Search

Objective The tourism rail project at a scenic area is a single-line route. To enhance tourists transportation efficiency, it is required to arrange two sets of single-opening curved track turnout symmetrically within the existing curved sections. Therefore, it is necessary to design a turnout for same-side curved track to solve this practical engineering problem. Method Initially, the design requirements for the turnout are investigated, and the basic alignment of the same-side curved track turnout is determined. Various turnout types are compared, considering factors such as turnout action time, support form, and landscape design requirements. Design principles and key points of alignment are proposed, and parameters such as kinetic energy loss, unbalanced centrifugal acceleration, and unbalanced centrifugal acceleration increment are evaluated. The alignment and scheme designs are completed by considering factors like the alignment of the existing line, the installation space and method of turnout to meet the requirements of performance indicators and specific design needs of the turnout. Finite element analysis is conducted on the main components of the turnout based on factors including train load. Result & Conclusion The recommended turnout type is a single-crossing replacement beam type same-side curved turnout, which offers advantages such as simple and reliable structure, and high technical-economic efficiency, meeting the special requirements for turnout arrangement.

MicroRNA-940 (miR-940) has been extensively studied in the pathogenesis of numerous types of human cancer; however, the expression pattern, roles and molecular mechanisms underlying the regulatory actions of miR-940 in glioma remain unknown. The present study aimed to further investigate miR-940 by studying its expression, roles and mechanisms of action in glioma. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect miR-940 expression in glioma tissues and cell lines. The regulatory effects of miR-940 in glioma cell proliferation and invasion were determined using MTT and cell invasion assays. Bioinformatics analyses was performed to identify the potential target of miR-940, which was further confirmed by luciferase reporter assay, RT-qPCR and western blot analysis. In the present study, significantly increased miR-940 expression levels were observed in glioma tissues and cell lines compared with normal brain tissues and normal human astrocytes, respectively. Decreased miR-940 expression levels attenuated glioma cell proliferation and invasion in vitro. Kruppel-like factor 9 (KLF9) was predicted as a potential target of miR-940. Further assays demonstrated that miR-940 negatively regulated KLF9 expression in glioma cells by directly targeting the 3′-untranslated regions of KLF9. Additionally, KLF9 expression was downregulated in glioma tissues and was inversely correlated with miR-940. Furthermore, KLF9 knockdown was able to rescue the effects of miR-940 on glioma cell proliferation and invasion. The results of the present study suggest that miR-940 may function as an oncogene in glioma by targeting KLF9 and may be a considered a therapeutic target for the treatment of gliomas. [ABSTRACT FROM AUTHOR]

TMEM173 is a pattern recognition receptor detecting cytoplasmic nucleic acids and transmits cGAS related signals that activate host innate immune responses. It has also been found to be involved in tumor immunity and tumorigenesis. In this study, we first identified that the FKBP4/NR3C1 axis was a novel negative regulator of TMEM173 in human breast cancer (BC) cells. The effect of FKBP4 appeared to be at the transcriptional level of TMEM173, because it could suppress the promoter activity of TMEM173, thereby affecting TMEM173 at mRNA and protein levels. Past studies, our bioinformatics analysis, and in vitro experiments further implied that FKBP4 regulated TMEM173 via regulating nuclear translocation of NR3C1. We then demonstrated that the FKBP4/NR3C1/TMEM173 signaling pathway could regulate autophagy and proliferation of BC cells as well as dendritic cell (DC) abundance through exosome release. Our study found an unprecedented strategy used by BC to escape from TMEM173 mediated tumor suppression. Identification of the FKBP4/NR3C1 axis as a novel TMEM173 regulator would provide insights for novel anti-tumor strategy against BC among tumor microenvironment., Competing Interests: The authors declare that they have no competing interests., (© 2022 The Author(s).)