1. Antibacterial activity and action mode of crude bacteriocin C2-1 from Ligilactobacillus salivarius C2-1 against Listeria monocytogenes CICC 21633.
- Author
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Mu, Yulin, Zhang, Chengxiao, Jin, Chun-Zhi, Li, Taihua, Jin, Feng-Jie, Lee, Hyung-Gwan, and Jin, Long
- Subjects
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LISTERIA monocytogenes , *ANTIBACTERIAL agents , *WHOLE genome sequencing , *PEPSIN , *MEMBRANE permeability (Biology) , *CELL permeability , *CELL membranes - Abstract
The potential use of Ligilactobacillus salivarius -derived bacteriocin for the control of L. monocytogenes was investigated. The purpose of the study was to explore the antibacterial action mode of crude bacteriocin C2-1 from Ligilactobacillus salivarius C2-1 against Listeria monocytogenes CICC 21633. The inhibition zone diameter of crude bacteriocin C2-1 was 27.82 ± 0.3 mm against L. monocytogenes CICC 21633, and the minimum inhibitory concentration (MIC) of bacteriocin C2-1was 13.98 μg/mL. Bacteriocin C2-1 significantly inhibited the normal growth of L. monocytogenes CICC21633, is sensitive to proteinase K, trypsin, and pepsin, and has good thermal stability and acid tolerance. The antibacterial mode of bacteriocin C2-1 against L. monocytogenes CICC 21633 was discussed based on changes in cell membrane permeability, integrity, and morphological structure of L. monocytogenes CICC 21633 by measuring the conductivity, UV-absorbing substance content and the observation via scanning electron microscopy, respectively. These results demonstrated that bacteriocin C2-1 affected the cell membrane permeability and integrity of L. monocytogenes CICC 21633, resulting in intracellular material leakage. Furthermore, bacteriocin-producing genes were identified in the genome sequence using antiSMASH and BAGEL4 assays. Bacteriocin C2-1 might be a good biological food preservative to prevent L. monocytogenes infestation. • Bacteriocin C2-1 has antimicrobial activity against Listeria monocytogenes CICC 21633. • Bacteriocin C2-1 can strongly inhibit the normal growth of Listeria monocytogenes CICC 21633. • High heat stability, pH tolerance, and protease sensitivity are all characteristics of bacteriocin C2-1. • The antibacterial action mode of bacteriocin C2-1 was studied against Listeria monocytogenes CICC 21633. • Whole-genome sequencing analysis revealed the presence of bacteriocin biosynthetic gene clusters in Lb. salivarius C2-1. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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