91 results on '"Uenishi H"'
Search Results
2. Analysis of the expression of porcine CD200R1 and CD200R1L by using newly developed monoclonal antibodies
- Author
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Poderoso, T., Martínez de la Riva, P., Uenishi, H., Alvarez, B., Toki, D., Nieto-Pelegrín, E., Alonso, F., Domínguez, J., Ezquerra, A., and Revilla, C.
- Published
- 2019
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3. Molecular and functional characterization of porcine Siglec-3/CD33 and analysis of its expression in blood and tissues
- Author
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Álvarez, B., Escalona, Z., Uenishi, H., Toki, D., Revilla, C., Yuste, M., del Moral, M. Gómez, Alonso, F., Ezquerra, A., and Domínguez, J.
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- 2015
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4. Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues
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Escalona, Z., Álvarez, B., Uenishi, H., Toki, D., Yuste, M., Revilla, C., del Moral, M. Gómez, Alonso, F., Ezquerra, A., and Domínguez, J.
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- 2015
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5. Community structure of denitrifying and total bacteria during nitrogen accumulation in an ammonia‐loaded biofilter
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Yasuda, T., Waki, M., Fukumoto, Y., Hanajima, D., Kuroda, K., Suzuki, K., Matsumoto, T., and Uenishi, H.
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- 2017
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6. Genome-wide association QTL mapping for teat number in a purebred population of Duroc pigs
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Arakawa, A., Okumura, N., Taniguchi, M., Hayashi, T., Hirose, K., Fukawa, K., Ito, T., Matsumoto, T., Uenishi, H., and Mikawa, S.
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- 2015
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7. Wild boars from Sweden, Austria, the Czech Republic and Japan possess intact mannose-binding lectin 2 (MBL2) genes
- Author
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Bergman, I. M., Okumura, N., Uenishi, H., Hammer, S. E., Knoll, A., Edfors, I., and Juul-Madsen, H. R.
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- 2015
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8. Changes of interleukin-6 after wheelchair basketball games in persons with spinal cord injury
- Author
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Kinoshita, T., primary, Nakamura, T., additional, Nishimura, Y., additional, Umemoto, Y., additional, Kojima, D., additional, Uenishi, H., additional, and Tajima, F., additional
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- 2015
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9. Plasma IL-6 elevation with thoracholumbar spinal cord injury during a wheelchair half-marathon race
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Kojima, D., primary, Sasaki, Y., additional, Nakamura, T., additional, Nishimura, Y., additional, Kinoshita, T., additional, Umemoto, Y., additional, Uenishi, H., additional, and Tajima, F., additional
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- 2015
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10. Evidence of halo structure inMg37observed via reaction cross sections and intruder orbitals beyond the island of inversion
- Author
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Takechi, M., primary, Suzuki, S., additional, Nishimura, D., additional, Fukuda, M., additional, Ohtsubo, T., additional, Nagashima, M., additional, Suzuki, T., additional, Yamaguchi, T., additional, Ozawa, A., additional, Moriguchi, T., additional, Ohishi, H., additional, Sumikama, T., additional, Geissel, H., additional, Aoi, N., additional, Chen, Rui-Jiu, additional, Fang, De-Qing, additional, Fukuda, N., additional, Fukuoka, S., additional, Furuki, H., additional, Inabe, N., additional, Ishibashi, Y., additional, Itoh, T., additional, Izumikawa, T., additional, Kameda, D., additional, Kubo, T., additional, Lantz, M., additional, Lee, C. S., additional, Ma, Yu-Gang, additional, Matsuta, K., additional, Mihara, M., additional, Momota, S., additional, Nagae, D., additional, Nishikiori, R., additional, Niwa, T., additional, Ohnishi, T., additional, Okumura, K., additional, Ohtake, M., additional, Ogura, T., additional, Sakurai, H., additional, Sato, K., additional, Shimbara, Y., additional, Suzuki, H., additional, Takeda, H., additional, Takeuchi, S., additional, Tanaka, K., additional, Tanaka, M., additional, Uenishi, H., additional, Winkler, M., additional, Yanagisawa, Y., additional, Watanabe, S., additional, Minomo, K., additional, Tagami, S., additional, Shimada, M., additional, Kimura, M., additional, Matsumoto, T., additional, Shimizu, Y. R., additional, and Yahiro, M., additional
- Published
- 2014
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11. Evidence of halo structure in ^<37>Mg observed via reaction cross sections and intruder orbitals beyond the island of inversion
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Takechi, M., Suzuki, S., Nishimura, D., Fukuda, M., Ohtsubo, T., Nagashima, M., Suzuki, T., Yamaguchi, T., Ozawa, A., Moriguchi, T., Ohishi, H., Sumikama, T., Geissel, H., Aoi, N., Chen, Rui-Jiu, Fang, De-Qing, Fukuda, N., Fukuoka, S., Furuki, H., Inabe, N., Ishibashi, Y., Itoh, T., Izumikawa, T., Kameda, D., Kubo, T., Lantz, M., Lee, C. S., Ma, Yu-Gang, Matsuta, K., Mihara, M., Momota, S., Nagae, D., Nishikiori, R., Niwa, T., Ohnishi, T., Okumura, K., Ohtake, M., Ogura, T., Sakurai, H., Sato, K., Shimbara, Y., Suzuki, H., Takeda, H., Takeuchi, S., Tanaka, K., Tanaka, M., Uenishi, H., Winkler, M., Yanagisawa, Y., Watanabe, S., Minomo, K., Tagami, S., Shimada, M., Kimura, M., Matsumoto, T., Shimizu, Y. R., and Yahiro, M.
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Nuclear Theory ,Nuclear Experiment - Abstract
Precise reaction cross sections (σR) for ^Mg on C targets at energies around 240 MeV/nucleon have been measured at the Radioactive Isotope Beam Factory at RIKEN. The σR for ^Mg have been measured for the first time. An enhancement of σ_R compared to the systematics for spherical stable nuclei has been observed, especially in the neutron-rich region, which reflects the deformation of those isotopes. In the vicinity of the drip line the σ_R for ^Mg is especially large. It is shown by analysis using a recently developed theoretical method that this prominent enhancement of σ_R for ^Mg should come from the p-orbital halo formation breaking the N=28 shell gap.
- Published
- 2014
12. Establishment and characterization of an immortalized red river hog blood-derived macrophage cell line.
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Takenouchi T, Masujin K, Ikeda R, Haraguchi S, Suzuki S, Uenishi H, Onda E, and Kokuho T
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- Animals, Swine, Coculture Techniques, Cell Line, Transformed, Cell Line, African Swine Fever virology, African Swine Fever immunology, Macrophages immunology, Macrophages virology, Macrophages metabolism, African Swine Fever Virus
- Abstract
Red river hogs (RRHs) ( Potamochoerus porcus ), a wild species of Suidae living in Africa with a major distribution in the Guinean and Congolian forests, are natural reservoirs of African swine fever virus (ASFV) and typically are asymptomatic. Since blood and tissue macrophages of suid animals are target cell lineages of ASFV, RRH-derived macrophages are expected to play an important role in suppressing disease development in infected individuals. In the present study, we successfully isolated RRH-derived blood macrophages using co-culture techniques of RRH blood cells with porcine kidney-derived feeder cells and immortalized them by transferring SV40 large T antigen and porcine telomerase reverse transcriptase genes. The newly established macrophage cell line of the RRH-derived blood cell origin (RZJ/IBM) exhibited an Iba1-, CD172a-, and CD203a-positive typical macrophage-like phenotype and up-regulated the phosphorylation of nuclear factor-κB p65 subunit and p38 mitogen-activated protein kinase in response to the bacterial cell wall components, lipopolysaccharide (LPS) and muramyl dipeptide. In addition, RZJ/IBM cells produced the precursor form of interleukin (IL)-1β and IL-18 upon a stimulation with LPS, leading to the conversion of IL-18, but not IL-1β, into the mature form. Time-lapse live cell imaging with pHrodo dye-conjugated Escherichia coli BioParticles demonstrated the phagocytotic activity of RZJ/IBM cells. It is important to note that RZJ/IBM cells are clearly susceptible to ASFV infection and support viral replication in vitro . Therefore, the RZJ/IBM cell line provides a unique model for investigating the pathogenesis of ASFV., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Takenouchi, Masujin, Ikeda, Haraguchi, Suzuki, Uenishi, Onda and Kokuho.)
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- 2024
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13. Identification of Nonsynonymous SNPs in Immune-Related Genes Associated with Pneumonia Severity in Pigs.
