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Altered expression of miR-182 has been observed in various types of human cancer. The purpose of this study was to investigate the expression of miR-182 and its role in prostate cancer (PCa). Expression of miR-182 and ST6GALNAC5 in tumor tissues and the Du145 PCa cell line was analyzed. Cell proliferation assay, colony formation assay, transwell assay, and wound healing assay were performed. The impact of miR-182 on tumor growth was investigated using a xenograft model. The results indicated that expression of miR-182 was higher in PCa tissues and cell lines, while ST6GALNAC5 was decreased. Downregulating miR-182 significantly inhibited the capacities of proliferation and invasion of PC3 and Du145 cells. ST6GALNAC5 was demonstrated to be a target of miR-182 by luciferase assay, and western blot results indicated PI3K/Akt pathway was involved in miR-182 associated effects on PC3 and Du145 cells. The animal experiment suggested that knockdown of miR-182 inhibited tumor growth. Our study proved that miR-182 participated in the proliferation and invasion of PCa cells via mediating expression of ST6GALNAC5 and established a miR-182/ST6GALNAC5/PI3K/AKT axis in regulation of tumor progression. Our investigation provided a basis for further exploration of the application of miR-182 or ST6GALNAC5-associated therapies for PCa patients.

Purpose: To analyze the expression of interleukin-8 in patients with post-renal insufficiency after radiotherapy of abdominal tumors.Methods: 60 patients (experimental group) with post-renal insufficiency and 30 patients (control group) without renal insufficiency after the radiotherapy for abdominal tumors were collected for case-control study. Serum interleukin-8 levels were measured before and after the cystoscopy from September 2017 to March 2019, and the relationship between interleukin-8 levels and serum creatinine, and urea nitrogen, and hydronephrosis was analyzed.Results: The level of serum interleukin-8 levels were significantly increased in patients with post-renal insufficiency after radiotherapy for abdominal tumors(P

Sen Wang, Wei-Cheng Gao, San-San Chen, Liang Bai, Li Luo, Xiang-Guang Zheng, You Luo Department of Urology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China Objective: To define the survival effect of surgery of primary adrenal malignant lesions in metastatic adrenocortical carcinoma (ACC) patients. Patients and methods: We used the Surveillance, Epidemiology and End Results (SEER) database (1973–2014) to identify metastatic ACC patients (stage IV by using European Network for the Study of Adrenal Tumors stage classification). Correlated variables, including age, sex, race, tumor laterality, treatment modality, lymph node dissection, surgery of metastatic site, tumor size, and tumor stage, were extracted. Univariate and multivariate Cox regression analyses were used to define the efficacy of surgery on survival outcomes, including overall survival and cancer-specific survival of ACC. Results: There were 290 metastatic ACC patients identified from the database. The overall median survival time was 7 (95% CI, 6–8) months. Among these patients, 118 patients received primary site surgery and 172 patients did not. In both univariate and multivariate analyses, primary site surgery significantly improved both overall (hazard ratio 0.413, 95% CI, 0.299–0.571, P

The occurrence of the mode localization is studied in a structure of a single-layer spherical reticulated shell. The matrix perturbation method is used to analyze the mechanism of the occurrence of mode localization. The dynamical characteristics of this structure, especially the effects of the variation of the stiffness parameter on the vibration modes of the structure, were analyzed by numerical simulations. An amplitude significance coefficient is proposed as a measure of the degree of the mode localization. The results show that small deviations of the stiffness parameter can cause significant changes to the structural dynamics characteristics; the mode localization does occur for some particular modes of this structure in the case of random stiffness mistuning. Preliminary analysis found that the mode can be considered as strongly localized when the amplitude significance coefficient is greater than 8.