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Apple-picking robot can promote the development of smart agriculture, and accurate object recognition in complex natural environments using deep learning algorithms is critical. However, research has shown that changes in illumination and object occlusion remain significant challenges for recognition. In order to improve the accuracy of apple apple-picking robot's identification and positioning of apples in natural environment, a method using YOLOv5 (You Only Look Once, YOLO) combined with fast-guided filter is proposed. By introducing a fast-guided filtering module, the ability to extract image features is improved, and the problem of inaccurate occlusion targets and edge detection is solved; K -means clustering algorithm is introduced in improving YOLOv5, which can realize automatic adjustment of image size and step size; BiFPN structure is introduced in Neck network to add weighted feature fusion to highlight the detailed features. The results show that the algorithm proposed in this paper can well remove noise information such as occlusion edge blurring in apple images in a natural light environment. In the real orchard environment, the apple recognition accuracy rate reached 97.8%, the recall rate was 97.3% and the recognition rate was about 26.84 fps. The results show that this research based on YOLOv5 and fast-guided filtering can realize fast and accurate identification of apple fruits in natural environment, and meet the practical application requirements of real-time target detection. [ABSTRACT FROM AUTHOR]