Search

Your search keyword '"Waar K"' showing total 17 results
Start Over Author "Waar K" Publication Year Range Last 10 years
17 results on '"Waar K"'

1. Increase in invasive group A streptococcal (Streptococcus pyogenes) infections (iGAS) in young children in the Netherlands, 2022

Author

de Gier, Brechje, Marchal, Niek, de Beer-Schuurman, Ilse, te Wierik, Margreet, Hooiveld, Mariëtte, de Melker, Hester E., van Sorge, Nina M., Stuart, J. W. T. Cohen, Melles, D. C., van Dijk, K., Alzubaidy, A., Scholing, M., Kuil, S. D., Blaauw, G. J., Altorf-van der Kuil, W., Bierman, S. M., de Greeff, S. C., Groenendijk, S. R., Hertroys, R., Kuijper, E. J., Marchal, N., Monen, J. C. M., Notermans, D. W., Polman, J., van den Reek, W. J., Schneeberger-van der Linden, C., Schoffelen, A. F., Wielders, C. C. H., de Wit, B. J., Zoetigheid, R. E., van den Bijllaardt, W., Kraan, E. M., Haeseker, M. B., da Silva, J. M., de Jong, E., Maraha, B., van Griethuysen, A. J., Wintermans, B. B., van Trijp, M. J. C. A., Wong, M., Muller, A. E., Ott, A., Lokate, M., Bathoorn, E., Sinnige, J., Silvis, W., Renders, N. H., Bakker, L. J., Dorigo-Zetsma, J. W., Waar, K., van der Beek, M. T., Leversteijn-van Hall, M. A., van Mens, S. P., Schaftenaar, E., Nabuurs-Franssen, M. H., Maat, I., Diederen, B. M. W., Bode, L. G. M., Ong, D. S. Y., van Rijn, M., Dinant, S., Pontesilli, O., van Dam, D. W., de Brauwer, E. I. G. B., Bentvelsen, R. G., Buiting, A. G. M., Vlek, A. L. M., de Graaf, M., Troelstra, A., Jansz, A. R., van Meer, M. P. A., de Vries, J., Dos Santos, C. Oliveira, Rümke, Lidewij W., Vestjens, Stefan M. T., Vlaminckx, Bart J. M., Medical Microbiology and Infection Prevention, AII - Infectious diseases, APH - Global Health, APH - Methodology, APH - Quality of Care, Experimental Immunology, AII - Inflammatory diseases, APH - Aging & Later Life, and APH - Health Behaviors & Chronic Diseases

Subjects
Adult, Chickenpox, Streptococcus pyogenes, Child, Preschool, Streptococcal Infections/diagnosis, COVID-19, Humans, Netherlands/epidemiology, Child, Herpes Zoster, Pandemics
Abstract

In 2022, a sevenfold increase in the number of notifiable invasive Streptococcus pyogenes (iGAS) infections among children aged 0-5 years was observed in the Netherlands compared with pre-COVID-19 pandemic years. Of 42 cases in this age group, seven had preceding or coinciding varicella zoster infections, nine were fatal. This increase is not attributable to a specific emm type. Vigilance for clinical deterioration as iGAS sign is warranted in young children, especially those with varicella zoster infection.

Published
2023

2. Third-generation cephalosporin resistant gram-negative bacteraemia in patients with haematological malignancy; an 11-year multi-centre retrospective study

Author

de la Court, Jara R., Woudt, Sjoukje H. S., Schoffelen, Annelot F., Heijmans, Jarom, de Jonge, Nick A., van der Bruggen, Tjomme, Bomers, Marije K., Lambregts, Merel M. C., Schade, Rogier P., Sigaloff, Kim C. E., Stuart, J. W. T. Cohen, Melles, D. C., van Dijk, K., Alzubaidy, A., Werdmuller, B. F. M., Blaauw, G. J., Diederen, B. M. W., Alblas, H. J., der Kuil, W. Altorf-van, Bierman, S. M., de Greeff, S. C., Groenendijk, S. R., Hertroys, R., Kuijper, E. J., Monen, J. C., Notermans, D. W., van den Reek, W. J., Smilde, A. E., Wielders, C. C. H., Zoetigheid, R. E., van den Bijllaardt, W., Kraan, E. M., Mattsson, E. E., da Silva, J. M., de Jong, E., Maraha, B., van Asselt, G. J., Demeulemeester, A., Wintermans, B. B., van Trijp, M., Ott, A., Sinnige, J., Silvis, W., Bakker, L. J., Dorigo-Zetsma, J. W., Waar, K., Bernards, A. T., Hall, M. A. Leversteijn-van, Schaftenaar, E., Nabuurs-Franssen, M. H., Wertheim, H., Bode, L., van Rijn, M., Dinant, S., Pontesilli, O., de Man, P., Wong, M., Muller, A. E., Renders, N. H., Bentvelsen, R. G., Buiting, A. G. M., Vlek, A. L. M., Stam, A. J., Troelstra, A., Overdevest, I. T. M. A., van Meer, M. P. A., dos Santos, C. Oliveira, Wolfhagen, M. J. H. M., Hematology, Internal medicine, AII - Infectious diseases, Medical Microbiology and Infection Prevention, APH - Quality of Care, Elderly care medicine, APH - Aging & Later Life, Graduate School, General Internal Medicine, CCA - Cancer biology and immunology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, CCA - Cancer Treatment and Quality of Life, Clinical Haematology, Experimental Immunology, and APH - Health Behaviors & Chronic Diseases

Subjects
Third-generation-cephalosporin resistance, Colonization, Microbiology (medical), Febrile neutropenia, Bacteremia, General Medicine, Antimicrobial resistance, Empirical antibiotic therapy, Ceftazidime, Infectious Diseases, Carbapenems, Hematologic Neoplasms, Resistance surveillance, Humans, Prospective Studies, Retrospective Studies
Abstract

