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Your search keyword '"Tilan, Jason"' showing total 26 results
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26 results on '"Tilan, Jason"'

1. Hypoxia-activated neuropeptide Y/Y5 receptor/RhoA pathway triggers chromosomal instability and bone metastasis in Ewing sarcoma

Author

Lu, Congyi, Mahajan, Akanksha, Hong, Sung-Hyeok, Galli, Susana, Zhu, Shiya, Tilan, Jason U., Abualsaud, Nouran, Adnani, Mina, Chung, Stacey, Elmansy, Nada, Rodgers, Jasmine, Rodriguez, Olga, Albanese, Christopher, Wang, Hongkun, Regan, Maureen, Zgonc, Valerie, Blancato, Jan, Krawczyk, Ewa, Gallicano, G. Ian, Girgis, Michael, Cheema, Amrita, Iżycka-Świeszewska, Ewa, Cavalli, Luciane R., Pack, Svetlana D., and Kitlinska, Joanna

Published
2022
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2. Publisher Correction: Hypoxia-activated neuropeptide Y/Y5 receptor/RhoA pathway triggers chromosomal instability and bone metastasis in Ewing sarcoma

Author

Lu, Congyi, Mahajan, Akanksha, Hong, Sung-Hyeok, Galli, Susana, Zhu, Shiya, Tilan, Jason U., Abualsaud, Nouran, Adnani, Mina, Chung, Stacey, Elmansy, Nada, Rodgers, Jasmine, Rodriguez, Olga, Albanese, Christopher, Wang, Hongkun, Regan, Maureen, Zgonc, Valerie, Blancato, Jan, Krawczyk, Ewa, Gallicano, G. Ian, Girgis, Michael, Cheema, Amrita, Iżycka-Świeszewska, Ewa, Cavalli, Luciane R., Pack, Svetlana D., and Kitlinska, Joanna

Published
2022
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8. Abstract PR018: Neuropeptide Y as a metastatic factor

Author

Adnani, Mina, primary, Hong, Sung-Hyeok, additional, Galli, Susana, additional, Mahajan, Akanksha, additional, Abualsaud, Nouran, additional, Caprio, Lindsay, additional, Tilan, Jason U., additional, Rodriguez, Olga, additional, Wang, Hingkun, additional, Albanese, Christopher, additional, Cavalli, Luciane R., additional, and Kitlinska, Joanna, additional

Published
2023
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13. In vivo model for testing effect of hypoxia on tumor metastasis

Author

Hong, Sung-Hyeok, Tilan, Jason U., Galli, Susana, Acree, Rachel, Connors, Katherine, Mahajan, Akanksha, Wietlisbach, Larissa, Polk, Taylor, Izycka-Swieszewska, Ewa, Lee, Yi-Chien, Cavalli, Luciane R., Rodriguez, Olga C., Albanese, Chris, and Kitlinska, Joanna B.

Subjects
Disease Models, Animal, Mice, Animals, Humans, Mice, SCID, Sarcoma, Ewing, Neoplasm Metastasis, Hypoxia, Article, Neoplasm Transplantation
Abstract

Hypoxia has been implicated in the metastasis of Ewing sarcoma (ES) by clinical observations and in vitro data, yet direct evidence for its pro-metastatic effect is lacking and the exact mechanisms of its action are unclear. Here, we report an animal model that allows for direct testing of the effects of tumor hypoxia on ES dissemination and investigation into the underlying pathways involved. This approach combines two well-established experimental strategies, orthotopic xenografting of ES cells and femoral artery ligation (FAL), which induces hindlimb ischemia. Human ES cells were injected into the gastrocnemius muscles of SCID/beige mice and the primary tumors were allowed to grow to a size of 250 mm3. At this stage either the tumors were excised (control group) or the animals were subjected to FAL to create tumor hypoxia, followed by tumor excision 3 days later. The efficiency of FAL was confirmed by a significant increase in binding of hypoxyprobe-1 in the tumor tissue, severe tumor necrosis and complete inhibition of primary tumor growth. Importantly, despite these direct effects of ischemia, an enhanced dissemination of tumor cells from the hypoxic tumors was observed. This experimental strategy enables comparative analysis of the metastatic properties of primary tumors of the same size, yet significantly different levels of hypoxia. It also provides a new platform to further assess the mechanistic basis for the hypoxia-induced alterations that occur during metastatic tumor progression in vivo. In addition, while this model was established using ES cells, we anticipate that this experimental strategy can be used to test the effect of hypoxia in other sarcomas, as well as tumors orthotopically implanted in sites with a well-defined blood supply route.

