1. Expression of AQP-10, -11 and -12 in the rat stria vascularis.
- Author
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Ichikawa, Rie, Takeda, Taizo, Kakigi, Akinobu, Ito, Hiroaki, Kobayashi, Taisuke, and Hyodo, Masamitsu
- Subjects
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GENE expression , *RATS , *IMMUNOHISTOCHEMISTRY , *ANIMAL experimentation , *MOLECULAR biology , *MEMBRANE proteins , *COCHLEA , *CELLS - Abstract
Water homeostasis is essential for inner ear function. Several aquaporins (AQPs), which are water transport proteins in the cell or plasma membrane, have been reported in the lateral wall of the rat inner ear (cochlea). However, the presence of AQP-10, -11 and -12 has not been reported in the rat stria vascularis (SV) to date. We have aimed to clarify the expression of AQP-10, -11 and -12 in the cochlea lateral wall. Using Wistar rats, we examined the expression of AQP-10, -11 and -12 in the cochlea lateral wall using molecular approaches and immunohistochemistry. AQP-11 was molecular biologically expressed, but the expression of AQP-10 and -12 was not observed. Immunohistochemically, AQP-11 was diffusely localized in the basal cells and marginal cells of the rat SV but was not expressed at the apical site of marginal cells with double staining. The expression of AQP-10 and -12 was not observed. Only AQP-11 was expressed in the basal cells and marginal cells, but it was not expressed at the apical site of marginal cells. Based on this study, AQP-11 may not have an important role in water flux between the perilymph and endolymph. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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