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• Endoscopic port surgery is a promising alternative for intracranial hemorrhage. • Precise location of basal ganglia hematoma is a crucial step for surgery. • A simple, low-cost navigation method using an Android smartphone. • The low-cost navigation is crucial for satisfactory evacuation of hematoma. Endoscopic port surgery is a promising alternative for the surgical treatment of intracerebral hypertensive basal ganglia hemorrhage (HBGH). The precise location of hematoma is a crucial step for surgery. The authors developed a simple, low-cost navigation method using an Android smartphone for the localization of HBGH. All patients' CT DICOM data were processed with an open-source software (3D Slicer). The volume of hematoma, angle, and length of trajectory were calculated automatically. A smartphone running the Android system and the Compass APP was used to help insert the inner introducer. An endoscopic port system was applied to create a working channel for neuro-endoscopic hematoma evacuation. There were 27 patients enrolled in this study (mean age 56). All patients underwent successful surgical evacuation of HBGH with neuroendoscopic evacuation. The mean time taken for the surgical plan was 4 min. The total operation time from skin incision to final suture was 82.6 min. Compared with standard neuronavigation, mean error of trajectory was 5.1 mm. The mean preoperative hematoma volume was 44.8 ml. The optimal trajectory angle averaged 39.5°and the length was 71 mm. Intraoperative blood loss was about 45 ml. Post-operative hematoma volume was 2.9 ml, and the average evacuation rate was 93.6%. One week after surgery, the mean GCS score was improved from 8.2 to 13.8 (p < 0.01). This simple, low-cost navigation method using 3D Slicer, an Android smartphone with the Compass APP, helps precisely insert the endoscopic working channel to the desired point, which is crucial for satisfactory evacuation of HBGH. [ABSTRACT FROM AUTHOR]

• Most of the cavernomas at the FoM conducted microscopic operations for the resection. • We performed the VE assisted ventriculoscopic operation through a single burr hole. • The VE provided a three-dimension and panorama view of the intraventricular lesions. Cavernomas at Foramen of Monroe (FoM) are rare cases among the intracranial cavernomas. Pure ventriculoscopic removal of cavernoma at FoM through a single burr hole is challenging and rarely reported. We herein introduced the virtual endoscopy (VE) assisted ventriculoscopic resection to treat the cavernomas at FoM. Two cases diagnosed with cavernomas at FoM, a 31-year-old male patient (case 1) and a 26-year-old male patient (case 2), were included. Both of them suffered from headache, nausea and vomiting. The pre-operative MRI revealed masses at the FoM. We reconstructed the VE on a free and open-source platform (3D Slicer) for the pre-surgical evaluation. And then ventriculoscopic operation through a single burr hole was made to remove the cavernomas at FoM. The VE displayed a 14×19×16 mm lesion in case 1 and an 18×20×29 mm lesion in case 2 and both cases revealed some attachment between the lesions and the periventricular tissue. The ventriculoscopic operations indicated by VE were performed to achieve total resection of the cavernomas without neurological deficit. Although the neuroendoscopic treatment to cavernoma at FoM through a single burr hole was rarely reported among the previous literatures, it was a quite effective and useful method in our cases. And the application of VE before ventriculoscopic operation could help to provide a three-dimensional and panorama view of the intraventricular lesions. [ABSTRACT FROM AUTHOR]

