1. Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay.
- Author
-
Villa-Bellosta R and Hamczyk MR
- Subjects
- Animals, Aorta drug effects, Aorta metabolism, Aorta pathology, Aortic Diseases chemically induced, Aortic Diseases metabolism, Calcium Phosphates toxicity, Cells, Cultured, Collagenases metabolism, Crystallization, Durapatite metabolism, Mice, Muscle, Smooth, Vascular drug effects, Muscle, Smooth, Vascular metabolism, Myocytes, Smooth Muscle drug effects, Myocytes, Smooth Muscle metabolism, Vascular Calcification chemically induced, Vascular Calcification metabolism, Aortic Diseases pathology, Cell Separation methods, Muscle, Smooth, Vascular pathology, Myocytes, Smooth Muscle pathology, Primary Cell Culture, Vascular Calcification pathology
- Abstract
Elevated serum phosphorus is a major risk factor for vascular calcification, which is characterized by the presence of calcium phosphate deposits, mainly hydroxyapatite crystals. In vitro studies of phosphate-induced calcification show that vascular smooth muscle cells undergo calcification with features similar to those observed in pathological vascular calcification in vivo, including the presence of hydroxyapatite crystals. Here, we describe the double-collagenase digestion method for isolating vascular smooth muscle cells from aorta, and a method for inducing calcification in vitro using high phosphate concentration.
- Published
- 2015
- Full Text
- View/download PDF