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Background: The α-klotho (αKl) is widely accepted as an anti-aging and anti-inflammatory protein. However, it is rarely reported on the function and mechanism of αKl in the overall population (including healthy people and those with history of chronic disease). The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) are established as predictors of systemic inflammation. This study aims to investigate the relationship between NLR, PLR, and αKl levels in the overall population. Methods: Data from 10,124 adults aged 40 years old and above, collected from NHANES 2007–2016, were analyzed. Associations between NLR, PLR, and αKl levels were assessed by weighted multivariate linear regression analyses, adjusting for potential confounders. Subgroup analysis was conducted by gender, age, diabetes, cardiovascular disease, and chronic kidney disease. Results: Weighted linear regression models showed that a significant negative correlation was observed between both NLR and PLR with αKl levels. Subgroup analysis revealed that the negative correlation between NLR and serum αKl levels was not significant in individuals aged 40–59 years and males, while this relationship remained stable across most other subgroups. The negative correlation between PLR and serum αKl levels was consistent across most subgroups but not significant in individuals with cardiovascular disease. Conclusion: Our study revealed a significant negative relationship between inflammatory markers (NLR and PLR) and serum αKl levels, suggesting systemic inflammation may be linked to reduced αKl expression. Subgroup analyses showed that the relationship varies across different demographic and health-related factors. We provided insight into the significance of managing inflammation and preserving αKl levels.

Abstract The integration of electronic reconnaissance platforms and payloads in the electromagnetic and spatial domains of cognition is necessary for the successful execution of cognitive electronic reconnaissance missions. However, in partially observable environments, platforms and payloads interact in separate operational domains, making it a challenge for the model. The authors create a theoretical model of cognitive electronic reconnaissance, present an analysis method of model elements and derives adequate requirements for optimal radiation source sorting or recognition. Then, a 3D cognitive reconnaissance simulator ‘Scouter’ to simulate the dynamic interaction process of space electronic reconnaissance missions is developed and a deep reinforcement learning framework to optimise the electronic reconnaissance strategies of Unmanned Aerial Vehicle (UAV) is designed. For the first time, a method for judging reconnaissance area coverage using a behavioural angle is proposed. This method enables a deep reinforcement learning‐based UAV with better reconnaissance behaviours and to realise the stable targeting of the target by the receiving antenna beam during reconnaissance. Moreover, the fast convergence of UAV agent training is ensured through the normalisation of partially observable status and reward shaping. Verified by adequate numerical simulations, a trained UAV can complete a 100 reconnaissance cycles mission with a completion rate of 91.9%. Even after the reconnaissance cycles are prolonged to 600, the mission completion rate can still be maintained at 78.5%. The most satisfying aspect is that the UAV comprehends the idea of reconnaissance and behaves in a way that is typical of it, such as by reciprocating or circling the edge of the radar's detecting power.

KDM5B is essential for early embryo development, which is under the control of maternal factors in oocytes. Granulosa cells (GCs) play a critical role during oocyte mature. However, the role of KDM5B in GCs remains to be elucidated. In the current study, we found that KDM5B expressed highly in the ovaries and located in goat GCs. Using an RNA sequence, we identified 1353 differentially expressed genes in the KDM5B knockdown GCs, which were mainly enriched in cell cycle, cell division, DNA replication and the cellular oxidative phosphorylation regulation pathway. Moreover, we reported a decrease in the percentage of proliferated cells but an increase in the percentage of apoptotic cells in the KDM5B knockdown GCs. In addition, in the KDM5B knockdown GCs, the percentage of GCs blocked at the S phase was increased compared to the NC group, suggesting a critical role of KDM5B in the cell cycle. Moreover, in the KDM5B knockdown GCs, the reactive oxygen species level, the mitochondrial depolarization ratio, and the expression of intracellular phosphorylated histone H2AX (γH2AX) increased, suggesting that knockdown of KDM5B leads to DNA damage, primarily in the form of DNA double-strand breaks (DSBs). Interestingly, we found a down-regulation of MTF1 in the KDM5B knockdown GCs, and the level of cell proliferation, as well as the cell cycle block in the S phase, was improved. In contrast, in the group with both KDM5B knockdown and MTF1 overexpression, the level of ROS, the expression of γH2AX and the number of DNA DSB sites decreased. Taken together, our results suggest that KDM5B inhibits DNA damage and promotes the cell cycle in GCs, which might occur through the up-regulation of MTF1.