Published
2016

18. In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

Author

Hong, Sung-Hyeok, primary, Tilan, Jason U., primary, Galli, Susana, primary, Acree, Rachel, primary, Connors, Katherine, primary, Mahajan, Akanksha, primary, Wietlisbach, Larissa, primary, Polk, Taylor, primary, Izycka-Swieszewska, Ewa, primary, Lee, Yi-Chien, primary, Cavalli, Luciane R., primary, Rodriguez, Olga C., primary, Albanese, Chris, primary, and Kitlinska, Joanna B., primary

Published
2016
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19. Abstract 2478: Tumor hypoxia promotes Ewing sarcoma metastases in a mouse xenograft model

Author

Tilan, Jason U., primary, Hong, Sung-Hyeok, additional, Galli, Susana, additional, Acree, Rachel, additional, Connors, Katherine, additional, Horton, Meredith, additional, Mahajan, Akanksha, additional, Wietlisbach, Larissa, additional, Lee, Yi-Chien, additional, Rodriguez, Olga, additional, Albanese, Christopher, additional, and Kitlinska, Joanna, additional

Published
2016
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24. High neuropeptide Y release associates with Ewing sarcoma bone dissemination -in vivomodel of site-specific metastases

Author

Hong, Sung-Hyeok, primary, Tilan, Jason U., additional, Galli, Susana, additional, Izycka-Swieszewska, Ewa, additional, Polk, Taylor, additional, Horton, Meredith, additional, Mahajan, Akanksha, additional, Christian, David, additional, Jenkins, Shari, additional, Acree, Rachel, additional, Connors, Katherine, additional, Ledo, Phuong, additional, Lu, Congyi, additional, Lee, Yi-Chien, additional, Rodriguez, Olga, additional, Toretsky, Jeffrey A., additional, Albanese, Chris, additional, and Kitlinska, Joanna, additional

Published
2015
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25. Systemic levels of neuropeptide Y and dipeptidyl peptidase activity in patients with Ewing sarcoma—Associations with tumor phenotype and survival

Author

Tilan, Jason U., primary, Krailo, Mark, additional, Barkauskas, Donald A., additional, Galli, Susana, additional, Mtaweh, Haifa, additional, Long, Jessica, additional, Wang, Hongkun, additional, Hawkins, Kirsten, additional, Lu, Congyi, additional, Jeha, Dima, additional, Izycka‐Swieszewska, Ewa, additional, Lawlor, Elizabeth R., additional, Toretsky, Jeffrey A., additional, and Kitlinska, Joanna B., additional

Published
2014
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26. High neuropeptide Y release associates with Ewing sarcoma bone dissemination - in vivo model of site-specific metastases.

Author

Hong SH, Tilan JU, Galli S, Izycka-Swieszewska E, Polk T, Horton M, Mahajan A, Christian D, Jenkins S, Acree R, Connors K, Ledo P, Lu C, Lee YC, Rodriguez O, Toretsky JA, Albanese C, and Kitlinska J

Subjects
Animals, Bone Neoplasms pathology, Brain Neoplasms secondary, Cell Line, Tumor, Cell Movement, Culture Media, Conditioned, Disease Models, Animal, Female, Gene Silencing, Humans, Hypoxia, Lung Neoplasms secondary, Mice, Mice, SCID, Neoplasm Metastasis, Neoplasm Transplantation, Oncogene Proteins, Fusion metabolism, Phenotype, Proto-Oncogene Protein c-fli-1 metabolism, RNA-Binding Protein EWS metabolism, Sarcoma, Ewing pathology, Bone Neoplasms metabolism, Neuropeptide Y chemistry, Sarcoma, Ewing metabolism
Abstract

Ewing sarcoma (ES) develops in bones or soft tissues of children and adolescents. The presence of bone metastases is one of the most adverse prognostic factors, yet the mechanisms governing their formation remain unclear. As a transcriptional target of EWS-FLI1, the fusion protein driving ES transformation, neuropeptide Y (NPY) is highly expressed and released from ES tumors. Hypoxia up-regulates NPY and activates its pro-metastatic functions. To test the impact of NPY on ES metastatic pattern, ES cell lines, SK-ES1 and TC71, with high and low peptide release, respectively, were used in an orthotopic xenograft model. ES cells were injected into gastrocnemius muscles of SCID/beige mice, the primary tumors excised, and mice monitored for the presence of metastases. SK-ES1 xenografts resulted in thoracic extra-osseous metastases (67%) and dissemination to bone (50%) and brain (25%), while TC71 tumors metastasized to the lungs (70%). Bone dissemination in SK-ES1 xenografts associated with increased NPY expression in bone metastases and its accumulation in bone invasion areas. The genetic silencing of NPY in SK-ES1 cells reduced bone degradation. Our study supports the role for NPY in ES bone invasion and provides new models for identifying pathways driving ES metastases to specific niches and testing anti-metastatic therapeutics.

Published
2015
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