were used, and in order to fit the kinetics data, the pseudo-first-order, pseudo-second-order, Elovich, and Ritchie models were employed. Statistical analysis such as R2, RMSE, χ 2 and ARE was used to assess which model has the best fit with the experimental data. The results demonstrated that the experimental equilibrium data are well described by the Langmuir model, and the kinetic studies indicated that the adsorption of BR46 followed the Ritchie model. The greatest BR 46 adsorption capacity determined from experimental equilibrium data was around 135 mg/g at pH = 7.5, adsorbent dosage of 1 g/L. Moreover, thermodynamic analysis has demonstrated that the adsorption process was physical, exothermic, and spontaneous in nature. These results indicated that pyrolyzed by-product biomass might be used as a cheap material to extract textile colors out of aqueous effluents. [ABSTRACT FROM AUTHOR]

With the world's population rapidly increasing, the demand for high-quality protein is on the rise. Edible fungi breeding technology stands as a crucial avenue to obtain strains with high yield, high-quality protein, and robust stress resistance. To address the protein supply gap, Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, and spore hybridization techniques were employed to enhance Pleurotus djamor mycelium protein production. Beginning with the original strain Pleurotus djamor JD-1, ARTP was utilized to mutate spore suspension. The optimal treatment time for Pleurotus djamor spores, determined to achieve optimal mortality, was 240 s. Through primary and secondary screenings, 6 mutant strains out of 39 were selected, exhibiting improved protein yield and growth rates compared to the original strain. Among these mutagenic strains, 240S–4 showcased the highest performance, with a mycelial growth rate of 9.5±0.71 mm/d, a biomass of 21.45±0.54 g/L, a protein content of 28.75±0.92%, and a remarkable protein promotion rate of 128.03±7.29%. Additionally, employing spore hybridization and breeding, 7 single-nuclei strains were selected for pin-two hybridization, resulting in 21 hybrid strains. The biomass and protein content of 9 hybrid strains surpassed those of the original strains. One hybrid strain, H-5, exhibited remarkable mycelial protein production, boasting a mycelial growth rate of 26.5±0.7 mm/d, a biomass of 21.70±0.46 g/L, a protein content of 28.44±0.22%, and a protein promotion rate of 128.02±1.73%. Notably, both strains demonstrated about a 28% higher mycelial protein yield than the original strains, indicating comparable effectiveness between hybrid breeding and mutagenesis breeding. Finally, we analyzed the original and selected strains by molecular biological identification, which further proved the effectiveness of the breeding method. These findings present novel insights and serve as a reference for enhancing edible fungi breeding, offering promising avenues to meet the escalating protein demand. [Display omitted] • The growth rate of the ARTP mutant strain was better than original strain. • The hybrid strains after spore hybridization had higher protein yield. • ARTP mutagenesis and spore mutagenesis can effectively promote the growth of mycelia. • Molecular biological identification confirmed the effectiveness of the breeding method. • The two methods can be used to breed new strains effectively. [ABSTRACT FROM AUTHOR]

This research investigated the antioxidant responses of Pleurotus florida at different concentrations of gas oil [0% (control), 2.5%, 5%, and 10% (v:v)] for 30 days. The activities of superoxide dismutase and catalase enzymes decreased in responses to the gas oil presence by an average of 83% and 49%, respectively. In contrast, the activities of the ascorbate peroxidase and glutathione peroxidase enzymes displayed an upward trend in the groups cultured in oil-contaminated media. The gas oil contaminant increased total phenol and flavonoid accumulation, reflecting the variation in secondary metabolism. According to the 1,2-diphenyl-2-picrylhydrazyl radical scavenging, the 2.5% gas oil treatment resulted in the highest antioxidant activity (48 μg mL−1). The highest scavenging activity of nitric oxide radicals (IC50 = 272 μg mL−1) was observed in the treatment with the highest gas oil concentration (10%). Also, this treatment showed an excellent ability to chelate Fe+2 ions (IC50 = 205 μg mL−1). The IC50 values of methanolic extract for nitric oxide scavenging activity and metal chelating ability were significantly reduced by increasing gas oil concentration in the treatments. With increasing the gas oil concentration, malondialdehyde content as a criterion measure of lipid peroxidation level showed significant reduction. These results show that P. florida is resistant to and a compatible mushroom with oil pollutants. Also, the activity of glutathione peroxidase and the ascorbate-glutathione cycle detoxify nitric oxide radicals and products of reactive oxygen species–induced lipid peroxidation in the gas oil treatments. [ABSTRACT FROM AUTHOR]