Pushed by social demands, China has continuously developed its low-medium speed maglev transportation systems in recent decades. However, with the increase of the running mileage of the train, high frequency vibration of suspension frame gradually appears. In this paper, a method to analyze the high frequency vibration of suspension frame by detecting the current of traction motor is proposed. Field measurements of suspension frame vibration and traction motor current of low-medium maglev train were conducted on a maglev line that has been commercially put into operation. The test results show that the speed of the measured maglev train was closely correlated with vibration accelerations of the suspension frame in three directions, but as the speed increased, the influence it imposed on the suspension frame vibration waned. The dominant frequency of traction motor resultant current was consistent with the dominant frequency of the suspension frame vibration acceleration. The switching frequency of traction inverter was the main cause of high frequency vibration of suspension frame.

The J-TEXT capability is enhanced compared to two years ago with several upgrades of its diagnostics and the increase of electron cyclotron resonance heating (ECRH) power to 1 MW. With the application of electron cyclotron wave (ECW), the ECW assisted plasma startup is achieved; the tearing mode is suppressed; the toroidal injection of 300 kW ECW drives around 24 kA current; fast electrons are generated with toroidal injected ECW and the runaway current conversion efficiency increases with ECRH power. The mode coupling between 2/1 and 3/1 modes are extensively studied. The coupled 2/1 and 3/1 modes usually lead to major disruption. Their coupling can be either suppressed or avoided by external resonant magnetic perturbation fields and hence avoids the major disruption. It is also found that the 2/1 threshold of external field is significantly reduced by a pre-excited 3/1 mode, which can be either a locked island or an external kink mode. The disruption control is studied by developing prediction methods capable of cross tokamak application and by new mitigation methods, such as the biased electrode or electromagnetic pellet injector. The high-density operation and related disruptions are studied from various aspects. Approaching the density limit, the collapse of the edge shear layer is observed and such collapse can be prevented by applying edge biasing, leading to an increased density limit. The density limit is also observed to increase, if the plasma is operated in the poloidal divertor configuration or the plasma purity is increased by increasing the pre-filled gas pressure or ECRH power during the start-up phase.

Weighted fractional Fourier transform encrypted chaotic direct sequence spread spectrum (WFRFT-CD3S) signal is aperiodic and complex Gaussian distributed. The aperiodicity and complex Gaussian distribution are conducive to the covert transmission of information but also make it difficult to acquire the WFRFT-CD3S signal. An acquisition method implemented in two steps is proposed to acquire the Doppler-shifted WFRFT-CD3S signal. First, a bidirectional correlation search method is proposed to align the received signal with the local spreading sequence in the time domain. The correlation peak attenuation caused by the Doppler frequency shift is eliminated by differential. Second, the accumulated phase angle caused by the Doppler frequency shift is obtained by the differential correlation value between the aligned received signal and the local spreading sequence. Then, the Doppler frequency shift is estimated by the accumulated phase angle. The detection probability, the false alarm probability, and the root mean square error (RMSE) of the Doppler shift estimation are analyzed theoretically and simulated. The theoretical results are verified by simulation. Theoretical results and simulations show that the detection probability is higher than 0.999, the false alarm probability is less than 0.001, and the RMSE of Doppler shift estimation is lower than 63 Hz when the signal-to-noise ratio is higher than −7.3 dB.

In this paper, we introduce the property (h) on Banach lattices and present its characterization in terms of disjoint sequences. Then, an example is given to show that an order-to-norm continuous operator may not be σ-order continuous. Suppose T:E→F is an order-bounded operator from Dedekind σ-complete Banach lattice E into Dedekind complete Banach lattice F. We prove that T is σ-order-to-norm continuous if and only if T is both order weakly compact and σ-order continuous. In addition, if E can be represented as an ideal of L0(μ), where (Ω,Σ,μ) is a σ-finite measure space, then T is σ-order-to-norm continuous if and only if T is order-to-norm continuous. As applications, we extend Wickstead’s results on the order continuity of norms on E and E′.

