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Your search keyword '"Shao, Ningsheng"' showing total 36 results
Start Over Author "Shao, Ningsheng" Publication Year Range Last 10 years
36 results on '"Shao, Ningsheng"'

8. AGO4 suppresses tumor growth by modulating autophagy and apoptosis via enhancing TRIM21-mediated ubiquitination of GRP78 in a p53-independent manner

Author

Wang, Lin, primary, Li, Da, additional, Su, Xueting, additional, Zhao, Yuechao, additional, Huang, Aixue, additional, Li, Hui, additional, Li, Jie, additional, Xia, Wei, additional, Jia, Tianqi, additional, Zhang, Hongwen, additional, Dong, Jie, additional, Liu, Xuemei, additional, and Shao, Ningsheng, additional

Published
2022
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10. Aptamer-functionalized lipid nanoparticles targeting osteoblasts as a novel RNA interference-based bone anabolic strategy

Author

Liang, Chao, Guo, Baosheng, Wu, Heng, Shao, Ningsheng, Li, Defang, Liu, Jin, Dang, Lei, Wang, Cheng, Li, Hui, Li, Shaohua, Lau, Wing Ki, Cao, Yu, Yang, Zhijun, Lu, Cheng, He, Xiaojuan, Au, D.W.T., Pan, Xiaohua, Zhang, Bao-Ting, Lu, Changwei, Zhang, Hongqi, Yue, Kinman, Qian, Airong, Shang, Peng, Xu, Jiake, Xiao, Lianbo, Bian, Zhaoxiang, Tan, Weihong, Liang, Zicai, He, Fuchu, Zhang, Lingqiang, Lu, Aiping, and Zhang, Ge

Subjects
Nanoparticles -- Usage -- Health aspects, Osteoblasts -- Physiological aspects -- Genetic aspects -- Research, Biological sciences, Health
Abstract

Currently, major concerns about the safety and efficacy of RNA interference (RNAi)-based bone anabolic strategies still exist because of the lack of direct osteoblast-specific delivery systems for osteogenic siRNAs. Here we screened the aptamer CH6 by cell-SELEX, specifically targeting both rat and human osteoblasts, and then we developed CH6 aptamer-functionalized lipid nanoparticles (LNPs) encapsulating osteogenic pleckstrin homology domain-containing family O member 1 (Plekho1) siRNA (CH6-LNPs-siRNA). Our results showed that CH6 facilitated in vitro osteoblast-selective uptake of Plekho1 siRNA, mainly via macropinocytosis, and boosted in vivo osteoblast-specific Plekhol gene silencing, which promoted bone formation, improved bone microarchitecture, increased bone mass and enhanced mechanical properties in both osteopenic and healthy rodents. These results indicate that osteoblast-specific aptamer-functionalized LNPs could act as a new RNAi-based bone anabolic strategy, advancing the targeted delivery selectivity of osteogenic siRNAs from the tissue level to the cellular level., Metabolic skeletal disorders associated with impaired bone formation (for example, osteoporosis) remain major clinical challenges. RNA interference (RNAi)-based approaches aimed at promoting osteoblastic bone formation may hold therapeutic potential (1,2). [...]

Published
2015
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11. Molecular Identification of Potential Probiotic Lactic Acid Bacteria Strains Isolated from Egyptian Traditional Fermented Dairy Products

Author

Tang Jijun, Xie Jianwei, Shen Xiaoshu, and Shao Ningsheng

Subjects
0106 biological sciences, 0301 basic medicine, chemistry.chemical_classification, biology, Oligonucleotide, Aptamer, 030106 microbiology, biology.organism_classification, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Lactic acid, enzymes and coenzymes (carbohydrates), 03 medical and health sciences, chemistry.chemical_compound, Ricin, chemistry, Biochemistry, 010608 biotechnology, Fermentation, Nucleotide, Food science, Systematic evolution of ligands by exponential enrichment, Bacteria
Abstract

In order to obtain oligonucleotide aptamers which can specifically recognize the cytotoxin ricin protein,we designed and synthesized an 87 nucleotides ssDNA pool containing 40 random nucleotides flanked by invariant binding regions for PCR primers,which could be used to screen out a specific aptamer for ricin by SELEX (systematic evolution of ligands by exponential enrichment).After several rounds selection,the 38.5% binding ratio of the ssDNA pool bound to ricin protein can be obtained.The results show that the high efficiency of enrichment has been observed by using affinity resin as separation medium.

