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In recent years, smart city has attracted more and more attention because of its high intelligence and informatization. In the future, smart community, smart parking lot and smart exhibition hall will enter people's life. In these smart services, indoor navigation and positioning technology is the basic technology. High precision indoor and outdoor seamless positioning technology plays a vital role in people's future life, is the science and technology source power to promote public innovation and entrepreneurship, and is an important part of supporting the national strategic needs. At present, indoor navigation is still facing challenges such as high cost, poor adaptability and poor endurance. In this paper, combined with threedimensional( 3D) visualization technology, indoor navigation is designed and applied. The results show that the indoor navigation system combines indoor positioning and artificial intelligence to achieve multichannel positioning and reduce power consumption, which can be widely used in the construction of smart city and bring convenience to people's life.

Purpose: With the spread of multiple drug-resistant bacteria, blaNDM-1 and mcr-9 have been detected in various bacteria worldwide. However, the simultaneous detection of blaNDM-1 and mcr-9 in Enterobacter hormaechei has been rarely reported. This study identified an E. hormaechei strain carrying both blaNDM-1 and mcr-9. We investigated the genetic characteristics of these two resistance genes in detail, elucidating various potential mechanisms by which they may be transmitted. Methods: Bacterial genomic features and possible origins were assessed by whole-genome sequencing (WGS) with Illumina and PacBio platforms and phylogenetic analysis. Subsequent investigations were performed, including antimicrobial susceptibility testing and multilocus sequence typing (MLST). Results: We isolated an E. hormaechei strain DY1901 carrying both blaNDM-1 and mcr-9 from the sputum sample. Susceptibility testing showed that the isolate was multidrug-resistant. Multiple antibiotic resistance genes and virulence genes are widely distributed in DY1901. S1-PFGE, Southern blotting, and plasmid replicon typing showed that DY1901 carried four plasmids. The plasmid carrying mcr-9 was 259Kb in size and belonged to IncHI2, while the plasmid carrying blaNDM-1 was 45Kb in length and belonged to IncX3. Conclusion: The E. hormaechei strain isolated in this study has a broad antibiotic resistance spectrum, posing a challenge to clinical treatment. Plasmids carrying mcr-9 are fusion plasmids, and those taking NDM are widely disseminated in China, suggesting that we should conduct routine genomic surveillance on such plasmids to curb the spread of drug-resistant bacteria in the region. [ABSTRACT FROM AUTHOR]

Emerging evidence has demonstrated the crucial roles of long noncoding RNAs in human cancers, including neuroblastoma (NB). DLX6 antisense RNA 1 (DLX6‐AS1) has been identified as an oncogenic driver in NB. However, the mechanisms of DLX6‐AS1 in NB progression are not fully understood. Our data showed that DLX6‐AS1 was significantly overexpressed in NB tissues and cells. Moreover, DLX6‐AS1 silencing repressed NB cell viability, colony formation, migration, and invasion, and promoted cell cycle arrest and apoptosis in vitro, as well as decreased tumor growth in vivo. Mechanistically, DLX6‐AS1 operated as a miR‐513c‐5p sponge. MiR‐513c‐5p mediated the regulation of DLX6‐AS1 on NB cell malignant progression in vitro. PLK4 was a target of miR‐513c‐5p‐ and DLX6‐AS1‐controlled PLK4 expression via sponging miR‐513c‐5p. Furthermore, the suppressive effect of miR‐513c‐5p overexpression on NB cell malignant progression in vitro was reversed by PLK4 upregulation. Our findings identified a novel regulatory mechanism, the DLX6‐AS1/miR‐513c‐5p/PLK4 axis, in NB progression, highlighting a strong rationale for developing DLX6‐AS1 as a new target for NB management. [ABSTRACT FROM AUTHOR]

Positioning and Navigation (PN) of Martian rovers still faces challenges due to limited observations. In this paper, the PN feasibilities of Mars rovers based on a Gravity-aided Odometry (GO) system are proposed and investigated in terms of numeric simulations and a case study. Statistical features of the Mars gravity field are studied to evaluate the feature diversity of the background map. The Iterative Closest Point (ICP) algorithm is introduced to match gravity measurements with the gravitational map. The trajectories of Mars Exploration Rovers (MER) and Mars Gravity Map 2011 (MGM2011) are used to complete the experiments. Several key factors of GO including odometry errors, measurement uncertainties, and grid resolution of the map are investigated to evaluate their influences on the positioning ability of the system. Simulated experiments indicate that the GO method could provide an alternative positioning solution for Martian surface rovers. [ABSTRACT FROM AUTHOR]