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Shinkai H, Suzuki K, Itoh T, Yoshioka G, Takenouchi T, Kitazawa H, and Uenishi H
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- Swine, Receptors, Pattern Recognition genetics, Receptors, Pattern Recognition immunology, Male, Female, Genotype, Alleles, Severity of Illness Index, Pneumonia genetics, Pneumonia immunology, Pneumonia microbiology, Pneumonia veterinary, Polymorphism, Single Nucleotide, Swine Diseases genetics, Swine Diseases immunology, Swine Diseases microbiology
- Abstract
We previously showed that several polymorphisms in genes encoding pattern recognition receptors that cause amino acid substitutions alter pathogen recognition ability and disease susceptibility in pigs. In this study, we expanded our analysis to a wide range of immune-related genes and investigated polymorphism distribution and its influence on pneumonia in multiple commercial pig populations. Among the polymorphisms in 42 genes causing 634 amino acid substitutions extracted from the swine genome database, 80 in 24 genes were found to have a minor allele frequency of at least 10% in Japanese breeding stock pigs via targeted resequencing. Of these, 62 single nucleotide polymorphisms (SNPs) in 23 genes were successfully genotyped in 862 pigs belonging to four populations with data on pneumonia severity. Association analysis using a generalized linear mixed model revealed that 12 SNPs in nine genes were associated with pneumonia severity. In particular, SNPs in the cellular receptor for immunoglobulin G FCGR2B and the intracellular nucleic acid sensors IFI16 and LRRFIP1 were found to be associated with mycoplasmal pneumonia of swine or porcine pleuropneumonia in multiple populations and may therefore have wide applications in the improvement of disease resistance in pigs. Functional analyses at the cellular and animal levels are required to clarify the mechanisms underlying the effects of these SNPs on disease susceptibility.
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- 2024
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14. Editorial: Pig translational model in immunological research.
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Splichalova A, Smidt H, Uenishi H, and Splichal I
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- Animals, Swine, Humans, Translational Research, Biomedical, Disease Models, Animal
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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- 2024
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15. Enhanced biodegradable polyester film degradation in soil by sequential cooperation of yeast-derived esterase and microbial community.
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Tsuboi S, Hoshino YT, Yamamoto-Tamura K, Uenishi H, Omae N, Morita T, Sameshima-Yamashita Y, Kitamoto H, and Kishimoto-Mo AW
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- Polyesters metabolism, Esterases metabolism, Saccharomyces cerevisiae metabolism, Soil, Plastics metabolism, Biodegradable Plastics metabolism, Microbiota
- Abstract
The degradation of biodegradable plastics poses a significant environmental challenge and requires effective solutions. In this study, an esterase derived from a phyllosphere yeast Pseudozyma antarctica (PaE) enhanced the degradation and mineralization of poly(butylene succinate-co-adipate) (PBSA) film in soil. PaE was found to substitute for esterases from initial degraders and activate sequential esterase production from soil microbes. The PBSA film pretreated with PaE (PBSA-E) rapidly diminished and was mineralized in soil until day 55 with high CO
2 production. Soil with PBSA-E maintained higher esterase activities with enhancement of microbial abundance, whereas soil with inactivated PaE-treated PBSA film (PBSA-inact E) showed gradual degradation and time-lagged esterase activity increases. The fungal genera Arthrobotrys and Tetracladium, as possible contributors to PBSA-film degradation, increased in abundance in soil with PBSA-inact E but were less abundant in soil with PBSA-E. The dominance of the fungal genus Fusarium and the bacterial genera Arthrobacter and Azotobacter in soil with PBSA-E further supported PBSA degradation. Our study highlights the potential of PaE in addressing concerns associated with biodegradable plastic persistence in agricultural and environmental contexts., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2024
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16. A cross-sectional survey of consistent rehabilitation through long-term care insurance in Japan: a questionnaire survey.
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Asaeda M, Mikami Y, Nishimura Y, Shimokawa T, Shinohara H, Kawasaki T, Kouda K, Ogawa T, Okawa H, Uenishi H, Kuroda R, Mikami Y, Tajima F, and Kubo T
- Abstract
There seems to be a lack of consistency of maintenance/community-based rehabilitation through long-term care insurance. We aimed to clarify whether consistent rehabilitation can be performed through long-term care insurance by questionnaires., Materials and Methods: This study was a cross-sectional study in a nationwide survey among rehabilitation staff and care recipients who completed disease-specific rehabilitation and required maintenance/community-based rehabilitation through long-term care insurance. Consistency of rehabilitation was compared using Fisher's exact tests. The concordance of the rehabilitation evaluation and treatment conducted under medical and long-term care insurance was assessed using the κ coefficient., Results: Six hundred questionnaires from care recipients and staff were analyzed. Of the rehabilitation staff, 264 (44%) obtained rehabilitation plans from medical institutions. There was a significant difference between the responses of "referral from the same medical corporation" and "obtaining the rehabilitation plan" by Fisher's exact test (odds ratio: 3.242; P <0.001). Most rehabilitation treatments under medical insurance comprised walking or training with parallel rods/canes [498 patients (83%)], and 454 patients (76%) received stretching and range-of-motion training for the limbs and spine for long-term care insurance. Muscle strength evaluation was the most frequently conducted under medical and long-term care insurance [383 (73%) and 487 (83%), respectively]. The concordance of the evaluation and treatment content, except for disease-specific evaluation, was low (κ coefficient≤0.6)., Conclusions: The rate of provision of rehabilitation plans was low, and evaluation and treatment content under medical and long-term care insurance was inconsistent. Our results draw attention to the need for consistent rehabilitation plans between disease-specific and maintenance/community-based rehabilitation., Competing Interests: Y.N. received the Ministry of Health, Labour and Welfare, Health Labour Sciences Research Grant (20GA1002). The remaining authors declare that they have no financial conflict of interest with regard to the content of this report.Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article., (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2023
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17. Genome-wide detection of changes in allelic frequency in Landrace pigs selected for resistance to mycoplasma pneumonia of swine.
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Uemoto Y, Ichinoseki K, Matsumoto T, Oka N, Takamori H, Kadowaki H, Shibata C, Suzuki E, Okamura T, Aso H, Kitazawa H, Satoh M, Uenishi H, and Suzuki K
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- Swine genetics, Animals, Gene Frequency genetics, Quantitative Trait Loci genetics, Genomics, Phenotype, Polymorphism, Single Nucleotide genetics, Genome-Wide Association Study veterinary, Pneumonia of Swine, Mycoplasmal genetics, Swine Diseases
- Abstract
Closed-pig line breeding could change the genetic structure at a genome-wide scale because of the selection in a pig breeding population. We investigated the changes in population structure among generations at a genome-wide scale and the selected loci across the genome by comparing the observed and expected allele frequency changes in mycoplasma pneumonia of swine (MPS)-selected pigs. Eight hundred and seventy-four Landrace pigs, selected for MPS resistance without reducing average daily gain over five generations, had 37,299 single nucleotide polymorphisms (SNPs) and were used for genomic analyses. Regarding population structure, individuals in the first generation were the most widely distributed and then converged into a specific group, as they were selected over five generations. For allele frequency changes, 96 and 14 SNPs had higher allele frequency changes than the 99.9% and 99.99% thresholds of the expected changes, respectively. These SNPs were evenly spread across the genome, and a few of these selected regions overlapped with previously detected quantitative trait loci for MPS and immune-related traits. Our results indicated that the considerable changes in allele frequency were identified in many regions across the genome by closed-pig line breeding based on estimated breeding value., (© 2023 The Authors. Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science.)
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- 2023
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18. Rehabilitation in the long-term care insurance domain: a scoping review.
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Shinohara H, Mikami Y, Kuroda R, Asaeda M, Kawasaki T, Kouda K, Nishimura Y, Ohkawa H, Uenishi H, Shimokawa T, Mikami Y, Tajima F, and Kubo T
- Abstract
Purpose: Since the enactment of the long-term care insurance (LTCI) act in 2000, the number of LTCI users has increased annually. However, evidence regarding what is being carried out as rehabilitation treatment under LTCI is lacking. In this study, a scoping review was performed to bridge this knowledge gap., Methods: Articles related to rehabilitation in connection with LTCI published between April 2000 and November 2020 were searched for in PubMed, CINAHL, CENTRAL (Cochrane Central Register of Controlled Trials), Ichushi Web Ver.5, and CiNii and randomized controlled trials (RCTs) of rehabilitation provided under LTCI were examined., Results: Of the 15,572 publications identified, 15 RCTs, including rehabilitation treatment by physiatrists and therapists, met the eligibility criteria of our review and were included. The rehabilitation trials in the 15 RCTs varied and included balance training, exercise therapy, cognitive tasks, and activities such as singing and dancing. The results allowed us to focus on three categories: fall prevention, dementia, and theory and tools interventions related to occupational therapy practice., Conclusion: The focal points of attention in the rehabilitation treatment of LTCI were identified. However, the physical function, quality of life, and activities of daily living (ADL) of those who "need support" vary from person to person. Therefore, the consolidation of evidence on rehabilitation treatment of LTCI must be continued., (© 2022. The Author(s).)
- Published
- 2022
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19. Establishment and characterization of the immortalized porcine lung-derived mononuclear phagocyte cell line.
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Takenouchi T, Masujin K, Suzuki S, Haraguchi S, Hiramatsu K, Kokuho T, and Uenishi H
- Abstract
Mononuclear phagocytes (MNP), including monocytes, dendritic cells (DC), and macrophages, play critical roles in innate immunity. MNP are abundant in the lungs and contribute to host defense against airborne agents and pulmonary immune homeostasis. In this study, we isolated porcine lung-derived MNP (PLuM) from primary cultures of parenchymal lung cells and then immortalized them by transferring the SV40 large T antigen gene and porcine telomerase reverse transcriptase gene using lentiviral vectors. The established cell line, immortalized PLuM (IPLuM), expressed DC/macrophage markers; i.e., CD163, CD172a, and major histocompatibility complex class II, whereas they did not express a porcine monocyte-specific marker, CD52. The expression patterns of these cell surface markers indicate that IPLuM originate from the DC/macrophage lineage rather than the monocyte lineage. The bacterial cell wall components muramyl dipeptide and lipopolysaccharide induced the production of the interleukin-1 family of pro-inflammatory cytokines in IPLuM. Phagocytotic activity was also detected by time-lapse fluorescence imaging of live cells when IPLuM were cultured in the presence of pHrodo dye-conjugated E. coli BioParticles. It is worth noting that IPLuM are susceptible to African swine fever virus infection and support the virus' efficient replication in vitro . Taken together, the IPLuM cell line may be a useful model for investigating host-agent interactions in the respiratory microenvironments of the porcine lung., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Takenouchi, Masujin, Suzuki, Haraguchi, Hiramatsu, Kokuho and Uenishi.)