Published
2015

12. d-/l-Isothymidine incorporation in the core sequence of aptamer BC15 enhanced its binding affinity to the hnRNP A1 protein

Author

Li, Liyu, primary, Yang, Xiantao, additional, Li, Kunfeng, additional, Zhang, Guangpu, additional, Ma, Yuan, additional, Cai, Baobin, additional, Li, Shaohua, additional, Ding, Hongmei, additional, Deng, Jiali, additional, Nan, Xiyan, additional, Sun, Jing, additional, Wu, Yun, additional, Shao, Ningsheng, additional, Zhang, Lihe, additional, and Yang, Zhenjun, additional

Published
2018
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13. Identification of Functional mimotopes of human Vasorin Ectodomain by Biopanning

Author

Li, Da, primary, Zhang, Tan, additional, Yang, Xiqin, additional, Geng, Jie, additional, Li, Shaohua, additional, Ding, Hongmei, additional, Li, Hui, additional, Huang, Aixue, additional, Wang, Chaonan, additional, Sun, Leqiao, additional, Bai, Chenjun, additional, Zhang, Heqiu, additional, Li, Jie, additional, Dong, Jie, additional, and Shao, Ningsheng, additional

Published
2018
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14. Epitope Binning Assay Using an Electron Transfer-Modulated Aptamer Sensor

Author

Li, Min, primary, Guo, Xudong, additional, Li, Hui, additional, Zuo, Xiaolei, additional, Hao, Rongzhang, additional, Song, Hongbin, additional, Aldalbahi, Ali, additional, Ge, Zhilei, additional, Li, Jiang, additional, Li, Qian, additional, Song, Shiping, additional, Li, Shaohua, additional, Shao, Ningsheng, additional, Fan, Chunhai, additional, and Wang, Lihua, additional

Published
2017
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16. An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells

Author

Li, Jie, primary, Dong, Jie, additional, Li, Shaohua, additional, Xia, Wei, additional, Su, Xueting, additional, Qin, Xingliang, additional, Chen, Ying, additional, Ding, Hongmei, additional, Li, Hui, additional, Huang, Aixue, additional, Bai, Chenjun, additional, Hu, Tongnan, additional, Wang, Chenglong, additional, Chu, Bingfeng, additional, and Shao, Ningsheng, additional

Published
2017
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20. SELEX Aptamer Used as a Probe to Detect Circulating Tumor Cells in Peripheral Blood of Pancreatic Cancer Patients

Author

Zhang, Jinqiang, Li, Shaohua, Liu, Fang, Zhou, Lanping, Shao, Ningsheng, and Zhao, Xiaohang

Abstract

Many studies have shown that the quantity and dynamics of circulating tumor cells (CTCs) in peripheral blood of patients afflicted with solid tumours have great relevance in therapeutic efficacy and prognosis. Different methods based on various strategies have been developed to isolate and identify CTCs, but their efficacy needs to be improved because of the rarity and complexity of CTCs. This study was designed to examine the possibility of using a SELEX aptamer (BC-15) as a probe to identify rare CTCs out of nucleated cells. Aptamer BC-15 was selected from a random oligonucleotide library screened against human breast cancer tissue. Fluorescence staining showed that BC-15 had a high affinity for nuclei of human cancer cell lines of various origins as well as CTCs isolated from pancreatic cancer patients, whereas its binding capacity for non-tumor breast epithelial cells and leukocytes was almost undetectable. BC-15+/CD45- cells in cancer patient blood were also found to be cytokeratins 18-positive and aneuploid by immunofluorescence staining and fluorescent in situ hybridization, respectively. Finally, the aptamer method was compared with the well-established anti-cytokeratin method using 15 pancreatic cancer patient blood samples, and enumeration indicated no difference between these two methods. Our study establishes a novel way to identify CTCs by using a synthetic aptamer probe. This new approach is comparable with the anti-cytokeratin-based CTC identification method.

