1. Graphene Oxide Loaded on TiO 2 -Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts.
- Author
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Cao, Xu, Wu, Keyi, Wang, Caiyun, Guo, Yatong, Lu, Ran, Wang, Xin, and Chen, Su
- Subjects
REVERSE transcriptase polymerase chain reaction ,GRAPHENE oxide ,TITANIUM dioxide ,X-ray photoelectron spectroscopy ,GINGIVA ,FIBROBLASTS ,INTEGRINS ,CELL adhesion - Abstract
Surface topography, protein adsorption, and the loading of coating materials can affect soft tissue sealing. Graphene oxide (GO) is a promising candidate for improving material surface functionalization to facilitate soft tissue integration between cells and biomaterials. In this study, TiO
2 nanotubes (TNTs) were prepared by the anodization of Ti, and TNT-graphene oxide composites (TNT-GO) were prepared by subsequent electroplating. The aim of this study was to investigate the effect of TNTs and TNT-GO surface modifications on the behavior of human gingival fibroblasts (HGFs). Commercially pure Ti and TNTs were used as the control group, and the TNT-GO surface was used as the experimental group. Scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction were used to perform sample characterization. Cell adhesion, cell proliferation, cell immunofluorescence staining, a wound-healing assay, real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and Western blotting showed that the proliferation, adhesion, migration, and adhesion-related relative gene expression of HGFs on TNT-GO were significantly enhanced compared to the control groups, which may be mediated by the activation of integrin β1 and the MAPK-Erk1/2 pathway. Our findings suggest that the biological reactivity of HGFs can be enhanced by the TNT-GO surface, thereby improving the soft tissue sealing ability. [ABSTRACT FROM AUTHOR]- Published
- 2022
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