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Abstract Background Metal oxide nanoparticles (NPs) are becoming valuable due to their novel applications. The green synthesis of TiO2 NPs is more popular as a flexible and eco-friendly method compared to traditional chemical synthesis methods. TiO2 NPs are the most commonly used semiconductor in dye-sensitized solar cells (DSSCs). Results The biogenic TiO2 NPs were produced extracellularly by the marine halophilic bacterium Halomonas sp. RAM2. Response surface methodology (RSM) was used to optimize the biosynthesis process, resulting in a starting TiO2 concentration of 0.031 M and a pH of 5 for 92 min (⁓15 nm). TiO2 NPs were well-characterized after the calcination process at different temperatures of 500, 600, 700 and 800 °C. Anatase TiO2 NPs (calcined at 500 °C) with a smaller surface area and a wider bandgap were nominated for use in natural dye-sensitized solar cells (NDSSCs). The natural dye used as a photosensitizer is a mixture of three carotenoids extracted from the marine bacterium Kocuria sp. RAM1. NDSSCs were evaluated under standard illumination. After optimization of the counter electrode, NDSSCBio(10) (10 layers) demonstrated the highest photoelectric conversion efficiency (η) of 0.44%, which was almost as good as NDSSCP25 (0.55%). Conclusion The obtained results confirmed the successful green synthesis of TiO2 NPs and suggested a novel use in combination with bacterial carotenoids in DSSC fabrication, which represents an initial step for further efficiency enhancement studies.

Abstract Marine pigmented bacteria are a promising natural source of carotenoids. Kocuria sp. RAM1 was isolated from the Red Sea Bohadschia graeffei collected from Marsa Alam, Egypt, and used for carotenoids production. The extracted carotenoids were purified by thin-layer chromatography (TLC). The characteristic UV absorbance of the three purified fractions gave us an inkling of what the purified pigments were. The chemical structures were confirmed by nuclear magnetic resonance spectroscopy (NMR) and LC-ESI-QTOF-MS/MS. The three different red pigments were identified as two C50-carotenoids, namely bisanhydrobacterioruberin and trisanhydrobacterioruberin, in addition to 3,4,3ʹ,4ʹ-Tetrahydrospirilloxanthin (C42-carotenoids). Kocuria sp. RAM1 carotenoids were investigated for multiple activities, including antimicrobial, anti-inflammatory, antioxidant, anti-HSV-1, anticancer, antidiabetic and wound healing. These new observations suggest that Kocuria sp. RAM1 carotenoids can be used as a distinctive natural pigment with potent properties.

The enthusiasm for natural dyes applications increased thanks to biodegradable and eco-friendly properties. In this study, prodigiosin was extracted from Serratia rubidaea RAM_Alex. This red pigment was used in dyeing different textile fabrics. Synthetic fabrics had a higher affinity to the natural dye compared to cotton fabrics. Copper sulfate proved to be an effective mordant. Fabrics dyed with prodigiosin showed a considerable degree of fastness to light and washing. Dyed samples with prodigiosin have antimicrobial activity, which is varied in consonance with the nature of the fabric and microbial species. Both Escherichia coli and Staphylococcus aureus were inhibited. Application of the natural pigment prodigiosin to certain textiles particularly those used in hospitals would lead to a significant decrease in hospital-acquired infections (HAI), patient outcomes improvement and economical saving.

This research sought to determine optimal conditions to maximize prodigiosin production by an indigenous Egyptian marine bacterial strain Serratia rubidaea RAM_Alex. Serratia rubidaea RAM_Alex isolated from bivalve samples of Temsah Lake, Ismailia, Egypt was used to investigate the production of the natural red pigment prodigiosin. Pigment production was assayed in different growth conditions using Nutrient broth as production medium. The water insoluble red pigment was extracted using ethanol and further purified by organic solvents. The pigment extract showed absorbance with a UV-Vis spectrophotometer at 535 nm and further characterized using TLC, FTIR and 1H-NMR. A statistical screening procedure was adopted to select the main factors affecting production. Analyses of Plackett- Burman design results demonstrated that peptone, NaCl, and culture volume were the most important independent variables. The near optimum medium contained (g/L): peptone 7, beef extract 5, yeast extract 1, NaCl 10, pH 6, using 25 ml culture volume, 100 ¼l inoculum size and incubation statically for 48 h at 30oC. When this condition was employed, a two fold increase in pigment yield was achieved reaching ~1600.511 mg/l.