Ginkgo biloba is widely planted as a colorful foliage tree, and its leaf can be used as a biomass energy source, but it has been underutilized for a long time. The aim of this study was to investigate the potential of garden waste as a substrate component in the cultivation process of the king oyster mushroom (Pleurotus eryngii), with the goal of enhancing both the yield of P. eryngii and the efficiency of energy use. The percentages of G. biloba leaf powder in the substrate were 10.5% and 21% to replace sawdust or sugarcane bagasse in a typical substrate. A substrate formulation that could completely replace sawdust and sugarcane bagasse was selected by analyzing mycelial growth rate, days of production, fruiting body length, biological efficiency, yield, stipe thickness, pileus diameter and laccase activity. The results showed that Y1 (treatment with 21% G. biloba leaf powder and sugarcane bagasse) had the highest yield (303.1 ± 31.9 g), which was higher than that of CK (control) (259.3 ± 37.4 g). The crude fiber content of the samples grown on substrate Y1 (as 7.43%) was higher than CK (7.37%). In addition, P. eryngii grown on substrate Y1 had the highest laccase activity for the complete colonization of the mycelium. Thus, these findings suggest that G. biloba leaf powder represents a viable and economical supplement for enhancing both the yield and quality of P. eryngii. [ABSTRACT FROM AUTHOR]

Due to the increasing populations of anthelmintic-resistant gastrointestinal nematodes and as a consequence of the adverse effects of synthetic drugs, this study focuses on the search for secondary metabolites with nematocidal activity from the edible mushroom Pleurotus djamor using The proton nuclear magnetic resonance (1H-NMR) metabolomics. The highest activity was shown by the ethyl acetate fractions of mycelium (EC50 290.8 µg/mL) and basidiomes (EC50 282.7 µg/mL). Principal component analysis (PCA) and hierarchical data analysis (HCA) of the 1H-NMR metabolic profiles data showed that the ethanolic extracts, the ethyl acetate, butanol, and water fractions from mycelium have different metabolic profiles than those from basidiomes, while low polarity (hexane) fractions from both stages of fungal development show similar profiles. Orthogonal partial least squares discriminant analysis (OPLS-DA) allowed the identification of signals in the 1H-NMR metabolic profile associated with nematocidal activity. The signals yielded via OPLS-DA and bidimensional NMR analysis allowed the identification of uracil as a component in the ethyl acetate fraction from basidiomes, with an EC50 of 237.7 µg/mL. The results obtained showed that chemometric analyses of the 1H-NMR metabolic profiles represent a viable strategy for the identification of bioactive compounds from samples with complex chemical profiles. [ABSTRACT FROM AUTHOR]

