257 results on '"Wisplinghoff, Hilmar"'
Search Results
2. Semitendinosus tendons are commonly contaminated with skin flora during graft harvest for anterior cruciate ligament reconstruction
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Offerhaus, Christoph, Jaecker, Vera, Shafizadeh, Sven, Müller, Leonard, Hahne, Heiko, Wisplinghoff, Hilmar, and Jazmati, Nathalie
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- 2023
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3. The tongue microbiome of young patients with chronic kidney disease and their healthy mothers
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Hoefer, Karolin C., Weber, Lutz T., Barbe, Anna Greta, Graf, Isabelle, Thom, Stefanie, Nowag, Angela, Scholz, Claus J., Wisplinghoff, Hilmar, Noack, Michael J., and Jazmati, Nathalie
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- 2024
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4. Evidence of Bacterial Metabolism in Synovial Fluid of Patients With Graft Failure After Anterior Cruciate Ligament Reconstruction: A Microbiological Comparison of Primary Anterior Cruciate Ligament and Hamstring Tendon Autograft Ruptures
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Offerhaus, Christoph, Leutheuser, Sebastian, Jaecker, Vera, Shafizadeh, Sven, Bardtke, Lena, Wisplinghoff, Hilmar, and Jazmati, Nathalie
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- 2024
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5. Combined analysis of gut microbiota, diet and PNPLA3 polymorphism in biopsy‐proven non‐alcoholic fatty liver disease
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Lang, Sonja, Martin, Anna, Zhang, Xinlian, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria JGT, Krawczyk, Marcin, Nowag, Angela, Kretzschmar, Anne, Scholz, Claus, Kasper, Philipp, Roderburg, Christoph, Mohr, Raphael, Lammert, Frank, Tacke, Frank, Schnabl, Bernd, Goeser, Tobias, Steffen, Hans‐Michael, and Demir, Münevver
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Biomedical and Clinical Sciences ,Clinical Sciences ,Chronic Liver Disease and Cirrhosis ,Liver Disease ,Nutrition ,Digestive Diseases ,Prevention ,Genetics ,Aetiology ,2.1 Biological and endogenous factors ,Metabolic and endocrine ,Inflammatory and immune system ,Oral and gastrointestinal ,Good Health and Well Being ,Aged ,Biopsy ,Cross-Sectional Studies ,Diet ,Gastrointestinal Microbiome ,Humans ,Lipase ,Liver ,Membrane Proteins ,Non-alcoholic Fatty Liver Disease ,Polymorphism ,Single Nucleotide ,microbiome ,microbiota ,NAFLD ,NASH ,nutrition ,PNPLA3 ,PNPLA3 ,Gastroenterology & Hepatology ,Clinical sciences - Abstract
Background and aimsNon-alcoholic fatty liver disease (NAFLD) is a global health burden. Risk factors for disease severity include older age, increased body mass index (BMI), diabetes, genetic variants, dietary factors and gut microbiota alterations. However, the interdependence of these factors and their individual impact on disease severity remain unknown.MethodsIn this cross-sectional study, we performed 16S gene sequencing using fecal samples, collected dietary intake, PNPLA3 gene variants and clinical and liver histology parameters in a well-described cohort of 180 NAFLD patients. Principal component analyses were used for dimensionality reduction of dietary and microbiota data. Simple and multiple stepwise ordinal regression analyses were performed.ResultsComplete data were available for 57 NAFLD patients. In the simple regression analysis, features associated with the metabolic syndrome had the highest importance regarding liver disease severity. In the multiple regression analysis, BMI was the most important factor associated with the fibrosis stage (OR per kg/m2 : 1.23, 95% CI 1.10-1.37, P
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- 2021
6. Intestinal Virome Signature Associated With Severity of Nonalcoholic Fatty Liver Disease
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Lang, Sonja, Demir, Münevver, Martin, Anna, Jiang, Lu, Zhang, Xinlian, Duan, Yi, Gao, Bei, Wisplinghoff, Hilmar, Kasper, Philipp, Roderburg, Christoph, Tacke, Frank, Steffen, Hans-Michael, Goeser, Tobias, Abraldes, Juan G, Tu, Xin M, Loomba, Rohit, Stärkel, Peter, Pride, David, Fouts, Derrick E, and Schnabl, Bernd
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Biomedical and Clinical Sciences ,Clinical Sciences ,Nutrition and Dietetics ,Chronic Liver Disease and Cirrhosis ,Digestive Diseases ,Clinical Research ,Liver Disease ,Oral and gastrointestinal ,Infection ,Adult ,Aged ,Case-Control Studies ,Cross-Sectional Studies ,Feces ,Female ,Gastrointestinal Microbiome ,Humans ,Intestines ,Liver Cirrhosis ,Male ,Middle Aged ,Non-alcoholic Fatty Liver Disease ,Prospective Studies ,Severity of Illness Index ,Virome ,Young Adult ,Microbiota ,Biomarker ,Prognostic Factor ,Progression ,Neurosciences ,Paediatrics and Reproductive Medicine ,Gastroenterology & Hepatology ,Clinical sciences ,Nutrition and dietetics - Abstract
Background & aimsAlterations in the gut microbiome have been associated with the severity of nonalcoholic fatty liver disease (NAFLD). Previous studies focused exclusively on the bacteria in the microbiome; we investigated changes in the viral microbiome (virome) in patients with NAFLD.MethodsIn a prospective, cross-sectional, observational study, we extracted RNA and DNA virus-like particles from fecal samples from 73 patients with NAFLD: 29 patients had an NAFLD Activity Score (NAS) of 0-4, 44 patients had an NAS of 5-8 or liver cirrhosis (LCI), 37 patients had F0-F1 fibrosis, and 36 patients had F2-F4 fibrosis. As controls, 9 individuals without liver disease and 13 patients with mild primary biliary cholangitis were included in the analysis. We performed shotgun metagenomic sequencing of virus-like particles.ResultsPatients with NAFLD and NAS 5-8/LCI had a significant decrease in intestinal viral diversity compared with patients with NAFLD and NAS 0-4 or control individuals. The presence of more advanced NAFLD was associated with a significant reduction in the proportion of bacteriophages compared with other intestinal viruses. Using multivariate logistic regression analysis with leave-1-out cross validation, we developed a model, including a viral diversity index and simple clinical variables, that identified patients with NAS 5-8/LCI with an area under the curve of 0.95 (95% confidence interval, 0.91-0.99) and F2-F4 fibrosis with an area under the curve of 0.88 (95% confidence interval, 0.80-0.95). Addition of data on viral diversity significantly improved multivariate models, including those based on only clinical parameters or bacterial diversity.ConclusionsIn a study of fecal viromes from patients with NAFLD and control individuals, we associated histologic markers of NAFLD severity with significant decreases in viral diversity and proportion of bacteriophages. We developed a model based on fecal viral diversity and clinical data that identifies patients with severe NAFLD and fibrosis more accurately than models based only on clinical or bacterial data.
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- 2020
7. Organmykosen
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Cornely, Oliver A., primary and Wisplinghoff, Hilmar, additional
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- 2023
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8. Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children
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Dewald, Felix, Suárez, Isabelle, Johnen, Ronja, Grossbach, Jan, Moran-Tovar, Roberto, Steger, Gertrud, Joachim, Alexander, Rubio, Gibran Horemheb, Fries, Mira, Behr, Florian, Kley, Joao, Lingnau, Andreas, Kretschmer, Alina, Gude, Carina, Baeza-Flores, Guadelupe, del Valle, David Laveaga, Roblero-Hernandez, Alberto, Magana-Cerino, Jesus, Hernandez, Adriana Torres, Ruiz-Quinones, Jesus, Schega, Konstantin, Linne, Viktoria, Junker, Lena, Wunsch, Marie, Heger, Eva, Knops, Elena, Di Cristanziano, Veronica, Meyer, Meike, Hünseler, Christoph, Weber, Lutz T., Lüers, Jan-Christoffer, Quade, Gustav, Wisplinghoff, Hilmar, Tiemann, Carsten, Zotz, Rainer, Jomaa, Hassan, Pranada, Arthur, Herzum, Ileana, Cullen, Paul, Schmitz, Franz-Josef, Philipsen, Paul, Kirchner, Georg, Knabbe, Cornelius, Hellmich, Martin, Buess, Michael, Wolff, Anna, Kossow, Annelene, Niessen, Johannes, Jeworutzki, Sebastian, Schräpler, Jörg-Peter, Lässig, Michael, Dötsch, Jörg, Fätkenheuer, Gerd, Kaiser, Rolf, Beyer, Andreas, Rybniker, Jan, and Klein, Florian
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- 2022
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9. Intensive oral prophylaxis does not alter the tongue microbiome in young patients with chronic kidney disease: longitudinal, randomized, controlled study.
