Search

The potential utilization of intestine mucous and by‐products from the pig processing industry, was investigated. Pig intestine mucus contains intestinal alkaline phosphatase (IAP) which has been shown to function as the gut's mucosal defense factor. The extraction method was conducted at high‐pressure homogenization (HPH)‐assisted hierarchical extraction, and then IAP was purified by ion‐exchange chromatography. Also, the enzymatic properties and the dephosphorylation ability of IAP were investigated. After the extraction and purification process, IAP activity was 2980 U/ml, and purity was 95.71%. The optimal reaction conditions of IAP were at pH 9.6; the temperature was 37°C, Zn2+, Mg2+, and EDTA were a potent inhibitor of IAP activity. Furthermore, IAP showed dose‐dependent dephosphorylation to lipopolysaccharide (LPS), adenosine triphosphate (ATP), and CpG DNA in vitro. In summary, this study provides an efficient extraction and purification for IAP, which can be used as natural anti‐inflammatory material in the pharmaceutical and functional food industries. Practical applications: In this study, pig IAP with high purity and high activity was obtained by HPH‐ assisted fractional extraction of ammonium sulfate and acetone, followed by ion chromatography purification, which is simpler than the recombinant method of IAP, environmentally friendly, while reducing the process cost, and has high application value. Moreover, through the analysis of the IAP, it was found that IAP has strong dephosphorylating properties for inflammatory mediators such as LPS, ATP, and CpG DNA. Therefore, in practical application, IAP leaves a significant place for broad‐spectrum anti‐inflammatory function in human nutrition. [ABSTRACT FROM AUTHOR]