- Published
- 2022
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20. Polymorphisms in Pattern Recognition Receptor Genes Are Associated with Respiratory Disease Severity in Pig Farms.
- Author
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Suzuki K, Shinkai H, Yoshioka G, Matsumoto T, Takenouchi T, Tanaka J, Shimizu M, Kitazawa H, and Uenishi H
- Abstract
Reduced productivity caused by infections, particularly respiratory diseases, is a serious problem in pig farming. We have previously reported polymorphisms in porcine pattern recognition receptor genes affecting molecular functions and demonstrated that the 2197A/C polymorphism in the nucleotide-binding oligomerization domain containing 2 ( NOD2 ) gene influences porcine circovirus 2-induced mortality. Here, we investigated how these polymorphisms affect respiratory disease-induced lesions, using samples from a slaughterhouse dealing with pigs from two farms. Lung lesions were evaluated using two scoring systems, Goodwin (GW) and slaughterhouse pleuritis evaluation system (SPES), to determine the influence of Mycoplasma hyopneumoniae (Mhp) and Actinobacillus pleuropneumoniae (App), respectively. SPES scores were significantly higher when the 1205T allele of Toll-like receptor 5 ( TLR5 -1205T), rather than TLR5 -1205C, was present. On the farm with more severe Mhp invasion, lower GW lesion scores were significantly associated with the presence of the NOD-like receptor family pyrin domain containing 3 ( NLRP3 )-2906G allele; where App invasion was worse, lower SPES scores were significantly associated with the presence of the NOD2 -2197C allele. Combinations of polymorphisms in pattern recognition receptor genes can therefore be utilized for breeding for resistance against respiratory diseases in pigs. DNA markers of these polymorphisms can thus be used to improve productivity by reducing respiratory diseases due to bacterial pathogens in pig livestock.
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- 2022
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21. Asymptomatic gastric anisakiasis detected in gastric cancer screening: A case report.
- Author
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Harada F, Yamamoto K, Takeuchi A, Uenishi H, Tachiya Y, Yamamoto C, Tani Y, Ohnishi M, Takita M, Tanimoto T, Kami M, and Shineha R
- Abstract
Anisakiasis is a parasitic disease caused by Anisakis simplex and has become an emerging zoonosis as preferences for eating raw or undercooked seafood have become more common. Few case reports of asymptomatic anisakiasis have been published to date. A 79-year-old asymptomatic man underwent esophagogastroduodenoscopy (EGD) for gastric cancer screening. The gastroenterologist diagnosed superficial gastritis without any malignant lesions but found an Anisakis larva while reviewing EGD images. The physician performed a second EGD and removed the larva. The patient reported that he ate the flatfish sashimi for dinner on the day before the first EGD. This case indicates the existence of asymptomatic gastric anisakiasis, indicating that anisakiasis incidence may have previously been underestimated., Competing Interests: The authors report there are no competing interests to declare., (© 2022 The Authors.)
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- 2022
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22. Uracil-auxotrophic marker recycling system for multiple gene disruption in Pseudozyma antarctica.
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Sameshima-Yamashita Y, Yarimizu T, Uenishi H, Tanaka T, and Kitamoto H
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- Saccharomyces cerevisiae genetics, Uracil, Basidiomycota, Ustilaginales genetics
- Abstract
The basidiomycetous yeast Pseudozyma antarctica, which has multiple auxotrophic markers, was constructed, without inserting a foreign gene, as the host strain for the introduction of multiple useful genes. P. antarctica was more resistant to ultraviolet (UV) irradiation than the model yeast Saccharomyces cerevisiae, and a Paura3 mutant (C867T) was obtained after 3 min of UV exposure. A uracil-auxotrophic marker (URA3) recycling system developed in ascomycetous yeasts and fungi was applied to the P. antarctica Paura3 strain. The PaLYS12 and PaADE2 loci were disrupted via site-directed homologous recombination of PaURA3 (pop-in), followed by the removal of PaURA3 (pop-out). In the obtained double auxotrophic strain (Palys12Δ, Paura3), PaADE2 was further disrupted, and PaURA3 was removed to obtain the triple auxotrophic strain PGB800 (Paura3, Palys12Δ, Paade2Δ). The whole-genome sequence of the PGB800 strain did not contain foreign genes used for genetic manipulation and disrupted PaADE2 and PaLYS12, and removed PaURA3, as planned., (© The Author(s) 2022. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
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- 2022
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23. Isolation and immortalization of macrophages derived from fetal porcine small intestine and their susceptibility to porcine viral pathogen infections.
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Takenouchi T, Masujin K, Miyazaki A, Suzuki S, Takagi M, Kokuho T, and Uenishi H
- Abstract
Macrophages are a heterogeneous population of cells that are present in all vertebrate tissues. They play a key role in the innate immune system, and thus, in vitro cultures of macrophages provide a valuable model for exploring their tissue-specific functions and interactions with pathogens. Porcine macrophage cultures are often used for the identification and characterization of porcine viral pathogens. Recently, we have developed a simple and efficient method for isolating primary macrophages from the kidneys and livers of swine. Here, we applied this protocol to fetal porcine intestinal tissues and demonstrated that porcine intestinal macrophages (PIM) can be isolated from mixed primary cultures of porcine small intestine-derived cells. Since the proliferative capacity of primary PIM is limited, we attempted to immortalize them by transferring the SV40 large T antigen and porcine telomerase reverse transcriptase genes using lentiviral vectors. Consequently, immortalized PIM (IPIM) were successfully generated and confirmed to retain various features of primary PIM. We further revealed that IPIM are susceptible to infection by the African swine fever virus and the porcine reproductive and respiratory syndrome virus and support their replication. These findings suggest that the IPIM cell line is a useful tool for developing in vitro models that mimic the intestinal mucosal microenvironments of swine, and for studying the interactions between porcine pathogens and host immune cells., (Copyright © 2022 Takenouchi, Masujin, Miyazaki, Suzuki, Takagi, Kokuho and Uenishi.)
- Published
- 2022
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24. Relationship between ultrasonographically low-echoic lesions under the skin, wheelchair sitting time, and interface pressure on ischial region in individuals with chronic spinal cord injury.
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Kawasaki S, Nishimura Y, Kamijo YI, Uenishi H, Nakamura T, Kouda K, Koike Y, Nakamura T, and Tajima F
- Subjects
- Cross-Sectional Studies, Humans, Sitting Position, Pressure Ulcer diagnostic imaging, Pressure Ulcer etiology, Pressure Ulcer prevention & control, Spinal Cord Injuries diagnostic imaging, Spinal Cord Injuries rehabilitation, Wheelchairs
- Abstract
Objective: To determine the relationship between physical findings, wheelchair sitting time, and interface pressure on ischial region in subjects with spinal cord injury (SCI). Design: Cross-sectional study. Setting: Rehabilitation center in Japan. Participants: Manual wheelchair users with chronic SCI ( n = 45). Interventions: Pressure ulcers (PU) were diagnosed by inspection, palpation, and ultrasonography. Self-reports were obtained on wheelchair sitting time and pressure mapping was recorded while the subject was seated on the wheelchair. Outcome measures: Subjects were divided into those with ultrasonographically low-echoic lesions (PU-positive group, n = 11) and no such lesions (PU-negative group, n = 34). Outcome measures included wheelchair sitting time and interface pressure at bilateral ischial regions. Results: Using ultrasonography, 13 low-echoic lesions were identified in 11 subjects of the PU-positive group. The pressure duration was longer and interface pressure was significantly higher in subjects of the PU-positive group compared with those of the PU-negative group ( P < 0.05 and P < 0.001, respectively). Conclusions: This is the first study to evaluate the interrelationship between physical findings, sitting time, and ultrasonographically measured interface pressure on ischial region area in subjects with spinal cord injury. To prevent pressure ulcers, we recommend avoidance of prolonged wheelchair sitting and measures that can reduce the interface pressure. These variables should be carefully tailored to the needs of the individual subjects with SCI.
- Published
- 2021
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25. NOD2 Genotypes Affect the Symptoms and Mortality in the Porcine Circovirus 2-Spreading Pig Population.