Objectives Among patients with haematological malignancy, bacteraemia is a common complication during chemotherapy-induced neutropenia. Resistance of gram-negative bacteria (GNB) to third-generation cephalosporins (3GC) is increasing. In order to explore the value of using surveillance cultures to guide empirical treatment e.g. choosing between carbapenem versus ceftazidime- we aimed to assess the distribution of pathogens causing bacteraemia in patients with haematological malignancy, and the proportion of 3GC-resistant GNB (3GC-R GNB) bacteraemia that was preceded by 3GC-R GNB colonization. Methods Using 11 years of data (2008–2018) from the Dutch national antimicrobial resistance surveillance system, we assessed the prevalence of 3GC-R GNB in episodes of bacteraemia, and the proportion of 3GC-R GNB bacteraemia that was preceded by 3GC-R GNB colonization. Colonization was defined as availability of any GNB surveillance isolate in the year before, independent of the causative micro-organism (time-paired isolates). Results We included 3887 patients, representing 4142 episodes of bacteraemia. GNB were identified in 715/4142 (17.3%), of which 221 (30.9%) were 3GC-R GNB. In 139 of these 221 patients a time-paired surveillance culture was available. In 76.2% (106/139) of patients these surveillance cultures already showed 3GC-R GNB isolates in the year prior to the culture date of the 3GC-R GNB positive blood isolate. Conclusions This multi-centre study shows that in patients with haematological malignancy, the majority of 3GC-R GNB bacteraemia is preceded by 3GC-R GNB colonization. Prospective clinical studies are needed to assess the safety and benefits of the use of surveillance-cultures to guide empirical therapy to restrict the empirical use of carbapenems in this population.

Published
2022

3. A genetic cluster of MDR Enterobacter cloacae complex ST78 harbouring a plasmid containing bla VIM-1 and mcr-9 in the Netherlands

Author

Hendrickx, Antoni P. A., Debast, Sylvia, Pérez-Vázquez, María, Schoffelen, Annelot F., Notermans, Daan W., Landman, Fabian, Wielders, Cornelia C. H., Cañada Garcia, Javier E., Flipse, Jacky, de Haan, Angela, Witteveen, Sandra, van Santen-Verheuvel, Marga, de Greeff, Sabine C., Kuijper, Ed, Schouls, Leo M., Maijer-Reuwer, A., Leversteijn-van Hall, M. A., Kluytmans, J. A. J. W., Spijkerman, I. J. B., van Dijk, K., Halaby, T., Zwart, B., Diederen, B. M. W., Voss, A., Dorigo-Zetsma, J. W., Ott, A., Oudbier, J. H., van der Vusse, M., Vlek, A. L. M., Buiting, A. G. M., Bode, L., Paltansing, S., van Griethuysen, A. J., den Reijer, M., van Trijp, M., van Elzakker, E. P. M., Muller, A. E., van der Linden, M. P. M., van Rijn, M., Wolfhagen, M. J. H. M., Waar, K., Kolwijck, E., Silvis, W., Schulin, T., Damen, M., Dinant, S., van Mens, S. P., Melles, D. C., Cohen Stuart, J. W. T., van Ogtrop, M. L., Overdevest, I. T. M. A., van Dam, A. P., Wertheim, H., Frénay, H. M. E., Sinnige, J. C., Mattsson, E. E., Bosboom, R. W., Stam, A., de Jong, E., Roescher, N., Heikens, E., Steingrover, R., Troelstra, A., Bathoorn, E., Trienekens, T. A. M., van Dam, D. W., de Brauwer, E. I. G. B., Stals, F. S., Government of Netherlands, Medical Microbiology and Infection Prevention, AII - Inflammatory diseases, Elderly care medicine, APH - Aging & Later Life, AII - Infectious diseases, Nursing, APH - Health Behaviors & Chronic Diseases, and Experimental Immunology

Subjects
0301 basic medicine, Carbapenem, biology, Brief Report, 030106 microbiology, Broth microdilution, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, Meropenem, Microbiology, 03 medical and health sciences, 030104 developmental biology, Plasmid, AcademicSubjects/MED00290, Colistin, medicine, Multilocus sequence typing, AcademicSubjects/MED00740, AcademicSubjects/MED00230, Enterobacter cloacae, Etest, medicine.drug
Abstract

Background Carbapenemases produced by Enterobacterales are often encoded by genes on transferable plasmids and represent a major healthcare problem, especially if the plasmids contain additional antibiotic resistance genes. As part of Dutch national surveillance, 50 medical microbiological laboratories submit their Enterobacterales isolates suspected of carbapenemase production to the National Institute for Public Health and the Environment for characterization. All isolates for which carbapenemase production is confirmed are subjected to next-generation sequencing. Objectives To study the molecular characteristics of a genetic cluster of Enterobacter cloacae complex isolates collected in Dutch national surveillance in the period 2015–20 in the Netherlands. Methods Short- and long-read genome sequencing was used in combination with MLST and pan-genome MLST (pgMLST) analyses. Automated antimicrobial susceptibility testing (AST), the Etest for meropenem and the broth microdilution test for colistin were performed. The carbapenem inactivation method was used to assess carbapenemase production. Results pgMLST revealed that nine E. cloacae complex isolates from three different hospitals in the Netherlands differed by Conclusions The EclCluster-013 reported here represents an MDR E. cloacae complex ST78 strain containing an IncH12 plasmid carrying both the blaVIM-1 carbapenemase and the mcr-9 colistin resistance gene.

Published
2021

4. Plasmid diversity among genetically related Klebsiella pneumoniae bla KPC-2 and bla KPC-3 isolates collected in the Dutch national surveillance

Author

Hendrickx, APA, Landman, F, de Haan, A, Borst, D, Witteveen, S, van Santen-Verheuvel, MG, van der Heide, HGJ, Schouls, LM, Halaby, T, Steingrover, R, Stuart, JWTC, Melles, DC, Dijk, K, Spijkerman, IJB, Notermans, DW, Oudbier, JH, van Ogtrop, ML, Dam, A, den Reijer, M, Kluytmans, JAJW, van der Linden, MPG, Mattsson, EE, van der Vusse, M, Jong, EM, Maijer-Reuwer, A, van Trijp, M, van Griethuysen, AJ, Ott, A, Bathoorn, E, Sinnige, JC, Heikens, E, de Brauwer, EIGB, Stals, FS, Silvis, W, Dorigo-Zetsma, JW, Waar, K, van Mens, SP, Roescher, N, Voss, A, Wertheim, HFL, Slingerland, BCGC, Frenay, HME, Schulin, T, Diederen, BMW, Bode, Lonneke, Rijn, M, Dinant, S, Damen, M, de Man, P, Leversteijn-van Hall, MA, van Elzakker, EP, Muller, AE, Schneeberger, P, van Dam, DW, Buiting, AG, Vlek, ALM, Stam, A, Troelstra, A, Overdevest, ITMA, Bosboom, RW, Trienekens, TAM, Wolfhagen, MJ, Paltansing, S, Medical Microbiology and Infection Prevention, AII - Infectious diseases, Surgery, Medical Microbiology & Infectious Diseases, Experimental Immunology, Nursing, APH - Aging & Later Life, and APH - Health Behaviors & Chronic Diseases

