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Study Objective: In this video, we describe a 5-step surgical technique that allows us to safely incise and aspirate the content of large ovarian cysts through a single-port laparoscopic incision. This allows performing laparoscopic oophorectomies instead of large xipho-pubic laparotomies.Design: A stepwise demonstration of the technique with narrated video footage.Setting: Ovarian masses, especially cysts, are common gynecologic conditions [1]. However, depending on their size, large adnexal cysts are usually managed with transverse or midline laparotomies [2]. This is to prevent cyst ruptures and abdominal contamination and ensure oncological safety of the procedure [3-5]. Different leak-proof aspiration techniques have been described in the literature allowing for safe large cyst aspiration and adnexectomy through a mini-laparotomy incision or via laparoscopy [2,3,6-10]. We describe a 5-step surgical technique allowing for closed aspiration of ovarian intracystic fluid and adnexectomy while respecting oncological safety.Interventions: Step 1: Perform diagnostic laparoscopy to rule out peritoneal carcinomatosis contraindicating this procedure and then after cyst exposition, thoroughly dry the cyst wall. Step 1 Bis: Cut the cuff of a sterile glove to prepare a square piece of membrane. Step 2: Place a protective gauze and then apply the surgical glue to the ovarian cyst wall followed by the glove/membrane application. Perform a purse suture through the glove/membrane and the ovarian wall superficially to ensure further adhesion and prevent ovarian fluid spillage. Step 3: Incise the ovarian wall, introduce the aspiration cannula and tighten the purse suture to aspirate the cystic fluid. Step 4: After aspiration is complete, tighten the suture and close the glove to guarantee a closed space and prevent abdominal contamination. Step 5: Perform laparoscopic oophorectomy or cystectomy. Safely remove the specimen in an endoscopic retrieval bag through the trocar incision.Conclusion: This technique allows safe laparoscopic large ovarian cysts resections while respecting oncologic safety and preventing intra-abdominal spillage and contamination. [ABSTRACT FROM AUTHOR]

• ENVI-met is validated to analyze the effect of albedo on courtyard microclimate. • Albedo of surfaces is found to have little effect on courtyard air temperature. • Albedo has an important effect on surface temperature, MRT and comfort. • High albedo of the walls reduces surface temperature but increases MRT. • Medium-range albedo is recommended to balance positive and negative effects. The Urban Heat Island (UHI) effect represents a threat to the well-being of cities. Cities must adapt to this phenomenon, prioritizing the improvement of outdoor environment quality. Urban materials have a great impact on outdoor environment quality, energy demand, and citizens' well-being. Based on the literature, it can be stated that changing the albedo of on-site materials (pavements, facades) is a relevant strategy. The environmental impact of reflective materials from buildings to urban microclimate has been widely discussed in the literature. However, few publications assess the role of albedo in inner courtyards. This work uses simulation results to evaluate the impact of different surface albedo on the thermal performance and comfort of a courtyard in Seville. To do so, the simulation tool ENVI-met, one of the most widely used for outdoor spaces, is validated through a comparison with monitoring results. In conclusion, high reflectance compromises user comfort up to 5 °C of PET despite the fact that the use of high albedo on surfaces reduces surface temperature up to 25 °C in comparison with low albedo as it accumulates less heat by reflecting more solar radiation. Some of the recommendations given include the use of medium albedo (around 0.4) on walls to balance positive and negative effects, and high albedo on the pavements (above 0.7). [ABSTRACT FROM AUTHOR]

• Cerato-platanin protein has been identified and isolated from P. salina. • Hydrophobin protein has been identified and isolated from T. pinophilus. • CPs and HFBs are extracted from a fermented culture medium with seaweed biomass. • Self-assemble of this extracted proteins were influenced by the polysaccharides' seaweed. The marine fungi Paradendryphiela salina and Talaromyces pinophilus degrade and assimilate complex substrates from plants and seaweed. Additionally, these fungi secrete surface-active proteins, identified as cerato-platanins and hydrophobins. These hydrophobic proteins have the ability to self-assemble forming amyloid-like aggregates and play an essential role in the growth and development of the filamentous fungi. It is the first time that one cerato-platanin (CP) is identified and isolated from P. salina (PsCP) and two Class I hydrophobins (HFBs) from T. pinophilus (TpHYD1 and TpHYD2). Furthermore, it is possible to extract cerato-platanins and hydrophobins using marine fungi that can feed on seaweed biomass, and through a submerged liquid fermentation process. The propensity to aggregate of these proteins has been analyzed using different techniques such as Thioflavin T fluorescence assay, Fourier-transform Infrared Spectroscopy, and Atomic Force Microscopy. Here, we show that the formation of aggregates of PsCP and TpHYD, was influenced by the carbon source from seaweed. This study highlighted the potential of these self-assembling proteins generated from a fermentation process with marine fungi and with promising properties such as conformational plasticity with extensive applications in biotechnology, pharmacy, nanotechnology, and biomedicine. [ABSTRACT FROM AUTHOR]