Although mixed land use is an important way to improve land-use efficiency and stimulate socioeconomic vitality, there are few systematic analyses of mixed land use in rural settlements. This paper proposes the connotation and measurements of mixed land use in rural settlements, considering both land-use diversity and compatibility. Case studies of Tangwei village (a tourist village), Guxia village (a commercial village), and Fulong village (an industrial village) in Dongguan City, Pearl River Delta, China, are chosen to analyze the characteristics of mixed land use in different types of rural settlements using field research, POI data capture, GIS, and remote sensing techniques. The results indicate that: (1) In 1978, 2005, and 2020, the degree of mixed land use in the rural settlement of Tangwei village was 0.68, 0.84, and 0.96, respectively, reflecting an increase of 41.17%; in Guxia village, it was 0.69, 0.86, and 0.84, respectively, growing and then declining, yielding a total increase of 21.73%; in Fulong village, it was 0.74, 0.82, and 0.89, respectively, reflecting an increase of 20.27%. (2) In 1978, 2005, and 2020, the land-use diversity of Tangwei village was 0.44, 0.94, and 0.92, respectively, representing a 109% improvement; land-use compatibility was 0.68, 0.72, and 0.96, respectively, with an increase of 41.17%. The land-use diversity of Guxia village was 0.46, 0.76, and 0.78, respectively, with an increase of 69.57%; land-use compatibility climbed by 24.64% from 0.69 to 1 to 0.84. The land-use diversity of Fulong village was 0.53, 0.83, and 0.88, respectively, which increased by 66.03%; land-use compatibility was 0.74, 0.81, and 0.89, respectively, which increased by 20.27%. (3) The mixed land use of Tangwei village is primarily influenced by policy support, resource endowment, and industrial foundation. The primary drivers of Guxia village are policy support, geographic location, and industrial foundation. Policy support, resource endowment, and industrial foundation are the most critical factors in Fulong village. Ultimately, land-use diversity and compatibility determine the mixed land use of rural settlements. This study offers suggestions for village planning, rural industrial development, and enhancing the living environment in China. • A conceptual framework of mixed land use in rural settlements was established. • The characteristics and driving forces of mixed land-use evolution in rural settlements were revealed. • Considering land-use diversity and compatibility improved the measurement of mixed land use. • Mixed land use of rural settlements can promote the coordinated improvement of villagers' living conditions and rural industrial development. [ABSTRACT FROM AUTHOR]

[Display omitted] • Assessing variants in non-canonical splicing sites is important during the process of exome sequencing-based primary hyperoxaluria diagnosis. • Capture sequencing-based copy number variant detection should be conducted in patients undergoing exome sequencing to diagnose suspected primary hyperoxaluria. • Exome sequencing will become a standard approach to diagnosing primary hyperoxaluria in pediatric urolithiasis patients. Next generation sequencing-based exome sequencing can be used to identify genetic abnormalities in patients believed to be suffering from primary hyperoxaluria. We outline our efforts to improve the diagnostic capacity of exome sequencing for these patients. We conducted a retrospective analysis of exome sequencing data from 77 pediatric urolithiasis patients with hyperoxaluria of unknown origin. Canonical exome sequencing analysis was performed to identify pathogenic variants in three known primary hyperoxaluria-related genes (AGXT , GRHPR , HOGA1) as per the guidelines of the American College of Medical Genetics. Then, extended exome sequencing analyses of 5′-untranslated region, non-canonical splicing site and copy number variant were performed on patients with negative diagnostic results in these three genes. Canonical exome sequencing analyses led to the diagnosis of primary hyperoxaluria in 20/77 (26%) patients, including eight, four, and eight patients diagnosed with type 1, 2 and 3 primary hyperoxaluria, respectively. Non-canonical splicing site analyses discovered a pathogenic variant in the HOGA1 gene, which led to the diagnosis of six additional patients with type 3 primary hyperoxaluria, while copy number variant analyses from exome sequencing data identified a 1.8 kb copy number loss that impacted the AGXT gene, resulting in the diagnosis of an additional type 1 primary hyperoxaluria case. Extended non-canonical splicing site and copy number variant analyses improve the diagnostic yield of canonical exome sequencing analysis for primary hyperoxaluria from 26% (20/77) to 35% (27/77) in 77 pediatric urolithiasis patients with hyperoxaluria. [ABSTRACT FROM AUTHOR]

Adults without diabetes are not completely healthy; they are probably heterogeneous with several potential health problems. The management of hemoglobin A1c (HbA1c) is crucial among patients with diabetes; but whether similar management strategy is needed for adults without diabetes is unclear. Thus, this study aimed to investigate the associations of visit-to-visit HbA1c variability with incident dementia and hippocampal volume among middle-aged and older adults without diabetes, providing potential insights into this question. We conducted a prospective analysis for incident dementia in 10,792 participants (mean age 58.9 years, 47.8 % men) from the UK Biobank. A subgroup of 3793 participants (mean age 57.8 years, 48.6 % men) was included in the analysis for hippocampal volume. We defined HbA1c variability as the difference in HbA1c divided by the mean HbA1c over the 2 sequential visits ([latter − former]/mean). Dementia was identified using hospital inpatient records with ICD-9 codes. T1-structural brain magnetic resonance imaging was conducted to derive hippocampal volume (normalized for head size). The nonlinear and linear associations were examined using restricted cubic spline (RCS) models, Cox regression models, and multiple linear regression models. During a mean follow-up (since the second round) of 8.4 years, 90 (0.8 %) participants developed dementia. The RCS models suggested no significant nonlinear associations of HbA1c variability with incident dementia and hippocampal volume, respectively (All P > 0.05). Above an optimal cutoff of HbA1c variability at 0.08, high HbA1c variability (increment in HbA1c) was associated with an increased risk of dementia (Hazard Ratio, 1.88; 95 % Confidence Interval, 1.13 to 3.14, P = 0.015), and lower hippocampal volume (coefficient, −96.84 mm3, P = 0.037), respectively, in models with adjustment of covariates including age, sex, etc. Similar results were found for a different cut-off of 0. A series of sensitivity analyses verified the robustness of the findings. Among middle-aged and older adults without diabetes, increasing visit-to-visit HbA1c variability was associated with an increased dementia risk and lower hippocampal volume. The findings highlight the importance of monitoring and controlling HbA1c fluctuation in apparently healthy adults without diabetes. • High visit-to-visit HbA1c variability was associated with an increased dementia risk. • High visit-to-visit HbA1c variability was associated with low hippocampal volume. • Monitoring HbA1c fluctuation is of great importance in adults without diabetes. [ABSTRACT FROM AUTHOR]

