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With the development of big data and cloud computing, more and more data owners store their data with encrypted form in the cloud server. To retrieve some data they are interested in, an important technology called searchable encryption can be adopted. Sometimes, the encrypted data are desired to be shared to some users by the data owners without destroying the user privacy. To address this problem, many works of delegated searchable encryption (DSE) have been studied. However, in the existing DSE schemes, the delegated users can make keyword search over the encrypted data without any restrictions. In some systems such as E-health record system in which the data are sensitive, it is desired that the delegated users can only make search with some specified keywords designated by the data owners. In this work, we present an efficient solution to this problem and give a concrete construction. Then we show that our construction is efficient and practical for real applications by experimental results. [ABSTRACT FROM AUTHOR]

Microorganisms in the soil that have been contaminated with heavy metals for a long-time exhibited resistance to heavy metals. In recent years, the use of resistant microbes and heavy metal-tolerant plants to repair heavy metal-contaminated soil has become an increasingly hot subject. In this study, heavy metal-tolerant bacteria ZG7 was isolated from the mining soils and injected into Bidens pilosa L. and Medicago sativa L.. The results revealed that: (1) Compared to the non-inoculated group, inoculation with ZG7 notably increased the dry weight and chlorophyll content in plants under heavy metal stress, as well as promoted the content of several types of antioxidant chemicals; (2) Combined remediation improved soil enzyme activities, with soil catalase, sucrase, and phosphatase activities remarkably increased by 136.06%, 124.17% and 180.14% in the ZG7- Bidens pilosa L.; And soil catalase, urease, and sucrase activities promoted by 68.07%, 130.08% and 239.79% in the ZG7- Medicago sativa L.; (3) The content of Pb and Zn in the ZG7- Medicago sativa L. combination soil were considerably lower (9.28% and 6.2%, respectively) than no strain group, but there was no substantial change in soil heavy metal content when combined with the Bidens pilosa L. remediation when compared to no strain group. In summary, the combination of strain ZG7 and plants had a good effect on the remediation of heavy metal contaminated soils, and ZG7- Medicago sativa L. had the best effect, which is a reliable technique for effective control of heavy metal pollution. This study provided theoretical support for microbial-phytoremediation joint remediation. [ABSTRACT FROM AUTHOR]

Saliva has emerged as a promising noninvasive biofluid for the diagnosis of oral and systemic diseases, including viral infections. During the coronavirus disease 2019 (COVID-19) pandemic, a growing number of studies focused on saliva-based detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Taking advantage of the WoS core collection (WoSCC) and CiteSpace, we retrieved 1021 articles related to saliva-based detection of SARS-CoV-2 and conducted a comprehensive bibliometric analysis. We analyzed countries, institutions, authors, cited authors, and cited journals to summarize their contribution and influence and analyzed keywords to explore research hotspots and trends. From 2020 to 2021, research focused on viral transmission via saliva and verification of saliva as a reliable specimen, whereas from 2021 to the present, the focus of research has switched to saliva-based biosensors for SARS-CoV-2 detection. By far, saliva has been verified as a reliable specimen for SARS-CoV-2 detection, although a standardized procedure for saliva sampling and processing is needed. Studies on saliva-based detection of SARS-CoV-2 will promote the development of saliva-based diagnostics and biosensors for viral detection. Collectively, our findings could provide valuable information to help scientists perceive the basic knowledge landscapes on saliva-based detection of SARS-CoV-2, the past and current research hotspots, and future opportunities. [ABSTRACT FROM AUTHOR]

Gastric cancer is a common malignant tumor, and due to its insidious onset and limited screening methods, most patients are diagnosed with advanced disease and have a poor prognosis. The circRNA in exosomes has an essential role in cancer diagnosis and treatment. However, the part of hsa_circ_0014606 within exosomes in gastric cancer progression is unclear. Firstly, we extracted exosomes from the serum of gastric cancer patients and healthy individuals by ultracentrifugation and analyzed the expression of hsa_circ_0014606 in both exosomes; then knocked down hsa_circ_0014606 in vivo and in vitro, respectively, to observe its effect on the physiological function of gastric cancer cells; finally, we used bioinformatics to screen hsa_circ_0014606 targeting miRNAs and mRNAs, and experiments were performed to verify the interrelationship between the three. The results showed that the level of hsa_circ_0014606 in the serum exosomes of gastric cancer patients was significantly higher than that of the healthy population. The knockdown of hsa_circ_0014606 slowed the proliferation of gastric cancer cells, significantly reduced migration and invasion ability, accelerated apoptosis, and reduced tumor size in mice. In addition, the expression of hsa_circ_0014606 was negatively correlated with the expression of miR-514b-3p and positively correlated with the expression of heterogeneous nuclear ribonucleoprotein C (HNRNPC). In conclusion, hsa_circ_0014606 exerted a pro-cancer effect indirectly through miR-514b-3p targeting gene HNRNPC, and this study provides a new potential target for treating gastric cancer. [ABSTRACT FROM AUTHOR]