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Hoefer, Karolin C., Weber, Lutz T., Barbe, Anna Greta, Graf, Isabelle, Thom, Stefanie, Ehren, Rasmus, Nowag, Angela, Wisplinghoff, Hilmar, Noack, Michael J., Scholz, Claus J., and Jazmati, Nathalie
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DENTAL prophylaxis ,CHRONIC kidney failure ,FALSE discovery rate ,PEDIATRIC nephrology ,HEALTH insurance - Abstract
Introduction: Gingivitis is a common intraoral disease in patients with chronic kidney disease (CKD), which poses a particular interdisciplinary challenge. We aimed to determine the influence of an intensive oral prophylaxis program (OPP) compared to standard prevention measures on the tongue microbiome of young patients with CKD. Methods: Thirty patients with CKD (mean age 14.2 ± 5.2 years) and generalized gingivitis were included. The effects of the intensive OPP were compared with standard prophylaxis according to statutory health insurance (treatment as usual, TAU) as a control. Tongue swabs were taken from the patients at baseline (t1) and after 3 (t2) and 6 (t3) months. Next-generation sequencing of 16S rDNA genes was used to quantitatively characterize microbial communities. Results: There were no differences in the abundance, richness, or diversity of the observed genera and species between the two study groups at baseline or after 3 or 6 months. Furthermore, no change in predefined gingivitis and oral health bacterial clusters were found. At the phylum level, Firmicutes were decreased after intervention in the TAU group (t2
TAU 42.9 ± 7.1 to t3TAU 34.8 ± 4.7 (npairs =14), p=0.003; false discovery rate 0.02). The decrease of Firmicutes was not significant in the OPP group. Conclusions: Despite the intensity of dental prophylaxis and decreasing clinical signs of inflammation and decreasing plaque amount, no clinically relevant changes in the tongue microbiome were observed. Our results confirm the conserved and stable nature of the tongue microbiome, even in children with CKD. [ABSTRACT FROM AUTHOR]- Published
- 2024
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10. Rapid high-throughput processing of tissue samples for microbiological diagnosis of periprosthetic joint infections using bead-beating homogenization
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Jazmati, Nathalie, primary, Liebold, Carolin, additional, Offerhaus, Christoph, additional, Volkenand, Annika, additional, Grote, Stefan, additional, Pöpsel, Juliane, additional, Körber-Irrgang, Barbara, additional, Hoppe, Thomas, additional, and Wisplinghoff, Hilmar, additional
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- 2024
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11. Antigen specificity and cross-reactivity drive functionally diverse anti-Aspergillus fumigatus T cell responses in cystic fibrosis
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Schwarz, Carsten, Eschenhagen, Patience, Schmid, Henrijette, Hohnstein, Thordis, Grehn, Claudia, Iwert, Christina, Roehmel, Jobst, Steinke, Eva, Stahl, Mirjam, Lozza, Laura, Tikhonova, Ekaterina, Rosati, Elisa, Stervbo, Ulrik, Babel, Nina, Mainz, Jochen G., Wisplinghoff, Hilmar, Ebel, Frank, Jia, Lei-Jie, Blango, Matthew G., Hortschansky, Peter, Brunke, Sascha, Hube, Bernhard, Brakhage, Axel A., Kniemeyer, Olaf, Scheffold, Alexander, and Bacher, Petra
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Cross reactions (Immunology) -- Health aspects ,Cystic fibrosis -- Physiological aspects ,Immune response -- Health aspects ,CD4 lymphocytes -- Physiological aspects -- Health aspects ,Aspergillosis -- Physiological aspects -- Risk factors ,Health care industry - Abstract
BACKGROUND. The fungus Aspergillus fumigatus causes a variety of clinical phenotypes in patients with cystic fibrosis (pwCF). Th cells orchestrate immune responses against fungi, but the types of A. fumigatus-specific Th cells in pwCF and their contribution to protective immunity or inflammation remain poorly characterized. METHODS. We used antigen-reactive T cell enrichment (ARTE) to investigate fungus-reactive Th cells in peripheral blood of pwCF and healthy controls. RESULTS. We show that clonally expanded, high-avidity A. fumigatus-specific effector Th cells, which were absent in healthy donors, developed in pwCF. Individual patients were characterized by distinct Th1-, Th2-, or Th17- dominated responses that remained stable over several years. These different Th subsets target different A. fumigatus proteins, indicating that differential antigen uptake and presentation directs Th cell subset development. Patients with allergic bronchopulmonary aspergillosis (ABPA) are characterized by high frequencies of Th2 cells that cross- recognize various filamentous fungi. CONCLUSION. Our data highlight the development of heterogenous Th responses targeting different protein fractions of a single fungal pathogen and identify the development of multispecies cross- reactive Th2 cells as a potential risk factor for ABPA. FUNDING. German Research Foundation (DFG), under Germany's Excellence Strategy (EXC 2167-390884018 'Precision Medicine in Chronic Inflammation' and EXC 2051-390713860 'Balance of the Microverse'); Oskar Helene Heim Stiftung; Christiane Herzog Stiftung; Mukoviszidose Institut gGmb; German Cystic Fibrosis Association Mukoviszidose e.V; German Federal Ministry of Education and Science (BMBF) InfectControl 2020 Projects AnDiPath (BMBF 03ZZ0838A+B)., Introduction Humans are constantly exposed to ubiquitous airborne fungi such as Aspergillus fumigatus. Despite this continuous challenge, healthy individuals rarely develop Aspergillus-associated diseases. This is likely due to rapid clearing [...]
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- 2023
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12. Invasive Scedosporium spp. and Lomentospora prolificans infections in pediatric patients: Analysis of 55 cases from FungiScope® and the literature
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Seidel, Danila, Hassler, Angela, Salmanton-García, Jon, Koehler, Philipp, Mellinghoff, Sibylle C., Carlesse, Fabianne, Cheng, Matthew P., Falces-Romero, Iker, Herbrecht, Raoul, Jover Sáenz, Alfredo, Klimko, Nikolai, Mareş, Mihai, Lass-Flörl, Cornelia, Soler-Palacín, Pere, Wisplinghoff, Hilmar, Cornely, Oliver A., Pana, Zoi, and Lehrnbecher, Thomas
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- 2020
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13. Organmykosen
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Cornely, Oliver, primary and Wisplinghoff, Hilmar, additional
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- 2021
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14. Fecal microbiota transplantation in a kidney transplant recipient with recurrent urinary tract infection
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Biehl, Lena M., Cruz Aguilar, Rebeca, Farowski, Fedja, Hahn, Werner, Nowag, Angela, Wisplinghoff, Hilmar, and Vehreschild, Maria J. G. T.
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- 2018
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15. Antimicrobial resistance of clinical Enterobacterales isolates from urine samples, Germany, 2016 to 2021
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Stoltidis-Claus, Carolin, primary, Rosenberger, Kerstin Daniela, additional, Mandraka, Falitsa, additional, Quante, Xenia, additional, Gielen, Jörg, additional, Hoffmann, Dennis, additional, Wisplinghoff, Hilmar, additional, and Jazmati, Nathalie, additional
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- 2023
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16. Prediction of advanced fibrosis in non-alcoholic fatty liver disease using gut microbiota-based approaches compared with simple non-invasive tools
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Lang, Sonja, Farowski, Fedja, Martin, Anna, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Krawczyk, Marcin, Nowag, Angela, Kretzschmar, Anne, Scholz, Claus, Kasper, Philipp, Roderburg, Christoph, Lammert, Frank, Goeser, Tobias, Steffen, Hans-Michael, and Demir, Münevver
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- 2020
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17. Analyzing candidemia guideline adherence identifies opportunities for antifungal stewardship
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Mellinghoff, Sibylle C., Hartmann, Pia, Cornely, Florian B., Knauth, Laura, Köhler, Felix, Köhler, Philipp, Krause, Carolin, Kronenberg, Christine, Kranz, Sarah-Leonie, Menon, Vidya, Müller, Hannah, Naendrup, Jan-Hendrik, Pützfeld, Stefan, Ronge, Anna, Rutz, Jule, Seidel, Danila, Wisplinghoff, Hilmar, and Cornely, Oliver A.