Published
2015

23. Species-specific mutual regulation of p53 and miR-138 between human, rat and mouse

Author

Li, Jie, primary, Xia, Wei, additional, Su, Xueting, additional, Qin, Xingliang, additional, Chen, Ying, additional, Li, Shaohua, additional, Dong, Jie, additional, Ding, Hongmei, additional, Li, Hui, additional, Huang, Aixue, additional, Ge, Xingfeng, additional, Hou, Lvbin, additional, Wang, Chaonan, additional, Sun, Leqiao, additional, Bai, Chenjun, additional, Shen, Xuelian, additional, Fang, Tao, additional, Liu, Yuanlin, additional, Zhang, Yi, additional, Zhang, Hongru, additional, Zhang, Hongwen, additional, and Shao, Ningsheng, additional

Published
2016
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26. Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation

Author

Li, Hui, primary, Guo, Lei, additional, Huang, Aixue, additional, Xu, Hua, additional, Liu, Xuemei, additional, Ding, Hongmei, additional, Dong, Jie, additional, Li, Jie, additional, Wang, Chaonan, additional, Su, Xueting, additional, Ge, Xingfeng, additional, Sun, Leqiao, additional, Bai, Chenjun, additional, Shen, Xuelian, additional, Fang, Tao, additional, Li, Zhanghua, additional, Zhou, Yong, additional, Zhan, Linsheng, additional, Li, Shaohua, additional, Xie, Jianwei, additional, and Shao, Ningsheng, additional

Published
2015
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28. Vasorin is a potential serum biomarker and drug target of hepatocarcinoma screened by subtractive-EMSA-SELEX to clinic patient serum

Author

Li, Shaohua, primary, Li, Hui, additional, Yang, Xiqin, additional, Wang, Wei, additional, Huang, Aixue, additional, Li, Jie, additional, Qin, Xingliang, additional, Li, Fei, additional, Lu, Guanyi, additional, Ding, Hongmei, additional, Su, Xueting, additional, Hou, Lvbin, additional, Xia, Wei, additional, Shi, Ming, additional, Zhang, Hongwen, additional, Zhao, Qiang, additional, Dong, Jie, additional, Ge, Xingfeng, additional, Sun, Leqiao, additional, Bai, Chenjun, additional, Wang, Chaonan, additional, Shen, Xuelian, additional, Fang, Tao, additional, Wang, Fusheng, additional, Zhang, Heqiu, additional, and Shao, Ningsheng, additional

Published
2015
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30. Exosomal Transfer of Vasorin Expressed in Hepatocellular Carcinoma Cells Promotes Migration of Human Umbilical Vein Endothelial Cells

Author

Huang, Aixue, primary, Dong, Jie, additional, Li, Shaohua, additional, Wang, Chaonan, additional, Ding, Hongmei, additional, Li, Hui, additional, Su, Xueting, additional, Ge, Xingfeng, additional, Sun, Leqiao, additional, Bai, Chenjun, additional, Shen, Xuelian, additional, Fang, Tao, additional, Li, Jie, additional, and Shao, Ningsheng, additional

Published
2015
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31. Epitope Binning Assay Using an Electron Transfer-Modulated Aptamer Sensor

Author

Li, Min, Guo, Xudong, Li, Hui, Zuo, Xiaolei, Hao, Rongzhang, Song, Hongbin, Aldalbahi, Ali, Ge, Zhilei, Li, Jiang, Li, Qian, Song, Shiping, Li, Shaohua, Shao, Ningsheng, Fan, Chunhai, and Wang, Lihua

