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Hundreds of zoo-based or wildlife webcams have become available during the past twenty years, mostly with the goal of educating the public. However, there has been virtually no peer-reviewed research that evaluates the education, conservation, or scientific impact of webcams. Here, we provide one of the few examples of a webcam used for citizen science, and the only test of efficacy for crowd-sourced data collection using webcams. The Peregrine Fund streamed six seasons of American Kestrel (Falco sparverius) nests using the same nest box from 2012 through 2017 and viewers input observations into an online portal. We analyze trends in participant and kestrel behavior and test for sources of bias in this citizen scientist-generated dataset by independently reviewing a subset of recordings to determine accuracy of viewer-logged data. Citizen scientists logged a maximum of approximately 5.25% of all footage, but with an accuracy of 88%. Although number of participants declined yearly, on average, participants became more engaged. Sources of bias were related to people's daily activity periods (i.e., less participation at night) and activity within the nest box (i.e., less participation when there were no birds in the box). This citizen scientist-generated dataset generally corroborated the literature regarding American Kestrel biology. Researchers may be cautiously optimistic that datasets generated by citizen scientists can provide valuable information on a given system or study species. Given the ubiquity of webcams and their potential competition for conservation dollars, more research evaluating any aspect of their impact or application is sorely needed. Keywords: Webcam, Nest cam, Wildlife, Citizen science, American Kestrel, Falco sparverius

Housing satisfaction is a multidimensional phenomenon that is affected by several factors. The aim of this study is to estimate the factors that affect housing satisfaction, including demographic factors such as household type, age, income, duration of residence, ownership of a house, and education. The physical features factors include the number of bedrooms and the quality of housing units. And the neighborhood facilities factors, including access to quality schools, quality of streets and roads, accessibility to public transportation, community and shopping facilities, and the physical environment. Examining how these factors affect housing satisfaction in urban and rural areas is the study's major goal. The questionnaire consists of three parts: the first part collects demographic characteristics; the second part measures the level of residents' satisfaction with the physical features; the third part measures the level of residents' satisfaction with the neighborhood environment. 580 of the participants responded to the questionnaire. The participants of the study were from three communities: Mafarq City, Manshiyah, and Irhab, which administratively follow Mafraq governorate, Jordan. Manshiyah, and Irhab are considered rural communities. The results show that the level of housing satisfaction among residents in the city and the rural community is approximately the same, as most of the residents in the three communities share the same demographic characteristics, and they are satisfied with their houses, even though they are not satisfied with the neighborhood environment. The importance of this study comes from its results, since the neighborhood environment and facilities play a crucial role in raising the level of residents' satisfaction in their houses. [ABSTRACT FROM AUTHOR]

Abstract A significant challenge for chimeric antigen receptor (CAR) T cell therapy against glioblastoma (GBM) is its immunosuppressive microenvironment, which is densely populated by protumoral glioma-associated microglia and macrophages (GAMs). Myeloid immune checkpoint therapy targeting the CD47-signal regulatory protein alpha (SIRPα) axis induces GAM phagocytic function, but CD47 blockade monotherapy is associated with toxicity and low bioavailability in solid tumors. In this work, we engineer a CAR T cell against epidermal growth factor receptor variant III (EGFRvIII), constitutively secreting a signal regulatory protein gamma-related protein (SGRP) with high affinity to CD47. Anti-EGFRvIII-SGRP CAR T cells eradicate orthotopic EGFRvIII-mosaic GBM in vivo, promoting GAM-mediated tumor cell phagocytosis. In a subcutaneous CD19+ lymphoma mouse model, anti-CD19-SGRP CAR T cell therapy is superior to conventional anti-CD19 CAR T. Thus, combination of CAR and SGRP eliminates bystander tumor cells in a manner that could overcome main mechanisms of CAR T cell therapy resistance, including immune suppression and antigen escape.