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- 2018
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18. Prevalence and distribution of Legionella spp in potable water systems in Germany, risk factors associated with contamination, and effectiveness of thermal disinfection
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Kruse, Eva-Brigitta, Wehner, Arno, and Wisplinghoff, Hilmar
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- 2016
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19. Dietary omega-6/omega-3 ratio is not associated with gut microbiota composition and disease severity in patients with nonalcoholic fatty liver disease
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Heinzer, Kathrin, primary, Lang, Sonja, additional, Farowski, Fedja, additional, Wisplinghoff, Hilmar, additional, Vehreschild, Maria J.G.T., additional, Martin, Anna, additional, Nowag, Angela, additional, Kretzschmar, Anne, additional, Scholz, Claus Jürgen, additional, Roderburg, Christoph, additional, Mohr, Raphael, additional, Tacke, Frank, additional, Kasper, Philipp, additional, Goeser, Tobias, additional, Steffen, Hans-Michael, additional, and Demir, Münevver, additional
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- 2022
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20. Efficacy of two antiseptic regimens on skin colonization of insertion sites for two different catheter types: a randomized, clinical trial
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Lutz, Juergen Thomas, Diener, Isabel Victoria, Freiberg, Kerstin, Zillmann, Robert, Shah-Hosseini, Kija, Seifert, Harald, Berger-Schreck, Bettina, and Wisplinghoff, Hilmar
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- 2016
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21. Invasive Trichoderma spp. infections: clinical presentation and outcome of cases from the literature and the FungiScope® registry
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Sal, Ertan, primary, Stemler, Jannik, additional, Salmanton-García, Jon, additional, Falces-Romero, Iker, additional, Kredics, László, additional, Meyer, Elisabeth, additional, Würstl, Benjamin, additional, Lass-Flörl, Cornelia, additional, Racil, Zdenek, additional, Klimko, Nikolay, additional, Cesaro, Simone, additional, Kindo, Anupma Jyoti, additional, Wisplinghoff, Hilmar, additional, Koehler, Philipp, additional, Cornely, Oliver A, additional, and Seidel, Danila, additional
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- 2022
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22. Candida‐reactive T cells for the diagnosis of invasive Candida infection of the lumbar vertebral spine
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Koehler, Felix C., Cornely, Oliver A., Wisplinghoff, Hilmar, Chang, De‐Hua, Richter, Anne, and Koehler, Philipp
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- 2018
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23. Pseudomonas spp., Acinetobacter spp. and Miscellaneous Gram-Negative Bacilli
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Wisplinghoff, Hilmar, primary
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- 2017
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24. List of Contributors
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Abrahamian, Fredrick M., primary, Aldape, Michael J., additional, Aldasoro, Edelweiss, additional, Allen, Upton D., additional, Al-Sum, Hythem, additional, Anadkat, Milan J., additional, Anders, Katherine, additional, Angelakis, Emmanouil, additional, Angus, Brian John, additional, Antoniadou, Anastasia, additional, Arena, Fabio, additional, Arends, Joop E., additional, Arribas, Jose R., additional, Artenstein, Andrew W., additional, Atherton, John C., additional, Aucott, John N., additional, Aw, Tar-Ching, additional, Babcock, Hilary M., additional, Bailey, Robin, additional, Bailey, Thomas C., additional, Banks, Adam Z., additional, Barillo, David J., additional, Barrette, Ernie-Paul, additional, Bauer, Martijn P., additional, Bayston, Roger, additional, Beard, C. Ben, additional, Beardsley, Justin, additional, Beeching, Nick J., additional, Bégué, Rodolfo E., additional, Beldi, Guido, additional, Benson, Constance A., additional, Berbari, Elie F., additional, Berenger, Jean-Michel, additional, Berger, Christoph, additional, Bernardino, Jose I., additional, Bille, Jacques, additional, Billioux, Alexander C., additional, Bitnun, Ari, additional, Blair, Iain, additional, Blanche, Stéphane, additional, Bleck, Thomas P., additional, Bleeker-Rovers, Chantal P., additional, Bleijenberg, Gijs, additional, Bloch, Karen C., additional, Blum, Johannes, additional, Blumberg, Emily A., additional, Bonomo, Robert A., additional, Bonten, Marc J.M., additional, Bourayou, Rafik, additional, Bouza, Emilio, additional, Brandt, K. Ashley, additional, Bretelle, Florence, additional, Brisse, Sylvain, additional, Britton, Warwick J., additional, Brook, Itzhak, additional, Brouwer, Matthijs C., additional, Browne, Sarah K., additional, Bryant, Amy E., additional, Bühler, Silja, additional, Bulger, Eileen M., additional, Buller, R. Mark L., additional, Burke, Leah A., additional, Burri, Christian, additional, Butler, Marcus W., additional, Calandra, Thierry, additional, Calfee, David P., additional, Calvo-Cano, Antonia, additional, Cameron, D. William, additional, Carcillo, Joseph A., additional, Carson, Gail, additional, Chambers, Stephen T., additional, Charrel, Remi N., additional, Nguyen, Vinh Chau Van, additional, Chevaliez, Stéphane, additional, Chiller, Tom M., additional, Christaki, Eirini, additional, Chung, Kevin K., additional, Clifford, David B., additional, Clumeck, Nathan, additional, Cohen, Jonathan, additional, Collinge, John, additional, Conlon, Christopher P., additional, Conrad, Curdin, additional, Cooke, Fiona J., additional, Cope, Jennifer Rittenhouse, additional, Corey, G. Ralph, additional, Cross, John H., additional, Cunha, Burke A., additional, Cunha, Cheston B., additional, D'Journo, Benoit, additional, Daikos, George L., additional, Daniels, Johannes M.A., additional, Davidson, Robert N., additional, Day, Nicholas P.J., additional, De Cock, Kevin M., additional, de Silva, Thushan I., additional, de Vries, Henry J.C., additional, de Wit, Stéphane, additional, Delaloye, Julie, additional, Denning, David W., additional, Dennis, David T., additional, Dhanireddy, Shireesha, additional, Dielubanza, Elodi J., additional, Diemert, David J., additional, Doganay, Mehmet, additional, Doherty, Tom, additional, Dolecek, Christiane, additional, Dondorp, Arjen M., additional, Douglas, Abby, additional, Drancourt, Michel, additional, Dubourg, Grégory, additional, Dudley, Michael N., additional, Durand, Guillaume, additional, Eckhardt, Benjamin J., additional, Efstratiou, Androulla, additional, Ekkelenkamp, Miquel B., additional, Eranki, Ambika, additional, Erdem, Hakan, additional, Escota, Gerome V., additional, Evans, Heather L., additional, Eziefula, Alice Chijioke, additional, Fenollar, Florence, additional, Fenwick, Alan, additional, Fierer, Joshua, additional, Finch, Roger G., additional, Fleckenstein, James M., additional, Forstner, Christina, additional, Foschi, Federico, additional, Fournier, Pierre-Edouard, additional, French, Martyn A., additional, Gage, Kenneth L., additional, Garcia, Lynne S., additional, Gascon, Joaquim, additional, Gastañaduy, Arturo S., additional, Gautret, Philippe, additional, Geisler, William M., additional, Ghanem, Khalil G., additional, Giani, Tommaso, additional, Giannella, Maddalena, additional, Gilliam, Bruce L., additional, Gilliet, Michel, additional, Glaser, Carol A., additional, Glupczynski, Youri, additional, Gnann, John W., additional, Goldstein, Ellie J.C., additional, Gottstein, Bruno, additional, Gouriet, Frederique, additional, Gravitt, Patti E., additional, Green, Michael D., additional, Green, Stephen T., additional, Groll, Andreas H., additional, Gulick, Roy M., additional, Gupta, Arjun, additional, Habib, Gilbert, additional, Harbarth, Stephan, additional, Harris, Marianne, additional, Hayden, Frederick G., additional, Hetem, David J., additional, Hill, Philip C., additional, Hirschel, Bernard, additional, Hodowanec, Aimee C., additional, Hoffart, Louis, additional, Hoffmann, Christian, additional, Holland, Steven M., additional, Horby, Peter W., additional, Horne, David J., additional, Hraiech, Sami, additional, Hull, Mark W., additional, Huttner, Angela, additional, Ingram, Richard J.M., additional, Islam, Jasmin, additional, Ison, Michael G., additional, James, Scott H., additional, Jenkins, Claire, additional, Jenkins, Stephen G., additional, Jensen, Jørgen Skov, additional, Johnston, Christine, additional, Jones, Theodore B., additional, Jordan, Stephen J., additional, Julian, Kathleen G., additional, Kato, Yasuyuki, additional, Kauffman, Carol A., additional, Kaye, Keith S., additional, Keane, Michael P., additional, Keeney, James, additional, Kelly, Paul, additional, Kent, Stephen J., additional, Kern, Winfried V., additional, Keynan, Yoav, additional, Kim, Andrea A., additional, Koné-Paut, Isabelle, additional, Kosmidis, Chris, additional, Kroes, Aloys C.M., additional, Kroon, Frank P., additional, Ksiazek, Thomas G., additional, Kuhlmann, F. Matthew, additional, Kuijper, Ed J., additional, Kwon, Jennie H., additional, Kyei, George B., additional, Lacombe, Karine, additional, Lagacé-Wiens, Philippe, additional, Lagier, Jean-Christophe, additional, Lamagni, Theresa, additional, Landraud, Luce, additional, Lanternier, Fanny, additional, LaPlante, Kerry L., additional, Lawn, Stephen D., additional, Lawrence, Steven J., additional, Leblebicioglu, Hakan, additional, Lee, Nelson, additional, Leggett, James E., additional, Lehours, Philippe, additional, Levy, Pierre-Yves, additional, Leyh, Rainer G., additional, Lillis, Rebecca A., additional, Limmathurotsakul, Direk, additional, Lin, Jennifer, additional, Lindquist, H.D. Alan, additional, Lipsky, Benjamin A., additional, Liscynesky, Christina, additional, Looney, David, additional, Lortholary, Olivier, additional, Lowy, Franklin D., additional, Luft, Benjamin J., additional, Mackowiak, Philip A., additional, MacPherson, Paul A., additional, Maghraoui-Slim, Valérie, additional, Mallon, Patrick W., additional, Mangino, Julie E., additional, Manuel, Oriol, additional, Marchetti, Oscar, additional, Marks, Kristen M., additional, Marr, Kieren A., additional, Marrazzo, Jeanne, additional, Marschall, Jonas, additional, Martin, David H., additional, Matonti, Frédéric, additional, Matulewicz, Richard S., additional, Mayer, Kenneth H., additional, McCulloh, Russell J., additional, McGready, Rose, additional, Mdodo, Rennatus, additional, Mead, Simon, additional, Mégraud, Francis, additional, Meintjes, Graeme, additional, Metcalf, Sarah C., additional, Michaels, Marian G., additional, Migliori, Giovanni Battista, additional, Miles, Michael A., additional, Miller, Alastair, additional, Mimiaga, Matthew J., additional, Mingeot-Leclercq, Marie-Paule, additional, Misch, Elizabeth Ann, additional, Mitreva, Makedonka, additional, Montaner, Julio S.G., additional, Moore, Caroline