Abstract

Surface plasmon resonance and quartz crystal microbalance are workhorses of protein–DNA interaction research for over 20 years, providing ways to quantitatively determine the protein–DNA binding. However, the cost, necessary technical expertise, and severe nonspecific adsorption poses barriers to their use. Convenient and effective techniques for the measurement of protein–DNA binding affinity and the epitope binning between DNA and proteins for developing highly sensitive detection platform remain challenging. Here, we develop a binding-induced alteration in electron transfer kinetics of the redox reporter labeled (methylene blue) on DNA aptamer to measure the binding affinity between prostate-specific antigen (PSA) and aptamer. We demonstrate that the binding of PSA to aptamer decreases the electron transfer rate of methylene blue for ∼45%. Further, we identify the best pairwise selection of aptamers for developing sandwich assay by sorting from 10 pairwise modes with the PSA detection limit of 500 ng/mL. Our study provides promising ways to analyze the binding affinity between ligand and receptor and to sort pairwise between aptamers or antibodies for the development of highly sensitive sandwich immunoassays.

Published
2018
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32. Systematic study of cis-antisense miRNAs in animal species reveals miR-3661 to target PPP2CAin human cells

Author

Wang, Jian, Li, Zongcheng, Liu, Bailong, Chen, Guangnan, Shao, Ningsheng, Ying, Xiaomin, and Wang, Ya

Abstract

MicroRNAs (miRNAs) suppress targeting gene expression through blocking translation or triggering mRNA degradation and, in general, act in trans, through a partially complementary interaction with the 3′ untranslated region (3′ UTR) or coding regions of a target gene. Although it has been reported previously that some miRNAs suppress their target genes on the opposite strand with a fully complementary sequence (i.e., natural antisense miRNAs that act in cis), there is no report to systematically study such cis-antisense miRNAs in different animal species. Here we report that cis-antisense miRNAs do exist in different animal species: 48 in Caenorhabditis elegans,17 in Drosophila, 36 in Mus musculus, and 52 in Homo sapiensusing a systematical bioinformatics approach. We show that most of these cis-antisense miRNAs can efficiently reduce the expression levels of their target genes in human cells. We further investigate hsa-miR-3661, one of the predicted cis-antisense miRNAs, in detail and demonstrate that this miRNA directly targets the coding sequence of PPP2CAlocated on the opposite DNA strand and inhibits the PPP2CAexpression. Taken together, these results indicate that cis-antisense miRNAs are conservative and functional in animal species including humans.

Published
2016
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33. Magnetically Assisted Surface-Enhanced Raman Spectroscopy for the Detection of Staphylococcus aureusBased on Aptamer Recognition

Author

Wang, Junfeng, Wu, Xuezhong, Wang, Chongwen, Shao, Ningsheng, Dong, Peitao, Xiao, Rui, and Wang, Shengqi

Abstract

A magnetically assisted surface-enhanced Raman scattering (SERS) biosensor for single-cell detection of S. aureuson the basis of aptamer recognition is reported for the first time. The biosensor consists of two basic elements including a SERS substrate (Ag-coated magnetic nanoparticles, AgMNPs) and a novel SERS tag (AuNR–DTNB@Ag–DTNB core–shell plasmonic NPs or DTNB-labeled inside-and-outside plasmonic NPs, DioPNPs). Uniform, monodisperse, and superparamagnetic AgMNPs with favorable SERS activity and magnetic responsiveness are synthesized by using polymer polyethylenimine. AgMNPs use magnetic enrichment instead of repeated centrifugation to prevent sample sedimentation. DioPNPs are designed and synthesized as a novel SERS tag. The Raman signal of DioPNPs is 10 times stronger than that of the commonly used SERS tag AuNR–DTNB because of the double-layer DTNB and the LSPR position adjustment to match the given laser excitation wavelength. Consequently, a strong SERS enhancement is achieved. Under the optimized aptamer density and linker length, capture by aptamer-modified AgMNPs can achieve favorable bacteria arrest (up to 75%). With the conventional Raman spectroscopy, the limit of detection (LOD) is 10 cells/mL for S. aureusdetection, and a good linear relationship is also observed between the SERS intensity at Raman peak 1331 cm–1and the logarithm of bacteria concentrations ranging from 101to 105cells/mL. With the help of the newly developed SERS mapping technique, single-cell detection of S. aureusis easily achieved.