Edible grey oyster mushroom, Pleurotus sajor-caju, β (1,3), (1,6) glucan possesses a wide range of biological activities, including anti-inflammation, anti-microorganism and antioxidant. However, its biological activity is limited by low water solubility resulting from its high molecular weight. Our previous study demonstrated that enzymatic hydrolysis of grey oyster mushroom β-glucan using Hevea β-1,3-glucanase isozymes obtains a lower molecular weight and higher water solubility, Pleurotus sajor-caju glucanoligosaccharide (Ps-GOS). Additionally, Ps-GOS potentially reduces osteoporosis by enhancing osteoblast–bone formation, whereas its effect on osteoclast–bone resorption remains unknown. Therefore, our study investigated the modulatory activities and underlying mechanism of Ps-GOS on Receptor activator of nuclear factor kappa-Β ligand (RANKL) -induced osteoclastogenesis in pre-osteoclastic RAW 264.7 cells. Cell cytotoxicity of Ps-GOS on RAW 264.7 cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and its effect on osteoclast differentiation was determined by tartrate-resistant acid phosphatase (TRAP) staining. Additionally, its effect on osteoclast bone-resorptive ability was detected by pit formation assay. The osteoclastogenic-related factors were assessed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), Western blot and immunofluorescence. The results revealed that Ps-GOS was non-toxic and significantly suppressed the formation of mature osteoclast multinucleated cells and their resorption activity by reducing the number of TRAP-positive cells and pit formation areas in a dose-dependent manner. Additionally, Ps-GOS attenuated the nuclear factor kappa light chain-enhancer of activated B cells' P65 (NFκB-P65) expression and their subsequent master osteoclast modulators, including nuclear factor of activated T cell c1 (NFATc1) and Fos proto-oncogene (cFOS) via the NF-κB pathway. Furthermore, Ps-GOS markedly inhibited RANK expression, which serves as an initial transmitter of many osteoclastogenesis-related cascades and inhibited proteolytic enzymes, including TRAP, matrix metallopeptidase 9 (MMP-9) and cathepsin K (CTK). These findings indicate that Ps-GOS could potentially be beneficial as an effective natural agent for bone metabolic disease. [ABSTRACT FROM AUTHOR]

Anti-proliferative and antioxidant activities of Pleurotus pulmonarius and P. citrinopileatus water-soluble polysaccharides were examined using human colon adenocarcinoma (HT29) and human normal colonic epithelial (CCD841 CoTr) cell lines in vitro. Polysaccharides were prepared by cold and hot water extraction methods and chemically characterized. The monosaccharide analysis showed that all the preparations contained hexoses--mannose, glucose, galactose, and 3-O-methyl-hexose (identified as 3-O-methyl-galactose)--as well as trace amounts of pentose identified as arabinose. Linkage analysis, on the other hand, revealed a wide variety of monosaccharide linking bonds in the studied polysaccharides, depending on the polymer source and the method of isolation. Both fungal polysaccharide preparations with increasing concentrations showed lower DPPH free radical reducing activity. The degree of reduction depended on the origin of the compounds and the method of their preparation. Stimulation of nitric oxide (NOx) production by the tested cells was observed after their incubation with the polysaccharides. The tested compounds at lower concentrations (up to 500 µg/mL) did not significantly influence the metabolic activity of the tumor and normal cells after 24 h of incubation. The viability of the tumor and normal cells was limited after the impact of the polysaccharides in the concentration range of 25-1000 µg/mL. The hot and cold water isolated polysaccharides did not induce changes in the normal and tumor cell cytoskeleton organization. The paper presents newly prepared and characterized hot and cold isolated water-soluble polysaccharides with promising pro-health potential against tumor and normal human colon epithelium cells. [ABSTRACT FROM AUTHOR]