Storage systems and warehouse processing in factories are integral to manufacturing operations and logistics procedures. Proper factory layout, storage locations, and procedures save time and reduce cost and wastes. This study examines a real case scenario of a metal industry and sheet metal factory manufacturing commercial easy-to-install metal racks. We collected data, investigated the current situation of the factory, and specified the difficulties and problems regarding the procedures, storage system, and distribution of machines. Furthermore, several proposed solutions were presented and analyzed using simulation, followed by the selection of the best solution. Then, we applied 5S methodology to improve productivity and reduce waste. The findings reveal that reducing waste and time, enhancing productivity, and better space utilization can be achieved significantly. Moreover, this study indicates the feasibility of the proposed solutions, which can be adopted by similar factories and other industries.

Background: This study aimed to assess mothers' knowledge regarding multivitamin use for their children in primary health care settings. Methods: It is a cross-sectional survey targeting mothers visiting Primary Health Care. A questionnaire with 14 questions was distributed among two major primary care clinics. Sampling was convenient, and randomness was assumed by visit sequence, on a first-come, first-served basis, and those who agreed to participate. Mothers visiting the Pediatric and Well Baby Clinic within the PHC centers for immunization of their children (under 2 years old) may have been disproportionately selected by this sampling method. The data were entered and analyzed using SPSS with univariate analysis on each question and use of chi-square, exploring mothers' reasons and practices of vitamin use. Results: In our survey, most mothers (62.3%) believed that the best age of giving multivitamins to their children is in the 1st year of life. Twenty-five percent (25%) believed giving at 2 years old is best. Forty-five percent (45%) believed that multivitamins are good for both boys and girls, whereas 33.5% believed that only girls needed it, and 21% believed that only boys need multivitamins. Mothers who believed that children need multivitamins in the first year of life were 2.3 times more likely to give multivitamins to their children than mothers who believed that multivitamins are important after the first year of life. Mothers who believed that their children benefit most from multivitamins in the first year of life were 1.85 times more likely to give them multivitamins to help increase their weight and physical development. Conclusion: Mothers who know foods containing vitamins are 3 times more likely to give multivitamins to their children. In this study, there is no significant difference between the opinions of mothers giving and not giving multivitamins to increase appetite for physical development beyond the first year of life or prevent diseases or for children with chronic illnesses. Others knowledgeable about foods containing multivitamins were similar to mothers who had no knowledge of buying and preparing foods at home. [ABSTRACT FROM AUTHOR]

Vitamin D deficiency has emerged as a significant concern in public health due to its potential association with various metabolic disorders. This study aimed to investigate the relationship between serum vitamin D levels and the susceptibility to hyperlipidemia among adults. Using a multi-stage sampling approach, we recruited a cohort of 2072 eligible individuals aged over 18 years. Serum 25-hydroxyvitamin D [25(OH)D] levels were measured alongside glucolipid metabolic markers, and comprehensive demographic and physical data were collected. The cohort exhibited a hyperlipidemia prevalence of 42.18%, with 19.88% demonstrating vitamin D deficiency. Notably, 23.68% of individuals with vitamin D deficiency also presented hyperlipidemia. Statistical analysis revealed a significantly higher prevalence of hyperlipidemia among those with vitamin D deficiency compared to those with sufficient levels (23.68% vs. 17.11%, P < 0.05). After adjusting for various factors including age, geographical region, exercise status, BMI, fasting glucose level, and blood pressure, lower serum 25(OH)D concentrations were found to significantly increase the risk of hyperlipidemia (Odds Ratio [OR] = 1.41; 95% CI: 1.057, 1.885; P < 0.05). Further stratification of the hyperlipidemic cohort revealed that vitamin D deficiency was associated with 1.459- and 1.578-times higher risks for total cholesterol and triglyceride abnormalities, respectively, compared to those with sufficient vitamin D levels. Moreover, each 10 ng/mL decrease in serum vitamin D level corresponded to an increased risk of total cholesterol (OR = 0.82; 95% CI: 0.728, 0.974; P < 0.05) and triglyceride abnormalities (OR = 0.79; 95% CI: 0.678, 0.927; P < 0.05). However, there were no significant differences observed between vitamin D-sufficient and−deficient groups regarding Low-Density Lipoprotein (LDL) and High-Density Lipoprotein (HDL) abnormalities. These findings underscore the potential role of serum vitamin D deficiency as an independent risk factor contributing to the elevated prevalence of hyperlipidemia in the adult population.