B., additional, Muñoz, Patricia, additional, Muñoz, Jose, additional, Murray, Clinton K., additional, Musso, Didier, additional, Mutengo, Mable, additional, Mutizwa, Misha M., additional, Naber, Kurt G., additional, Natarajan, Pavithra, additional, Neme, Santiago, additional, Newton, Paul N., additional, Nichols, Ronald A., additional, Nicolle, Lindsay E., additional, Nosten, François, additional, Notarangelo, Luigi D., additional, Nutman, Thomas B., additional, Nyirjesy, Paul, additional, O'Connell, P. Ronan, additional, Opal, Steven M., additional, Ormerod, L. Peter, additional, Osmon, Douglas R., additional, Pankert, Marie Boulze, additional, Pantaleo, Giuseppe, additional, Papazian, Laurent, additional, Parente, Diane M., additional, Parola, Philippe, additional, Parsaei, Shadi, additional, Pascual, Manuel A., additional, Patel, Rupa, additional, Patrozou, Eleni, additional, Pawlotsky, Jean-Michel, additional, Peacock, Sharon J., additional, Pechère, Jean-Claude, additional, Pelegrin, Ivan, additional, Peters, Barry S., additional, Peters, Edgar J.G., additional, Petersen, Jeannine M., additional, Petersen, Lyle R., additional, Petraitis, Vidmantas, additional, Pham, Luu-Ly, additional, Picado, Albert, additional, Pilatz, Adrian, additional, Pilmis, Benoit, additional, Pinazo, María-Jesús, additional, Pletz, Mathias W., additional, Pogue, Jason M., additional, Polgreen, Evelyn L., additional, Polgreen, Philip M., additional, Posfay-Barbe, Klara M., additional, Powderly, William G., additional, Presti, Rachel, additional, Prod'hom, Guy, additional, Puolakkainen, Mirja, additional, Quinn, Thomas C., additional, Raoult, Didier, additional, Razonable, Raymund R., additional, Read, Robert C., additional, Redfield, Robert R., additional, Rentenaar, Rob J., additional, Reynolds, Steven J., additional, Ribi, Camillo, additional, Richardson, Malcolm D., additional, Ritter, Michele L., additional, Roch, Antoine, additional, Rockstroh, Jürgen Kurt, additional, Rojek, Amanda, additional, Romero, José R., additional, Rooijakkers, Suzan H.M., additional, Rosenbluth, Daniel, additional, Rosenzweig, Sergio D., additional, Rossolini, Gian Maria, additional, Rubinstein, Ethan, additional, Ryan, Greg, additional, Safren, Steven A., additional, Sahasrabuddhe, Vikrant V., additional, Saikku, Pekka A.I., additional, Sajadi, Mohammad M., additional, Salvaggio, Michelle R., additional, Santos, Carlos A.Q., additional, Satlin, Michael J., additional, Schaeffer, Anthony J., additional, Schimmer, Christoph, additional, Schooley, Robert T., additional, Schumacher, Richard F., additional, Sha, Beverly E., additional, Shapiro, Daniel S., additional, Sheehan, Gerard, additional, Shlaes, David M., additional, Shoham, Shmuel, additional, Simmons, Cameron P., additional, Simon, Dennis W., additional, Simon, Matthew S., additional, Simonsen, Kari A., additional, Slack, Mary P.E., additional, Smith, Tyrel T., additional, Sobel, Jack D., additional, Souli, Maria, additional, Sridhar, Shruti, additional, Steckelberg, James M., additional, Stevens, Dennis L., additional, Strah, Heather, additional, Sturm, A. Willem, additional, Sungkanuparph, Somnuek, additional, Tabrizi, Sarah J., additional, Tacconelli, Evelina, additional, Tan, Chen Sabrina, additional, Taplitz, Randy A., additional, Thomas, Guillemette, additional, Thomas, Lora D., additional, Thuny, Franck, additional, Thwaites, Guy, additional, Tissot, Frederic, additional, Tønjum, Tone, additional, Torriani, Francesca J., additional, Toso, Christian, additional, Tulkens, Paul M., additional, Tunkel, Allan R., additional, Turner, Claire E., additional, Ustianowski, Andrew P., additional, van Bambeke, Françoise, additional, van Crevel, Reinout, additional, van de Beek, Diederik, additional, van Delden, Christian, additional, van der Eerden, Menno M., additional, van der Meer, Jos W.M., additional, van der Poll, Tom, additional, van Ingen, Jakko, additional, van Putten, Jos, additional, Vaudaux, Bernard P., additional, Vermund, Sten H., additional, Viscidi, Raphael P., additional, Visvanathan, Kumar, additional, Visvesvara, Govinda S., additional, von Seidlein, Lorenz, additional, Wagenlehner, Florian M.E., additional, Wald, Anna, additional, Walsh, Thomas J., additional, Warhurst, David C., additional, Warnock, David W., additional, Warrell, David A., additional, Warrell, Mary J., additional, Warris, Adilia, additional, Watkins, Richard R., additional, Weatherall, David J., additional, Weber, Rainer, additional, Weidner, Wolfgang, additional, White, Jonathan R., additional, White, Peter J., additional, Whitehorn, James, additional, Whitley, Richard J., additional, Whitty, Christopher J.M., additional, Wiersinga, Willem Joost, additional, Wilcox, Mark H., additional, Williams, Thomas N., additional, Wilson, Cara C., additional, Wilson, Mary Elizabeth, additional, Wisplinghoff, Hilmar, additional, Wood, Robin, additional, Wunderink, Richard G., additional, Wyles, David, additional, Yang, Zhi-Tao, additional, Yoder, Jonathan S., additional, Zaidi, Najam A., additional, Zimmer, Andrea J., additional, Zuckerman, Jane N., additional, and Zumla, Alimuddin, additional
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- 2017
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25. Longitudinal variability in the urinary microbiota of healthy premenopausal women and the relation to neighboring microbial communities: A pilot study
- Author
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Biehl, Lena M., Farowski, Fedja, Hilpert, Catharina, Nowag, Angela, Kretzschmar, Anne, Jazmati, Nathalie, Tsakmaklis, Anastasia, Wieters, Imke, Khodamoradi, Yascha, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Biehl, Lena M., Farowski, Fedja, Hilpert, Catharina, Nowag, Angela, Kretzschmar, Anne, Jazmati, Nathalie, Tsakmaklis, Anastasia, Wieters, Imke, Khodamoradi, Yascha, Wisplinghoff, Hilmar, and Vehreschild, Maria J. G. T.
- Abstract
Background The understanding of longitudinal changes in the urinary microbiota of healthy women and its relation to intestinal microbiota is limited. Methods From a cohort of 15 premenopausal women without known urogenital disease or current symptoms, we collected catheter urine (CU), vaginal and periurethral swabs, and fecal samples on four visits over six months. Additionally, ten participants provided CU and midstream urine (MU) to assess comparability. Urine was subjected to expanded culture. 16S rRNA gene sequencing was performed on all urine, fecal, and selected vaginal and periurethral samples. Sequence reads were processed (DADA2 pipeline) and analyzed using QIIME 2 and R. Results Relative abundances of urinary microbiota were variable over 6-18 months. The degree of intraindividual variability of urinary microbiota was higher than that found in fecal samples. Still, nearly half of the observed beta diversity of all urine samples could be attributed to differences between volunteers (R-2 = 0.48, p = 0.001). After stratification by volunteer, time since last sexual intercourse was shown to be a factor significantly contributing to beta diversity (R-2 = 0.14, p = 0.001). We observed a close relatedness of urogenital microbial habitats and a clear distinction from intestinal microbiota in the overall betadiversity analysis. Microbiota compositions derived from MU differed only slightly from CU compositions. Within this analysis of low-biomass samples, we identified contaminating sequences potentially stemming from sequencing reagents. Conclusions Results from our longitudinal cohort study confirmed the presence of a rather variable individual urinary microbiota in premenopausal women. These findings from catheter urine complement previous observations on temporal dynamics in voided urine. The higher intraindividual variability of urinary microbiota as compared to fecal microbiota will be a challenge for future studies investigating associations with urogenital
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- 2022
26. Limited protection against SARS-CoV-2 infection and virus transmission after mRNA vaccination
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Hsu, Lea, Wisplinghoff, Hilmar, Kossow, Annelene, Hurrass, Julia, Wiesmueller, Gerhard A., Grune, Barbara, Hoffmann, Dennis, Luesebrink, Jessica, Demuth, Sabrina, Schildgen, Oliver, Schildgen, Verena, Hsu, Lea, Wisplinghoff, Hilmar, Kossow, Annelene, Hurrass, Julia, Wiesmueller, Gerhard A., Grune, Barbara, Hoffmann, Dennis, Luesebrink, Jessica, Demuth, Sabrina, Schildgen, Oliver, and Schildgen, Verena
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- 2022
27. Invasive Trichoderma spp. infections: clinical presentation and outcome of cases from the literature and the FungiScope(R) registry
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Sal, Ertan, Stemler, Jannik, Salmanton-Garcia, Jon, Falces-Romero, Iker, Kredics, Laszlo, Meyer, Elisabeth, Wuerstl, Benjamin, Lass-Floerl, Cornelia, Racil, Zdenek, Klimko, Nikolay, Cesaro, Simone, Kindo, Anupma Jyoti, Wisplinghoff, Hilmar, Koehler, Philipp, Cornely, Oliver A., Seidel, Danila, Sal, Ertan, Stemler, Jannik, Salmanton-Garcia, Jon, Falces-Romero, Iker, Kredics, Laszlo, Meyer, Elisabeth, Wuerstl, Benjamin, Lass-Floerl, Cornelia, Racil, Zdenek, Klimko, Nikolay, Cesaro, Simone, Kindo, Anupma Jyoti, Wisplinghoff, Hilmar, Koehler, Philipp, Cornely, Oliver A., and Seidel, Danila
- Abstract
Background Trichoderma spp. are filamentous fungi causing invasive fungal diseases in patients with haematological malignancies and in peritoneal dialysis patients. Objectives To analyse clinical presentation, predisposing factors, treatment and outcome of Trichoderma infections. Methods A systematic literature review was conducted for published cases of invasive Trichoderma infection in PubMed until December 2021 and by reviewing the included studies' references. Cases from the FungiScope(R) registry were added to a combined analysis. Results We identified 50 invasive infections due to Trichoderma species, including 11 in the FungiScope(R) registry. The main underlying conditions were haematological malignancies in 19 and continuous ambulatory peritoneal dialysis (CAPD) in 10 cases. The most prevalent infection sites were lung (42%) and peritoneum (22%). Systemic antifungal therapy was administered in 42 cases (84%), mostly amphotericin B (n = 27, lipid-based formulation 13/27) and voriconazole in 15 cases (30%). Surgical interventions were performed in 13 cases (26%). Overall mortality was 48% (n = 24) and highest for allogeneic HSCT and solid organ transplantation (SOT) recipients [80% (4/5) and 77% (7/9), respectively]. In patients treated with amphotericin B, voriconazole and caspofungin, mortality was 55% (15/27), 46% (7/15) and 28% (2/7), respectively. Three out of four patients treated with a combination therapy of voriconazole and caspofungin survived. Conclusions Despite treatment with antifungal therapies and surgery, invasive Trichoderma infections are life-threatening complications in immunocompromised patients, especially after HSCT and SOT. In addition, Trichoderma spp. mainly affect the lungs in patients with haematological malignancies and the peritoneum in CAPD patients.