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Suzuki K, Shinkai H, Yoshioka G, Matsumoto T, Tanaka J, Hayashi N, Kitazawa H, and Uenishi H
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- Animals, Capsid Proteins genetics, Circovirus genetics, Circovirus immunology, Circovirus pathogenicity, Female, Genetic Predisposition to Disease, Genotype, Host-Pathogen Interactions genetics, Male, Phylogeny, Polymorphism, Single Nucleotide, Swine virology, Swine Diseases genetics, Swine Diseases mortality, Swine Diseases pathology, Swine Diseases transmission, Circoviridae Infections genetics, Circoviridae Infections mortality, Circoviridae Infections pathology, Circoviridae Infections transmission, Disease Resistance genetics, Nod2 Signaling Adaptor Protein genetics, Swine genetics
- Abstract
The nucleotide oligomerization domain (NOD)-like receptor 2 (NOD2) is an intracellular pattern recognition receptor that detects components of peptidoglycans from bacterial cell walls. NOD2 regulates bowel microorganisms, provides resistance against infections such as diarrhea, and reduces the risk of inflammatory bowel diseases in humans and mice. We previously demonstrated that a specific porcine NOD2 polymorphism ( NOD2 -2197A > C) augments the recognition of peptidoglycan components. In this study, the relationships between porcine NOD2 -2197A/C genotypes affecting molecular functions and symptoms in a porcine circovirus 2b (PCV2b)-spreading Duroc pig population were investigated. The NOD2 allele ( NOD2 -2197A) with reduced recognition of the peptidoglycan components augmented the mortality of pigs at the growing stage in the PCV2b-spreading population. Comparison of NOD2 allele frequencies in the piglets before and after invasion of PCV2b indicated that the ratio of NOD2 -2197A decreased in the population after the PCV2b epidemic. This data indicated that functional differences caused by NOD2 -2197 polymorphisms have a marked impact on pig health and livestock productivity. We suggest that NOD2 -2197CC is a PCV2 disease resistant polymorphism, which is useful for selective breeding by reducing mortality and increasing productivity.
- Published
- 2021
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26. A specific promoter-type in ribonuclease L gene is associated with phagocytic activity in pigs.
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Shinkai H, Takahagi Y, Matsumoto T, Toki D, Takenouchi T, Kitani H, Sukegawa S, Suzuki K, and Uenishi H
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- Animals, Gene Expression, Mice, Phenotype, Promoter Regions, Genetic, Swine, Gene Expression Regulation, Macrophages
- Abstract
We have previously generated Large White pigs with high immune competence using a selection strategy based on phagocytic activity (PA), capacity of alternative complement pathway, and antibody response after vaccination against swine erysipelas. In this study, to identify the genetic changes caused by the immune selection pressure, we compared gene expression and polymorphisms in the promoter region between pigs subjected to the immune selection (immune-selected pigs) and those that were not (non-selected pigs). After lipid A stimulation, using a microarray analysis, 37 genes related to immune function and transcription factor activity showed a greater than three-fold difference in expression between macrophages derived from immune-selected and non-selected pigs. We further performed a polymorphic analysis of the promoter region of the differentially expressed genes, and elucidated the predominant promoter-types in the immune-selected and non-selected pigs, respectively, in the genes encoding ribonuclease L (RNASEL), sterile α motif and histidine-aspartate domain containing deoxynucleoside triphosphate triphosphohydrolase 1, signal transducer and activator of transcription 3, and tripartite motif containing 21. Analysis of the association between these promoter genotypes and the immune phenotypes revealed that the immune-selected promoter-type in RNASEL was associated with increased PA and was inherited recessively. Considering that RNASEL has been reported to be involved in antimicrobial immune response of mice, it may be possible to enhance the PA of macrophages and improve disease resistance in pig populations using RNASEL promoter-type as a DNA marker for selection.
- Published
- 2021
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27. Genome-wide association studies for production, respiratory disease, and immune-related traits in Landrace pigs.
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Uemoto Y, Ichinoseki K, Matsumoto T, Oka N, Takamori H, Kadowaki H, Kojima-Shibata C, Suzuki E, Okamura T, Aso H, Kitazawa H, Satoh M, Uenishi H, and Suzuki K
- Subjects
- Animals, Haplotypes, Phenotype, Respiratory Tract Diseases pathology, Swine, Disease Resistance genetics, Genome-Wide Association Study, Immune System metabolism, Polymorphism, Single Nucleotide, Quantitative Trait Loci, Reproduction, Respiratory Tract Diseases genetics
- Abstract
Identification of a quantitative trait locus (QTL) related to a chronic respiratory disease such as Mycoplasmal pneumonia of swine (MPS) and immune-related traits is important for the genetic improvement of disease resistance in pigs. The objective of this study was to detect a novel QTL for a total of 22 production, respiratory disease, and immune-related traits in Landrace pigs. A total of 874 Landrace purebred pigs, which were selected based on MPS resistance, were genotyped using the Illumina PorcineSNP60 BeadChip. We performed single nucleotide polymorphism (SNP)-based and haplotype-based genome-wide association studies (GWAS) to detect a novel QTL and to evaluate the possibility of a pleiotropic QTL for these traits. SNP-based GWAS detected a total of six significant regions in backfat thickness, ratio of granular leucocytes to lymphatic cells, plasma concentration of cortisol at different ages, and complement alternative pathway activity in serum. The significant region detected by haplotype-based GWAS was overlapped across the region detected by SNP-based GWAS. Most of these detected QTL regions were novel regions with some candidate genes located in them. With regard to a pleiotropic QTL among traits, only three of these detected QTL regions overlapped among traits, and many detected regions independently affected the traits., (© 2021. The Author(s).)
- Published
- 2021
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28. Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection.
- Author
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Indo Y, Kitahara S, Tomokiyo M, Araki S, Islam MA, Zhou B, Albarracin L, Miyazaki A, Ikeda-Ohtsubo W, Nochi T, Takenouchi T, Uenishi H, Aso H, Takahashi H, Kurata S, Villena J, and Kitazawa H
- Subjects
- Animal Feed microbiology, Animals, Disease Models, Animal, Enterotoxigenic Escherichia coli immunology, Enterotoxigenic Escherichia coli pathogenicity, Epithelial Cells immunology, Epithelial Cells microbiology, Escherichia coli Infections immunology, Escherichia coli Infections microbiology, Escherichia coli Infections prevention & control, Female, Immunity, Innate, Intestinal Mucosa microbiology, Mice, Poly I-C administration & dosage, Poly I-C immunology, Rotavirus immunology, Rotavirus pathogenicity, Rotavirus Infections immunology, Rotavirus Infections prevention & control, Rotavirus Infections virology, Superinfection immunology, Superinfection microbiology, Superinfection prevention & control, Swine microbiology, Undaria immunology, Weaning, Escherichia coli Infections veterinary, Ligilactobacillus salivarius immunology, Probiotics administration & dosage, Rotavirus Infections veterinary, Superinfection veterinary, Swine immunology
- Abstract
Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Indo, Kitahara, Tomokiyo, Araki, Islam, Zhou, Albarracin, Miyazaki, Ikeda-Ohtsubo, Nochi, Takenouchi, Uenishi, Aso, Takahashi, Kurata, Villena and Kitazawa.)
- Published
- 2021
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29. Susceptibility of immortalized porcine kidney macrophages to porcine reproductive and respiratory syndrome virus-2 infection.
- Author
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Morozumi T, Takenouchi T, Wada E, Uenishi H, and Nishiyama Y
- Subjects
- Animals, Cell Line, Chlorocebus aethiops, Kidney, Swine, Virus Replication, Macrophages, Alveolar, Porcine Reproductive and Respiratory Syndrome, Porcine respiratory and reproductive syndrome virus
- Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) displays restricted tropism to porcine alveolar macrophages in nature. Meanwhile, non-porcine cell lines derived from African green monkey kidney cell lines are permissive to PRRSV, resulting in their widespread use in PRRSV research. Furthermore, genetically modified cell lines expressing receptors targeted by PRRSV have been established. We previously established porcine immortalized kidney-derived macrophages (IPKMs) that maintained typical macrophage function. In the present study, we demonstrated the advantages of IPKMs for PRRSV research. IPKMs expressed receptors for PRRSV such as CD163 and CD169. The efficiency of virus isolation from field biological samples was higher for IPKMs than for MARC-145 cells. Five different clusters of North American type PRRSV were propagated in IPKMs. Four field strains continuously produced progeny viruses during 10 continuous passages. The efficiency of virus isolation from field biological samples and continuous progeny virus production in the sequential passages using IPKMs indicated that these cells are good vessels for PRRSV research., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2021
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30. An Urgent Webinar for Therapists Working in Local Facilities by Physiatrists and Therapists in a Regional Core Hospital during the COVID-19 Pandemic.