Abstract B-cell-maturation-antigen (BCMA)-directed therapies are highly active for multiple myeloma, but infections are emerging as a major challenge. In this retrospective, single-center analysis we evaluated infectious complications after BCMA-targeted chimeric-antigen-receptor T-cell therapy (CAR-T), bispecific-antibodies (BsAb) and antibody-drug-conjugates (ADC). The primary endpoint was severe (grade ≥3) infection incidence. Amongst 256 patients, 92 received CAR-T, 55 BsAb and 109 ADC. The incidence of severe infections was higher with BsAb (40%) than CAR-T (26%) or ADC (8%), including grade 5 infections (7% vs 0% vs 0%, respectively). Comparing T-cell redirecting therapies, the incidence rate of severe infections was significantly lower with CAR-T compared to BsAb at 1-year (incidence-rate-ratio [IRR] = 0.43, 95%CI 0.25−0.76, P = 0.004). During periods of treatment-emergent hypogammaglobulinemia, BsAb recipients had higher infection rates (IRR:2.27, 1.31−3.98, P = 0.004) and time to severe infection (HR 2.04, 1.05–3.96, P = 0.036) than their CAR-T counterparts. During periods of non-neutropenia, CAR-T recipients had a lower risk (HR 0.44, 95%CI 0.21−0.93, P = 0.032) and incidence rate (IRR:0.32, 95% 0.17–0.59, P

Abstract Pregnenolone sulfate is a steroid metabolite of the steroidogenesis precursor, pregnenolone, with similar functional properties, including immunosuppression. We recently reported an elevation in serum levels of pregnenolone sulfate in children with malaria, contributing to an immunosuppressed state. Yet, the molecular mechanisms in which this steroid exerts its immunoregulatory functions are lacking. In this study, we examined the effects of pregnenolone sulfate on T cell viability, proliferation and transcriptome. We observed a pregnenolone sulfate dose-dependent induction of T cell death and reduction in proliferation. RNA sequencing analysis of pregnenolone sulfate-treated T cells for 2 and 24 h revealed the downregulation of pro-inflammatory genes and the upregulation of the steroid nuclear receptor superfamily, NR4A, as early-response genes. We also report a strong activation of the integrated stress response mediated by the upregulation of EIF2AK3. These results contribute to the knowledge on transcriptional regulation driving the immunoregulatory effects of pregnenolone sulfate on T cells.

Background: This study investigated the effects of purple sweet potato anthocyanins (PSPA) in a type 2 diabetes mellitus (T2DM) mouse model. Methods: Sixty-five male mice were randomly divided into one control group and four experimental groups, which were fed with a high-fat diet and intraperitoneally injected with streptozotocin (STZ) to induce T2DM. The model mice were treated with 0 (M), 227.5 (LP), 455 (MP), or 910 (HP) mg/kg PSPA for ten days. ELISA, 16S rRNA sequencing, and hematoxylin and eosin staining were used to assess blood biochemical parameters, gut microbial composition, and liver tissue structure, respectively. Results: The FBG concentration was significantly decreased in the LP (6.32 ± 1.05 mmol/L), MP (6.32 ± 1.05 mmol/L), and HP (5.65 ± 0.83 mmol/L) groups; the glycosylated hemoglobin levels were significantly decreased in the HP group (14.43 ± 7.12 pg/mL) compared with that in the M group (8.08 ± 1.04 mmol/L; 27.20 ± 7.72 pg/mL; P