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- 2022
28. Dietary omega-6/omega-3 ratio is not associated with gut microbiota composition and disease severity in patients with nonalcoholic fatty liver disease
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Heinzer, Kathrin, Lang, Sonja, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Martin, Anna, Nowag, Angela, Kretzschmar, Anne, Scholz, Claus Juergen, Roderburg, Christoph, Mohr, Raphael, Tacke, Frank, Kasper, Philipp, Goeser, Tobias, Steffen, Hans -Michael, Demir, Muenevver, Heinzer, Kathrin, Lang, Sonja, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Martin, Anna, Nowag, Angela, Kretzschmar, Anne, Scholz, Claus Juergen, Roderburg, Christoph, Mohr, Raphael, Tacke, Frank, Kasper, Philipp, Goeser, Tobias, Steffen, Hans -Michael, and Demir, Muenevver
- Abstract
In this cross-sectional study, we hypothesized that a high dietary ratio of omega-6 (n-6) to omega-3 (n-3) fatty acids could be associated with an altered gut bacterial composition and with the disease severity in patients with nonalcoholic fatty liver disease (NAFLD). A total of 101 NAFLD patients were included in the study, of which 63 underwent a liver biopsy. All 101 patients completed a 14-day food and activity record. Ebispro 2016 professional software was used to calculate individual macronutrients and micronutrients consumed. Patients were grouped into 3 quantiles (Q) according to a low (Q1: < 6.1, n = 34), moderate (Q2: 6.1-7.8, n = 33), or high (Q3: > 7.8, n = 34) dietary n-6/n-3 ratio. Stool samples were analyzed using 16S rRNA gene sequencing. Spearman correlation coefficients and principal coordinate analysis were used to detect differences in the bacterial composition of the gut microbiota. The me-dian dietary n-6/n-3 ratio of all patients was 6.7 (range, 3.1-14.9). No significant associations between the dietary n-6/n-3 ratio and the gut microbiota composition or disease severity were observed. However, the abundance of specific bacteria such as Catenibacterium or Lacto-bacillus ruminis were found to be positively correlated and the abundance of Clostridium were negatively correlated with dietary n-6 fatty acid intake. The results indicate that a high di-etary n-6/n-3 ratio is probably not a highly relevant factor in the pathogenesis of human NAFLD. Further studies are needed to clarify the importance of interactions between gut bacterial taxa and n-6 fatty acids in the pathophysiology of NAFLD.(c) 2022 Elsevier Inc. All rights reserved.
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- 2022
29. Longitudinal variability in the urinary microbiota of healthy premenopausal women and the relation to neighboring microbial communities: a pilot study
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Biehl, Lena Maria, Farowski, Fedja, Hilpert, Catharina, Nowag, Angela, Kretzschmar, Anne, Jazmati, Nathalie Rihab, Tsakmaklis, Anastasia, Wieters, Imke, Khodamoradi, Yascha, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Biehl, Lena Maria, Farowski, Fedja, Hilpert, Catharina, Nowag, Angela, Kretzschmar, Anne, Jazmati, Nathalie Rihab, Tsakmaklis, Anastasia, Wieters, Imke, Khodamoradi, Yascha, Wisplinghoff, Hilmar, and Vehreschild, Maria J. G. T.
- Abstract
Background: The understanding of longitudinal changes in the urinary microbiota of healthy women and its relation to intestinal microbiota is limited. Methods: From a cohort of 15 premenopausal women without known urogenital disease or current symptoms, we collected catheter urine (CU), vaginal and periurethral swabs, and fecal samples on four visits over six months. Additionally, ten participants provided CU and midstream urine (MU) to assess comparability. Urine was subjected to expanded culture. 16S rRNA gene sequencing was performed on all urine, fecal, and selected vaginal and periurethral samples. Sequence reads were processed (DADA2 pipeline) and analyzed using QIIME 2 and R. Results: Relative abundances of urinary microbiota were variable over 6–18 months. The degree of intraindividual variability of urinary microbiota was higher than that found in fecal samples. Still, nearly half of the observed beta diversity of all urine samples could be attributed to differences between volunteers (R2 = 0.48, p = 0.001). After stratification by volunteer, time since last sexual intercourse was shown to be a factor significantly contributing to beta diversity (R2 = 0.14, p = 0.001). We observed a close relatedness of urogenital microbial habitats and a clear distinction from intestinal microbiota in the overall betadiversity analysis. Microbiota compositions derived from MU differed only slightly from CU compositions. Within this analysis of low-biomass samples, we identified contaminating sequences potentially stemming from sequencing reagents. Conclusions: Results from our longitudinal cohort study confirmed the presence of a rather variable individual urinary microbiota in premenopausal women. These findings from catheter urine complement previous observations on temporal dynamics in voided urine. The higher intraindividual variability of urinary microbiota as compared to fecal microbiota will be a challenge for future studies investigating associations with urogenita
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- 2022
30. Cumulative Incidence of SARS-CoV-2 in Healthcare Workers at a General Hospital in Germany during the Pandemic—A Longitudinal Analysis
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Platten, Martin, primary, Nienhaus, Albert, additional, Peters, Claudia, additional, Cranen, Rita, additional, Wisplinghoff, Hilmar, additional, Kersten, Jan Felix, additional, Bach, Alexander Daniel, additional, and Michels, Guido, additional
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- 2022
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31. Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children
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Dewald, Felix, primary, Suárez, Isabelle, additional, Johnen, Ronja, additional, Grossbach, Jan, additional, Moran-Tovar, Roberto, additional, Steger, Gertrud, additional, Joachim, Alexander, additional, Rubio, Gibran Horemheb, additional, Fries, Mira, additional, Behr, Florian, additional, Kley, Joao, additional, Lingnau, Andreas, additional, Kretschmer, Alina, additional, Gude, Carina, additional, Beazes-Flores, Guadelupe, additional, del Valle, David Laveaga, additional, Roblero-Hernandez, Alberto, additional, Magana-Cerino, Jesus, additional, Hernandez, Adriana Torres, additional, Ruiz-Quinones, Jesus, additional, Schega, Konstantin, additional, Linne, Viktoria, additional, Junker, Lena, additional, Wunsch, Marie, additional, Heger, Eva, additional, Knops, Elena, additional, Di Cristanziano, Veronica, additional, Meyer, Meike, additional, Hünseler, Christoph, additional, Weber, Lutz T., additional, Lüers, Jan-Christoffer, additional, Quade, Gustav, additional, Wisplinghoff, Hilmar, additional, Tiemann, Carsten, additional, Zotz, Rainer, additional, Jomaa, Hassan, additional, Pranada, Arthur, additional, Herzum, Ileana, additional, Cullen, Paul, additional, Schmitz, Franz-Josef, additional, Philipsen, Paul, additional, Kirchner, Georg, additional, Knabbe, Cornelius, additional, Hellmich, Martin, additional, Buess, Michael, additional, Wolff, Anna, additional, Kossow, Annelene, additional, Niessen, Johannes, additional, Jeworutzki, Sebastian, additional, Schräpler, Jörg-Peter, additional, Lässig, Michael, additional, Dötsch, Jörg, additional, Fätkenheuer, Gerd, additional, Kaiser, Rolf, additional, Beyer, Andreas, additional, Rybniker, Jan, additional, and Klein, Florian, additional
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- 2022
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32. Longitudinal variability in the urinary microbiota of healthy premenopausal women and the relation to neighboring microbial communities: A pilot study
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Biehl, Lena M., primary, Farowski, Fedja, additional, Hilpert, Catharina, additional, Nowag, Angela, additional, Kretzschmar, Anne, additional, Jazmati, Nathalie, additional, Tsakmaklis, Anastasia, additional, Wieters, Imke, additional, Khodamoradi, Yascha, additional, Wisplinghoff, Hilmar, additional, and Vehreschild, Maria J. G. T., additional
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- 2022
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33. Limited protection against SARS-CoV-2 infection and virus transmission after mRNA vaccination
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Hsu, Lea, primary, Wisplinghoff, Hilmar, additional, Kossow, Annelene, additional, Hurraß, Julia, additional, Wiesmüller, Gerhard A., additional, Grüne, Barbara, additional, Hoffmann, Dennis, additional, Lüsebrink, Jessica, additional, Demuth, Sabrina, additional, Schildgen, Oliver, additional, and Schildgen, Verena, additional
