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Multidimensional (coupled land use, soil properties, and topography) landscape effects on stream water nitrogen (N) are complex and scale-dependent. However, studies that identify critical buffer zones that explain large variations in riverine N, and estimate specific thresholds of multidimensional landscape patterns at the class level, result in a sudden changes in riverine N pollution, are still limited. Here, a new multidimensional landscape metric that combined land use, soil properties, and topography effects was applied to various riparian buffer zones and sub-watershed scales, and their relationships to riverine N levels were investigated. We used stream water ammonium−N, nitrate−N, and total−N concentrations datasets, from 2010 to 2017, in the nine subtropical sub-watersheds in China. The results of model selection and model averaging in ordinary least squares regressions, indicated that the riparian buffer zone with widths of 400 m, had more pronounced influence on water NH 4 –N and TN levels than at other scales. Within the 400 m buffer zone, the key landscape metrics for NH 4 –N, NO 3 –N and TN concentrations in stream water were different, and explained up to 43.35%–76.55% (adjusted R2) of the total variation in river N levels. When ENN_MN Class17 below 39–56 m, PD Class8 above 4.63–6.55 n/km2, PLAND Class27 above 23–29%, and CONTIG_MN Class42 below 0.35–0.37% within the 400 m buffer zone, riverine NH 4 –N and TN would be abruptly increased. This study provided practical ideas for regulation regarding landscape management linked to watershed structure, and identified reference thresholds for multidimensional landscape metrics, which should help reduce riverine N pollution in subtropical China. • Multidimensional landscape patterns affect riverine N at watershed outlets. • The 400 m riparian buffer zone has the strongest influence on riverine NH 4 –N and TN concentrations. • The ENN_MN Class17 , PD Class8 , PLAND Class27 , and CONTIG_MN Class42 are important metrics to characterize riverine N. • There exist change points in riverine N along a gradient of multidimensional landscape metrics. [ABSTRACT FROM AUTHOR]