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Your search keyword '"Sethi, Dalip"' showing total 13 results
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13 results on '"Sethi, Dalip"'

10. Safety and Effectiveness of Bone Marrow Cell Concentrate in the Treatment of Chronic Critical Limb Ischemia Utilizing a Rapid Point-of-Care System

Author

Ponemone, Venkatesh, primary, Gupta, Saniya, additional, Sethi, Dalip, additional, Suthar, Manish, additional, Sharma, Monika, additional, Powell, Richard J., additional, Harris, Kenneth Lee, additional, Jungla, Nungshi, additional, Arambam, Priyadarshini, additional, Kaul, Upendra, additional, Seth, Ashok, additional, and Bukhari, Suhail, additional

Published
2017
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11. Effects of Hypoxanthine Substitution in Peptide Nucleic Acids Targeting KRAS2Oncogenic mRNA Molecules: Theory and Experiment

Author

Sanders, Jeffrey M., Wampole, Matthew E., Chen, Chang-Po, Sethi, Dalip, Singh, Amrita, Dupradeau, François-Yves, Wang, Fan, Gray, Brian D., Thakur, Mathew L., and Wickstrom, Eric

Abstract

Genetic disorders can arise from single base substitutions in a single gene. A single base substitution for wild type guanine in the twelfth codon of KRAS2mRNA occurs frequently to initiate lung, pancreatic, and colon cancer. We have observed single base mismatch specificity in radioimaging of mutant KRAS2mRNA in tumors in mice by in vivohybridization with radiolabeled peptide nucleic acid (PNA) dodecamers. We hypothesized that multimutant specificity could be achieved with a PNA dodecamer incorporating hypoxanthine, which can form Watson–Crick base pairs with adenine, cytosine, thymine, and uracil. Using molecular dynamics simulations and free energy calculations, we show that hypoxanthine substitutions in PNAs are tolerated in KRAS2RNA:PNA duplexes where wild type guanine is replaced by mutant uracil or adenine in RNA. To validate our predictions, we synthesized PNA dodecamers with hypoxanthine, and then measured the thermal stability of RNA:PNA duplexes. Circular dichroism thermal melting results showed that hypoxanthine-containing PNAs are more stable in duplexes where hypoxanthine-adenine and hypoxanthine-uracil base pairs are formed than single mismatch duplexes or duplexes containing hypoxanthine-guanine opposition.

Published
2024
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13. The monoculture of cord-blood-derived CD34 + cells by an automated, membrane-based dynamic perfusion system with a novel cytokine cocktail.

Author

Jones M, Cunningham A, Frank N, and Sethi D

Subjects
Humans, Hematopoietic Stem Cells metabolism, Cell Culture Techniques methods, Antigens, CD34 metabolism, Stem Cell Factor pharmacology, Stem Cell Factor metabolism, Perfusion, Cells, Cultured, Fetal Blood, Cytokines metabolism
Abstract

Human leukocyte antigen (HLA)-matched cord blood (CB) transplantation is a procedure for the treatment of certain hematological malignancies, hemoglobinopathies, and autoimmune disorders. However, one of the challenges is to provide a sufficient number of T cell-depleted hematopoietic stem and progenitor cells. Currently, only 4%-5% of the CB units stored in CB banks contain enough CD34 + cells for engrafting 70-kg patients. To support this clinical need, we have developed an automated expansion protocol for CB-derived CD34 + cells in the Quantum system's dynamic perfusion bioreactor using a novel cytokine cocktail comprised of stem cell factor (SCF), thrombopoietin (TPO), fms-like tyrosine kinase 3 ligand (Flt-3L), interleukin-3 (IL-3), IL-6, glial cell line-derived neurotrophic factor (GDNF), StemRegenin 1 (SR-1), and a fibronectin-stromal-cell-derived factor-1 (SDF-1)-coated membrane. In an 8-day expansion of a 2 × 10 6 positively selected CD34 + cell inoculum from 3 donor lineages, the mean cell harvest and cell viability were 1.02 × 10 8 cells and 95.5%, respectively, and the mean frequency of the CD45 + CD34 + immunophenotype was 54.3%. The mean differentiated cell frequencies were 0.5% for lymphocytes, 15.8% for neutrophils, and 15.4% for platelets. These results demonstrate that the automated monoculture protocol can support the expansion of CD34 + cells with minimal lymphocyte residual., Competing Interests: Conflict of interests M.J., A.C., N.F., and D.S. are all employees of Terumo Blood and Cell Technologies, Inc. (Lakewood, CO, USA) at the time of manuscript preparation., (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)

Published
2022
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