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- 2022
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34. Organmykosen
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Wisplinghoff, Hilmar, primary and Cornely, Oliver A., additional
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- 2015
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35. SARS-CoV-2 Infection in Fully Vaccinated Individuals of Old Age Strongly Boosts the Humoral Immune Response
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Müller, Lisa, primary, Andrée, Marcel, additional, Ostermann, Philipp Niklas, additional, Jazmati, Nathalie, additional, Flüh, Greta, additional, Fischer, Johannes C., additional, Bölke, Edwin, additional, Heger, Eva, additional, Vanshylla, Kanika, additional, Klein, Florian, additional, Wisplinghoff, Hilmar, additional, Schaal, Heiner, additional, Drexler, Ingo, additional, Walker, Andreas, additional, Adams, Ortwin, additional, and Timm, Jörg, additional
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- 2021
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36. Cumulative incidence of SARS-CoV-2 infections in health workers in Germany
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Nienhaus, Albert, primary, Kersten, Jan Felix, additional, Platten, Martin, additional, Cranen, Rita, additional, Peters, Claudia, additional, Wisplinghoff, Hilmar, additional, Bach, Alexander Daniel, additional, and Michels, Guido, additional
- Published
- 2021
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37. Association between the dietary regimen and infection-related complications in neutropenic high-risk patients with cancer
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Jakob, Carolin E.M., primary, Classen, Annika Y., additional, Stecher, Melanie, additional, Engert, Andreas, additional, Freund, Meike, additional, Hamprecht, Axel, additional, Jazmati, Nathalie, additional, Wisplinghoff, Hilmar, additional, Hallek, Michael, additional, Cornely, Oliver A., additional, and Vehreschild, Jörg J., additional
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- 2021
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38. Matched-paired analysis of patients treated for invasive mucormycosis: standard treatment versus posaconazole new formulations (MoveOn)
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Salmanton-García, Jon, Seidel, Danila, Koehler, Philipp, Mellinghoff, Sibylle, Herbrecht, Raoul, Klimko, Nikolai, Ráčil, Zdeněk, Falces-Romero, Iker, Ingram, Paul, Benítez-Peñuela, Miguel-Ángel, Rodríguez, José Yesid, Desoubeaux, Guillaume, Barać, Aleksandra, García-Vidal, Carolina, Hoenigl, Martin, Mehta, Sanjay, Cheng, Matthew, Klyasova, Galina, Heinz, Werner, Iqbal, Nousheen, Krause, Robert, Ostermann, Helmut, Penack, Olaf, Schalk, Enrico, Sheppard, Donald, Willinger, Birgit, Wisplinghoff, Hilmar, Vehreschild, J Janne, Cornely, Oliver, Vehreschild, Maria, Khedr, Reham Abdelaziz, Arencibia-Núñez, Alberto, Avilés-Robles, Martha, Banke, Ingo, Basher, Ariful, Benachinamardi, Keertilaxmi, Bertz, Harmut, Chakrabarti, Arunaloke, Drgona, Lubos, García-Martínez, Jesús, García-Rodríguez, Julio, Gräber, Sandra, Härter, Georg, Klein, Michael, Kouba, Michal, Lee, Dong-Gun, Le Govic, Yohann, Leo, Fabian, Maertens, Johan, Maschmeyer, Georg, Meintker, Lisa, Mo, Xiao-Dong, Müller, Lena-Katharina, Müller, Nicolas, Nel, Jeremy Stephen, Novák, Jan, Patel, Atul, Pfäfflin, Frieder, Pozo-Laderas, Juan-Carlos, Puerta-Alcalde, Pedro, Rodríguez-Guardado, Azucena, Schroers, Roland, Shekar, Vandana, Shenoi, Susan, Silling, Gerda, Vinh, Donald, Waizel-Haiat, Salomón, Yee Yee, Mandy Yap, Prakash, Peralam Yegneswaran, Žák, Pavel, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Service d'Hématologie, CHU Strasbourg, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Medicine, Medical University Graz, Schwerpunkt Infektiologie, Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg, Department of Haematology and Oncology, Medical Clinic III, University Hospital Munich—Großhadern, Ludwig Maximilian University, Klinik für Hämatologie und Onkologie, Charité, Campus Benjamin Franklin, Department of Haematology/Oncology, Magdeburg University Hospital, McGill University = Université McGill [Montréal, Canada], Medizinische Universität Wien = Medical University of Vienna, University Hospital of Cologne [Cologne], Postgraduate Institute of Medical Education and Research, Laboratoire de psychologie cognitive (LPC), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Anofel Cryptosporidium National Network, Department of Hematology, University Hospital Gasthuisberg, Medizinische Klinik, Hämatologie und Onkologie, Klinikum Ernst von Bergmann, Real Expression Artificial Life (IRIT-REVA), Institut de recherche en informatique de Toulouse (IRIT), Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Medizinische Klinik A des Universitätsklinikums Münster, Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), University Hospital Gasthuisberg [Leuven], Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Toulouse Mind & Brain Institut (TMBI), Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), and Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)
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Male ,0301 basic medicine ,Posaconazole ,Antifungal Agents ,[SDV]Life Sciences [q-bio] ,Gastroenterology ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Amphotericin B ,Pharmacology (medical) ,Prospective Studies ,Registries ,030212 general & internal medicine ,Child ,Prospective cohort study ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,ComputingMilieux_MISCELLANEOUS ,Antiinfective agent ,Standard treatment ,Middle Aged ,3. Good health ,Infectious Diseases ,Tolerability ,Child, Preschool ,Mucorales ,Female ,medicine.drug ,Adult ,Microbiology (medical) ,medicine.medical_specialty ,Adolescent ,Drug Compounding ,Matched-Pair Analysis ,030106 microbiology ,Young Adult ,03 medical and health sciences ,Pharmacokinetics ,Internal medicine ,parasitic diseases ,medicine ,Humans ,Mucormycosis ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,[INFO]Computer Science [cs] ,Aged ,Pharmacology ,business.industry ,Infant, Newborn ,Infant ,Triazoles ,medicine.disease ,business ,MESH: amphotericin b, antifungal agents, cancer, kidney failure, mucormycosis, surgical procedures, operative, suspensions, mortality, posaconazole ,Invasive Fungal Infections - Abstract
Background First-line antifungal treatment for invasive mucormycosis (IM) consists of liposomal amphotericin B. Salvage treatment options are limited and often based on posaconazole oral suspension. With the approval of posaconazole new formulations, patients could benefit from improved pharmacokinetics, safety and tolerability. Objectives Our aim was to assess the effectiveness of posaconazole new formulations for IM treatment. Methods We performed a case-matched analysis with proven or probable IM patients from the FungiScope® Registry. First-line posaconazole new formulations (1st-POSnew) and first-line amphotericin B plus posaconazole new formulations (1st-AMB+POSnew) cases were matched with first-line amphotericin B-based (1st-AMB) treatment controls. Salvage posaconazole new formulations (SAL-POSnew) cases were matched with salvage posaconazole oral suspension (SAL-POSsusp) controls. Each case was matched with up to three controls (based on severity, haematological/oncological malignancy, surgery and/or renal dysfunction). Results Five patients receiving 1st-POSnew, 18 receiving 1st-AMB+POSnew and 22 receiving SAL-POSnew were identified. By day 42, a favourable response was reported for 80.0% (n = 4/5) of patients receiving 1st-POSnew, for 27.8% (n = 5/18) receiving 1st-AMB+POSnew and for 50.0% (n = 11/22) receiving SAL-POSnew. Day 42 all-cause mortality of patients receiving posaconazole new formulations was lower compared with controls [20.0% (n = 1/5) in 1st-POSnew versus 53.3% (n = 8/15) in 1st-AMB; 33.3% (n = 6/18) in 1st-AMB+POSnew versus 52.0% (n = 26/50) in 1st-AMB; and 0.0% (n = 0/22) in SAL-POSnew versus 4.4% (n = 2/45) in SAL-POSsusp]. Conclusions Posaconazole new formulations were effective in terms of treatment response and associated mortality of IM. While posaconazole new formulations may be an alternative for treatment of IM, the limited sample size of our study calls for a cautious interpretation of these observations.
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- 2019
39. A cost and resource utilization analysis of micafungin bridging for hemato-oncological high-risk patients undergoing allogeneic stem cell transplantation
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Heimann, Sebastian M., Vehreschild, Maria J.G.T., Cornely, Oliver A., Franke, Bernd, von Bergwelt-Baildon, Michael, Wisplinghoff, Hilmar, Kron, Florian, Scheid, Christoph, and Vehreschild, Jörg J.
