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Additional file 2. Additional Results and Additional Figures

FCA Consortium: Stein Aerts, Devika Agarwal, Yasir Ahmed-Braimah, Aaron M. Allen, Michelle Arbeitman, Majd M. Ariss, Jordan Augsburger, Kumar Ayush, Catherine C. Baker, Torsten Banisch, Cameron W. Berry, Katja Birker, Rolf Bodmer, Benjamin Bolival, Susanna E. Brantley, Maria Brbić, Julie A. Brill, Nora C. Brown, Katja Brueckner, Norene A. Buehner, Xiaoyu Tracy Cai, Rita Cardoso-Figueiredo, Zita Carvalho-Santos, Casares,Fernando, Amy Chang, Thomas R. Clandinin, Sheela Crasta, Fabrice P. A. David, Kristofer Davie4, Bart Deplancke, Claude Desplan44, Angela M. Detweiler, Darshan B. Dhakan, Stephen DiNardo, Erika Donà, Julian A. T. Dow, Stefanie Engert, Swann Floc’hlay, Margaret T. Fuller, Anthony Galenza, Vincent Gardeux, Nancy George, Amanda J. González-Segarra, Stephen F. Goodwin, Andrew K. Groves, Samantha Gumbin Yanmeng Guo, Devon E. Harris, Yael Heifetz, Stephen L. Holtz, Felix Horns, Bruno Hudry, Ruei-Jiun Hung, Yuh Nung Jan, Jasper Janssens, Heinrich Jasper, Jacob S. Jaszczak, Gregory S. X. E. Jefferis, Robert C. Jones, Jim Karkanias, Timothy L. Karr, Nadja Sandra Katheder, James Kezos, Anna A. Kim, Seung K. Kim, Lutz Kockel, Sai Saroja Kolluru6,7,8, Nikolaos Konstantinides, Thomas B. Kornberg, Henry M. Krause, Andrew Thomas Labott, Meghan Laturney, Ruth Lehmann, Sarah Leinwand, Jure Leskovec, Hongjie Li1, Jiefu Li1, Joshua Shing Shun Li, Kai Li, Ke Li, Liying Li, Tun Li, Maria Litovchenko, Han-Hsuan Liu, Yifang Liu, Tzu-Chiao Lu, Liqun Luo, Sharvani Mahadevaraju, Jonathan Manning, Anjeli Mase, Mikaela Matera-Vatnick, Neuza Reis Matias, Erika L. Matunis, Caitlin E. McDonough-Goldstein, Aaron McGeever, Alex D. McLachlan, Colleen N. McLaughlin, Paola Moreno-Roman, Norma Neff, Megan Neville, Sang Ngo, Tanja Nielsen, Todd G. Nystul, Caitlin E. O’Brien, Lucy Erin O’Brien, Brian Oliver, David Osumi-Sutherland, Mehmet Neset Özel, Soumitra Pal, Irene Papatheodorou, Norbert Perrimon, Maja Petkovic, Clare Pilgrim68, Angela Oliveira Pisco, Teresa M. Przytycka, Stephen R. Quake, Carolina Reisenman, Carlos Ribeiro, Katja Rust, Wouter Saelens, Erin Nicole Sanders, Gilberto dos Santos, Frank Schnorrer, Kristin Scott, Aparna Sherlekar, Jiwon Shim, Philip Shiu, David Sims, Rene V. Sit, Maija Slaidina, Harold E. Smith, Katina Spanier, Gabriella Sterne, Yu-Han Su, Daniel Sutton, Marco Tamayo, Michelle Tan, Ibrahim Tastekin, Sudhir Gopal Tattikota, Christoph Treiber, David Vacek, Georg Vogler, Scott Waddell, Maxime De Waegeneer, Wanpeng Wang, Helen White-Cooper, Rachel I. Wilson, Mariana F. Wolfner, Yiu-Cheung E. Wong, Anthony Xie, Qijing Xie, Jun Xu, Shinya Yamamoto, Jia Yan, Zepeng Yao, Kazuki Yodaformat , Ruijun Zhu, Robert P. Zinzen, For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula Drosophilae, that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types. We provide an in-depth analysis of cell type–related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types between tissues, such as blood and muscle cells, reveals rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the Drosophila community and serves as a reference to study genetic perturbations and disease models at single-cell resolution., The sequencing was supported by the Chan Zuckerberg Biohub (S.R.Q.), Genentech Inc. (H.J.), National Institutes of Health intramural 1ZIADK015600 (B.O.), national funds through the FCT in the framework of the financing of the Norma Transitória DL 57/2016 (Z.C.-S.), Wu Tsai Neurosciences Institute at Stanford (S.R.Q. and L.L.), and the Howard Hughes Medical Institute and a National Institutes of Health grant (L.L.). Computational work was supported by the KU Leuven and the Flemish Supercomputer Center (VSC) (S.A.) and EPFL (B.D.). FCA Consortium funding is provided in the supplementary materials.