Search

The cell cycle is modulated by ubiquitin ligases, including CRL4, which facilitate degradation of the chromatin-bound substrates involved in DNA replication and chromosome segregation. One of the members of the CRL4 complex, RepID (DCAF14/PHIP), recognizes kinetochore-localizing BUB3, known as the CRL4 substrate, and recruits CRL4 to the chromatin/chromosome using the WD40 domain. Here, we show that the RepID WD40 domain provides different platforms to CRL4 and BUB3. Deletion of the H-box or exon 8 located in the RepID WD40 domain compromises the interaction between RepID and CRL4, whereas BUB3 interacts with the exon 1–2 region. Moreover, deletion mutants of other exons in the WD40 domain lost chromatin binding affinity. Structure prediction revealed that the RepID WD40 domain has two beta-propeller folds, linked by loops, which are possibly crucial for chromatin binding. These findings provide mechanistic insights into the space occupancy of the RepID WD40 domain to form a complex with CRL4, BUB3, or chromatin. • RepID WD40 domain is crucial for binding to chromatin and proteins. • WD40 domain of RepID forms a two-β propeller configuration connected by a loop. • RepID WD40 domain provides different platforms for proteins and chromatin. [ABSTRACT FROM AUTHOR]

Non- Saccharomyces yeasts have been used for many years due to their technological potential, particularly as a "booster" of wine fruity aroma in mixed fermentations with Saccharomyces cerevisiae. Recently, a new application has emerged, bioprotection, which consists in colonizing the environment in the context of sulfite reduction in wines. The chemical and sensory impact of non- Saccharomyces yeast according to different modes of application in a context of fermentation without addition of SO 2 was evaluated through trial with Merlot N. (Vitis vinifera L.). An effective niche occupation by non- Saccharomyces yeasts was highlighted during the prefermentary stages by Quantitative-PCR and MALDI-TOF MS identification. Chemical analysis (GC-MS and GC MS/MS) of finish wine showed the significant impact of the dose applications, with bioprotection characterized by linear esters and sequential application by acetates of higher alcohol contents. Moreover, a separation according to the species used in bioprotection was revealed. Finally, using a panel trained, the sensory analysis confirmed that the use of non- Saccharomyces yeast was a fruity booster in sequential inoculation and, to a less extent, when used as bioprotection. This study shows for the first time that the use of non- Saccharomyces yeast as a bioprotection has a significant impact on the aromatic profile of wines. • Occupying the niche by T. delbrueckii as a bioprotection, unlike S. cerevisiae. • The dosage of non- Saccharomyces significantly impact the chemical composition of red wine. • Impact of bioprotection on the chemical and aromatic profile red wine in low SO 2. [ABSTRACT FROM AUTHOR]

Cullin-RING ubiquitin E3 ligase (CRLs) composed of four components including cullin scaffolds, adaptors, substrate receptors, and RING proteins mediates the ubiquitination of approximately 20% of cellular proteins that are involved in numerous biological processes. While CRLs deregulation contributes to the pathogenesis of many diseases, including cancer, how CRLs deregulation occurs is yet to be fully investigated. Here, we demonstrate that components of CRL3 and its transcriptional regulators are possible prognosis marker of neuroendocrine (NE) cancer. Analysis of Cancer Cell Line Encyclopedia (CCLE) through the CellMinerCDB portal revealed that expression of CRL3 scaffold Cullin 3 (CUL3) highly correlates with NE signature, and CUL3 silencing inhibited NE cancer proliferation. Moreover, subset of 151 BTB (Bric-a-brac, Tramtrack, Broad complex) domain-containing proteins that have dual roles as substrate receptors and adaptor subunits in CRL3, as well as the expression of transcription factors (TFs) that control the transcription of BTB genes were upregulated in NE cancer. Analysis using published ChIP-sequencing data in small cell lung cancer (SCLC), including NE or non-NE SCLC verified that gene promoter of candidates which show high correlation with NE signature enriched H3K27Ac. These observations suggest that CRL3 is a master regulator of NE cancer and knowledge of specifically regulated CRL3 genes in NE cancer may accelerate new therapeutic approaches. • Cullin 3 is highly expressed in neuroendocrine cancer. • Subgroups of BTBs are highly expressed in neuroendocrine cancer. • Multiple modules in the transcription factors-BTBs-CRL3 axis are promising novel biomarkers in neuroendocrine cancer. [ABSTRACT FROM AUTHOR]

Predicting the vibration response of an elastic structure excited by a turbulent flow is of interest for the civil and military transportation sector. The models proposed in the literature are generally based on the assumption that the turbulent boundary layer (noted TBL in the following) exciting the structure is spatially homogeneous. However, this assumption is not always fulfilled in practice, in particular when the excited area is close to the starting point of the TBL or with curved structures. To overcome this issue, this work proposes to extend two approaches generally used for dealing with homogeneous TBL, namely the spatial and the wavenumber approaches. The extension of the spatial approach to non-homogeneous excitation is relatively straightforward and gives us a reference, however with costly computation. On the contrary, extending the wavenumber approach requires more developments and assumptions that have led the authors to develop a sub-area decomposition technique (SDT). It consists in partitioning the excited area in several sub-areas, assuming a homogeneous TBL pressure field on each sub-area and neglecting certain interactions between the sub-areas. A criterion on the sub-area size as a function of the minimum wavelength of the wall pressure field is proposed on the basis of the numerical calculations. A test case on a plate with varying thickness and excited by a growing TBL allows us to highlight: (1) the interest of the SDT compared to a classical calculation considering a homogeneous TBL; (2) the efficiency of the SDT in terms of computing time compared to the spatial approach. • Vibration of flexible panels excited by non-homogeneous turbulent boundary layer. • Spatial and wavenumber formulations are investigated for numerical modeling. • Extensions proposed for taking the spatial variations of the excitation into account. • Vibrations of a plate beneath a growing turbulent boundary layer are analyzed. • The physical phenomena induced by the spatially varying of the excitation are highlighted. [ABSTRACT FROM AUTHOR]

Maintaining wine oxidative stability and reducing SO 2 addition remains a challenge in white winemaking. This study aimed to evaluate the antioxidant capacity of two yeast derivatives (YDs) with a specific composition: one was rich in reducing compounds, including glutathione (YD R), and the other was rich in lipid compounds (YD L). Both YDs were evaluated for their antiradical activity in model wine solution (by the DPPH method) and for their capacity to consume oxygen in model wine solution and in white wine. Antiradical activity in both matrixes and oxygen consumption rate in wine were higher for YD L than for YD R. However, the addition of YD R to wine limited the production of acetaldehyde and preserved glutathione content to a greater extent after wine oxygenation. The sensory analysis confirmed that both YDs, in particular YD L , limited the occurrence of oxidation off-odours when no SO 2 was added. These data suggest that the use of YDs could be effectively implemented in low-sulfite winemaking in order to improve the antioxidant protection of white wine. • The use of yeast derivatives (YDs) as antioxidants in white wine was evaluated. • Antioxidant activity were evaluated by DPPH and Oxygen Consumption Rate method. • The addition of YDs to white wine limited the production of acetaldehyde. • The addition of YDs to white wine permitted to preserve wine glutathione content. • YDs limited the color browning and oxidation off-odours in absence of SO 2 addition. [ABSTRACT FROM AUTHOR]