Abstract Cardiovascular events remain a substantial global health concern, necessitating innovative strategies for prevention. This study aims to assess the potential impact of influenza vaccination on major cardiovascular events. A search of the medical English literature was conducted using PubMed/MEDLINE, EMBASE, and the Cochrane CENTRAL up to 1 August 2023. Meta-analysis and stratified analyses were performed to investigate specific outcomes, including myocardial infarction (MI), cardiovascular death, and stroke. Pooled relative risks (RR) along with their 95% confidence intervals (CI) were calculated to evaluate the associations. A comprehensive analysis was conducted on a total of 9059 patients, with 4529 patients receiving the influenza vaccine and 4530 patients receiving a placebo. Among patients who received the influenza vaccine, a notable reduction in the occurrence of major cardiovascular events was observed, with 517 cases compared to 621 cases in the placebo group (RR 0.70; 95% CI 0.55–0.91). The stratified analysis revealed a decreased risk of MI in vaccinated patients (RR 0.74; 95% CI 0.56–0.97) and a significant reduction in cardiovascular death events (RR 0.67; 95% CI 0.45–0.98). This study provides compelling evidence that influenza vaccination is associated with a decreased risk of major cardiovascular events, particularly myocardial infarction, and cardiovascular death. These findings highlight the potential of influenza vaccination as an adjunctive strategy in cardiovascular disease prevention. Further research and exploration of underlying mechanisms are warranted to elucidate the observed beneficial effects.

Glioblastoma (GBM) harbors a highly immunosuppressive tumor microenvironment (TME) which influences glioma growth. Major efforts have been undertaken to describe the TME on a single-cell level. However, human data on regional differences within the TME remain scarce. Here, we performed high-depth single-cell RNA sequencing (scRNAseq) on paired biopsies from the tumor center, peripheral infiltration zone and blood of five primary GBM patients. Through analysis of >45,000 cells, we revealed a regionally distinct transcription profile of microglia (MG) and monocyte-derived macrophages (MdMs) and an impaired activation signature in the tumor-peripheral cytotoxic-cell compartment. Comparing tumor-infiltrating CD8+ T cells with circulating cells identified CX3CR1high and CX3CR1int CD8+ T cells with effector and memory phenotype, respectively, enriched in blood but absent in the TME. Tumor CD8+ T cells displayed a tissue-resident memory phenotype with dysfunctional features. Our analysis provides a regionally resolved mapping of transcriptional states in GBM-associated leukocytes, serving as an additional asset in the effort towards novel therapeutic strategies to combat this fatal disease.

Introduction: Cadmium (Cd) is a hazardous environmental pollutant present in soil, water, and food. Accumulation of Cd in organisms can cause systematic injury and damage to the kidney. The Masson pine pollen aqueous extract (MPPAE) has attracted increasing attention due to its antioxidant activity and ability to enhance immunity.Methods: In this study, we investigated the potential of MPPAE to protect against Cd-induced kidney damage in rats and the underlying mechanism. The transcriptome and metabolome of rats with Cd-induced kidney damage, following treatment with MPPAE, were explored.Results: The concentrations of superoxide dismutase (SOD) and malondialdehyde (MDA) were both significantly altered after treatment with MPPAE. Furthermore, sequencing and analysis of the transcriptome and metabolome of rats with Cd-induced kidney damage, following treatment with MPPAE, revealed differential expression of numerous genes and metabolites compared with the untreated control rats. These differentially expressed genes (DEGs) included detoxification-related genes such as cytochrome P450 and the transporter. The differentially expressed metabolites (DEMs) included 4-hydroxybenzoic acid, L-ascorbate, and ciliatine. Conjoint transcriptome and metabolome analysis showed that several DEGs were correlated with DEMs.Conclusion: These preliminary findings indicate the potential of MPPAE for the treatment of toxic metal poisoning.