Subjects
0301 basic medicine, DNA, Bacterial, Klebsiella pneumoniae, 030106 microbiology, lcsh:Medicine, Virulence, Biology, Microbiology, Article, beta-Lactamases, 03 medical and health sciences, Plasmid, polycyclic compounds, Humans, Insertion sequence, lcsh:Science, Pathogen, Netherlands, Genetics, Multidisciplinary, Strain (biology), lcsh:R, High-Throughput Nucleotide Sequencing, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, Resistome, Computational biology and bioinformatics, Klebsiella Infections, 030104 developmental biology, lcsh:Q, Plasmidome, Cation transport
Abstract

Carbapenemase-producingKlebsiella pneumoniaeemerged over the past decades as an important pathogen causing morbidity and mortality in hospitalized patients. For infection prevention and control, it is important to track the spread of bacterial strains in humans including the plasmids they contain. However, little is known concerning the plasmid repertoire amongK. pneumoniaestrains. Therefore, the major aim was to recapitulate the size, contents and diversity of the plasmids of genetically relatedK. pneumoniaestrains harboring the beta-lactamase geneblaKPC-2orblaKPC-3to determine their dissemination in the Netherlands and the former Dutch Caribbean islands from 2014-2019. Next-generation sequencing was combined with long-read third-generation sequencing to reconstruct 18 plasmids ofK. pneumoniae. wgMLST revealed five genetic clusters (termed KpnClusters) comprised ofK. pneumoniae blaKPC-2isolates and four clusters consisted ofblaKPC-3isolates. Each cluster was characterized by a distinct resistome and plasmidome. KpnCluster-019blaKPC-2isolates were found both in the Netherlands and the Caribbean islands.K. pneumoniae blaKPC-3isolates were found in the collection of the Netherlands. The 18 plasmids were mostly unrelated and varied betweenK. pneumoniae blaKPC-2andblaKPC-3clusters. However, the large and medium sized plasmids contained a variety of antibiotic resistance genes, transposons, insertion sequence elements, conjugal transfer systems, cation transport systems, toxin/antitoxin systems, and prophage-related sequence elements. The small plasmids carried genes implicated in virulence. Thus, implementing long-read plasmid sequencing analysis forK. pneumoniaesurveillance provided important insights in the success and understanding of transmission of a KpnCluster-019blaKPC-2strain between the Netherlands and the Caribbean.ImportanceCarbapenemase-producingKlebsiella pneumoniaehas spread globally and is of great concern for debilitated patients.K.pneumoniaeis notorious for spreading antimicrobial resistance genes by plasmids amongEnterobacterales. Combining short and long read sequencing enables reconstruction of plasmids containing antibiotic resistance genes, conjugation machinery, transposons, toxins and/or virulence determinants and thereby enhancing international pathogen surveillance.

Published
2020

5. Plasmid diversity among genetically related Klebsiella pneumoniae bla KPC-2 and bla KPC-3 isolates collected in the Dutch national surveillance

Author

Hendrickx, A.P.A. (Antoni), Landman, F., Haan, A. (Alexander) de, Borst, D., Witteveen, S., Santen-Verheuvel, M. (Marga) van, van der Heide, H.G.J., Schouls, L.M., Halaby, T, Steingrover, R. (Radjin), Stuart, JWTC, Melles, D.C. (Damian), Dijk, K. (Korine) van, Spijkerman, I.J.B., Notermans, D.W. (Daan), Oudbier, J.H., van Ogtrop, M.L., van Dam, A., den Reijer, M., Kluytmans, JAJW, van der Linden, MPG, Mattsson, E.E., van der Vusse, M., Jong, E. (Eefje), Maijer-Reuwer, A., van Trijp, M., van Griethuysen, A.J., Ott, A. (Alewijn), Bathoorn, E., Sinnige, J.C., Heikens, E., Brauwer, E.I.G.B. (E. I G B) de, Stals, F.S. (Frans), Silvis, W., Dorigo-Zetsma, J.W., Waar, K., van Mens, S.P., Roescher, N., Voss, A. (Andreas), Wertheim, H.F.L. (Heiman), Slingerland, B.C.G.C. (Bibi), Frenay, H.M.E., Schülin, T. (Tanja), Diederen, BMW, Bode, L.G.M. (Lonneke), van Rijn, M., Dinant, S., Damen, M. (Mark), de Man, P., Leversteijn-van Hall, M.A., Elzakker, E. van, Muller, A.E., Schneeberger, P., van Dam, D.W., Buiting, AG, Vlek, ALM, Stam, A., Troelstra, A. (Annet), Overdevest, I.T.M.A., Bosboom, R.W., Trienekens, T.A.M., Wolfhagen, M.J., Paltansing, S, Hendrickx, A.P.A. (Antoni), Landman, F., Haan, A. (Alexander) de, Borst, D., Witteveen, S., Santen-Verheuvel, M. (Marga) van, van der Heide, H.G.J., Schouls, L.M., Halaby, T, Steingrover, R. (Radjin), Stuart, JWTC, Melles, D.C. (Damian), Dijk, K. (Korine) van, Spijkerman, I.J.B., Notermans, D.W. (Daan), Oudbier, J.H., van Ogtrop, M.L., van Dam, A., den Reijer, M., Kluytmans, JAJW, van der Linden, MPG, Mattsson, E.E., van der Vusse, M., Jong, E. (Eefje), Maijer-Reuwer, A., van Trijp, M., van Griethuysen, A.J., Ott, A. (Alewijn), Bathoorn, E., Sinnige, J.C., Heikens, E., Brauwer, E.I.G.B. (E. I G B) de, Stals, F.S. (Frans), Silvis, W., Dorigo-Zetsma, J.W., Waar, K., van Mens, S.P., Roescher, N., Voss, A. (Andreas), Wertheim, H.F.L. (Heiman), Slingerland, B.C.G.C. (Bibi), Frenay, H.M.E., Schülin, T. (Tanja), Diederen, BMW, Bode, L.G.M. (Lonneke), van Rijn, M., Dinant, S., Damen, M. (Mark), de Man, P., Leversteijn-van Hall, M.A., Elzakker, E. van, Muller, A.E., Schneeberger, P., van Dam, D.W., Buiting, AG, Vlek, ALM, Stam, A., Troelstra, A. (Annet), Overdevest, I.T.M.A., Bosboom, R.W., Trienekens, T.A.M., Wolfhagen, M.J., and Paltansing, S