Citrus is one of the most important fruits in China. Miyagawa Satsuma, one kind of citrus, is a nutritious agricultural product with regional characteristics of Chongming Island. Near-infrared Spectroscopy (NIR) is a proper method for studying the quality of fruits, because it is low-cost, efficient, non-destructive, and repeatable. Therefore, the NIR technique is used to detect citrus's soluble solid content (SSC) in this study. After obtaining the original spectral data, the first 70% of them are divided into the training set and 30% into the test set. Then, the Random Frog algorithm is chosen to select characteristic wavelengths, which reduces the dimension of the data and the complexity of the model, and accordingly makes the generalization of the classification model better. After comparing the performance of various classifiers (AdaBoost, KNN, LS-SVM, and Bayes) under different characteristic wavelength numbers, the AdaBoost classifier outperforms using 275 characteristic wavelengths for modeling eventually. The accuracy, precision, recall, and F1-score are 78.3%, 80.5%, 78.3%, and 0.780, respectively and the ROC (Receiver Operating Characteristic Curve, ROC curve) is close to the upper left corner, suggesting that the classification model is acceptable. The results demonstrate that it is feasible to use the NIR technique to estimate whether the citrus is sweet or not. Furthermore, it is beneficial for us to apply the obtained models for identifying the quality of citrus correctly. For fruit traders, the model helps them to determine the growth cycle of citrus more scientifically, improve the level of citrus cultivation and management and the final fruit quality, and thus increase the economic income of fruit traders.

Introduction and Objectives: Ubiquitin-specific proteases (USPs) act as proto-oncogenes or tumor suppressors in a wide variety of cancers. In this study, we intended to explore the role of USP1 in hepatocellular carcinoma (HCC). Materials and Methods: The clinical significance of USP1 in HCC was analyzed based on The Cancer Genome Atlas (TCGA) data and immunohistochemical staining. siRNAs and lentivirus were used to knock down and overexpress indicated genes, respectively. qRT-PCR and immunoblotting were performed to examine mRNA and protein expression, respectively. CCK8, colony formation and PI/Annexin V-APC staining were performed to examine cellular function. Immunoprecipitation, coomassie blue staining, mass spectrum and immunoblotting were conducted to evaluate the interaction between USP1 and c-kit. Results: USP1 was over-expressed in HCC patients. Patients with high expression of USP1 had shorter overall and disease free survival than those with low expression of USP1. Functional results showed that USP1 was critical for HCC cell growth and proliferation. Immunoprecipitation and immunoblotting results suggested that USP1 interacted with c-kit and promoted the stability of c-kit, which is an important target of lenvatinib in HCC. Knockdown of c-kit reversed the oncogenic function of USP1 on HCC cell growth. Lastly, USP1 upregulation conferred higher sensitivity of HCC cells to lenvatinib treatment. Conclusions: Our study demonstrated that USP1 acted as an oncogene in HCC. It also promoted lenvatinib efficacy by stabilizing c-kit.

The chaotic direct sequence spread spectrum (CD3S) is one of the distinguished techniques providing physical layer secrecy and low probability of intercept (LPI) in wireless communication. Due to the noise-like and aperiodic characteristics of chaotic signals, the synchronization of CD3S signals is a challenging issue. A synchronization approach for aperiodic CD3S signals based on bidirectional correlation search (BDCS) is proposed. Through a joint acquisition of forward frequency domain matched filtering (FDMF) and backward FDMF, the synchronization module aligns received CD3S signals with local chaotic sequences without aid of the pilot signal. The probability distribution of correlator output is derived with additive white Gaussian noise and narrowband jamming. The probability of detection, probability of false alarm, and bit error rate are analyzed mathematically. An approximate solution of the local optimal acquisition threshold coefficient is obtained. These theoretical analyses are verified by numerical simulations, the results of which demonstrate that the proposed synchronization approach enables the CD3S system to resist interception and adapt to the environment with low signal-to-noise and signal-to-jammer ratios.