- Author
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Kinoshita T, Yoshikawa T, Mikami Y, Hori S, Koike Y, Yamamoto Y, Kamijo YI, Umemoto Y, Kouda K, Uenishi H, and Tajima F
- Abstract
Background: After the emergence of novel coronavirus disease (COVID-19), cluster infections occurred at several rehabilitation facilities in Japan. Because rehabilitation is necessary for deconditioned COVID-19 patients, preventing cluster infections and providing rehabilitation while protecting therapists were also essential in the Wakayama region., Webinar: The rehabilitation medicine department and division of Wakayama Medical University Hospital, which consists of physiatrists and registered therapists, proposed that the Wakayama Physical Therapy Association hold an urgent webinar for therapists in the Wakayama region. The webinar (120 min in duration) comprised an overview of severe acute respiratory syndrome coronavirus 2, the significance of rehabilitation therapy for COVID-19 patients, instruction in personal protective equipment, and case reports on COVID-19 patients from an affiliated university hospital. The webinar was held on May 16, 2020, after only 9 days of preparation. Ninety-six members of 29 facilities in the Wakayama region participated, including therapists who lived far from the university. Moreover, the webinar was implemented at a lower cost than a conventional meeting. An opportunity was provided to share information among participants, speakers, instructors, and a supervisor after the presentations. The overall level of satisfaction of participants after the webinar was high., Details: The rehabilitation medicine department and division of Wakayama Medical University Hospital, which consists of physiatrists and registered therapists, proposed that the Wakayama Physical Therapy Association hold an urgent webinar for therapists in the Wakayama region. The webinar (120 min in duration) comprised an overview of severe acute respiratory syndrome coronavirus 2, the significance of rehabilitation therapy for COVID-19 patients, instruction in personal protective equipment, and case reports on COVID-19 patients from an affiliated university hospital. The webinar was held on May 16, 2020, after only 9 days of preparation. Ninety-six members of 29 facilities in the Wakayama region participated, including therapists who lived far from the university. Moreover, the webinar was implemented at a lower cost than a conventional meeting. An opportunity was provided to share information among participants, speakers, instructors, and a supervisor after the presentations. The overall level of satisfaction of participants after the webinar was high., Conclusion: This webinar for therapists in local facilities was held by physiatrists and therapists at Wakayama Medical University, a regional core hospital, in collaboration with the regional therapy association. The preparation time was only 9 days and the cost was lower than that for a conventional meeting, thereby allowing participants to share information about infection control for COVID-19 in an efficient and cost-effective manner., Competing Interests: CONFLICTS OF INTEREST: Financial disclosure statements have been obtained, and no conflicts of interest have been reported by the authors or by any individuals in control of the content of this article and our previous presentation., (©2021 The Japanese Association of Rehabilitation Medicine.)
- Published
- 2021
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31. Dexamethasone enhances CD163 expression in porcine IPKM immortalized macrophages.
- Author
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Takenouchi T, Morozumi T, Wada E, Suzuki S, Nishiyama Y, Sukegawa S, and Uenishi H
- Subjects
- Animals, Butadienes pharmacology, Cell Line, Transformed, Cell Proliferation drug effects, Imidazoles pharmacology, Macrophages drug effects, Macrophages virology, Nitriles pharmacology, Porcine Reproductive and Respiratory Syndrome pathology, Porcine Reproductive and Respiratory Syndrome virology, Porcine respiratory and reproductive syndrome virus physiology, Pyridines pharmacology, Sialic Acid Binding Ig-like Lectin 1 metabolism, Swine, Antigens, CD metabolism, Antigens, Differentiation, Myelomonocytic metabolism, Dexamethasone pharmacology, Kidney pathology, Macrophages metabolism, Receptors, Cell Surface metabolism
- Abstract
In our previous study, we established a unique porcine macrophage cell line, immortalized porcine kidney-derived macrophages (IPKM). The purpose of the present study was to further elucidate the characteristics of IPKM. CD163 is a scavenger receptor for the hemoglobin-haptoglobin complex and is used as a phenotypic marker of anti-inflammatory M2 macrophages. The expression of CD163 is enhanced by dexamethasone (DEX), a potent steroidal anti-inflammatory drug, in human and rodent macrophages in vitro. Therefore, we investigated the effects of DEX on CD163 expression in porcine IPKM. Treatment with DEX markedly enhanced CD163 expression in the IPKM. In addition, we found that SB203580, a selective inhibitor of p38 mitogen-activated protein kinase (MAPK), blocked the effects of DEX, suggesting that the p38 MAPK signaling pathway is involved in the regulation of the DEX-induced enhancement of CD163 expression. Since CD163 is considered to be a putative receptor for the porcine reproductive and respiratory syndrome virus (PRRSV), the effects of DEX on the infection of IPKM by PRRSV were evaluated. Although the IPKM were susceptible to infection by the Fostera PRRSV vaccine strain, DEX treatment did not affect the propagation of the virus in the IPKM. This suggests that the DEX-induced enhancement of CD163 expression alone is not sufficient to facilitate the infection of IPKM by PRRSV.
- Published
- 2021
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32. Porcine CLEC12B is expressed on alveolar macrophages and blood dendritic cells.
- Author
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Nieto-Pelegrín E, Álvarez B, Martínez de la Riva P, Toki D, Poderoso T, Revilla C, Uenishi H, Ezquerra A, and Domínguez J
- Subjects
- Animals, Antibodies, Monoclonal immunology, Blood Circulation, Cells, Cultured, Humans, Interleukin-8 metabolism, Lectins, C-Type genetics, Lipopolysaccharides immunology, Macrophage Activation, Receptors, Mitogen genetics, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins immunology, Tumor Necrosis Factor-alpha metabolism, Dendritic Cells immunology, Lectins, C-Type immunology, Macrophages, Alveolar immunology, Receptors, Mitogen immunology, Swine immunology
- Abstract
CLEC12B is a C-type lectin-like receptor expressed on myeloid cells. In this study, we have characterized the porcine homologue of CLEC12B (poCLEC12B). To this end, we have generated constructs encoding a c-myc tagged version of the whole receptor, or its ectodomain fused to the Fc portion of human IgG1, from a cDNA clone obtained from an alveolar macrophage library, and raised monoclonal antibodies (mAb) against this molecule. Using these mAbs, poCLEC12B was found to be expressed on alveolar macrophages and, at lower levels, on blood conventional type 1 dendritic cells (cDC1) and plasmacytoid DCs. No binding was detected on monocytes, monocyte-derived macrophages or monocyte-derived DCs. Engagement of CLEC12B on alveolar macrophages with mAbs had no apparent effect on cytokine production (TNF-α, IL-8) induced by LPS. These results provide the basis for future investigations aimed to assess the role of poCLEC12B in different microbial infections and to evaluate its potential in vaccination strategies targeting DCs., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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33. Factors associated with dynamic knee valgus angle during single-leg forward landing in patients after anterior cruciate ligament reconstruction.
- Author
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Asaeda M, Nakamae A, Hirata K, Kono Y, Uenishi H, and Adachi N
- Abstract
Background: A few studies have reported on how to predict increased dynamic knee valgus angle (KVA), a risk factor for second anterior cruciate ligament (ACL) injury after ACL reconstruction. This study aimed to identify the factors with the potential to predict the KVA during single-leg hop landing., Methods: Using three-dimensional motion analysis systems, knee motion during a single-leg hop landing task was measured in 22 patients who had undergone ACL reconstruction at 8-10 months postoperatively. The KVA at initial contact (IC) and maximum KVA during the 40-ms period after IC were calculated using the point cluster technique; correlations between the KVA and other factors were assessed. We performed multiple regression analysis to determine whether KVA could be predicted by these parameters., Results: The KVA was significantly negatively correlated with the static femorotibial angle (FTA; P < 0.01) and patient height ( P < 0.01). It was positively correlated with the body mass index ( P < 0.05). Multiple regression analysis showed that a small FTA could predict the KVA at IC (β: 0.52, 95% confidence interval (CI): 2.24-(-0.42); P < 0.01). The maximum KVA during the 40-ms period after IC was associated with the FTA (β: 0.46, 95% CI: 2.22-(-0.26); P = 0.02) and height (β: 0.40, 95% CI: 0.59-(-0.02); P = 0.04)., Conclusion: At 8-10 months after ACL reconstruction, the KVA was significantly correlated with the FTA, with reduced FTA being associated with an increased dynamic KVA during single-leg hop landing. The measurement of anatomical parameters may aid in predicting the second ACL injury risk after reconstruction., Competing Interests: The authors have no conflicts of interest relevant to this article., (© 2020 Asia Pacific Knee, Arthroscopy and Sports Medicine Society. Published by Elsevier (Singapore) Pte Ltd.)
- Published
- 2020
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34. Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets.
- Author
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Álvarez B, Nieto-Pelegrín E, Martínez de la Riva P, Toki D, Poderoso T, Revilla C, Uenishi H, Ezquerra A, and Domínguez J
- Subjects
- Animals, Antibodies, Monoclonal isolation & purification, CHO Cells, Cloning, Molecular, Cricetulus, Lectins, C-Type genetics, Lectins, C-Type immunology, Molecular Targeted Therapy, Oligodeoxyribonucleotides genetics, Poly I-C immunology, Recombinant Fusion Proteins genetics, Swine, Toll-Like Receptor 9 agonists, Toll-Like Receptor 9 genetics, Transcriptome, Antibodies, Monoclonal metabolism, Dendritic Cells immunology, Lectins, C-Type metabolism, Leukocytes, Mononuclear metabolism, Macrophages metabolism
- Abstract
CLEC12A has been proposed as a suitable target for delivering antigen to dendritic cells (DCs) to enhance vaccine efficacy both in human and mouse. In this study, we have characterized the porcine homolog of CLEC12A (poCLEC12A). Using new monoclonal antibodies (mAb), raised against its ectodomain, poCLEC12A was found to be expressed on alveolar macrophages, blood conventional type 1 and type 2 DCs and plasmacytoid DCs, but not on monocytes, T cells, B cells or NK cells, in contrast to its human and murine homologs. Western blot analysis showed that in alveolar macrophages this receptor is expressed both as a monomer and a dimer. After binding to DCs, anti- poCLEC12A mAb was efficiently internalized. No significant changes were observed in TNFα or IFNα secretion by plasmacytoid DCs stimulated with either CpGs (ODN2216) or polyinosinic-polycytidylic acid (poly I:C), upon incubation with mAb. These results provide the basis for future investigations aimed to assess the ability of anti-poCLEC12A mAbs to improve vaccine efficacy by targeting antigen to DCs., (Copyright © 2020 Álvarez, Nieto-Pelegrín, Martínez de la Riva, Toki, Poderoso, Revilla, Uenishi, Ezquerra and Domínguez.)
