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The growing global demand for food has led the search for new sources of highly nutritious biomass. Cyanobacteria can be used not only as a source of compounds of biotechnological interest, but also as food for animals and humans, because of their high nutritional quality. The aim of this work was to evaluate the nutritional quality of biomass from four strains of nitrogenfixing filamentous cyanobacteria in batch cultures. Nostoc LAUN015, Nostoc UAM206, Anabaena MOF015 and Anabaena MOF016 were cultured in diazotrophic conditions at laboratory scale. The biomass was harvested, dried and used for biochemical evaluation, proximal, microbiological and toxicological quality. Biomass protein has values between 25 and 40%, 43-48% of carbohydrates, 0.39 to 1.64% of fiber and 0.74-1.05% of lipids. Biomass is rich in amino acids, specially arginine, lysine, aspartate and glutamate. Pathogens such as S. aureus, total and fecal coliforms, E. coli and Salmonella, were undetected. Toxicology tests showed high LC50 for Anabaena MOF015, indicating no toxicity. In conclusion, Anabaena MOF015 stands within the group, for having an excellent biomass production, nutritional quality and no toxicity detected, and could be used as a protein source for human and animal nutrition. [ABSTRACT FROM AUTHOR]