Heavy metal contamination has emerged as a global environmental concern, with tannery effluents serving as a significant source of these pollutants. The discharge of tannery effluents (TEs) into natural ecosystems has given rise to a spectrum of catastrophic risks, exacerbating concerns related to public health, safety, and environmental integrity. This current study focuses on the mycoremediation of the heavy metals present in TE, employing the mycelia of Pleurotus opuntiae, an environmentally sustainable solution. The toxicity of TE was rigorously characterized by evaluating a range of physicochemical parameters in accordance with the American Standard and Testing Methods. Subsequently, various diluted concentrations of effluent (25%, 50%, 75% and 100%) were incorporated into MDA media to assess the tolerance index (TI) of P. opuntiae. Notably, the highest TI was observed in the 25% and 50% TE concentrations, while no growth was observed in the 75% and 100% groups due to the exceptionally elevated heavy metal content. P. opuntiae demonstrated remarkable efficacy in heavy metal removal, with the most substantial reductions recorded in the 25% diluted effluent (91.3% Pb, 72.2% Cr and 66.5% Zn), closely followed by the 50% diluted effluent. The highest intracellular bioaccumulation was observed for Pb (17.2 µg/g), outperforming Cr (14.5 µg/g) and Zn (8.5 µg/g) in mycelia grown in 25% diluted effluent. To elucidate the detoxification mechanisms underlying metal removal, various characterizations of the mycelium were conducted, including SEM, FTIR, and XRD analyses. Furthermore, LC–MS analysis shed light on the pivotal role of metabolites in regulating heavy metals within the physiological metabolism of P. opuntiae. Moreover, an upsurge in the concentration of the stress marker, metallothionein, and augmented activity of antioxidant enzymes, like SOD, CAT, LPO and GSH, collectively suggested the significant role of antioxidants in mitigating reactive oxygen species (ROS) and heavy metal toxicity. These comprehensive findings provide a solid foundation for understanding the mechanisms responsible for heavy metal removal by P. opuntiae and pave the way for the development of effective remediation strategies for decontaminating the effluents discharged by the leather industry, contributing to the preservation of our environment and to public well-being. [ABSTRACT FROM AUTHOR]

We developed a two-step dual-layer solid-phase extraction (SPE) method to separate and concentrate minor bioactive compounds of golden oyster mushroom (Pleurotus citrinopileatus) methanol extract. The mushroom extract is rich in fatty acids, including the highly abundant (20–35 %) and antibacterial linoleic acid. To eliminate the masking effect of linoleic acid in the bioassay, its removal was achieved by homemade dual-layer SPE that consisted of a lower C18 phase and an upper silica gel phase with the dried-on extract. In the first step the elution was performed with water (aqueous eluate), and then the dried C18 and silica gel phases were eluted separately with methanol (C18 methanol eluate and silica gel methanol eluate, respectively). Thin-layer chromatography (TLC)-effect-directed analysis demonstrated that the aqueous eluate contained sugars and antioxidants (TLC-DPPH); fatty acids were present in the silica gel methanol eluate, in the C18 methanol eluate the biologically active minor secondary metabolites were concentrated (TLC–Bacillus subtilis assay) beside 2 % remained linoleic acid according to HPLC-UV measurement. As a result, the antioxidant and antibacterial metabolites divided, and the low-abundant antibacterial compounds could be detected. Based on this study, the substance found at RF 0.25 by TLC-DPPH played the most significant role in generating the antioxidant effect of the aqueous eluate, equivalent to 5.07 ± 0.09 µg of gallic acid. Moreover, each SPE fraction and raw extract were tested for antibacterial effect using a microplate assay with a non-pathogenic Gram-positive Bacillus subtilis. The P. citrinopileatus extract (MIC-value – 50 µg/mL) and C18 methanol eluate (MIC-value - 25 µg/mL) inhibited the growth of B. subtilis while the other eluates did not.

Pleurotus ostreatus is a white-rot fungus that can degrade lignin in a preferential manner using a variety of extracellular enzymes, including manganese and versatile peroxidases (encoded by the vp1-3 and mnp1-6 genes, respectively). This fungus also secretes a family of structurally related small secreted proteins (SSPs) encoded by the ssp1-6 genes. Using RNA sequencing (RNA-seq), we determined that ssp4 and ssp6 are the predominant members of this gene family that were expressed by P. ostreatus during the first three weeks of growth on wheat straw. Downregulation of ssp4 in a strain harboring an ssp RNAi construct (KDssp1) was then confirmed, which, along with an increase in ssp6 transcript levels, coincided with reduced lignin degradation and the downregulation of vp2 and mnp1. In contrast, we observed an increase in the expression of genes related to pectin and side-chain hemicellulose degradation, which was accompanied by an increase in extracellular pectin-degrading capacity. Genome-wide comparisons between the KDssp1 and the wild-type strains demonstrated that ssp silencing conferred accumulated changes in gene expression at the advanced cultivation stages in an adaptive rather than an inductive mode of transcriptional response. Based on co-expression networking, crucial gene modules were identified and linked to the ssp knockdown genotype at different cultivation times. Based on these data, as well as previous studies, we propose that P. ostreatus SSPs have potential roles in modulating the lignocellulolytic and pectinolytic systems, as well as a variety of fundamental biological processes related to fungal growth and development.

