14 results on '"Saporito, M."'
Search Results
2. Surgical time for graft preparation using different suture techniques
- Author
-
Camarda, L., primary, Giambartino, S., additional, Lauria, M., additional, Saporito, M., additional, Triolo, V., additional, and D’Arienzo, M., additional
- Published
- 2019
- Full Text
- View/download PDF
3. Predicting the failure in distal femur fractures
- Author
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Peschiera, V., primary, Staletti, L., additional, Cavanna, M., additional, Saporito, M., additional, and Berlusconi, M., additional
- Published
- 2018
- Full Text
- View/download PDF
4. Snapping Triceps
- Author
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Ciaramella, G., Saporito, M., Fauci, F., and Porcellini, G.
- Published
- 2017
5. An Update of the Evolving Epidemic of blaKPC Carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of Multiple Non-ST258 Clones
- Author
-
BONURA, Celestino, GIUFFRE, Mario, ALEO, Aurora, Fasciana, Teresa Maria Assunta, Di Bernardo, F., Stampone, T., GIAMMANCO, Anna, Palma, D., MAMMINA, Caterina, GERACI, Daniela Maria, GRAZIANO, Giorgio, SAPORITO, Laura, Saporito, M., Sodano, C., Verde, M., Baldi, M., Donato, E., Amato, T., Immordino, R., Distefano, S., Bonura, C., Giuffrè, M., Aleo, A., Fasciana, T., Di Bernardo, F., Stampone, T., Giammanco, A., Palma, D., Mammina, C., Geraci, D., Graziano, G., Saporito, L., Saporito, M., Sodano, C., Verde, M., Baldi, M., Donato, E., Amato, T., Immordino, R., and Distefano, S.
- Subjects
Carbapenem ,Klebsiella pneumoniae ,lcsh:Medicine ,Gene Expression ,Drug resistance ,Plasmid ,beta-Lactamase ,Disease Outbreaks ,Molecular typing ,Fluoroquinolone ,Drug Resistance, Multiple, Bacterial ,polycyclic compounds ,lcsh:Science ,Membrane Protein ,Disease Outbreak ,Multidisciplinary ,biology ,Medicine (all) ,Incidence ,Hospitals ,Anti-Bacterial Agents ,Electrophoresis, Gel, Pulsed-Field ,Italy ,Epidemiological Monitoring ,Human ,Fluoroquinolones ,Plasmids ,Research Article ,medicine.drug ,Bacterial Protein ,Aminoglycosides ,Bacterial Proteins ,Carbapenems ,Clone Cells ,Colistin ,Humans ,Klebsiella Infections ,Membrane Proteins ,Multilocus Sequence Typing ,Mutation ,beta-Lactamases ,Agricultural and Biological Sciences (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Microbiology ,Clone Cell ,Hospital ,Antibiotic resistance ,Anti-Bacterial Agent ,medicine ,Aminoglycoside ,lcsh:R ,Carbapenemase producing ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,Virology ,Multilocus sequence typing ,lcsh:Q ,Klebsiella Infection - Abstract
Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute general hospitals in Palermo, Italy, in the six-month period March-August 2014. They were characterized by PCR for β-lactam, aminoglycoside and plasmid mediated fluoroquinolone resistance genetic determinants. The mgrB gene of the colistin resistant isolates was amplified and sequenced. Clonality was assessed by pulsed field gel electrophoresis and multilocus sequence typing. Eight non-CC258 sequence types (STs) were identified accounting for 60% of isolates. In particular, ST307 and ST273 accounted for 29% and 18% of isolates. CC258 isolates were more frequently susceptible to gentamicin and non-CC258 isolates to amikacin. Colistin non susceptibility was found in 42% of isolates. Modifications of mgrB were found in 32 isolates. Conclusions: Concurrent clonal expansion of some STs and lateral transmission of genetic resistance determinants are likely producing a thorough change of the KPC-Kp epidemiology in Palermo, Italy. In our setting mgrB inactivation proved to substantially contribute to colistin resistance. Our findings suggest the need to continuously monitor the KPC-Kp epidemiology and to assess by a nationwide survey the possible shifting towards a polyclonal epidemic.
