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Background During the last few decades, patients worldwide have been interested in using alternative medicine in treating diseases to avoid the increased side effects of chemical medications. Green coffee is unroasted coffee seeds that have higher amounts of chlorogenic acid compared to roasted coffee. Green coffee was successfully used to protect against obesity, Alzheimer disease, high blood pressure and bacterial infection. Methods This study aimed to investigate the probable protective activity of the green coffee methanolic extract, silymarin and their combination on CCl4-induced liver toxicity in male rats. Thirty Sprague – Dawley male albino rats were divided into 5 groups; control negative (G1) just got the vehicle (olive oil) and the other four groups received CCl4 dissolved in olive oil through an intraperitoneal injection and were divided into untreated control positive group (G2), the third group (G3) was treated with green coffee methanolic extract, the fourth group (G4) was treated with silymarin, and the fifth group (G5) was treated with a combination of green coffee methanolic extract and silymarin. Results In the positive control group treated with CCl4 (G2), the CCl4-induced toxicity increased lipid peroxidation, IL-6, kidney function parameters, liver function enzymes, total cholesterol, triglycerides and low-density lipoproteins, and decreased irisin, antioxidants, CYP450 and high-density lipoprotein levels. Hepatic tissues were also injured. However, treating the injured rats in G3, G4 and G5 significantly improved the altered parameters and hepatic tissues. Conclusions Green coffee methanolic extract, silymarin, and their combination succeeded in protecting the male rats against CCl4 hepatotoxicity due to their antioxidant activity. Effect of green coffee methanolic extract mixed with silymarin in G5 was more efficient than that of green coffee methanolic extract in G3 or silymarin in G4.

Introduction: Diabetic nephropathy (DN) is one of most prevalent diabetic complication. It remains unclear whether the anti-inflammatory Hydroxytyrosol (HT) has beneficial effects on biogenesis of diabetic renal changes. So, we aimed to find out the relationship between inflammation, apoptosis, oxidative stress and the progression of DN. Also, try to assess the possible effects of HT on diabetic renal tissue. Materials and Methods: Rats were divided into: non-diabetic rats (group I) and rats with induced type II diabetes that were subdivided into: group II non-treated DM, group III treated with HT, group IV treated with glyclazide and group V treated with combined treatment. Treatments were supplied for eight weeks. Results: Administration of HT alone or with GLY significantly lowered blood glucose levels and ameliorated kidney hypertrophy index together with improving renal dysfunction parameters including creatinine in serum and urine, blood urea nitrogen, serum and urinary albumin, together with the tissue oxidative markers and inflammatory cytokines activities compared to diabetic group. These effects of HT were also reflected on histologic evaluation and Nrf2- Keap1 system expression. Conclusion: This study discovers the renoprotective effect of HT in diabetic rats. Hence, this study recommended an addition of antioxidants like HT to the management of diabetes.