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The hybrid electric train which operates without overhead wires or traditional power sources relies on hydrogen fuel cells and batteries for power. These fuel cell-based hybrid electric trains (FCHETs) are more efficient than those powered by diesel or electricity because they do not produce any tailpipe emissions making them an eco-friendly mode of transport. The target of this paper is to propose low-budget FCHETs that prioritize energy efficiency to reduce operating costs and minimize their impact on the environment. To this end, an energy management strategy [EMS] has been developed that optimizes the distribution of energy to reduce the amount of hydrogen required to power the train. The EMS achieves this by balancing battery charging and discharging. To enhance the performance of the EMS, proposes to use of a deep reinforcement learning (DRL) algorithm specifically the deep deterministic policy gradient (DDPG) combined with transfer learning (TL) which can improve the system's efficiency when driving cycles are changed. DRL-based strategies are commonly used in energy management and they suffer from unstable convergence, slow learning speed, and insufficient constraint capability. To address these limitations, an action masking technique to stop the DDPG-based approach from producing incorrect actions that go against the system's physical limits and prevent them from being generated is proposed. The DDPG+TL agent consumes up to 3.9% less energy than conventional rulebased EMS while maintaining the battery's charge level within a predetermined range. The results show that DDPG+TL can sustain battery charge at minimal hydrogen consumption with minimal training time for the agent. [ABSTRACT FROM AUTHOR]

Molecular markers have been found useful in assessing the genetic diversity in a precise manner, which is based on naturally occurring DNA polymorphism. Random Amplified Polymorphic DNA analysis was performed using four arbitary decamer primers to observe genetic homogeneity/ polymorphism among 10 isolates of fluorescent Pseudomonas spp. isolated from apple and pear rhizosphere from different locations of Mandi and Chamba districts. Out of 52 bands generated with 4 RAPD primers, 13.46% were monomorphic bands, while 86.33% were polymorphic bands. The result showed that the isolates of fluorescent Pseudomonas sp. were divided into 2 clusters by RAPD primers. Among all the combinations maximum similarity (90.48%) was found between PN-7-Cha and PN-2-San isolates of Chamba and Mandi districts. However, minimum similarity (36.7%) was observed in PN-11-San and PN- 7-Cha isolates of Mandi and Chamba districts. This technique is highly beneficial for testing similarity analysis among fluorescent Pseudomonas spp. isolated from different locations. [ABSTRACT FROM AUTHOR]