- Published
- 2015
6. DVWA gene polymorphisms and osteoarthritis
- Author
-
Bravatà, V, Minafra, L, Forte, G, Cammarata, F, Saporito, M, Boniforti, F, Lio, D, Gilardi, M, Messa, M, GILARDI, MARIA CARLA, MESSA, MARIA CRISTINA, Bravatà, V, Minafra, L, Forte, G, Cammarata, F, Saporito, M, Boniforti, F, Lio, D, Gilardi, M, Messa, M, GILARDI, MARIA CARLA, and MESSA, MARIA CRISTINA
- Abstract
Background: Osteoarthritis (OA) is a degenerative joints disorder influenced by genetic predisposition. We reported that rs11718863 DVWA SNP was represented in Sicilian with a more severe Kellgren and Lawrence (KL) radiographic grade, displaying its predictive role as OA marker progression. Here, we describe the DVWA SNPs: rs11718863, rs7639618, rs7651842, rs7639807 and rs17040821 probably able to induce protein functional changes. Findings: Sixty-one Sicilian patients with knee OA and 100 healthy subjects were enrolled. Clinical and radiographic evaluation was performed using AKSS scores and KL. Linkage Disequilibrium (LD) analyses were performed in order to verify whether the SNPs segregate as haplotype. All DVWA SNPs'MinorAllele Frequencies (MAF) were greater than in the European. The rs7639618 SNP showed a statistical association with KL. Our analyses show that a LD exists among rs11718863 and rs7639618, as well as between rs7651842, rs7639807 and rs17040821 SNPs. We also observed that three out of the 161 individuals investigated were simultaneously homozygous carriers of the rs7651842, rs7639807 and rs17040821 MAF alleles. Conclusions: In summary, the purpose of this preliminary research was to highlight possible associations between DVWA SNPs and OA clinical and radiographic data. This work represents a multidisciplinary medicine approach to study OA where clinical, radiological and genetic evaluation could contribute to better define OA grading.
- Published
- 2015
7. Auditoria como Ferramenta no Controle da Qualidade
- Author
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Saporito, M. F. M. W., primary and Villela, R. D., additional
- Published
- 2015
- Full Text
- View/download PDF
8. Surgical time for graft preparation using different suture techniques
- Author
-
Michele Saporito, Sebastian Giambartino, Lawrence Camarda, Michele Lauria, Vito Triolo, Michele D'Arienzo, Camarda, L., Giambartino, S., Lauria, M., Saporito, M., Triolo, V., and D'Arienzo, M
- Subjects
Orthodontics ,Suturing techniques ,030222 orthopedics ,medicine.medical_specialty ,business.industry ,modified finger-trap, suture, time consuming, krakow stitch, whipstitch ,Interobserver reproducibility ,Surgery ,Tendon ,03 medical and health sciences ,Surgical time ,0302 clinical medicine ,medicine.anatomical_structure ,Suture (anatomy) ,Settore MED/33 - Malattie Apparato Locomotore ,Medicine ,Operative time ,Original Article ,Orthopedics and Sports Medicine ,030212 general & internal medicine ,business - Abstract
Background: The purpose of the present study was to compare the operative time for graft prepa- ration using different techniques for graft sutur- ing. Material and methods: Flexor profundus tendons were harvested from fresh pig hind-leg trotters. Three different suture techniques were investigat- ed: the Krackow stitch (K), the Whipstitch (W), and the Modified Finger-Trap suture (MFT). Ten- dons were sutured starting at 10 mm from the dis- tal free end of the tendon. The suture configura- tions of the Krackow stitch and Whipstitch were completed with five suture throws. According to the MFT technique, the suture was wrapped five times around the tendon over a distance of 30 mm. The time required to perform a complete su- ture on each tendon was measured. Five indepen- dent examiners of different levels of training mea- sured the time required for graft preparation dur- ing 3 separate occasions to determine intraob- server repeatability and interobserver reproduci- bility. Results: The mean time required for graft prepa- ration following the Krackow technique was 69.1 seconds ± 18.3 SD (range 31.8-120). The Whip- stitch technique took an average of 59.9 seconds ± 21.2 SD (range 27-93). The MFT suture required a mean of 29.3 seconds ± 11.4 SD for completing the suture (range 21.6-33). In all examiners the time required to complete the MFT suture was significantly less than the other suture tech- niques (p < 0.05). Intraobserver intraclass correla- tion coefficients for each examiner ranged from 0.72 to 0.83. Conclusion: Low graft preparation time is re- quired to complete a MFT suture in a porcine ten- don model. Further, time required for graft prepa- ration using the MFT was shorter than other su- turing techniques such as the Krackow and Whip- stitch techniques. Clinical relevance: The MFT suture could be used for graft set-up with the main advantage of reduc- ing the time required in comparison with other su- ture techniques.