As a novel type of mineral resource, gas hydrate has received a considerable amount of attention worldwide. This seismic detection method can detect abnormal phenomena such as the BSR, blank zones, velocity anomalies and polarity inversion of gas hydrate and become an important method of gas hydrate detection. The occurrence area of gas hydrate in the South China Sea is usually buried deep beneath the seabed. The current method cannot meet the needs of the shape and structure detection of gas hydrate deposits. With the support of the National Key R&D Program of China, some key technologies have led to developmental breakthroughs, such as ultra-high-energy plasma sources, small-group-interval high-resolution seismic streamers, and distributed three-dimensional seismic acquisition. The seismic profile obtained north of the South China Sea shows that the stratum penetration depth reaches nearly 1000 m at a depth of 1500 m, and the vertical resolution is better than 1.5 m. This system can serve the needs of high-resolution exploration of gas hydrate resources.

The triazole pesticide is an organic nitrogen-containing heterocyclic compound with a 1,2,3-Triazole ring. In order to develop a potential glucosamine-6-phosphate synthase (GlmS) inhibitor bactericide, 18 triazole-derivative compounds were synthesized efficiently. In addition, these compounds have not been reported in the literature. The structure was confirmed by high-resolution mass spectrometry (HRMS), 1H NMR and 13C NMR. The potential use of the most promising derivatives has been investigated by testing their antifungal activity and enzyme inhibitory activity, revealing inhibitory activities in the low micromolar range. Among them, the antifungal effects of compounds 1e, 1f, 1g, 2e, 2f, and 2g on Sclerotinia sclerotiorum were particularly significant, all of which were above 83%. These compounds will be further investigated as potential antifungal lead compounds. Their structure–activity relationships are discussed based on the effects of substituted phenyl groups on compounds.

Abstract The purpose of this paper is to introduce and study the general viscosity approximation methods for quasi-nonexpansive mappings in the setting of infinite-dimensional Hilbert spaces. Under suitable conditions, we prove that the sequences generated by the proposed new algorithm converge strongly to a fixed point of quasi-nonexpansive mappings in Hilbert spaces, which is also the unique solution of some variational inequality. Then this result is used to study the split equality fixed point problems, the split equality common fixed point problems, the split equality null point problems, etc. Our results improve and generalize many results in the literature and they should have many applications in nonlinear science.

Concrete in which EPS (expanded polystyrene) particles partially or completely replace concrete aggregates is called EPS concrete. Compared to traditional concrete, EPS concrete has a controllable low density and good thermal-insulation performance, which make it promising for prospective applications. At present, research on EPS concrete mostly focuses on increasing its strength and EPS surface modifications. Few researchers have studied the influence of cementitious material strength and EPS-concrete density on the strength of EPS concrete. In this research, cement was used as the main material, and fly ash, silica fumes, and blast furnace slag were selected as admixtures. By changing the mixing proportions of the admixtures, the basic properties, such as the paste strength, change. Based on the mix proportions of the above different raw materials, EPS concrete with different density levels was prepared to explore the influence of the density of EPS concrete and the strength of cementitious materials on the strength of EPS concrete. The influence of the slurry strength on EPS-concrete strength was weaker than that of the density of EPS concrete. When the strength range of the cementitious materials is 35.7~70.5 MPa, the compressive strength range of 1000 kg/m3, 1200 kg/m3, and 1400 kg/m3 EPS concrete is 8.8~17.6 MPa, 11.4~18.0 MPa, and 15.7~26.6 MPa, respectively. Based on the experiments, the fitting equation to determine the EPS-concrete strength–EPS-concrete density–cementitious material strength is z = 69.00087 + 0.0244x − 0.1746y − 0.00189x2 + 0.0000504706y2 + 0.00028401xy. Additionally, a strength-increasing design method for EPS concrete with different densities prepared by conventional Portland cement is clarified. This study can guide the preparation of EPS concrete.