Background: Premature ventricular contraction (PVC) symptoms have a direct relationship with, hospitalization and cardiovascular mortality, and finally, the quality of life of patients. The difference in the quality of life between men and women in different arrhythmias has been reported. In this study, the quality of life of PVC patients was evaluated using the arrhythmia-specific questionnaire in tachycardia and arrhythmia (ASTA) questionnaire Methods: A cross-sectional study was conducted on 200 patients with PVCs who were referred to the heart clinic of Modares Hospital in Tehran in 2019. The demographic characteristics and evaluation of the patient's symptoms were recorded through the ASTA questionnaire. The relationship between the answers to the questionnaire and the gender of the patients was measured with statistical tests. Results: 200 patients with abnormal ventricular contraction (88 females and 112 male PVC) with an average age of 16.4 ± 52.7 years (minimum 25 years and maximum 85 years) were studied. The chi-square test did not show a significant relationship between the questions of the ASTA questionnaire and the gender of the patients. Conclusion: The findings of this study show that quality of life and ASTA questionnaire items in PVC patients have no significant relationship with the gender of this category of patients.

IntroductionIndividuals diagnosed with atherosclerotic cardiovascular disease (ACD) are exposed to an increased risk of cardiovascular events. Reducing low-density lipoprotein cholesterol (LDL-C) levels has been established as an effective approach to mitigate these risks. However, a comprehensive and up-to-date meta-analysis investigating the LDL-C-lowering effectiveness and the impact on coronary atherosclerotic plaque compositions of Ezetimibe has been lacking.MethodsWe conducted a systematic review by meticulously analyzing databases such as MEDLINE, EMBASE, and the Cochrane CENTRAL for randomized controlled trials that evaluated the efficacy of ezetimibe in lowering LDL-C levels and its influence on coronary atherosclerotic plaques among individuals with ACD. This review encompassed studies available until August 1, 2023. In our analysis, we employed the weighted mean difference (WMD) as the aggregated statistical measure, accompanied by the corresponding 95% confidence interval (CI).ResultsWe encompassed a total of 20 eligible studies. Our findings unveiled that the combined therapy involving ezetimibe alongside statins led to a more substantial absolute decrease in LDL-C in comparison to using statins alone. This difference in means amounted to (−14.06 mg/dl; 95% CI −18.0 to −10.0; p = 0.0001). Furthermore, upon conducting subgroup analyses, it became evident that the intervention strategies proved effective in diminishing the volume of dense calcification (DC) in contrast to the control group.ConclusionsOur study findings indicate that the inclusion of ezetimibe in conjunction with statin therapy leads to a modest yet meaningful additional reduction in LDL-C levels when compared to using statins in isolation. Importantly, the introduction of ezetimibe resulted in a significant decrease in the volume of DC. However, it is worth noting that further investigation is warranted to delve deeper into this phenomenon.

Abstract Background Patients with schizophrenia (SCZ) exhibit poorer color discrimination than normal individuals. Although retinal abnormalities, as well as cortical and subcortical alterations, found in patients with SCZ have been suggested to cause this poor color discrimination, the impact of cognitive impairment remains to be determined. Dopamine (DA) and glutamate (Glu), known to be disrupted in SCZ, are also suggested to play a role in color discrimination. Our objective was to investigate the contribution of cognitive impairment to color discrimination deficits in SCZ and to examine if these deficits are correlated to SCZ symptoms. Methods This study includes 127 patients with SCZ between July and September 2021. The participants completed several questionnaires, specifically the Positive and Negative Syndrome Scale (PANSS), the Montreal Cognitive Assessment (MoCA) test, and the Farnsworth D-15 test, to assess the extent of SCZ symptoms, cognition, and color discrimination respectively. Results Higher cognition (Beta = − 0.279) was significantly associated with a lower total error score (TES). Moreover, a higher positive PANSS score (Beta = 0.217) was significantly associated with a higher TES. A multinomial regression analysis taking the type of color blindness as the dependent variable showed that female sex (ORa = 5.46) was significantly associated with a certain type of color blindness. Conclusion Color discrimination deficits in patients with SCZ may be due to the effect of cognitive impairment and/or SCZ itself.