[Display omitted] • Double enzymatic hydrolysis combined with IL-US improved DPP-IV inhibitory activity. • IL-US pretreatment induced the exposure of active sites and hydrophobic amino acids. • IL-US pretreatment enhanced the gastrointestinal stability of collagen peptides. • Five new antidiabetic peptides were identified via LC-MS/MS and molecular docking. • The identified GPVGPPG sequence might be the most effective DPP-IV inhibitor. The present study focused on the effects of different preparation conditions on the activity and peptide sequence identification of DPP-IV inhibitory peptides based on discarded cowhide collagen and its regulation mechanism. The highest DPP-IV inhibitory activity (IC 50 of 3.04 ± 0.13 mg/mL) was obtained by the combination of double enzymatic hydrolysis (papain and compound protease) and optimized IL-US (ultrasound-assisted ionic liquids) pretreatment. The optimal pretreatment condition for ultrasonic power, ultrasonic time, ionic liquid/collagen ratio, and collagen concentration were 389 W, 25 min, 0.7, and 0.3 %, respectively. IL-US pretreatment induced the exposure of active sites and hydrophobic amino acids of collagen peptide, promoted the partial transformation of β-sheet to β-turn, and enhanced the stability of gastrointestinal digestion. Seven new peptides with DPP-IV inhibitory ability were identified. Importantly, further molecular docking showed that five peptides (GPVG, FGPGP, APGGAP, GPPGPT, and GPVGPPG) were proposed to be antidiabetic peptides from cowhide collagens. [ABSTRACT FROM AUTHOR]

Interactions between coal matrix and organics are vital for adsorption [1] , surfactant adsorption on lignite [2-5] , and solid–liquid separation of coal slurry [6-10]. Herein, for studying the interaction between hydrophobic surface (coal surface) and small molecules (organics), we measured interactions between organics [nitro and phenyl, pyridine, phenyl, alkyl chain (hexyl) or cycloalkyl (hexyl)] and the coal matrix (liptinite or inertinite) using atomic force microscopy. The strength of interaction followed the order: nitro and phenyl > pyridine > phenyl > alkyl chain (hexyl) > cycloalkyl (hexyl), and liptinite > inertinite. The liptinite has a greater hydrophobicity than inertinite, the greater the attraction force. The lignite surface and coal slurry were modified in an aqueous solution using five surfactants (dodecyl trimethyl ammonium chloride, DTAC; hexadecyl trimethyl ammonium chloride, CTAC; octadecyl trimethyl ammonium chloride, STAC; hexadecyl dimethyl benzyl ammonium chloride, HDBAC; and cetyl pyridine chloride, CPC). Subsequently, the moisture adsorption on treated lignite coal surface and the sedimentation of the treated tails was compared to characterize hydrophobic modifications which showed the order of CTAC > HDBAC > DTAC > CPC > STAC. Thus, the interaction between coal surface and organics needs to consider two rules: 1) The desolvation or the hydrophobic property of the coal surface; 2) The VDW force of the organics. The nature of the difference between rules No. 1 and 2 is that with the size of hydrophobic molecules increased to a surface, the H-bond structure between water molecules in the around would change a lot, which formats great hydrophobic property or desolvation. Interaction between organic reagents and coal reveals the mechanism of restraining moisture reabsorption of lignite and solid–liquid separation of coal slurry by organics. [ABSTRACT FROM AUTHOR]