Published
2015
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35. Effects of altered CXCL12/CXCR4 axis on BMP2/Smad/Runx2/Osterix axis and osteogenic gene expressions during osteogenic differentiation of MSCs.

Author

Li Z, Wang W, Xu H, Ning Y, Fang W, Liao W, Zou J, Yang Y, and Shao N

Abstract

This study investigated the effects of altered CXCL12/CXCR4 axis on the bone morphogenetic protein 2 (BMP-2)/Smad/runt-related transcription factor 2 (Runx2)/Osterix (Osx) signal axis and osteogenic gene expression during osteogenic differentiation of mesenchymal stem cells (MSCs), to gain understanding of the link between migration and osteogenic differentiation signal axis and MSCs osteogenic differentiation mechanisms. The pHBAd-MCMV- CXCL12-GFP vector (Ad-CXCL12) was constructed and quantitative polymerase chain reaction (qPCR)/western blotting used to determine CXCL12 expression in Ad-CXCL12-transfected MSCs. MSCs were treated with Ad-CXCL12 and AMD3100 (CXCL12 inhibitor) to detect BMP-2/Smad/Runx2/Osterix expression, bone sialoprotein (BSP), osteocalcin (OCN) and osteopontin (OPN) mRNA expression, and alkaline phosphatase (ALP) activity. PCR and sequencing confirmed successful construction of Ad-CXCL12. qPCR and enzyme-linked immunosorbent assay indicated that Ad-CXCL12 transfection promoted CXCL12 expression in MSCs. At 72 hours, Runx2 and Osterix, and Smad1/5/8 mRNA and protein expressions were significantly higher in the Ad-CXCL12 group than in the control group ( P < 0.01). At 1 and 2 weeks, ALP activity and BSP mRNA expression were significantly higher in the Ad-CXCL12 group than in the control group ( P < 0.01), respectively. No significant difference in OCN and OPN mRNA expression was determined between Ad-CXCL12 and control groups ( P > 0.05). At 3 weeks, no significant difference in mineralized nodule staining was observed between groups ( P > 0.05). Changes in the CXCL12/CXCR4 migration axis affected the BMP-2/Smad/Runx2/Osterix axis and BSP , OCN and OPN mRNA expression in early-stage, but not mid-/latestage, MSCs osteogenic differentiation, therefore affecting the ability of MSCs to undergo osteogenic differentiation., Competing Interests: None.

Published
2017

36. Systematic study of cis-antisense miRNAs in animal species reveals miR-3661 to target PPP2CA in human cells.

Author

Wang J, Li Z, Liu B, Chen G, Shao N, Ying X, and Wang Y

Subjects
Animals, Humans, Phosphorylation, Species Specificity, MicroRNAs metabolism, Protein Phosphatase 2 metabolism, RNA, Antisense metabolism
Abstract

MicroRNAs (miRNAs) suppress targeting gene expression through blocking translation or triggering mRNA degradation and, in general, act in trans, through a partially complementary interaction with the 3' untranslated region (3' UTR) or coding regions of a target gene. Although it has been reported previously that some miRNAs suppress their target genes on the opposite strand with a fully complementary sequence (i.e., natural antisense miRNAs that act in cis), there is no report to systematically study such cis-antisense miRNAs in different animal species. Here we report that cis-antisense miRNAs do exist in different animal species: 48 in Caenorhabditis elegans, 17 in Drosophila, 36 in Mus musculus, and 52 in Homo sapiens using a systematical bioinformatics approach. We show that most of these cis-antisense miRNAs can efficiently reduce the expression levels of their target genes in human cells. We further investigate hsa-miR-3661, one of the predicted cis-antisense miRNAs, in detail and demonstrate that this miRNA directly targets the coding sequence of PPP2CA located on the opposite DNA strand and inhibits the PPP2CA expression. Taken together, these results indicate that cis-antisense miRNAs are conservative and functional in animal species including humans., (© 2015 Wang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)

Published
2016
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