- Published
- 2020
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35. Effect of coronary artery bypass grafting on blood pressure response to head-up tilting.
- Author
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Hori S, Kamijo YI, Yuzaki M, Kawabe T, Minami K, Umemoto Y, Yokoyama M, Uenishi H, Nishimura Y, Kouda K, Mikami Y, and Tajima F
- Subjects
- Aged, Blood Pressure, Case-Control Studies, Cross-Sectional Studies, Echocardiography methods, Female, Humans, Male, Middle Aged, Tilt-Table Test, Cardiac Output physiology, Coronary Artery Bypass methods, Coronary Artery Disease physiopathology, Heart Rate physiology, Posture physiology, Stroke Volume physiology
- Abstract
Blood pressure response to head-up tilt (HUT) in 7 healthy subjects and 9 patients before and after coronary artery bypass grafting (CABG) was measured during supine and 15-min 60° HUT. Stroke volume (SV) and ejection fraction (EF) were assessed by echocardiography. Baseline mean arterial pressure (MAP) and heart rate (HR) in patients before CABG were similar to healthy subjects. MAP in patients decreased by 6 (4-9) mmHg [median (1st-3rd quartiles)] during 7-12 mmHg of HUT with decreased cardiac output (CO = SV × HR) while HR remained unchanged. MAP in healthy subjects remained unchanged during HUT with increased HR. Body weight decreased by 3.5 (2.5-3.7) kg and MAP decreased by 6 (2-13) mmHg during the last 3-min HUT while HR increased after CABG. Decreases in SV and CO during HUT disappeared after CABG. Blood pressure decreased during HUT in patients before and after CABG regardless of HR response.
- Published
- 2020
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36. Full-scale simultaneous partial nitrification, anammox, and denitrification process for treating swine wastewater.
- Author
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Ishimoto C, Sugiyama T, Matsumoto T, Uenishi H, Fukumoto Y, and Waki M
- Subjects
- Animals, Bioreactors, Nitrogen, Oxidation-Reduction, Sewage, Swine, Wastewater, Denitrification, Nitrification
- Abstract
A full-scale swine-wastewater activated sludge treatment plant that contains naturally enriched anammox biofilms was investigated for 2 years. Red biofilm in this system included Planctomycetes at a maximum of 62.5% of the total bacteria diversity, including Candidatus Jettenia and Candidatus Brocadia. The plant was operated with an influent containing 1,104 ± 513 mg/L biochemical oxygen demand (BOD) and 629 ± 198 mg/L total nitrogen (TN) (BOD/N of 1.78 ± 0.58) at a volumetric BOD loading rate of 0.32 ± 0.12 kg/m
3 /d. Notwithstanding drastically varying influent concentrations, BOD removal efficiency was stable at 95 ± 4%. However, TN removal fluctuated at 75 ± 14%. Dissolved oxygen (DO) concentrations in the aeration tank were 0.06-2.0 mg/L. DO concentration greatly affected nitrogen removal, e.g. when DO was lower than 0.3 mg/L, total inorganic nitrogen removal was 61 ± 14% (≤20 °C), 78 ± 16% (20-30 °C), and 75 ± 12% (≥30 °C), whereas at higher DO concentrations, removal rates were 47 ± 13%, 55 ± 16%, and 68%, respectively. As BOD concentration in the influent was limited compared to nitrogen concentration, nitrogen was likely removed by simultaneous nitrification, anammox, and denitrification (SNAD) under microaerobic conditions. Maintaining low DO concentrations would therefore be a simple method to improve nitrogen removal during SNAD processes for swine-wastewater treatment with fluctuating influent.- Published
- 2020
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37. Effects of thiosulfate addition on ammonia and nitrogen removal in biofilters packed with Oyaishi (pumice tuff).
- Author
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Yasuda T, Fukumoto Y, Waki M, Matsumoto T, and Uenishi H
- Subjects
- Hydrogen-Ion Concentration, Nitrates, Nitrites, Nitrosomonadaceae, RNA, Ribosomal, 16S, Thiobacillus, Air Filters, Ammonia, Denitrification, Nitrogen, Silicates, Thiosulfates, Wastewater
- Abstract
Ammonia removal is achieved partly by absorption and nitrification in biofilters, resulting in the accumulation of nitrogen and the necessity of treating the effluent water. We investigated the effects of thiosulfate addition to a biofilter containing pumice tuff for ammonia and nitrogen removal in a laboratory-scale experiment. The addition of thiosulfate to the circulating water led to a decreased nitrate and nitrite along with an increase of sulfate. The inorganic nitrogen in the circulating water decreased by up to 44% with thiosulfate addition compared to without thiosulfate. Batch experiments revealed that denitrification activity decreased exponentially along with increases in dissolved oxygen; however, approximately 30% of denitrification activity was maintained at dissolved oxygen concentration of 3.3 mg/L. Metabarcoding of 16S rRNA genes indicated that the genus Thiobacillus had a relative abundance of 0.002%-0.016% of total bacteria in the biofilter packing material. The circulating water pH was decreased below 5 with sulfur oxidation, and ammonium was accumulated without pH control resulting in a decrease in the relative abundance of the family Nitrosomonadaceae. Its relative abundance increased with control of pH to near neutral, indicating that ammonia-oxidizing activity could be maintained by adjusting pH. Thiosulfate addition could stimulate nitrogen removal by sulfur-dependent denitrification in biofiltration systems., (© 2019 Japanese Society of Animal Science.)
- Published
- 2020
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38. Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine.
- Author
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He Q, Sawada M, Yamasaki N, Akazawa S, Furuta H, Uenishi H, Meng X, Nakahashi T, Ishigaki Y, and Moriya J
- Subjects
- Animals, Brain drug effects, Brain physiology, Cytokines immunology, Disease Models, Animal, Doublecortin Protein, Fatigue Syndrome, Chronic immunology, Female, Mice, Inbred BALB C, Neurogenesis drug effects, Oxidative Stress drug effects, Anti-Inflammatory Agents therapeutic use, Drugs, Chinese Herbal therapeutic use, Fatigue Syndrome, Chronic drug therapy, Medicine, Kampo
- Abstract
The diagnosis of chronic fatigue syndrome (CFS) is mainly symptom-based, and the etiology is still unclear. Here, we evaluated the pathological changes in the brain of a mouse model of CFS and studied the effects of Kampo medicine. A mouse model of CFS was established through six repeated injections of Brucella abortus (BA) every two weeks for a period of 12 weeks. Neuroinflammation was measured by estimating interleukin (IL)-1β, IL-6, and interferon-gamma (IFN-γ), and oxidative stress by nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) 6 weeks after the last injection. Hippocampal neurogenesis was evaluated through K
i -67, doublecortin (DCX), and 5-bromodeoxyuridine (BrdU) assays. The effects of Kampo medicines (Hochuekkito (TJ-41) and Hachimijiogan (TJ-7)) on neuroinflammation during CFS were studied. The wheel-running activity of mice was decreased by about 50% compared to baseline at 6 weeks after the last BA injection. The levels of IL-1β, IL-6, 3-NT, and 4-HNE were increased in both the cortex and the hippocampus of CFS mice at 6 weeks after the last BA injection. Hippocampal neurogenesis was unchanged in CFS mice. Treatment with TJ-41 and TJ-7 reduced the expressions of IL-1β, IL-6, and IFN-γ in the hippocampus but not in the cortex. The results of the present study indicate that neuroinflammation and oxidative stress play important roles in the pathogenesis of CFS. The data further suggest that treatment with TJ-41 and TJ-7 could help reduce the inflammation associated with CFS in the hippocampus, but failed to improve the symptoms in CFS mice.- Published
- 2020
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39. Paraimmunobiotic Bifidobacteria Modulate the Expression Patterns of Peptidoglycan Recognition Proteins in Porcine Intestinal Epitheliocytes and Antigen Presenting Cells.
- Author
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Iida H, Tohno M, Islam MA, Sato N, Kobayashi H, Albarracin L, Kober AH, Ikeda-Ohtsubo W, Suda Y, Aso H, Nochi T, Miyazaki A, Uenishi H, Iwabuchi N, Xiao JZ, Villena J, and Kitazawa H
- Subjects
- Animals, Antigen-Presenting Cells immunology, Peyer's Patches cytology, Peyer's Patches immunology, Receptors, Pattern Recognition metabolism, Spleen cytology, Spleen immunology, Bifidobacterium physiology, Carrier Proteins metabolism, Intestinal Mucosa immunology, Intestinal Mucosa microbiology, Swine immunology, Swine microbiology
- Abstract
Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer's patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.
- Published
- 2019
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40. Differences in gene expression profiles for subcutaneous adipose, liver, and skeletal muscle tissues between Meishan and Landrace pigs with different backfat thicknesses.