- Published
- 2019
9. DVWA gene polymorphisms and osteoarthritis
- Author
-
Valentina Bravatà 1, Luigi Minafra 1, Giusi I Forte 1, Francesco P Cammarata 1, Michele Saporito 2, Filippo Boniforti 3, Domenico Lio 4, Maria C Gilardi 1, 5, 6, Cristina Messa 1, 7, BRAVATA', V, Minafra, L, Forte, GI, Cammarata, FP, Saporito, M, Boniforti, F, Lio, D, Gilardi, MC, Messa, C, Bravatà, V, Forte, G, Cammarata, F, Gilardi, M, and Messa, M
- Subjects
Male ,Linkage disequilibrium ,Short Report ,Single-nucleotide polymorphism ,Osteoarthritis ,Collagen Type VI ,Bioinformatics ,Polymorphism, Single Nucleotide ,General Biochemistry, Genetics and Molecular Biology ,Linkage Disequilibrium ,White People ,Gene Frequency ,Haplotype ,Genetic predisposition ,DVWA ,Medicine ,SNP ,Humans ,Genetic Predisposition to Disease ,Allele ,Osteoarthritis, DVWA, Single nucleotide polymorphisms, Haplotypes, KL ,Allele frequency ,Sicily ,Alleles ,Aged ,Medicine(all) ,Aged, 80 and over ,Biochemistry, Genetics and Molecular Biology(all) ,business.industry ,Homozygote ,General Medicine ,Single nucleotide polymorphisms ,Middle Aged ,Osteoarthritis, Knee ,medicine.disease ,Single nucleotide polymorphism ,KL ,Haplotypes ,Osteoarthriti ,Female ,business ,Pseudogenes - Abstract
Background: Osteoarthritis (OA) is a degenerative joints disorder influenced by genetic predisposition. We reported that rs11718863 DVWA SNP was represented in Sicilian with a more severe Kellgren and Lawrence (KL) radiographic grade, displaying its predictive role as OA marker progression. Here, we describe the DVWA SNPs: rs11718863, rs7639618, rs7651842, rs7639807 and rs17040821 probably able to induce protein functional changes. Findings: Sixty-one Sicilian patients with knee OA and 100 healthy subjects were enrolled. Clinical and radiographic evaluation was performed using AKSS scores and KL. Linkage Disequilibrium (LD) analyses were performed in order to verify whether the SNPs segregate as haplotype. All DVWA SNPs'MinorAllele Frequencies (MAF) were greater than in the European. The rs7639618 SNP showed a statistical association with KL. Our analyses show that a LD exists among rs11718863 and rs7639618, as well as between rs7651842, rs7639807 and rs17040821 SNPs. We also observed that three out of the 161 individuals investigated were simultaneously homozygous carriers of the rs7651842, rs7639807 and rs17040821 MAF alleles. Conclusions: In summary, the purpose of this preliminary research was to highlight possible associations between DVWA SNPs and OA clinical and radiographic data. This work represents a multidisciplinary medicine approach to study OA where clinical, radiological and genetic evaluation could contribute to better define OA grading.
- Published
- 2015
10. Complex Metabolism of the Novel Neurosteroid, Ganaxolone, in Humans: A Unique Challenge for Metabolites in Safety Testing Assessment.