Published
2020
Full Text
View/download PDF

6. Molecular characteristics of carbapenemase-producing Enterobacterales in the Netherlands; results of the 2014–2018 national laboratory surveillance

Author

Zwaluw, K. (Kim) van der, Witteveen, S. (S.), Wielders, L. (L.), van Santen, M. (M.), Landman, F. (F.), de Haan, A. (A.), Schouls, L.M., Bosch, T. (T.), Cohen Stuart, J.W.T. (James), Melles, D.C. (Damian), Dijk, K.D. (Karin) van, Visser, C.E., Notermans, D.W. (Daan), Ogtrop, M.L. van, Werdmuller, B.F.M. (B. F.M.), Hees, B.C. (Babette) van, Kluytmans, J.A.J.W. (Jan), van den Bijllaardt, W. (Wouter), Kraan, E.M. (E. M.), van der Linden, M.P.M. (M. P.M.), Mattsson, E.E. (E. E.), Sebens, F. (Fré), de Jong, E. (E.), Frénay, H.M.E. (H. M.E.), Maraha, B. (B.), Griethuysen, A. (Arjanne) van, van Asselt, G.J. (G. J.), Demeulemeester, A. (A.), Wintermans, B.B. (B. B.), Trijp, M.J.C.A. (Marijke) van, Ott, A. (A.), Bathoorn, E. (E.), Lokate, M. (M.), Sinnige, J.C. (J. C.), Brauwer, E.I.G.B. (E. I G B) de, Stals, F.S. (Frans), Silvis, W. (W.), Bakker, L.J. (L. J.), Dorigo-Zetsma, J.W., Ridwan, B. (B.), Waar, K. (K.), Bernards, A.T. (Alexandra), van Mens, S.P. (S. P.), Roescher, N. (N.), Nabuurs-Franssen, M.H. (M. H.), Wertheim, H.F.L. (Heiman), Schülin, T. (Tanja), Diederen, B.M.W. (Bram), Bode, L.G.M. (Lonneke), van Rijn, M. (M.), Dinant, S. (S.), Pontesilli, O. (O.), Man, P. (Peter) de, Leversteijn-van Hall, M.A. (M. A.), Elzakker, E. van, Muller, A.E. (Anouk), Renders, N. (Nicole), van Dam, D.W. (D. W.), Buiting, A.G.M. (Anton), Vlek, A.L.M. (Anne L.M.), Reuland, A. (A.), Frakking, F.N.J. (Florine N.), Overdevest, I. (Ilse), Bosboom, R.W. (R. W.), Trienekens, T.A.M. (T. A.M.), Bruins, M.J. (Marjan), Wolfhagen, M.J.H.M., Zwaluw, K. (Kim) van der, Witteveen, S. (S.), Wielders, L. (L.), van Santen, M. (M.), Landman, F. (F.), de Haan, A. (A.), Schouls, L.M., Bosch, T. (T.), Cohen Stuart, J.W.T. (James), Melles, D.C. (Damian), Dijk, K.D. (Karin) van, Visser, C.E., Notermans, D.W. (Daan), Ogtrop, M.L. van, Werdmuller, B.F.M. (B. F.M.), Hees, B.C. (Babette) van, Kluytmans, J.A.J.W. (Jan), van den Bijllaardt, W. (Wouter), Kraan, E.M. (E. M.), van der Linden, M.P.M. (M. P.M.), Mattsson, E.E. (E. E.), Sebens, F. (Fré), de Jong, E. (E.), Frénay, H.M.E. (H. M.E.), Maraha, B. (B.), Griethuysen, A. (Arjanne) van, van Asselt, G.J. (G. J.), Demeulemeester, A. (A.), Wintermans, B.B. (B. B.), Trijp, M.J.C.A. (Marijke) van, Ott, A. (A.), Bathoorn, E. (E.), Lokate, M. (M.), Sinnige, J.C. (J. C.), Brauwer, E.I.G.B. (E. I G B) de, Stals, F.S. (Frans), Silvis, W. (W.), Bakker, L.J. (L. J.), Dorigo-Zetsma, J.W., Ridwan, B. (B.), Waar, K. (K.), Bernards, A.T. (Alexandra), van Mens, S.P. (S. P.), Roescher, N. (N.), Nabuurs-Franssen, M.H. (M. H.), Wertheim, H.F.L. (Heiman), Schülin, T. (Tanja), Diederen, B.M.W. (Bram), Bode, L.G.M. (Lonneke), van Rijn, M. (M.), Dinant, S. (S.), Pontesilli, O. (O.), Man, P. (Peter) de, Leversteijn-van Hall, M.A. (M. A.), Elzakker, E. van, Muller, A.E. (Anouk), Renders, N. (Nicole), van Dam, D.W. (D. W.), Buiting, A.G.M. (Anton), Vlek, A.L.M. (Anne L.M.), Reuland, A. (A.), Frakking, F.N.J. (Florine N.), Overdevest, I. (Ilse), Bosboom, R.W. (R. W.), Trienekens, T.A.M. (T. A.M.), Bruins, M.J. (Marjan), and Wolfhagen, M.J.H.M.