Pleurotus ostreatus, due to its saprophytic nature, can extract nutrients and bioactive compounds from the substrate on which it is grown. This study aimed to assess the effect of adding spent coffee grounds (SCG) and potato peel (PPW) in the wheat straw substrate formulation to grow over the production indicators, physicochemical, techno-functional, total chemical compounds, and antioxidant properties. Treatments were described as follows: T1, wheat straw at 100%; T2, wheat straw at 80% + 10% of SCG + 10% of PPW; T3, wheat straw at 70% + 15% of SCG + 15% of PPW; T4, wheat straw at 60% + 20% of SCG + 20% of PPW. After P. ostreatus growth, non-differences were found in production indicators for T1–T4, including biological efficiency, production rate, and yield. With respect to P. ostreatus dried powders, T1–T4 showed pH values near neutrality concerning soy protein (SP), and the color samples were beige. Also, T2 and T3 exert higher water-holding (WHC) values, while T1–T4 exert higher oil-holding (OHC) and emulsifying capacity (EC) values concerning SP, in dependence on the growth substrate. T1–T4 showed lower swelling (SC) and T1–T3 lower gelling capacity (GC) values. Regarding total chemical compounds and antioxidant properties of P. ostreatus extracts, growth substrate and solvent extraction have an effect on metabolite content and antiradical and reducing power properties. The multivariate analysis revealed that T2 water extracts exert the highest total tannin (TTC) and protocatechuic acid contents (PAC), as well as the highest antiradical (RCSA) and reducing power (RPA) values. In conclusion, this study demonstrated that using SCG and PPW as a partial substitute for substrate (what straw) enhances the physicochemical, techno-functional, and antioxidant activity of P. ostreatus.

It is critical to prepare self-supported carbonaceous electrode materials that enable high-mass loading and efficient ion/electron transport through a simple and sustainable method. Herein, for the first time, heteroatom-doped (O, N, and S) hierarchically porous thick bulk carbon is synthesized by carbonizing Pleurotus eryngii (PE) and lignosulfonate (LS) mixed precursors. After carbonization, the generated thick bulk carbon consists of interconnected macropores at the micrometer scale, with micropores and mesopores densely covering the pore walls. The carbon material displays a large specific surface area ranging from 315.5 to 960.2 m2 g−1 and is uniformly doped with O (14.8–19.1%), N (1.73–2.97%) and S (1.20–1.94%), endowing it with excellent ion transport and charge storage properties. As a result, the obtained thick bulk carbon electrode with a mass loading of 58.0 mg cm−2 achieves an outstanding capacitance of 25.0 F cm−2 (112.0 F cm−3) at 10 mA cm−3 and a superior rate capability of 14.3 F cm−2 (66.9 F cm−3) at 200 mA cm−3. The assembled symmetric supercapacitor device delivers a high areal energy density of 2.56 mWh cm−2 at a power density of 0.64 mW cm−2 with an excellent stability of 91.1% after 15 000 cycles at 50 mA cm−2. This facile and green synthesis strategy provides new carbonaceous electrode design concepts for high-energy-density storage. [ABSTRACT FROM AUTHOR]