- Author
-
Fitch WL, Smith S, Saporito M, Busse G, Zhang M, Ren J, Fitzsimmons ME, Yi P, English S, Carter A, and Baillie TA
- Subjects
- Animals, Humans, Male, Pregnanolone analysis, Mass Spectrometry, Chromatography, Liquid, Chromatography, High Pressure Liquid, Feces chemistry, Neurosteroids analysis
- Abstract
The human pharmacokinetics, metabolism, and excretion of [
14 C]-ganaxolone (GNX) were characterized in healthy male subjects ( n = 8) following a single 300-mg (150 μ Ci) oral dose. GNX exhibited a short half-life of 4 hours in plasma, whereas total radioactivity had a half-life of 413 hours indicating extensive metabolism to long-lived metabolites. Identification of the major GNX circulating metabolites required extensive isolation and purification for liquid chromatography-tandem mass spectrometry analysis, together with in vitro studies, NMR spectroscopy, and synthetic chemistry support. This revealed that the major routes of GNX metabolism involved hydroxylation at the 16 α -hydroxy position, stereoselective reduction of the 20-ketone to afford the corresponding 20 α -hydroxysterol, and sulfation of the 3 α -hydroxy group. This latter reaction yielded an unstable tertiary sulfate, which eliminated the elements of H2 SO4 to introduce a double bond in the A ring. A combination of these pathways, together with oxidation of the 3 β -methyl substituent to a carboxylic acid and sulfation at the 20 α position, led to the major circulating metabolites in plasma, termed M2 and M17. These studies, which led to the complete or partial identification of no less than 59 metabolites of GNX, demonstrated the high complexity of the metabolic fate of this drug in humans and demonstrated that the major circulating products in plasma can result from multiple sequential processes that may not be easily replicated in animals or with animal or human in vitro systems. SIGNIFICANCE STATEMENT: Studies on the metabolism of [14 C]-ganaxolone in humans revealed a complex array of products that circulated in plasma, the two major components of which were formed via an unexpected multi-step pathway. Complete structural characterization of these (disproportionate) human metabolites required extensive in vitro studies, along with contemporary mass spectrometry, NMR spectroscopy, and synthetic chemistry efforts, which served to underscore the limitations of traditional animal studies in predicting major circulating metabolites in man., (Copyright © 2023 by The American Society for Pharmacology and Experimental Therapeutics.)- Published
- 2023
- Full Text
- View/download PDF
11. Functional characterization of a novel p.Ser76Thr variant in IGFBP4 that associates with body mass index in American Indians.
- Author
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Muller YL, Saporito M, Day S, Bandesh K, Koroglu C, Kobes S, Knowler WC, Hanson RL, Van Hout CV, Shuldiner AR, Bogardus C, and Baier LJ
- Subjects
- Animals, Body Mass Index, Humans, Mice, Obesity genetics, PPAR gamma genetics, Phosphatidylinositol 3-Kinases genetics, Polymorphism, Single Nucleotide, Proto-Oncogene Proteins c-akt genetics, American Indian or Alaska Native, Indians, North American genetics, Insulin-Like Growth Factor Binding Protein 4 genetics
- Abstract
Insulin-like growth factor binding protein 4 (IGFBP4) is involved in adipogenesis, and IGFBP4 null mice have decreased body fat through decreased PPAR-γ expression. In the current study, we assessed whether variation in the IGFBP4 coding region influences body mass index (BMI) in American Indians who are disproportionately affected by obesity. Whole exome sequence data from a population-based sample of 6779 American Indians with longitudinal measures of BMI were used to identify variation in IGFBP4 that associated with BMI. A novel variant that predicts a p.Ser76Thr in IGFBP4 (Thr-allele frequency = 0.02) was identified which associated with the maximum BMI measured during adulthood (BMI 39.8 kg/m
2 for Thr-allele homozygotes combined with heterozygotes vs. 36.2 kg/m2 for Ser-allele homozygotes, β = 6.7% per Thr-allele, p = 8.0 × 10-5 , adjusted for age, sex, birth-year and the first five genetic principal components) and the maximum age- and sex-adjusted BMI z-score measured during childhood/adolescence (z-score 0.70 SD for Thr-allele heterozygotes vs. 0.32 SD for Ser-allele homozygotes, β = 0.37 SD per Thr-allele, p = 8.8 × 10-6 ). In vitro functional studies showed that IGFBP4 with the Thr-allele (BMI-increasing) had a 55% decrease (p = 0.0007) in FOXO-induced transcriptional activity, reflecting increased activation of the PI3K/AKT pathway mediated through increased IGF signaling. Over-expression and knock-down of IGFBP4 in OP9 cells during differentiation showed that IGFBP4 upregulates adipogenesis through PPARγ, CEBPα, AGPAT2 and SREBP1 expression. We propose that this American Indian specific variant in IGFBP4 affects obesity via an increase of IGF signaling., (© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)- Published