With the increasing speed of high-speed trains, the service conditions of axle-box bearing system worsen, and meanwhile, the dynamic performance of the axle-box bearing directly affects the operational safety. However, the dynamic interactions of the axle-box bearing in the traditional vehicle-track system are often ignored. In this paper, a vehicle-track coupling dynamic model considering axle-box bearing has been built, and the effectiveness of the model is proved by field tests. Dynamic performance of the axle-box bearing has been analyzed and discussed through numerical simulations under different working conditions. Comparing the roller-raceway contact load characteristics under different working conditions, results show that the peak values of roller-outer raceway contact load with wheel-polygonal excitation are basically the same with those without wheel-rail excitation. However, most of the peak values of roller-outer raceway contact force under track irregularity and comprehensive excitation conditions are far greater than those under wheel-polygonal excitation and no wheel-rail excitation conditions, which indicates that the impact of track irregularity on the contact load is dominant.

Several recent papers investigated unbounded convergences in Banach lattices. The focus of this paper is to apply the results of unbounded convergence to the classical Banach lattice theory from a new perspective. Combining all unbounded convergences, including unbounded order (norm, absolute weak, absolute weak*) convergence, we characterize L-weakly compact sets, L-weakly compact operators and M-weakly compact operators on Banach lattices. For applications, we introduce so-called statistical-unbounded convergence and use these convergences to describe KB-spaces and reflexive Banach lattices.

Recently, continuous functionals for unbounded order (norm, weak and weak*) in Banach lattices were studied. In this paper, we study the continuous operators with respect to unbounded convergences. We first investigate the approximation property of continuous operators for unbounded convergence. Then we show some characterizations of the continuity of the continuous operators for uo, un, uaw and uaw*-convergence. Based on these results, we discuss the order-weakly compact operators on Banach lattices.

Carbon is the crucial source of energy during aerobic composting. There are few studies that explore carbon preservation by inoculation with microbial agents during goat manure composting. Hence, this study inoculated three proportions of microbial agents to investigate the preservation of carbon during goat manure composting. The microbial inoculums were composed of Bacillus subtilis, Bacillus licheniformis, Trichoderma viride, Aspergillus niger, and yeast, and the proportions were B1 treatment (1:1:1:1:2), B2 treatment (2:2:1:1:2), and B3 treatment (3:3:1:1:2). The results showed that the contents of total organic carbon were enriched by 12.21%, 4.87%, and 1.90% in B1 treatment, B2 treatment, and B3 treatment, respectively. The total organic carbon contents of B1 treatment, B2 treatment, and B3 treatment were 402.00 ± 2.65, 366.33 ± 1.53, and 378.33 ± 2.08 g/kg, respectively. B1 treatment significantly increased the content of total organic carbon compared with the other two treatments (p < 0.05). Moreover, the ratio of 1:1:1:1:2 significantly reduced the moisture content, pH value, EC value, hemicellulose, and lignin contents (p < 0.05), and significantly increased the GI value and the content of humic acid carbon (p < 0.05). Consequently, the preservation of carbon might be a result not only of the enrichment of the humic acid carbon and the decomposition of hemicellulose and lignin, but also the increased OTU amount and Lactobacillus abundance. This result provided a ratio of microbial agents to preserve the carbon during goat manure aerobic composting.

According to the basic principle of the hydraulic anti-kink system and flow continuity equation, this paper takes the low-floor tram as the research object and the four vehicles as the research carrier. Based on the correlation parameters between the vehicle subsystem and the hydraulic subsystem, a co-simulation platform of a low-floor tram with hydraulic an anti-kink system is built. The co-simulation results show that the anti-kink system can well maintain the relative yaw angle consistency between the vehicle body and bogie. The anti-kink system restrains the maximum yaw angle and excessive lateral displacement of the vehicle body effectively. The consistency between the experiment results and the simulation results shows the accuracy of the model. The co-simulation model of the low-floor tram with hydraulic anti-kink system can be used to research the dynamic performance when it passes through curve line.