Autonomous navigation with X-ray pulsar can provide continuous and high-precision autonomous navigation information and high-precision time, and standard for the deep space probe and interstellar vehicle. In order to study how to improve the autonomous navigation accuracy and acquire reliable navigation information quickly of the Mars probe, a modified adaptive extended Kalman filtering (AEKF) is studied. Results of the numerical experiment show that the three-dimensional positioning accuracy can achieve 1 000 m in X-axis, 350 m in Y-axis and 350 m in Z-axis. The three-dimensional velocity accuracy is stable within 0. 05 m/s. Moreover, the AEKF can effectively overcome the influence of the initial state deviation and achieve a good estimate value of the state. [ABSTRACT FROM AUTHOR]

Objective: To analyze the clinical manifestation and genetic basis for four children with delayed onset Ornithine transcarbamylase deficiency (OTCD)., Methods: Clinical data of four children with OTCD admitted to the Children's Hospital of the First Affiliated Hospital of Zhengzhou University from January 2020 to April 2021 were reviewed. Peripheral blood samples of the children and their parents were collected and subjected to whole exome sequencing (WES). Bioinformatic analysis and Sanger sequencing verification were carried out to verify the candidate variants. Impact of the candidate variants on the protein structure was also predicted., Results: The clinical manifestations of the four children included vomiting, convulsion and disturbance of consciousness. WES revealed that the child 1 was heterozygous for a c.421C>T (p.R141X) variant in exon 5, children 2 and 3 were hemizygous for a c.119G>A (p.R40H) variant in exon 2, and child 4 was hemizygous for a c.607T>A (p.S203T) variant in exon 5 of the OTC gene. Among these, the c.607T>A variant was unreported previously and predicted to be pathogenic (PM1+PM2_Supporting+PP3+PP4). Bioinformatic analysis has predicted that the variant may result in breakage of hydrogen bonds and alter the protein structure and function. Sanger sequencing confirmed that the variants in children 2 to 4 have derived from their mothers., Conclusion: The pathogenic variants of the OTC gene probably underlay the delayed OTCD in 4 children. The discovery of the c.607T>A variant has enriched the mutational spectrum of the OTC gene.

Purpose: With the spread of multiple drug-resistant bacteria, bla NDM-1 and mcr-9 have been detected in various bacteria worldwide. However, the simultaneous detection of bla NDM-1 and mcr-9 in Enterobacter hormaechei has been rarely reported. This study identified an E. hormaechei strain carrying both bla NDM-1 and mcr-9 . We investigated the genetic characteristics of these two resistance genes in detail, elucidating various potential mechanisms by which they may be transmitted., Methods: Bacterial genomic features and possible origins were assessed by whole-genome sequencing (WGS) with Illumina and PacBio platforms and phylogenetic analysis. Subsequent investigations were performed, including antimicrobial susceptibility testing and multilocus sequence typing (MLST)., Results: We isolated an E. hormaechei strain DY1901 carrying both bla NDM-1 and mcr-9 from the sputum sample. Susceptibility testing showed that the isolate was multidrug-resistant. Multiple antibiotic resistance genes and virulence genes are widely distributed in DY1901. S1-PFGE, Southern blotting, and plasmid replicon typing showed that DY1901 carried four plasmids. The plasmid carrying mcr-9 was 259Kb in size and belonged to IncHI2, while the plasmid carrying bla NDM-1 was 45Kb in length and belonged to IncX3., Conclusion: The E. hormaechei strain isolated in this study has a broad antibiotic resistance spectrum, posing a challenge to clinical treatment. Plasmids carrying mcr-9 are fusion plasmids, and those taking NDM are widely disseminated in China, suggesting that we should conduct routine genomic surveillance on such plasmids to curb the spread of drug-resistant bacteria in the region., Competing Interests: The authors report no conflicts of interest in this work., (© 2022 Liu et al.)