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- 2015
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40. Feasibility and effectiveness of posaconazole prophylaxis in combination with micafungin bridging for patients undergoing allogeneic stem cell transplantation: A 6-yr analysis from the cologne cohort for neutropenic patients
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Vehreschild, Maria J. G. T., von Bergwelt-Baildon, Michael, Tran, Liliane, Shimabukuro-Vornhagen, Alexander, Wisplinghoff, Hilmar, Bangard, Christopher, Cornely, Oliver Andreas, and Vehreschild, Jörg Janne
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- 2014
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41. SARS-CoV-2, CT-Values, and Infectivity—Conclusions to Be Drawn from Side Observations
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Platten, Martin, primary, Hoffmann, Dennis, additional, Grosser, Roger, additional, Wisplinghoff, Fabian, additional, Wisplinghoff, Hilmar, additional, Wiesmüller, Gerhard, additional, Schildgen, Oliver, additional, and Schildgen, Verena, additional
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- 2021
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42. SARS-CoV-2, CT-Values, and Infectivity : Conclusions to Be Drawn from Side Observations
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Platten, Martin, Hoffmann, Dennis, Grosser, Roger, Wisplinghoff, Fabian, Wisplinghoff, Hilmar, Wiesmüller, Gerhard, Schildgen, Oliver, and Schildgen, Verena
- Abstract
Viruses / Molecular Diversity Preservation International 13(8), 1459 (2021). doi:10.3390/v13081459 special issue: "Burden of COVID-19 in Different Countries / Topic Editors: Dr. Dimitrios Paraskevis, Dr. Maria Yavropoulou, Prof. Dr. Sotirios Tsiodras", Published by MDPI, Basel
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- 2021
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43. Clinical and pharmacoeconomic evaluation of antifungal prophylaxis with continuous micafungin in patients undergoing allogeneic stem cell transplantation: A six-year cohort analysis
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Wingen-Heimann, Sebastian M., Cornely, Oliver A., Vehreschild, Maria J. G. T., Wisplinghoff, Hilmar, Franke, Bernd, Schons, Max, von Bergwelt-Baildon, Michael, Scheid, Christof, Vehreschild, Joerg Janne, Wingen-Heimann, Sebastian M., Cornely, Oliver A., Vehreschild, Maria J. G. T., Wisplinghoff, Hilmar, Franke, Bernd, Schons, Max, von Bergwelt-Baildon, Michael, Scheid, Christof, and Vehreschild, Joerg Janne
- Abstract
Background Patients undergoing allogeneic stem cell transplantation (aSCT) are at high risk to develop an invasive fungal disease (IFD). Optimisation of antifungal prophylaxis strategies may improve patient outcomes and reduce treatment costs. Objectives To analyse the clinical and economical impact of using continuous micafungin as antifungal prophylaxis. Patients/Methods We performed a single-centre evaluation comparing patients who received either oral posaconazole with micafungin as intravenous bridging as required (POS-MIC) to patients who received only micafungin (MIC) as antifungal prophylaxis after aSCT. Epidemiological, clinical and direct treatment cost data extracted from the Cologne Cohort of Neutropenic Patients (CoCoNut) were analysed. Results Three hundred and thirteen patients (97 and 216 patients in the POS-MIC and MIC groups, respectively) were included into the analysis. In the POS-MIC and MIC groups, median overall length of stay was 42 days (IQR: 35-52 days) vs 40 days (IQR: 35-49 days; p = .296), resulting in median overall costs of euro42,964 (IQR: euro35,040-euro56,348) vs euro43,291 (IQR: euro37,281 vs euro51,848; p = .993), respectively. Probable/proven IFD in the POS-MIC and MIC groups occurred in 5 patients (5%) vs 3 patients (1%; p = .051), respectively. The Kaplan-Meier analysis showed improved outcome of patients in the MIC group at day 100 (p = .037) and day 365 (p < .001) following aSCT. Conclusions Our study results demonstrate improved outcomes in the MIC group compared with the POS-MIC group, which can in part be explained by a tendency towards less probable/proven IFD. Higher drug acquisition costs of micafungin did not translate into higher overall costs.
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- 2021
44. Association between the dietary regimen and infection-related complications in neutropenic high-risk patients with cancer
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Jakob, Carolin E. M., Classen, Annika Y., Stecher, Melanie, Engert, Andreas, Freund, Meike, Hamprecht, Axel, Jazmati, Nathalie, Wisplinghoff, Hilmar, Hallek, Michael, Cornely, Oliver A., Vehreschild, Joerg J., Jakob, Carolin E. M., Classen, Annika Y., Stecher, Melanie, Engert, Andreas, Freund, Meike, Hamprecht, Axel, Jazmati, Nathalie, Wisplinghoff, Hilmar, Hallek, Michael, Cornely, Oliver A., and Vehreschild, Joerg J.
- Abstract
Background: Many haematology/oncology departments still provide a germ-free diet for neutropenic patients (neutropenic diet, ND) to minimise pathogen exposure, even though evidence on benefits is missing. We analysed the effects of a standard diet (SD) in neutropenic high-risk patients with cancer while focussing on infection-related outcomes. Patients and methods: Based on the Cologne Cohort of Neutropenic Patients, we conducted a propensity score-matched case-control study in haematological/oncological patients with a period of neutropenia longer than five days treated at our department between January 2004 and December 2012 (implementation of SD in January 2008). We assessed the association between an SD and selected infection-related end-points in an adjusted multivariable regression model and time-to-event analysis. Results: In total, 2086 neutropenic episodes (1043 per diet group) were included into analysis. The median days of neutropenia were 9 (interquartile range 7-16). The adjusted multivariable model revealed no association between the SD and severity and persistence of fever, death within 28 days, antibiotic treatment and weight loss >3 kg and a non-significant adjusted association between SD and duration of antibiotic treatment and blood stream infections. There was a significant association between SD and incidence of diarrhoea (odds ratio [OR], 0.55; 95% confidence interval [CI], 0.45-0.68; P < 0.001), nausea (OR, 0.53; 95% CI, 0.43-0.66; P < 0.001) and weight loss >1 kg (OR, 0.93; 95% CI, 0.89-0.98; P Z 0.002) with fewer events in SD than in the ND group. The hazard ratios of SD for the analysed end-points were nonsignificant. Conclusion: In our study, the implementation of an SD for high-risk neutropenic patients with cancer was safe regarding infection-related end-points. (C) 2021 Elsevier Ltd. All rights reserved.
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- 2021
45. Prevalence of SARS-CoV-2 in employees of a general hospital in Northrhine-Westphalia, Germany
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Platten, Martin, Cranen, Rita, Peters, Claudia, Wisplinghoff, Hilmar, Nienhaus, Albert, Bach, Alexander Daniel, Michels, Guido, Platten, Martin, Cranen, Rita, Peters, Claudia, Wisplinghoff, Hilmar, Nienhaus, Albert, Bach, Alexander Daniel, and Michels, Guido
- Abstract
Background We assessed the prevalence of SARS-CoV-2 in the staff of a general hospital in North-Rhine-Westphalia in a cross-sectional study. Method Employees (n = 1363) were offered a nasopharyngeal swab and serology for SARS-CoV-2. Additionally, employees completed a questionnaire about preexisting conditions, contacts with SARS-CoV- 2-positive individuals and COVID-19-specific symptoms. Results 1212 employees participated. 19 of 1363 (1.4 %) employees tested positive by PCR (3 within and 16 before the study). 40 (3.3 %) and 105 (8.6 %) had IgG and IgA, respectively, 32 (2.6 %) both IgG and IgA. Overall, 47 employees tested positive. In this group, most frequently reported symptoms were headache ( 56 %), fatigue (49 %), sore throat (49 %), and cough (46 %); fever was reported by 33 %. SARS-CoV-2-positive employees reported more frequently contact with COVID-19 cases (60.5 % vs. 37.3 %, p = 0.006). Employees testing positive only for IgA reported less symptoms. Conclusion Between 27.04. and 20.05.2020, 3.9 % of the employees working in a general hospital were tested positive for SARS-CoV-2. This proportion was lower than expected; possible explanations are the low level of endemic infection and the extensive, uniform in-house preventative measures.
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- 2021
46. SARS-CoV-2 Infection in Fully Vaccinated Individuals of Old Age Strongly Boosts the Humoral Immune Response
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Mueller, Lisa, Andree, Marcel, Ostermann, Philipp Niklas, Jazmati, Nathalie, Flueh, Greta, Fischer, Johannes C., Boelke, Edwin, Heger, Eva, Vanshylla, Kanika, Klein, Florian, Wisplinghoff, Hilmar, Schaal, Heiner, Drexler, Ingo, Walker, Andreas, Adams, Ortwin, Timm, Jorg, Mueller, Lisa, Andree, Marcel, Ostermann, Philipp Niklas, Jazmati, Nathalie, Flueh, Greta, Fischer, Johannes C., Boelke, Edwin, Heger, Eva, Vanshylla, Kanika, Klein, Florian, Wisplinghoff, Hilmar, Schaal, Heiner, Drexler, Ingo, Walker, Andreas, Adams, Ortwin, and Timm, Jorg
- Abstract
Prophylactic vaccination against SARS-CoV-2 is one of the most important measures to contain the COVID-19 pandemic. Recently, break-through infections following vaccination against this virus have been reported. Here, we describe the humoral immune response of break-through infections in fully vaccinated individuals of old age from an outbreak in a nursing home. In cooperation with the local health authority, blood samples from fully vaccinated and infected as well as fully vaccinated and uninfected residents of the nursing home were collected 4 weeks after the onset of the outbreak. The humoral immune response was determined in a neutralisation assay with replication-competent virus isolates and by a quantitative ELISA. In this outbreak a total of 23 residents and four health care workers were tested positive for SARS-CoV-2. Four residents were unvaccinated, including one with a severe course of disease who later severe disease course who later succumbed to infection. Despite their old age, all vaccinated residents showed no or only mild disease. Comparison of the humoral immune response revealed significantly higher antibody levels in fully vaccinated infected individuals compared to fully vaccinated uninfected individuals (p < 0.001). Notably, although only a minority of the vaccinated uninfected group showed neutralisation capacity against SARS-CoV-2, all vaccinated and infected individuals showed high-titre neutralisation of SARS-CoV-2 including the alpha and beta variant. Large SARS-CoV-2 outbreaks can occur in fully vaccinated populations, but seem to associate with mild disease. SARS-CoV-2 infection in fully vaccinated individuals is a strong booster of the humoral immune response providing enhanced neutralisation capacity against immune evasion variants.