- 2022
- Full Text
- View/download PDF
12. Surgical time for graft preparation using different suture techniques.
- Author
-
Camarda L, Giambartino S, Lauria M, Saporito M, Triolo V, and D'Arienzo M
- Abstract
Background: The purpose of the present study was to compare the operative time for graft preparation using different techniques for graft suturing., Material and Methods: Flexor profundus tendons were harvested from fresh pig hind-leg trotters. Three different suture techniques were investigated: the Krackow stitch (K), the Whipstitch (W), and the Modified Finger-Trap suture (MFT). Tendons were sutured starting at 10 mm from the distal free end of the tendon. The suture configurations of the Krackow stitch and Whipstitch were completed with five suture throws. According to the MFT technique, the suture was wrapped five times around the tendon over a distance of 30 mm. The time required to perform a complete suture on each tendon was measured. Five independent examiners of different levels of training measured the time required for graft preparation during 3 separate occasions to determine intraobserver repeatability and interobserver reproducibility., Results: The mean time required for graft preparation following the Krackow technique was 69.1 seconds ± 18.3 SD (range 31.8-120). The Whipstitch technique took an average of 59.9 seconds ± 21.2 SD (range 27-93). The MFT suture required a mean of 29.3 seconds ± 11.4 SD for completing the suture (range 21.6-33). In all examiners the time required to complete the MFT suture was significantly less than the other suture techniques (p < 0.05). Intraobserver intraclass correlation coefficients for each examiner ranged from 0.72 to 0.83., Conclusion: Low graft preparation time is required to complete a MFT suture in a porcine tendon model. Further, time required for graft preparation using the MFT was shorter than other suturing techniques such as the Krackow and Whipstitch techniques., Clinical Relevance: The MFT suture could be used for graft set-up with the main advantage of reducing the time required in comparison with other suture techniques.
- Published
- 2016
- Full Text
- View/download PDF
13. Erythema Nodosum and Mycoplasma pneumoniae Infections in Childhood: Further Observations in Two Patients and a Literature Review.
- Author
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Greco F, Catania R, Pira AL, Saporito M, Scalora L, Aguglia MG, Smilari P, and Sorge G
- Abstract
Erythema nodosum (EN) is the most frequent panniculitis in childhood and has been associated with various conditions, such as infectious and autoimmune disorders, medications, and malignancies. The author reports on two children affected with EN associated with Mycoplasma pneumoniae infection, which occurred in one patient without pulmonary detection. The available literature on EN and M. pneumoniae infection in childhood is also reviewed.
- Published
- 2015
- Full Text
- View/download PDF
14. DVWA gene polymorphisms and osteoarthritis.
- Author
-
Bravatà V, Minafra L, Forte GI, Cammarata FP, Saporito M, Boniforti F, Lio D, Gilardi MC, and Messa C
- Subjects
- Aged, Aged, 80 and over, Alleles, Female, Gene Frequency, Haplotypes, Homozygote, Humans, Linkage Disequilibrium, Male, Middle Aged, Osteoarthritis, Knee ethnology, Osteoarthritis, Knee pathology, Sicily, White People, Collagen Type VI genetics, Genetic Predisposition to Disease, Osteoarthritis, Knee genetics, Polymorphism, Single Nucleotide, Pseudogenes genetics
- Abstract
Background: Osteoarthritis (OA) is a degenerative joints disorder influenced by genetic predisposition. We reported that rs11718863 DVWA SNP was represented in Sicilian with a more severe Kellgren and Lawrence (KL) radiographic grade, displaying its predictive role as OA marker progression. Here, we describe the DVWA SNPs: rs11718863, rs7639618, rs7651842, rs7639807 and rs17040821 probably able to induce protein functional changes., Findings: Sixty-one Sicilian patients with knee OA and 100 healthy subjects were enrolled. Clinical and radiographic evaluation was performed using AKSS scores and KL. Linkage Disequilibrium (LD) analyses were performed in order to verify whether the SNPs segregate as haplotype. All DVWA SNPs'MinorAllele Frequencies (MAF) were greater than in the European. The rs7639618 SNP showed a statistical association with KL. Our analyses show that a LD exists among rs11718863 and rs7639618, as well as between rs7651842, rs7639807 and rs17040821 SNPs. We also observed that three out of the 161 individuals investigated were simultaneously homozygous carriers of the rs7651842, rs7639807 and rs17040821 MAF alleles., Conclusions: In summary, the purpose of this preliminary research was to highlight possible associations between DVWA SNPs and OA clinical and radiographic data. This work represents a multidisciplinary medicine approach to study OA where clinical, radiological and genetic evaluation could contribute to better define OA grading.
- Published
- 2015
- Full Text
- View/download PDF
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