Pleurotus eryngii (PE) has been sought after for its various health benefits and high content of phenolic compounds. This study explored the feasibility of steam explosion (SE)-assisted extraction of polysaccharides with high antioxidant capacities from PE. An orthogonal experimental design (OED) was used to optimize the SE-assisted extraction of PE. The influence of the optimized SE-assisted extraction on the physicochemical properties of PE polysaccharides was determined by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), monosaccharide compositional analysis and antioxidant capacity assays. Under optimal SE conditions, SE-assisted extraction increased the polysaccharide yield by 138% compared to extraction without SE-assistance. In addition, SEM demonstrated that SE-assisted extraction markedly altered the spatial structure of Pleurotus eryngii polysaccharides (PEP), and monosaccharide compositional analysis revealed that this pretreatment significantly increased the proportions of some monosaccharides, such as glucose, rhamnose and arabinose, in the isolated PEP. FTIR spectra indicated no change in the major chemical functional groups of PEP. PEP extracted by SE-assisted extraction had significantly increased free radical scavenging and antioxidant capacities. In conclusion, SE-assisted extraction appears to be a novel polysaccharide extraction technology, which markedly increases extraction yields and efficiency and can increase the biological activity of polysaccharide extracts. [ABSTRACT FROM AUTHOR]

Pleurotus eryngii is an edible mushroom that suffers significant losses due to fungal contamination and bacteriosis. The Pseudomonadaceae family represents one of the most frequent etiologic agents. Grapefruit seed extract (GSE) is a plant extract that contains different bioactive components, such as naringin, and exhibits a strong antibacterial and antioxidant activity. Over the last decade, GSE use as an alternative to chemical treatments in the food sector has been tested. However, to our knowledge, its application on mushroom crops has never been investigated. This study focuses on evaluating GSE efficacy in preventing P. eryngii yellowing. GSE antibiotic activity, inhibitory and bactericidal concentrations, and antibiofilm activity against several microorganisms were tested with the Kirby–Bauer disk diffusion assay, the broth microdilution susceptibility test, and the Crystal violet assay, respectively. In vitro, the extract exhibited antimicrobial and antibiofilm activity against Staphylococcus aureus 6538 and MRSA (wild type), Escherichia coli ATCC 8739, and Pseudomonas spp. (Pseudomonas aeruginosa 9027, P. fluorescens (wild type)). GSE application in vivo, in pre- and post-sprouting stages, effectively prevented bacterial infections and subsequent degradation in the mushroom crops: none of the P. eryngii treated manifested bacteriosis. Our findings support the use of GSE as an eco-friendly and sustainable alternative to chemical treatments for protecting P. eryngii crops from bacterial contamination, consequently ensuring food safety and preventing financial losses due to spoilage. Furthermore, GSE's potential health benefits due to its content in naringin and other bioactive components present new possibilities for its use as a nutraceutical in food fortification and supplementation. [ABSTRACT FROM AUTHOR]

Pleurotus citrinopileatus is a low-cholesterol, protein-rich, and high-nutrient food. The molecular mechanisms of the compounds and coloration have not been reported. Metabolome and transcriptome were used to clarify the molecular mechanisms of key compounds biosynthesis. K-means analysis identified 19 compounds in P. citrinopileatus , mainly lipids and alkaloids in class 8. In addition, 84 lipids were higher and that the different compounds were mainly enriched in linoleic acid metabolism. A total of 14 compounds detected in the linoleic acid metabolism pathway were significantly up-regulated, while 3 sterol regulatory element binding protein (SREBP) transcription factors were screened. Tryptophan metabolism and riboflavin biosynthesis pathway analysis indicated that 3 Unigenes had tryptophan decarboxylase similar elements, which belonged to tyrosine decarboxylase 1. Moreover, CL15618.Contig5_All had high homology with MFS. In conclusion, the expression of 3 SREBP, the synthesis of isobavachalcone D, and the regulation of riboflavin transport by MCH5 were the reasons for fatty acid accumulation and yellow cap formation in the P. citrinopileatus. [ABSTRACT FROM AUTHOR]