Abstract

Objectives: Carbapenem resistance mediated by mobile genetic elements has emerged worldwide and has become a major public health threat. To gain insight into the molecular epidemiology of carbapenem resistance in The Netherlands, Dutch medical microbiology laboratories are requested to submit suspected carbapenemase-producing Enterobacterales (CPE) to the National Institute for Public Health and the Environment as part of a national surveillance system. Methods: Meropenem MICs and species identification were confirmed by E-test and MALDI-TOF and carbapenemase production was assessed by the Carbapenem Inactivation Method. Of all submitted CPE, one species/carbapenemase gene combination per person per year was subjected to next-generation sequencing (NGS). Results: In total, 1838 unique isolates were received between 2014 and 2018, of which 892 were unique CPE isolates with NGS data available. The predominant CPE species were Klebsiella pneumoniae (n = 388, 43%), Escherichia coli (n = 264, 30%) and Enterobacter cloacae complex (n = 116, 13%). Various carbapenemase alleles of the same carbapenemase gene resulted in different susceptibilities to meropenem and this effect varied between species. Analyses of NGS data showed variation of prevalence of carbapenemase alleles over time with blaOXA-48 being predominant (38%, 336/892), followed by blaNDM-1 (16%, 145/892). For the first time in the Netherlands, blaOXA-181, blaOXA-232 and blaVIM-4 were detected. The genetic background of K. pneumoniae and E. coli isolates was highly diverse. Conclusions: The CPE population in the Netherlands is diverse, suggesting multiple introductions. The predominant carbapenemase alleles are blaOXA-48 and blaNDM-1. There was a clear association between species, carbapenemase allele and susceptibility to meropenem.

Published
2020
Full Text
View/download PDF

7. Molecular characteristics of carbapenemase-producing Enterobacterales in the Netherlands; results of the 2014–2018 national laboratory surveillance

Author

van der Zwaluw, K., primary, Witteveen, S., additional, Wielders, L., additional, van Santen, M., additional, Landman, F., additional, de Haan, A., additional, Schouls, L.M., additional, Bosch, T., additional, Cohen Stuart, J.W.T., additional, Melles, D.C., additional, van Dijk, K., additional, Visser, C.E., additional, Notermans, D.W., additional, van Ogtrop, M.L., additional, Werdmuller, B.F.M., additional, van Hees, B.C., additional, Kluytmans, J.A.J.W., additional, van den Bijllaardt, W., additional, Kraan, E.M., additional, van der Linden, M.P.M., additional, Mattsson, E.E., additional, Sebens, F.W., additional, de Jong, E., additional, Frénay, H.M.E., additional, Maraha, B., additional, van Griethuysen, A.J., additional, van Asselt, G.J., additional, Demeulemeester, A., additional, Wintermans, B.B., additional, van Trijp, M., additional, Ott, A., additional, Bathoorn, E., additional, Lokate, M., additional, Sinnige, J.C., additional, de Brauwer, E.I.G.B., additional, Stals, F.S., additional, Silvis, W., additional, Bakker, L.J., additional, Dorigo-Zetsma, J.W., additional, Ridwan, B., additional, Waar, K., additional, Bernards, A.T., additional, van Mens, S.P., additional, Roescher, N., additional, Nabuurs-Franssen, M.H., additional, Wertheim, H., additional, Schülin, T., additional, Diederen, B.M.W., additional, Bode, L., additional, van Rijn, M., additional, Dinant, S., additional, Pontesilli, O., additional, de Man, P., additional, Leversteijn-van Hall, M.A., additional, van Elzakker, E.P.M., additional, Muller, A.E., additional, Renders, N.H., additional, van Dam, D.W., additional, Buiting, A.G.M., additional, Vlek, A.L.M., additional, Reuland, A., additional, Frakking, F.N.J., additional, Overdevest, I.T.M.A., additional, Bosboom, R.W., additional, Trienekens, T.A.M., additional, Bruins, M.J., additional, and Wolfhagen, M.J.H.M., additional

Published
2020
Full Text
View/download PDF

8. Third-generation cephalosporin and carbapenem resistance in Streptococcus mitis/oralis. Results from a nationwide registry in the Netherlands

Author

Stuart, J.W.T. Cohen, Weersink, A.J.L., van Dijk, K., Notermans, D., van Ogtrop, M.L., Jager, M.M., Werdmuller, B.F.M., van Hees, B.C., van Keulen, P.H.J., Alblas, J., Altorf-van der Kuil, W., Blijboom, L., de Greeff, S.C., Groenendijk, S., van Heereveld, J., Hertroys, R., Monen, J.C., Notermans, D.W., Reuland, E.A., Schoffelen, A.F., van Triest, M.I., Wielders, C.C.H., Woudt, S.H.S., Kluytmans, J.A.J.W., Kraan, E.M., Mattsson, E.E., Sebens, F.W., de Jong, E., Frénay, H.M.E., Maraha, B., van Griethuysen, A.J., Demeulemeester, A., Wintermans, B.B., van Trijp, M., Ott, A., E. Bathoorn, Lokate, M., Sinnige, J., de Brauwer, E.I.G.B., Stals, F.S., Silvis, W., Bakker, L.J., Dorigo-Zetsma, J.W., Ridwan, B., Waar, K., Bernards, A.T., van Mens, S.P., Roescher, N., Nabuurs-Franssen, M.H., Wertheim, H., Diederen, B.M.W., Bode, L., van Rijn, M., Dinant, S., Pontesilli, O., de Man, P., Hall, M.A. Leversteijn-van, van Elzakker, E.P.M., Muller, A.E., Renders, N.H., van Dam, D.W., Buiting, A.G.M., Vlek, A.L.M., Reuland, A., Frakking, F.N.J., Overdevest, I.T.M.A., Bosboom, R.W., Trienekens, T., Ruijs, G.J.H.M., Wolfhagen, M.J.H.M., and van Prehn, J.

