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Carbapenem resistance (CR) is an emerging health issue. Epidemiological surveys on carbapenem-resistant Gram-negative bacilli (CR-GNB) in Lebanon remain scarce. In this study, we determined the prevalence of CR-GNB isolated between 2015 to 2019 in three hospitals in northern Lebanon: 311 CR-Enterobacterales (out of 11210; 2.8%), 155 CR-Pseudomonas (out of 1034; 15%) and 106 CR- Acinetobacter (out of 184; 57.6%) were identified. CR mechanisms were determined for 146 randomly chosen isolates: the Carba NP test revealed an enzymatic resistance to carbapenems in 109 isolates (out of 146, 74.7%). Produced carbapenemases were evaluated by the NG-Test Carba5, NG-Test OXA-23 immunochromatographic assays and PCR. Carbapenemase-producing (CP) Enterobacterales expressed blaOXA-48-like, blaNDM-like and blaVIM-like genes and CP-Pseudomonas expressed blaIMP-like and blaVIM-like genes, whereas CP-Acinetobacter expressed blaOXA-23-like genes. The NG-Test Carba5 results were confirmed by PCR sequencing and revealed several variants, such as NDM-19, VIM-62 and OXA-162, never described so far in Lebanon. Isolates with discordant results were sequenced by WGS and highlighted novel variants of the natural oxacillinases of Pseudomonas aeruginosa: blaOXA-50-like genes. Their role in carbapenem resistance should be further studied. Overall, our findings highlight an alarming situation and encourage health care centers to establish performant registration systems that could help in limiting resistance spread. [ABSTRACT FROM AUTHOR]

Water quality models are useful tools to understand and mitigate eutrophication processes. However, gaining access to high-resolution data and fitting models to local conditions can interfere with their implementation. This paper analyzes whether it is possible to create a spatial model of nutrient water level at a local scale that is applicable in different geophysical and land-use conditions. The total nitrogen and phosphorus concentrations were modeled by integrating Geographical Information Systems, Remote Sensing, and Generalized Additive and Land-Use Changes Modeling. The research was based on two case studies, which included 204 drainage basins, with nutrient and limnological data collected during two seasons. The models performed well under local conditions, with small errors calculated from the independent samples. The recorded and predicted concentrations of nutrients indicated a significant risk of water eutrophication in both areas, showing the impact of agricultural intensification and population growth on water quality. The models are a contribution to the sustainable land-use planning process, which can help to prevent or promote land-use transformation and new practices in agricultural production and urban design. The ability to implement models using secondary information, which is easily collected at a low cost, is the most remarkable feature of this approach. [ABSTRACT FROM AUTHOR]

Roads are one of the main causes of loss of biodiversity, with roadkill one of the main causes of mortality. The aim of this research was to identify sites with a high probability of roadkill of medium and large mammals, and the environmental variables that would explain it. We used the favourability function (F) to build the predictive models. There were 57 explanatory variables, and we collected 685 records of 10 species of medium and large native wild mammals from the ECOBIO Uruguay databases. They were grouped into native forest and grassland species, according to the main habitat. Two models were developed, one with all the variables and one with the anthropogenic variables. For both groups, the model obtained with all the variables was the most significant according to the evaluation indices used. This made it possible to identify the hot spots of roadkill (F > 0.6) for each of the groups. The anthropic variables were the ones that best explained these hot spots. This allowed the identification of sites where the probability of roadkill is high and requires a monitoring plan to implement mitigation measures in the future. [ABSTRACT FROM AUTHOR]

Macrophages (Mφs) are instrumental regulators of the immune response whereby they acquire diverse functional phenotypes following their exposure to microenvironmental cues that govern their differentiation from monocytes and their activation. The complexity and diversity of the mycobacterial cell wall have empowered mycobacteria with potent immunomodulatory capacities. A heat-killed (HK) whole-cell preparation of Mycobacterium obuense (M. obuense) has shown promise as an adjunctive immunotherapeutic agent for the treatment of cancer. Moreover, HK M. obuense has been shown to trigger the differentiation of human monocytes into a monocyte-derived macrophage (MDM) type named Mob-MDM. However, the transcriptomic profile and functional properties of Mob-MDMs remain undefined during an activation state. Here, we characterized cytokine/chemokine release patterns and transcriptomic profiles of lipopolysaccharide (LPS)/interferon γ (IFNγ)-activated human MDMs that were differentiated with HK M. obuense (Mob-MDM(LPS/IFNγ)), macrophage colony-stimulating factor M-MDM(LPS/IFNγ)), or granulocyte/macrophage colony-stimulating factor (GM-MDM(LPS/IFNγ)). Mob-MDM(LPS/IFNγ) demonstrated a unique cytokine/chemokine release pattern (interleukin (IL)-10low, IL-12/23p40low, IL-23p19/p40low, chemokine (C-x-C) motif ligand (CXCL)9low) that was distinct from those of M-MDM(LPS/IFNγ) and GM-MDM(LPS/IFNγ). Furthermore, M-MDM(LPS/IFNγ) maintained IL-10 production at significantly higher levels compared to GM-MDM(LPS/IFNγ) and Mob-MDM(LPS/IFNγ) despite being activated with M1-Mφ-activating stimuli. Comparative RNA sequencing analysis pointed to a distinct transcriptome profile for Mob-MDM(LPS/IFNγ) relative to both M-MDM(LPS/IFNγ) and GM-MDM(LPS/IFNγ) that comprised 417 transcripts. Functional gene-set enrichment analysis revealed significant overrepresentation of signaling pathways and biological processes that were uniquely related to Mob-MDM(LPS/IFNγ). Our findings lay a foundation for the potential integration of HK M. obuense in specific cell-based immunotherapeutic modalities such as adoptive transfer of Mφs (Mob-MDM(LPS/IFNγ)) for cancer treatment. [ABSTRACT FROM AUTHOR]