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- 2021
47. Molecular epidemiological typing of Neisseria gonorrhoeae isolates identifies a novel association between genogroup G10557 (G7072) and decreased susceptibility to cefixime, Germany, 2014 to 2017
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Banhart, Sebastian, Jansen, Klaus, Buder, Susanne, Tamminga, Thalea, Calvignac-Spencer, Sebastien, Pilz, Tanja, Martini, Andrea, Dudareva, Sandra, Nikisins, Sergejs, Dehmel, Kerstin, Zuelsdorf, Gabriele, Guhl, Eva, Graeber, Ingeborg, Kohl, Peter K., Unemo, Magnus, Bremer, Viviane, Heuer, Dagmar, Back, Thomas, Berger, Anja, Chapot, le Saout, Valerie, Steinmann, Jörg, Eicke, Stephan, Friedrichs, Claudia, Groß, Andreas, Hagedorn, Hans-Jochen, Halfmann, Alexander, Hornei, Britt, Ignatius, Ralf, Korten, Simone, Sahly, Hany, Kozub-Witkowski, Elzbieta, Krämer, Sabine, Kühn, Margit, Liebetrau, Anke, Meyer, Thomas, Oberdorfer, Klaus, Pfüller, Roland, Ruckert, Caroline, Schwarz, Roman, Walch, Daniela, Mai, Madeleine, Wichelhaus, Thomas A., Wisplinghoff, Hilmar, and Wüppenhorst, Nicole
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0301 basic medicine ,medicine.medical_specialty ,Epidemiology ,business.industry ,030106 microbiology ,Public Health, Environmental and Occupational Health ,Medizin ,Azithromycin ,medicine.disease_cause ,03 medical and health sciences ,Exact test ,0302 clinical medicine ,Antibiotic resistance ,Interquartile range ,Virology ,Internal medicine ,medicine ,Ceftriaxone ,Neisseria gonorrhoeae ,030212 general & internal medicine ,Typing ,business ,Cefixime ,medicine.drug - Abstract
Background Emerging antimicrobial resistance (AMR) challenges gonorrhoea treatment and requires surveillance. Aim This observational study describes the genetic diversity of Neisseria gonorrhoeae isolates in Germany from 2014 to 2017 and identifies N. gonorrhoeae multi-antigen sequence typing (NG-MAST) genogroups associated with AMR or some patient demographics. Methods 1,220 gonococcal isolates underwent AMR testing and NG-MAST. Associations between genogroups and AMR or sex/age of patients were statistically assessed. Results Patients’ median age was 32 years (interquartile range: 25–44); 1,078 isolates (88.4%) originated from men. In total, 432 NG-MAST sequence types including 156 novel ones were identified, resulting in 17 major genogroups covering 59.1% (721/1,220) of all isolates. Genogroups G1407 and G10557 (G7072) were significantly associated with decreased susceptibility to cefixime (Kruskal–Wallis chi-squared: 549.3442, df: 16, p Conclusion AMR monitoring with molecular typing is important. Dual therapy (ceftriaxone plus azithromycin) recommended in 2014 in Germany, or only the ceftriaxone dose of this therapy, might have contributed to cefixime-resistant genogroups decreasing.
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- 2020
48. Invasive Scedosporium spp. and Lomentospora prolificans infections in pediatric patients: Analysis of 55 cases from FungiScope (R) and the literature
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Seidel, Danila, Hassler, Angela, Salmanton-Garcia, Jon, Koehler, Philipp, Mellinghoff, Sibylle C., Carlesse, Fabianne, Cheng, Matthew P., Falces-Romero, Iker, Herbrecht, Raoul, Jover Saenz, Alfredo, Klimko, Nikolai, Mares, Mihai, Lass-Floerl, Cornelia, Soler-Palacin, Pere, Wisplinghoff, Hilmar, Cornely, Oliver A., Pana, Zoi, Lehrnbecher, Thomas, Seidel, Danila, Hassler, Angela, Salmanton-Garcia, Jon, Koehler, Philipp, Mellinghoff, Sibylle C., Carlesse, Fabianne, Cheng, Matthew P., Falces-Romero, Iker, Herbrecht, Raoul, Jover Saenz, Alfredo, Klimko, Nikolai, Mares, Mihai, Lass-Floerl, Cornelia, Soler-Palacin, Pere, Wisplinghoff, Hilmar, Cornely, Oliver A., Pana, Zoi, and Lehrnbecher, Thomas
- Abstract
Objectives: Current knowledge on infections caused by Scedosporium spp. and Lomentospora prolificans in children is scarce. We therefore aim to provide an overview of risk groups, clinical manifestation and treatment strategies of these infections. Methods: Pediatric patients (age <18 years) with proven/probable Scedosporium spp. or L. prolificans infection were identified in PubMed and the FungiScope (R) registry. Data on diagnosis, treatment and outcome were collected. Results: Fifty-five children (median age 9 years [IQR: 5-14]) with invasive Scedosporium spp. (n = 33) or L. prolificans (n = 22) infection were identified between 1990 and 2019. Malignancy, trauma and near drowning were the most common risk factors. Infections were frequently disseminated. Most patients received systemic antifungal therapy, mainly voriconazole and amphotericin B, plus surgical treatment. Overall, day 42 mortality was 31%, higher for L. prolificans (50%) compared to Scedosporium spp. (18%). L. prolificans infection was associated with a shorter median survival time compared to Scedosporium spp. (6 days [IQR: 3-28] versus 61 days [IQR: 16-148]). Treatment for malignancy and severe disseminated infection were associated with particularly poor outcome (HR 8.33 [95% CI 1.35-51.40] and HR 6.12 [95% CI 1.52-24.66], respectively). Voriconazole use at any time and surgery for antifungal treatment were associated with improved clinical outcome (HR 0.33 [95% CI 0.11-0.99] and HR 0.09 [95% CI 0.02-0.40], respectively). Conclusions: Scedosporium spp. and L. prolificans infections in children are associated with high mortality despite comprehensive antifungal therapy. Voriconazole usage and surgical intervention are associated with successful outcome. (C) 2019 University Hospital of Cologne. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
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- 2020
49. High Protein Intake Is Associated With Histological Disease Activity in Patients With NAFLD
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Lang, Sonja, Martin, Anna, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Liu, Jinyuan, Krawczyk, Marcin, Nowag, Angela, Kretzschmar, Anne, Herweg, Jens, Schnabl, Bernd, Tu, Xin M., Lammert, Frank, Goeser, Tobias, Tacke, Frank, Heinzer, Kathrin, Kasper, Philipp, Steffen, Hans-Michael, Demir, Muenevver, Lang, Sonja, Martin, Anna, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Liu, Jinyuan, Krawczyk, Marcin, Nowag, Angela, Kretzschmar, Anne, Herweg, Jens, Schnabl, Bernd, Tu, Xin M., Lammert, Frank, Goeser, Tobias, Tacke, Frank, Heinzer, Kathrin, Kasper, Philipp, Steffen, Hans-Michael, and Demir, Muenevver
- Abstract
Overconsumption of carbohydrates and lipids are well known to cause nonalcoholic fatty liver disease (NAFLD), while the role of nutritional protein intake is less clear. In Western diet, meat and other animal products are the main protein source, with varying concentrations of specific amino acids. Whether the amount or composition of protein intake is associated with a higher risk for disease severity has not yet been examined. In this study, we investigated associations of dietary components with histological disease activity by analyzing detailed 14-day food records in a cohort of 61 patients with biopsy-proven NAFLD. Furthermore, we used 16S ribosomal RNA gene sequencing to detect associations with different abundances of the gut microbiota with dietary patterns. Patients with definite nonalcoholic steatohepatitis (NAFLD activity score of 5-8 on liver biopsy) had a significantly higher daily relative intake of protein compared with patients with a NAFLD activity score of 0-4 (18.0% vs. 15.8% of daily protein-based calories, P = 0.018). After adjustment for several potentially confounding factors, a higher protein intake (>= 17.3% of daily protein-based calories) remained associated with definite nonalcoholic steatohepatitis, with an odds ratio of 5.09 (95% confidence interval 1.22-21.25, P = 0.026). This association was driven primarily by serine, glycine, arginine, proline, phenylalanine, and methionine. A higher protein intake correlated with a lower Bacteroides abundance and an altered abundance of several other bacterial taxa. Conclusion: A high protein intake was independently associated with more active and severe histological disease activity in patients with NAFLD. Further studies are needed to investigate the potential harmful role of dietary amino acids on NAFLD, with special attention to meat as their major source.
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- 2020
50. Phenotyping non-alcoholic fatty liver disease by the gut microbiota: Ready for prime time?
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Demir, Muenevver, Lang, Sonja, Martin, Anna, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Krawczyk, Marcin, Nowag, Angela, Scholz, Claus Jurgen, Kretzschmar, Anne, Roderburg, Christoph, Lammert, Frank, Goeser, Tobias, Kasper, Philipp, Steffen, Hans-Michael, Demir, Muenevver, Lang, Sonja, Martin, Anna, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Krawczyk, Marcin, Nowag, Angela, Scholz, Claus Jurgen, Kretzschmar, Anne, Roderburg, Christoph, Lammert, Frank, Goeser, Tobias, Kasper, Philipp, and Steffen, Hans-Michael
- Abstract
Background and Aim Several studies observed alterations in the gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD). However, analyzed patient populations and methods strongly differ among these studies. The aim of this study was to prove the reproducibility of published results and to provide a detailed overview of all findings in our NAFLD cohort using next generation sequencing methods. Methods The individual taxonomic microbiota composition of fecal samples from 90 NAFLD patients and 21 healthy controls was analyzed using 16S rRNA gene sequencing. Study participants were grouped according to their disease stage and compared regarding their gut microbiota composition. Studies were identified from PubMed listed publications, and the results were compared with the findings in our cohort. Results Results from 13 identified studies were compared with our data. A decreased abundance of the Bacteroidetes and Ruminococcaceae as well as an increased abundance of Lactobacillaceae and Veillonellaceae and Dorea were the most frequently reported changes among NAFLD patients in 4/13, 5/13, 4/13, 2/13, and 3/13 studies, respectively. Even though these alterations in the gut microbiota composition were also observed in our patient cohort, the majority of published differences could not be reproduced, neither in our own nor in other NAFLD cohort studies. Conclusion Despite repeatedly reproduced abundance patterns of specific bacteria, the heterogeneous study results did not reveal a consistent disease specific gut microbiota signature. Further prospective studies with homogenous patient cohorts and standardized methods are necessary to phenotype NAFLD by the gut microbiota.
- Published
- 2020
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