- Author
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Kojima M, Nakajima I, Arakawa A, Mikawa S, Matsumoto T, Uenishi H, Nakamura Y, and Taniguchi M
- Subjects
- Animals, Crosses, Genetic, Female, Gene Expression Regulation, Male, Molecular Sequence Annotation, Adipose Tissue metabolism, Adiposity genetics, Gene Expression Profiling, Liver metabolism, Muscle, Skeletal metabolism, Subcutaneous Tissue metabolism, Swine genetics
- Abstract
Backfat thickness is one of the most important traits of commercially raised pigs. Meishan pigs are renowned for having thicker backfat than Landrace pigs. To examine the genetic factors responsible for the differences, we first produced female crossbred pig lines by mating Landrace (L) × Large White (W) × Duroc (D) females (LWD) with Landrace (L) or Meishan (M) boars (i.e., LWD × L = LWDL for Landrace offspring and LWD × M = LWDM for the Meishan offspring). We confirmed that LWDM pigs indeed had a thicker backfat than LWDL pigs. Next, we performed gene expression microarray analysis in both genetic lines to examine differentially expressed genes (DEGs) in energy metabolism-related tissues, subcutaneous adipose (fat), liver, and longissimus dorsi muscle tissues. We analyzed the annotation of DEGs (2-fold cutoff) to functionally categorize them by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. The number of DEGs in muscle tissues of both lines was much less than that in fat and liver tissues, indicating that DEGs in muscle tissues may not contribute much to differences in backfat thickness. In contrast, several genes related to muscle (in fat tissue) and lipid metabolism (in liver tissue) were more upregulated in LWDM pigs than LWDL pigs, indicating that those DEGs might be responsible for differences in backfat thickness. The different genome-wide gene expression profiles in the fat, liver, and muscle tissues between genetic lines can provide useful information for pig breeders., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2018
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41. Q969R polymorphism in NLRP3 is associated with immune responses to vaccination against bacterial infections in pigs.
- Author
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Shinkai H, Terada K, Toki D, Tohno M, and Uenishi H
- Subjects
- Animals, Female, Interleukin-1beta, Male, Antibody Formation genetics, Bacterial Vaccines immunology, Haemophilus parasuis immunology, NLR Family, Pyrin Domain-Containing 3 Protein genetics, NLR Family, Pyrin Domain-Containing 3 Protein immunology, Polymorphism, Genetic, Receptors, Pattern Recognition genetics, Receptors, Pattern Recognition immunology, Swine immunology, Vaccination
- Abstract
Nucleotide-binding domain, leucine-rich-containing family, pyrin-domain containing-3 (NLRP3) is an important pattern recognition receptor involved in various inflammatory responses and adjuvant effects upon vaccination. We previously identified the Q969R (A2906G) gain-of-function polymorphism in porcine NLRP3, which increased production of interleukin-1β in in vitro gene transfection experiments. Here, we explored the associations between the A2906G polymorphism and antibody responses after vaccination against bacteria in Large White pigs maintained under specific pathogen-free conditions. The NLRP3-2906
A/G pigs had a greater antibody response to vaccine antigens than NLRP3-2906A/A pigs. We observed a significant association of the antibody response against Haemophilus parasuis serotype 2 and 5 with NLRP3 genotypes. As the A2906G polymorphism in NLRP3 is widely distributed in commercial pig breeds, Landrace, Large White and Berkshire pigs, there is potential for improvement in vaccine efficiency and disease resistance using this polymorphism in various pig populations., (© 2018 Japanese Society of Animal Science.)- Published
- 2018
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42. Distribution of the CD4 Alleles in Sus scrofa Demonstrates the Genetic Profiles of Western Breeds and Miniature Pigs.
- Author
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Eguchi-Ogawa T, Matsubara T, Toki D, Okumura N, Ando A, Kitagawa H, and Uenishi H
- Subjects
- Animals, Gene Frequency, Male, Swine, CD4 Antigens genetics, Polymorphism, Single Nucleotide genetics, Sus scrofa genetics, Swine, Miniature genetics
- Abstract
Widely used antipig CD4 monoclonal antibodies (mAbs) fail to recognize CD4 alleles characteristic of miniature pig lines such as the National Institutes of Health (NIH) miniature pigs and microminipigs. We surveyed polymorphisms in the coding sequence of the porcine CD4 gene among Western and Oriental pig breeds and Japanese wild boars and investigated their distribution. Of the 13 alleles that we identified among the 47 animals, 2 in group I and 3 in group II were found exclusively in Western breed pigs. Group IV alleles, which included mAb-nonbinding alleles, were found frequently in Oriental breed pigs, suggesting that the mAb-nonbinding allele arose from the gene pool of Oriental pigs. Group IV alleles were also found in Duroc and Large White pigs, suggesting genetic inflow from Oriental pig breeds into Western breeds. Comparison of the CD4 sequences of species in Cetartiodactyla suggested that the group IV alleles in Sus scrofa occurred before the divergence of this species from the other artiodactyls. The different antibody specificities of the various CD4 alleles may facilitate the discrimination of T-cell populations in transplantation studies using miniature pigs. The significance of the preservation of CD4 polymorphisms to immune function in pigs warrants further investigation.
- Published
- 2018
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43. In vivo effect of a TLR5 SNP (C1205T) on Salmonella enterica serovar Typhimurium infection in weaned, specific pathogen-free Landrace piglets.
- Author
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Muneta Y, Arai N, Yakabe Y, Eguchi M, Shibahara T, Sakuma A, Shinkai H, Uenishi H, Hirose K, and Akiba M
- Subjects
- Animals, Diarrhea immunology, Diarrhea microbiology, Diarrhea veterinary, Feces microbiology, Genotype, Haptoglobins analysis, Interleukin-1beta blood, Lymph Nodes microbiology, Lymph Nodes pathology, Male, Salmonella Infections, Animal immunology, Salmonella Infections, Animal microbiology, Salmonella Infections, Animal pathology, Swine, Swine Diseases microbiology, Weaning, Polymorphism, Single Nucleotide immunology, Salmonella typhimurium immunology, Salmonella typhimurium pathogenicity, Swine Diseases immunology, Toll-Like Receptor 5 immunology
- Abstract
Toll-like receptor 5 is a pattern-recognition receptor for bacterial flagellin. We previously reported that a single nucleotide polymorphism (SNP) of swine TLR5, C1205T, impairs recognition of Salmonella typhimurium (ST) flagellin and ethanol-killed Salmonella Choleraesuis (SC). In the present study, weaned, specific pathogen-free (SPF) Landrace piglets with CC, CT or TT genotypes were orally infected with ST (L-3569 strain) to determine the effect of this specific SNP on ST infection in vivo. Eighteen ST-infected piglets (six each with CC, CT, or TT) exhibited fever and diarrhea for 1 week after infection. TT piglets had the longest duration of fever. TT piglets had the greatest mean diarrhea score during the experimental period, followed by CT and CC piglets. Fecal ST shedding was greater in CT and TT pigs than CC pigs from 2 days after infection. Serum haptoglobin concentration increased in ST-infected piglets and to greater extents in CT and TT pigs than CC pigs. Daily weight gain was lower in infected pigs, particularly TT piglets, than control pigs. To the best of our knowledge, this study is the first to demonstrate that impairment of TLR recognition affects pig susceptibility to disease in vivo. Thus, piglets with the T allele of swine TLR5 (C1205T) exhibit impaired resistance to ST infection. Furthermore, elimination of the T allele of this SNP from Landrace pigs would lead to enhancement of their resistance to ST infection., (© 2018 The Societies and John Wiley & Sons Australia, Ltd.)
- Published
- 2018
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44. Effects of physiatrist and registered therapist operating acute rehabilitation (PROr) in patients with stroke.
- Author
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Kinoshita T, Nishimura Y, Nakamura T, Hashizaki T, Kojima D, Kawanishi M, Uenishi H, Arakawa H, Ogawa T, Kamijo YI, Kawasaki T, and Tajima F
- Subjects
- Aged, Female, Humans, Male, Prospective Studies, Physiatrists, Physical Therapists, Stroke Rehabilitation
- Abstract
Objective: Clinical evidence suggests that early mobilization of patients with acute stroke improves activity of daily living (ADL). The purpose of this study was to compare the utility of the physiatrist and registered therapist operating acute rehabilitation (PROr) applied early or late after acute stroke., Subjects and Methods: This study was prospective cohort study, assessment design. Patients with acute stroke (n = 227) admitted between June 2014 and April 2015 were divided into three groups based on the time of start of PROr: within 24 hours (VEM, n = 47), 24-48 hours (EM, n = 77), and more than 48 hours (OM, n = 103) from stroke onset. All groups were assessed for the number of deaths during hospitalization, and changes in the Glasgow Coma Scale (GCS), National Institute of Health Stroke Scale (NIHSS), and Functional Independence Measure (FIM) at hospital discharge., Interventions: All patients were assessed by physiatrists, who evaluated the specific needs for rehabilitation, and then referred them to registered physical therapists and occupational therapists to provide early mobilization (longer than one hour per day per patient)., Results: The number of deaths encountered during the PROr period was 13 (out of 227, 5.7%), including 2 (4.3%) in the VEM group. GCS improved significantly during the hospital stay in all three groups, but the improvement on discharge was significantly better in the VEM group compared with the EM and OM groups. FIM improved significantly in the three groups, and the gains in total FIM and motor subscale were significantly greater in the VEM than the other groups., Conclusions: PROr seems safe and beneficial rehabilitation to improve ADL in patients with acute stroke.