Published
2019
Full Text
View/download PDF

9. Melioidosis in travelers: An analysis of Dutch melioidosis registry data 1985–2018

Author

Birnie, E. (Emma), Savelkoel, J. (Jelmer), Reubsaet, F. (F.), Roelofs, J.J.T.H. (Joris), Soetekouw, R. (Robert), Kolkman, S. (Saskia), Cremers, A.L. (Anne Lia), Grobusch, M.P. (Martin P.), Notermans, D.W. (Daan), Wiersinga, W.J. (W. Joost), Rozemeijer, W. (Wouter), Rijkeboer, A. (Annemieke), Scholing, M. (Maarten), van Dijk, K. (K.), Mascini, E. (Ellen), Van Der Veen, H. (Henk), van den Bijllaardt, W. (Wouter), de Vries, M. (Maaike), Smeets, L.C. (Leonard C.), Ott, A. (Alewijn), van Krimpen, K. (Kees), Herpers, B.L., Watttel-Louis, G.H. (G. Hanke), Waar, K. (Karola), Schwandt, N.L.Q. (Noortje L.Q.), Maat, I. (Ianthe), Dofferhoff, A.S.M. (Anton), Vermeulen, J.N. (Joost N.), Westreenen, M. (Mireille) van, Man, P. (Peter) de, Hofland, R.W. (Regina W.), van Gorp, J. (J.), Thijsen, S.F. (Steven), Velden, L.A. (Lilly Ann) van der, Verduin, C.M. (Cornelis M.), Birnie, E. (Emma), Savelkoel, J. (Jelmer), Reubsaet, F. (F.), Roelofs, J.J.T.H. (Joris), Soetekouw, R. (Robert), Kolkman, S. (Saskia), Cremers, A.L. (Anne Lia), Grobusch, M.P. (Martin P.), Notermans, D.W. (Daan), Wiersinga, W.J. (W. Joost), Rozemeijer, W. (Wouter), Rijkeboer, A. (Annemieke), Scholing, M. (Maarten), van Dijk, K. (K.), Mascini, E. (Ellen), Van Der Veen, H. (Henk), van den Bijllaardt, W. (Wouter), de Vries, M. (Maaike), Smeets, L.C. (Leonard C.), Ott, A. (Alewijn), van Krimpen, K. (Kees), Herpers, B.L., Watttel-Louis, G.H. (G. Hanke), Waar, K. (Karola), Schwandt, N.L.Q. (Noortje L.Q.), Maat, I. (Ianthe), Dofferhoff, A.S.M. (Anton), Vermeulen, J.N. (Joost N.), Westreenen, M. (Mireille) van, Man, P. (Peter) de, Hofland, R.W. (Regina W.), van Gorp, J. (J.), Thijsen, S.F. (Steven), Velden, L.A. (Lilly Ann) van der, and Verduin, C.M. (Cornelis M.)

Abstract

Background: Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is an opportunistic infection across the tropics. Here, we provide a systematic overview of imported human cases in a non-endemic country over a 25-year period. Methods: All 55

Published
2019
Full Text
View/download PDF

10. Melioidosis in travelers: An analysis of Dutch melioidosis registry data 1985-2018

Author

Birnie, E, Savelkoel, J, Reubsaet, F, Roelofs, J, Soetekouw, R, Kolkman, S, Cremers, AL, Grobusch, MP, Notermans, DW, Wiersinga, WJ, Rozemeijer, W, Rijkeboer, A, Scholing, M, Dijk, K, Mascini, EM, de Veen, H, Bijllaardt, Wouter, Boersma - de Vries, M, Smeets, LC, Ott, A, van Krimpen, K, Herpers, BL, Watttel-Louis, GH, Waar, K, Schwandt, NLQ, Maat, I, Dofferhoff, AS, Vermeulen, J N, van Westreenen, Mireille, de Man, P, Hofland, RW, Gorp, J, Thijsen, S, van der Velden, LB, Verduin, CM, Birnie, E, Savelkoel, J, Reubsaet, F, Roelofs, J, Soetekouw, R, Kolkman, S, Cremers, AL, Grobusch, MP, Notermans, DW, Wiersinga, WJ, Rozemeijer, W, Rijkeboer, A, Scholing, M, Dijk, K, Mascini, EM, de Veen, H, Bijllaardt, Wouter, Boersma - de Vries, M, Smeets, LC, Ott, A, van Krimpen, K, Herpers, BL, Watttel-Louis, GH, Waar, K, Schwandt, NLQ, Maat, I, Dofferhoff, AS, Vermeulen, J N, van Westreenen, Mireille, de Man, P, Hofland, RW, Gorp, J, Thijsen, S, van der Velden, LB, and Verduin, CM

Published
2019

11. Effectiviteit en efficiëntie van antibioticaswitch in de ziekenhuisapotheek.

Author

Mitrovic, D., Begari, S., Waar, K., and de Vries, R.

Abstract

OBJECTIVE An earlier switch to oral antibiotics (AB po) can reduce inappropriate use of intravenous antibiotics (AB iv) and comes with certain benefits: decreased workload, reduced risk of complications, and decreased patient discomfort. Therefore, we examined how an earlier switch from AB iv to AB po can be executed optimally in a hospital setting. DESIGN Prospective, explorative intervention study. METHODS Besides the self ­ developed decision support system to detect AB iv, three intervention phases have been set up. Phase 1: pharmacist reports to the microbiologist in the Antimicrobial stewardship team (A ­ Team). Afterwards, the microbiologist discusses the option of switching to AB po with the treating physician. Phase 2: the pharmacist dis ­ cusses the option of switching to AB po with the treating physician. Phase 3: the pharmacist only checks required data for earlier switch to AB po. RESULTS 713 AB iv have been prescribed for 555 patients during the three phases and follow ­ up. For phase 1, 2 and 3 effective-ness was 39%, 48% and 50%, respectively, and pharmacist spent 3.2, 1.4 and 1.0 hours per day, respectively, on AB Switch. Phase 3 has been performed during follow ­ up thus achieving better effectiveness (67%) and better efficiency (0.9 hours per day). CONCLUSION These improvements are due to trainings provided by the A ­ Team, improved documentation of physicians (including indication and antibiotics policy), and increased awareness to switch earlier to AB po. [ABSTRACT FROM AUTHOR]

Published
2018

12. Incidence, clinical implications and impact on public health of infections with Shigella spp. and entero-invasive Escherichia coli (EIEC): results of a multicenter cross-sectional study in the Netherlands during 2016-2017.