Root restriction cultivation leads to early maturation and quality improvement, especially in the anthocyanin content in grapevine. However, the molecular mechanisms that underlie these changes have not been thoroughly elucidated. In this study, four small RNA libraries were constructed, which included the green soft stage (GS) and ripe stage (RS) of ‘Muscat’ (Vitis vinifera L.) grape berries that were grown under root restriction (RR) and in traditional cultivation (no root restriction, CK). A total of 162 known miRNAs and 14 putative novel miRNAs were detected from the four small RNA libraries by high-throughput sequencing. An analysis of differentially expressed miRNAs (DEMs) revealed that 13 miRNAs exhibited significant differences in expression between RR and CK at the GS and RS stages, respectively. For different developmental stages of fruit, 23 and 34 miRNAs showed expression differences between the GS and RS stages in RR and CK, respectively. The expression patterns of the eight DEMs and their targets were verified by qRT-PCR, and the expression profiles of target genes were confirmed to be complementary to the corresponding miRNAs in RR and CK. The function of Vvi-miR828, which showed the down regulated expression in the RS stage under root restriction, was identified by gene transformation in Arabidopsis. The anthocyanin content significantly decreased in transgenic lines, which indicates the regulatory capacity of Vvi-miR828 in fruit coloration. The miRNA expression pattern comparison between RR and CK might provide a means of unraveling the miRNA-mediated molecular process regulating grape berry development under root restricted cultivation.

Triazole pesticides are organic nitrogen-containing heterocyclic compounds, which contain 1,2,3-triazole ring. In order to develop potential glucosamine-6-phosphate synthase (GlmS) inhibitor fungicides, forty compounds of triazole derivatives were synthesized in an efficient way, thirty nine of them were new compounds. The structures of all the compounds were confirmed by high resolution mass spectrometer (HRMS), 1H-NMR and 13C-NMR. The fungicidal activities results based on means of mycelium growth rate method indicated that some of the compounds exhibited good fungicidal activities against P. CapasiciLeonian, Sclerotinia sclerotiorum (Lib.) de Bary, Pyricularia oryzae Cav. and Fusarium oxysporum Schl. F.sp. vasinfectum (Atk.) Snyd. & Hans. at the concentration of 50 µg/mL, especially the inhibitory rates of compounds 1-d and 1-f were over 80%. At the same time, the preliminary studies based on the Elson-Morgan method indicated that the compounds exhibited some inhibitory activity toward glucosamine-6-phosphate synthase (GlmS). These compounds will be further studied as potential antifungal lead compounds. The structure-activity relationships (SAR) were discussed in terms of the effects of the substituents on both the benzene and the sugar ring.

A new type of blue emitter, N2-Indolyl-1,2,3-triazoles (NITs), with the λmax ranging from 420–480 nm and the Stokes shift from 89–143 nm, were synthesized through the coupling reaction of indoles with triazole derivatives. The influence of different substitution patterns on the optical properties (efficiency, excitation, and emission wavelengths) of the NITs was investigated. In addition, one palladium complex were synthesized by using NITs as the ligands, which, however, exhibited no fluorescent activity, but did show the enhanced co-planarity. Lastly, two bio-active molecule derivatives were explored for the potential use of these novel dyes in related chemical and biological applications.

The poor spectral stability of laser-induced breakdown spectroscopy (LIBS) seriously affects its analytical performance, which is a key obstacle to its further development. To overcome this challenge, an improved spectral standardization method based on plasma image-spectrum fusion (ISS-PISF) was proposed in this study. This method, for the first time, considers and quantifies the influence of plasma morphology on spectral intensity based on the line-integrated intensity formula of LIBS spectra. It recognizes that the spectral fluctuations mainly stem from variations in total number density, plasma temperature, electron number density, and plasma morphology. Therefore, ISS-PISF innovatively utilizes easily accessible features from plasma images and spectra to eliminate the influence of these four plasma parameters, thereby improving the spectral stability and analytical performance of LIBS. To validate the effectiveness of this method, the spectra of aluminum alloy samples obtained under complex detection conditions simulated by varying laser energy and defocusing amount were analyzed. After correction by ISS-PISF, the R2 for Mg I 516.73 nm, Mn II 294.92 nm, and Si I 288.16 nm improved to 0.990, 0.976, and 0.961, and the average RMSE of the validation set decreased by 46.154%, while the average STD of the validation set decreased by 37.405%. These experimental results indicate that this study provides a simple, effective, and physically supported spectral standardization method, which contributes to the further promotion and application of LIBS. [ABSTRACT FROM AUTHOR]