- Published
- 2017
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45. Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase.
- Author
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Takenouchi T, Kitani H, Suzuki S, Nakai M, Fuchimoto DI, Tsukimoto M, Shinkai H, Sato M, and Uenishi H
- Abstract
The domestic pig is an important agricultural animal, and thus, infectious diseases that affect pigs can cause severe economic losses in the global swine industry. Various porcine pathogens target macrophages, which are classical innate immune cells. Although macrophages basically protect the host from pathogens, they also seem to contribute to infectious processes. Therefore, cultured macrophages can be used to develop in vitro models for studying not only genes associated with porcine innate immunity but also the infectious processes of porcine pathogens. However, the availability of porcine macrophage cell lines is limited. In this study, we describe a novel immortalized porcine kidney-derived macrophage (IPKM) cell line, which was generated by transferring the SV40 large T antigen (SV40LT) and porcine telomerase reverse transcriptase (pTERT) genes into primary porcine kidney-derived macrophages using lentiviral vectors. The IPKM displayed a typical macrophage morphology and was routinely passaged (doubling time: about 4 days). These cells were immunostained for macrophage markers. In addition, they exhibited substantial phagocytosis of polystyrene microbeads and released inflammatory cytokines upon lipopolysaccharide (LPS) stimulation. Furthermore, the maturation and secretion of interleukin-1β were observed after nigericin-induced inflammasome activation in LPS-primed IPKM. These findings suggest that IPKM exhibit the typical inflammatory characteristics of macrophages. By transferring the SV40LT and pTERT genes using lentiviral vectors, we also successfully immortalized macrophages derived from the peripheral blood of a low-density lipoprotein receptor-deficient pig. These results suggest that the co-expression of SV40LT and pTERT is an effective way of immortalizing porcine macrophages.
- Published
- 2017
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46. Noninvasive positive pressure ventilation enhances the effects of aerobic training on cardiopulmonary function.
- Author
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Moriki T, Nakamura T, Kamijo YI, Nishimura Y, Banno M, Kinoshita T, Uenishi H, and Tajima F
- Subjects
- Adult, Heart Function Tests, Humans, Male, Respiratory Function Tests, Stroke Volume, Exercise physiology, Heart physiology, Lung physiology, Noninvasive Ventilation methods, Positive-Pressure Respiration methods
- Abstract
Purpose: The purpose of this study was to determine the effect of aerobic training under noninvasive positive pressure ventilation (NPPV) on maximal oxygen uptake ([Formula: see text])., Methods: Ten healthy young male volunteers participated in the study. Before the training, stroke volume (SV) and cardiac output (CO) were measured in all subjects under 0, 4, 8, and 12 cmH2O NPPV at rest. Then, the subjects exercised on a cycle ergometer at 60% of pre-training [Formula: see text] for 30 min daily for 5 consecutive days with/without NPPV. The 5-day exercise protocol was repeated after a three-week washout period without/with NPPV. The primary endpoint was changes in [Formula: see text]. The secondary endpoints were changes in SV, CO, maximum heart rate (HRmax), maximum respiratory rate (RRmax), maximum expiratory minute volume (VEmax) and the percent change in plasma volume (PV)., Results: NPPV at 12 cmH2O significantly reduced SV and CO at rest. [Formula: see text] significantly increased after 5 days training with and without NPPV, but the magnitude of increase in [Formula: see text] after training under 12 cmH2O NPPV was significantly higher than after training without NPPV. VEmax significantly increased after training under NPPV, but not after training without NPPV. HRmax and RRmax did not change during training irrespective of NPPV. The percent change in PV was similar between training with and without NPPV. The 5-day training program with NPPV resulted in greater improvement in [Formula: see text] than without NPPV., Conclusions: Aerobic training under NPPV has add-on effects on [Formula: see text] and exercise-related health benefits in healthy young men.
- Published
- 2017
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47. Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes.
- Author
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Fukuda T, Tani T, Haraguchi S, Donai K, Nakajima N, Uenishi H, Eitsuka T, Miyagawa M, Song S, Onuma M, Hoshino Y, Sato E, and Honda A
- Subjects
- Animals, Female, Swine, Cellular Reprogramming Techniques, Gene Expression, Induced Pluripotent Stem Cells metabolism, Transcription Factors biosynthesis, X Chromosome metabolism
- Abstract
In this study, we created porcine-induced pluripotent stem (iPS) cells with the expression of six reprogramming factors (Oct3/4, Klf4, Sox2, c-Myc, Lin28, and Nanog). The resulting cells showed growth dependent on LIF (leukemia inhibitory factor) and expression of multiple stem cell markers. Furthermore, the iPS cells caused teratoma formation with three layers of differentiation and had both active X chromosomes (XaXa). Our iPS cells satisfied the both of important characteristics of stem cells: teratoma formation and activation of both X chromosomes. Injection of these iPS cells into morula stage embryos showed that these cells participate in the early stage of porcine embryogenesis. Furthermore, the RNA-Seq analysis detected that expression levels of endogenous pluripotent related genes, NANOG, SOX2, ZFP42, OCT3/4, ESRRB, and ERAS were much higher in iPS with six factors than that with four reprogramming factors. We can conclude that the expression of six reprogramming factors enables the creation of porcine iPS cells, which is partially close to naive iPS state. J. Cell. Biochem. 118: 537-553, 2017. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.)
- Published
- 2017
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48. Erratum to: Pig lacks functional NLRC4 and NAIP genes.
- Author
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Sakuma C, Toki D, Shinkai H, Takenouchi T, Sato M, Kitani H, and Uenishi H
- Published
- 2017
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49. Pig lacks functional NLRC4 and NAIP genes.
- Author
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Sakuma C, Toki D, Shinkai H, Takenouchi T, Sato M, Kitani H, and Uenishi H
- Subjects
- Animals, Cells, Cultured, Inflammasomes immunology, Macrophages immunology, Macrophages metabolism, Swine, Toll-Like Receptors genetics, Toll-Like Receptors metabolism, Bacteria immunology, CARD Signaling Adaptor Proteins genetics, Genome, Immunity, Innate immunology, Inflammasomes genetics, Neuronal Apoptosis-Inhibitory Protein genetics
- Abstract
The NLRC4 inflammasome, which recognizes flagellin and components of the type III secretion system, plays an important role in the clearance of intracellular bacteria. Here, we examined the genomic sequences carrying two genes encoding key components of the NLRC4 inflammasome-NLR family, CARD-containing 4 (NLRC4), and NLR apoptosis inhibitory protein (NAIP)-in pigs. Pigs have a single locus encoding NLRC4 and NAIP. Comparison of the sequences thus obtained with the corresponding regions in humans revealed the deletion of intermediate exons in both pig genes. In addition, the genomic sequences of both pig genes lacked valid open reading frames encoding functional NLRC4 or NAIP protein. Additional pigs representing multiple breeds and wild boars also lacked the exons that we failed to find through genome sequencing. Furthermore, neither the NLRC4 nor the NAIP gene was expressed in pigs. These findings indicate that pigs lack the NLRC4 inflammasome, an important factor involved in monitoring bacterial proteins and contributing to the clearance of intracellular pathogens. These results also suggest that genetic polymorphisms affecting the molecular functions of TLR2, TLR4, TLR5, and other pattern recognition receptors associated with the recognition of bacteria have a more profound influence on disease resistance in pigs than in other species.
- Published
- 2017
- Full Text
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50. Anammox biofilm in activated sludge swine wastewater treatment plants.
- Author
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Suto R, Ishimoto C, Chikyu M, Aihara Y, Matsumoto T, Uenishi H, Yasuda T, Fukumoto Y, and Waki M
- Subjects
- Ammonia chemistry, Ammonia metabolism, Anaerobiosis, Animals, Oxidation-Reduction, Planctomycetales genetics, Planctomycetales growth & development, RNA, Ribosomal, 16S genetics, Sewage microbiology, Swine, Wastewater microbiology, Ammonia analysis, Biofilms growth & development, Bioreactors microbiology, Sewage chemistry, Wastewater chemistry, Water Purification methods
- Abstract
We investigated anammox with a focus on biofilm in 10 wastewater treatment plants (WWTPs) that use activated sludge treatment of swine wastewater. In three plants, we found red biofilms in aeration tanks or final sedimentation tanks. The biofilm had higher anammox 16S rRNA gene copy numbers (up to 1.35 × 10
12 copies/g-VSS) and higher anammox activity (up to 295 μmoL/g-ignition loss/h) than suspended solids in the same tank. Pyrosequencing analysis revealed that Planctomycetes accounted for up to 17.7% of total reads in the biofilm. Most of them were related to Candidatus Brocadia or Ca. Jettenia. The highest copy number and the highest proportion of Planctomycetes were comparable to those of enriched anammox sludge. Thus, swine WWTPs that use activated sludge treatment can fortuitously acquire anammox biofilm. Thus, concentrated anammox can be detected by focusing on red biofilm., (Copyright © 2016 Elsevier Ltd. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF
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