Author

van den Beld, Maaike J. C., Warmelink, Esther, Friedrich, Alexander W., Reubsaet, Frans A. G., Schipper, Maarten, de Boer, Richard F., Notermans, Daan W., Petrignani, Mariska W. F., van Zanten, Evert, Rossen, John W. A., Friesema, Ingrid H. M., Kooistra-Smid, A. M. D. (Mirjam), on behalf of the IBESS working group, van Dam, A. P., Svraka-Latifovic, S., Kooistra-Smid, A. M. D., Verweij, J. J., Bruijnesteijn van Coppenraet, L. E. S., Waar, K., and Hermans, M.

Subjects
SHIGELLOSIS, ESCHERICHIA coli, PUBLIC health, FOOD contamination, CROSS-sectional method, SHIGELLA
Abstract

Background: Shigella spp. and entero-invasive E. coli (EIEC) use the same invasive mechanism to cause diarrheal diseases. Public health regulations apply only to Shigella spp. infections, but are hampered by the lack of simple methods to distinguish them from EIEC. In the last decades, molecular methods for detecting Shigella spp. and EIEC were implemented in medical microbiological laboratories (MMLs). However, shigellosis cases identified with molecular techniques alone are not notifiable in most countries. Our study investigates the impact of EIEC versus Shigella spp. infections and molecular diagnosed shigellosis versus culture confirmed shigellosis for re-examination of the rationale for the current public health regulations.Methods: In this multicenter cross-sectional study, fecal samples of patients suspected for gastro-enteritis, referred to 15 MMLs in the Netherlands, were screened by PCR for Shigella spp. or EIEC. Samples were cultured to discriminate between the two pathogens. We compared risk factors, symptoms, severity of disease, secondary infections and socio-economic consequences for (i) culture-confirmed Shigella spp. versus culture-confirmed EIEC cases (ii) culture positive versus PCR positive only shigellosis cases.Results: In 2016-2017, 777 PCR positive fecal samples with patient data were included, 254 of these were culture-confirmed shigellosis cases and 32 were culture-confirmed EIEC cases. EIEC cases were more likely to report ingestion of contaminated food and were less likely to be men who have sex with men (MSM). Both pathogens were shown to cause serious disease although differences in specific symptoms were observed. Culture-negative but PCR positive cases were more likely report travel or ingestion of contaminated food and were less likely to be MSM than culture-positive cases. Culture-negative cases were more likely to suffer from multiple symptoms. No differences in degree of secondary infections were observed between Shigella spp. and EIEC, and culture-negative and culture-positive cases.Conclusions: No convincing evidence was found to support the current guidelines that employs different measures based on species or detection method. Therefore, culture and molecular detection methods for Shigella spp. and EIEC should be considered equivalent for case definition and public health regulations regarding shigellosis. Differences were found regarding risks factors, indicating that different prevention strategies may be required. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

13. Klebsiella pneumoniae species complex: From wastewater to the environment.

Author

Verburg I, Hernández Leal L, Waar K, Rossen JWA, Schmitt H, and García-Cobos S

Abstract

Klebsiella pneumoniae plays a significant role in nosocomial infections and spreading antibiotic resistance, and therefore forms a major threat to public health. In this study, we investigated the role of the wastewater pathway in the spread of pathogenic bacteria and more specifically, in the spread of antibiotic resistant Klebsiella pneumoniae subspecies. Whole-genome sequencing was performed of 185  K. pneumoniae isolates collected from hospital, nursing home, and community wastewater, the receiving wastewater treatment plant (WWTP), and clinical isolates from the investigated hospital. K. pneumoniae isolates from different sources were not genetically related, except for WWTP influent (46.5%) and effluent (62.5%), revealing survival of bacteria from wastewater treatment. The content of antibiotic resistance (ARGs), virulence, and plasmid replicon genes differed between K. pneumoniae subspecies and their origin. While chromosomal bla genes were specific for each K. pneumoniae subspecies, bla genes predicted in plasmid contigs were found in several K. pneumoniae subspecies, implying possible gene transfer between subspecies. Transferable ARGs were most abundant in patients and hospital isolates (70%), but the average number of plasmid replicon genes per isolate was similar across all sources, showing plasmid content being more relevant than plasmid quantity. Most patient (90%) and hospital wastewater (34%) isolates were K. pneumoniae subsp. pneumoniae , and the yersiniabactin cluster genes ybt, fyuA, and irp12 were only found in this subspecies, as were the IncFII(pECLA), IncHI2A, and IncHI2 plasmid replicon genes, suggesting the clinical origin of these type of plasmids., Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results., (© 2024 The Authors. Published by Elsevier B.V.)

Published
2024
Full Text
View/download PDF

14. A prospective multicentre screening study on multidrug-resistant organisms in intensive care units in the Dutch-German cross-border region, 2017 to 2018: the importance of healthcare structures.

Author

Glasner C, Berends MS, Becker K, Esser J, Gieffers J, Jurke A, Kampinga G, Kampmeier S, Klont R, Köck R, von Müller L, Al Naemi N, Ott A, Ruijs G, Saris K, Tami A, Voss A, Waar K, van Zeijl J, and Friedrich AW

Subjects
Aged, Delivery of Health Care, Drug Resistance, Multiple, Bacterial, Female, Humans, Intensive Care Units, Male, Prospective Studies, Cross Infection epidemiology, Cross Infection prevention & control, Methicillin-Resistant Staphylococcus aureus
Abstract

BackgroundAntimicrobial resistance poses a risk for healthcare, both in the community and hospitals. The spread of multidrug-resistant organisms (MDROs) occurs mostly on a local and regional level, following movement of patients, but also occurs across national borders.AimThe aim of this observational study was to determine the prevalence of MDROs in a European cross-border region to understand differences and improve infection prevention based on real-time routine data and workflows.MethodsBetween September 2017 and June 2018, 23 hospitals in the Dutch (NL)-German (DE) cross-border region (BR) participated in the study. During 8 consecutive weeks, patients were screened upon admission to intensive care units (ICUs) for nasal carriage of meticillin-resistant Staphylococcus aureus (MRSA) and rectal carriage of vancomycin-resistant Enterococcus faecium / E. faecalis (VRE), third-generation cephalosporin-resistant Enterobacteriaceae (3GCRE) and carbapenem-resistant Enterobacteriaceae (CRE). All samples were processed in the associated laboratories.ResultsA total of 3,365 patients were screened (median age: 68 years (IQR: 57-77); male/female ratio: 59.7/40.3; NL-BR: n = 1,202; DE-BR: n = 2,163). Median screening compliance was 60.4% (NL-BR: 56.9%; DE-BR: 62.9%). MDRO prevalence was higher in DE-BR than in NL-BR, namely 1.7% vs 0.6% for MRSA (p = 0.006), 2.7% vs 0.1% for VRE (p < 0.001) and 6.6% vs 3.6% for 3GCRE (p < 0.001), whereas CRE prevalence was comparable (0.2% in DE-BR vs 0.0% in NL-BR ICUs).ConclusionsThis first prospective multicentre screening study in a European cross-border region shows high heterogenicity in MDRO carriage prevalence in NL-BR and DE-BR ICUs. This indicates that the prevalence is probably influenced by the different healthcare structures.

Published
2022
Full Text
View/download PDF

15. Effects of Clinical Wastewater on the Bacterial Community Structure from Sewage to the Environment.

Author

Verburg I, van Veelen HPJ, Waar K, Rossen JWA, Friedrich AW, Hernández Leal L, García-Cobos S, and Schmitt H

Abstract

This study pertains to measure differences in bacterial communities along the wastewater pathway, from sewage sources through the environment. Our main focus was on taxa which include pathogenic genera, and genera harboring antibiotic resistance (henceforth referred to as "target taxa"). Our objective was to measure the relative abundance of these taxa in clinical wastewaters compared to non-clinical wastewaters, and to investigate what changes can be detected along the wastewater pathway. The study entailed a monthly sampling campaign along a wastewater pathway, and taxa identification through 16S rRNA amplicon sequencing. Results indicated that clinical and non-clinical wastewaters differed in their overall bacterial composition, but that target taxa were not enriched in clinical wastewater. This suggests that treatment of clinical wastewater before release into the wastewater system would only remove a minor part of the potential total pathogen load in wastewater treatment plants. Additional findings were that the relative abundance of most target taxa was decreased after wastewater treatment, yet all investigated taxa were detected in 68% of the treated effluent samples-meaning that these bacteria are continuously released into the receiving surface water. Temporal variation was only observed for specific taxa in surface water, but not in wastewater samples.

Published
2021
Full Text
View/download PDF

16. Abundance and Antimicrobial Resistance of Three Bacterial Species along a Complete Wastewater Pathway.

Author

Verburg I, García-Cobos S, Hernández Leal L, Waar K, Friedrich AW, and Schmitt H

Abstract

After consumption, antibiotic residues and exposed bacteria end up via the feces in wastewater, and therefore wastewater is believed to play an important role in the spread of antimicrobial resistance (AMR). We investigated the abundance and AMR profiles of three different species over a complete wastewater pathway during a one-year sampling campaign, as well as including antimicrobial consumption and antimicrobial concentrations analysis. A total of 2886 isolates (997 Escherichia coli , 863 Klebsiella spp., and 1026 Aeromonas spp.) were cultured from the 211 samples collected. The bacterial AMR profiles mirrored the antimicrobial consumption in the respective locations, which were highest in the hospital. However, the contribution of hospital wastewater to AMR found in the wastewater treatment plant (WWTP) was below 10% for all antimicrobials tested. We found high concentrations (7-8 logs CFU/L) of the three bacterial species in all wastewaters, and they survived the wastewater treatment (effluent concentrations were around 5 log CFU/L), showing an increase of E. coli in the receiving river after the WWTP discharge. Although the WWTP had no effect on the proportion of AMR, bacterial species and antimicrobial residues were still measured in the effluent, showing the role of wastewater contamination in the environmental surface water.

Published
2019
Full Text
View/download PDF

17. Susceptibility of ESBL Escherichia coli and Klebsiella pneumoniae to fosfomycin in the Netherlands and comparison of several testing methods including Etest, MIC test strip, Vitek2, Phoenix and disc diffusion.

Author

van den Bijllaardt W, Schijffelen MJ, Bosboom RW, Cohen Stuart J, Diederen B, Kampinga G, Le TN, Overdevest I, Stals F, Voorn P, Waar K, Mouton JW, and Muller AE

Subjects
Diagnostic Errors statistics & numerical data, Escherichia coli isolation & purification, Escherichia coli Infections microbiology, Humans, Klebsiella Infections microbiology, Klebsiella pneumoniae isolation & purification, Netherlands, Reproducibility of Results, Anti-Bacterial Agents pharmacology, Escherichia coli drug effects, Fosfomycin pharmacology, Klebsiella pneumoniae drug effects, Microbial Sensitivity Tests methods
Abstract

Objectives: Fosfomycin susceptibility testing is complicated and prone to error. Before using fosfomycin widely in patients with serious infections, acquisition of WT distribution data and reliable susceptibility testing methods are crucial. In this study, the performance of five methods for fosfomycin testing in the routine laboratory against the reference method was evaluated., Methods: Ten laboratories collected up to 100 ESBL-producing isolates each (80 Escherichia coli and 20 Klebsiella pneumoniae). Isolates were tested using Etest, MIC test strip (MTS), Vitek2, Phoenix and disc diffusion. Agar dilution was performed as the reference method in a central laboratory. Epidemiological cut-off values (ECOFFs) were determined for each species and susceptibility and error rates were calculated., Results: In total, 775 E. coli and 201 K. pneumoniae isolates were tested by agar dilution. The ECOFF was 2 mg/L for E. coli and 64 mg/L for K. pneumoniae. Susceptibility rates based on the EUCAST breakpoint of ≤32 mg/L were 95.9% for E. coli and 87.6% for K. pneumoniae. Despite high categorical agreement rates for all methods, notably in E. coli, none of the alternative antimicrobial susceptibility testing methods performed satisfactorily. Due to poor detection of resistant isolates, very high error rates of 23.3% (Etest), 18.5% (MTS), 18.8% (Vitek2), 12.5% (Phoenix) and 12.9% (disc diffusion) for E. coli and 22.7% (Etest and MTS), 16.0% (Vitek2) and 12% (Phoenix) for K. pneumoniae were found. None of the methods adequately differentiated between WT and non-WT populations., Conclusions: Overall, it was concluded that none of the test methods is suitable as an alternative to agar dilution in the routine laboratory.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results