The conversion of biomass energy sources is a crucial direction in the transformation of energy resources, such as cellulose to high-value chemicals. As an important platform compound, 5-hydroxymethylfurfural (HMF) is one of the worthwhile products from biomass. Previously, the use of Hf/ZSM-5 catalyst demonstrated excellent catalytic performance, with the yield of HMF reaching 67.50% under optimal reaction conditions (120 min, 190 °C, H2O(NaCl)/THF). Based on the previous work, the effect of a series of metal salts on the preparation of HMF from cellulose catalyzed by Hf/ZSM-5 catalyst was investigated in this paper. This result demonstrates the addition of AlCl3 promoted the production of HMF yield and AlCl3 completed with excellent performance among five metal salts. Moreover, the yield of HMF was improved to 70.95% by adjusting the optimal reaction conditions. [ABSTRACT FROM AUTHOR]

Chronic diabetic wounds are at lifelong risk of developing diabetic foot ulcers owing to severe hypoxia, excessive reactive oxygen species (ROS), a complex inflammatory microenvironment, and the potential for bacterial infection. Here we develop a programmed treatment strategy employing live Haematococcus (HEA). By modulating light intensity, HEA can be programmed to perform a variety of functions, such as antibacterial activity, oxygen supply, ROS scavenging, and immune regulation, suggesting its potential for use in programmed therapy. Under high light intensity (658 nm, 0.5 W/cm2), green HEA (GHEA) with efficient photothermal conversion mediate wound surface disinfection. By decreasing the light intensity (658 nm, 0.1 W/cm2), the photosynthetic system of GHEA can continuously produce oxygen, effectively resolving the problems of hypoxia and promoting vascular regeneration. Continuous light irradiation induces astaxanthin (AST) accumulation in HEA cells, resulting in a gradual transformation from a green to red hue (RHEA). RHEA effectively scavenges excess ROS, enhances the expression of intracellular antioxidant enzymes, and directs polarization to M2 macrophages by secreting AST vesicles via exosomes. The living HEA hydrogel can sterilize and enhance cell proliferation and migration and promote neoangiogenesis, which could improve infected diabetic wound healing in female mice. The treatment of infected diabetic wounds faces obstacles of bacterial infection, hypoxia, hyperexpression of reactive oxygen species, and inflammation. Here, the authors address these issues by developing a programmed treatment strategy that utilizes live Haematococcus to promote healing of diabetic wounds in a comprehensive manner. [ABSTRACT FROM AUTHOR]

In this paper, we study a class of nonconvex and nonsmooth optimization problems, whose objective function can be split into two separable terms and one coupling term. Alternating proximal gradient methods combining with extrapolation are proposed to solve such problems. Under some assumptions, we prove that every cluster point of the sequence generated by our algorithms is a critical point. Furthermore, if the objective function satisfies Kurdyka–Łojasiewicz property, the generated sequence is globally convergent to a critical point. In order to make the algorithm more effective and flexible, we also use some strategies to update the extrapolation parameter and solve the problems with unknown Lipschitz constant. Numerical experiments demonstrate the effectiveness of our algorithms. [ABSTRACT FROM AUTHOR]

Introduction and hypothesis: Given the younger age of cervical cancer patients and improved postoperative survival, postoperative quality of life should be a reason for concern, particularly given the prevalence of pelvic floor dysfunction. High uterosacral ligament suspension (HUS) has been deemed the more consistently successful surgery for the treatment of mid-pelvic abnormalities. HUS intraoperatively prevents pelvic floor dysfunction effectively. Methods: We demonstrate the steps of surgery using surgical video and photographs. The uterosacral ligament is fan shaped and connected to the fascial and extraosseous membranes on the surface of the anterior sacral foramen of the 2nd, 3rd, and 4th sacral vertebrae. Given that the uterosacral ligament was fan-shaped, the fan-shaped suture with three stitches was more compatible with the original anatomy. Results: Thirty patients with HUS who underwent thorough hysterectomy had no complications, operation time 230.82 ± 43.61 min, and blood loss 62.32 ± 37.25 ml. The urinary catheter was successfully removed 1 week after the operation, and no pelvic organ prolapse, including vaginal anterior and posterior wall prolapse, or rectocele, occurred after 3 years of follow-up. Conclusion: The uterosacral ligament fulfills the role of supporting, pulling, and suspending the uterus. We should exploit the advantage of fully exposing the uterosacral ligament in radical hysterectomy. Performing HUS to prevent pelvic organ prolapse following radical hysterectomy is a procedure worthy of investigation and promotion. [ABSTRACT FROM AUTHOR]

We previously identified a unique nucleus, the cerebrospinal fluid (CSF)-contacting nucleus. This study aims to understand its gene architecture and preliminarily suggest its functions. The results showed that there were about 19,666 genes in this nucleus, of which 913 were distinct from the dorsal raphe nucleus (non-CSF contacting). The top 40 highly-expressed genes are mainly related to energy metabolism, protein synthesis, transport, secretion, and hydrolysis. The main neurotransmitter is 5-HT. The receptors of 5-HT and GABA are abundant. The channels for Cl–, Na+, K+, and Ca2+ are routinely expressed. The signaling molecules associated with the CaMK, JAK, and MAPK pathways were identified accurately. In particular, the channels of transient receptor potential associated with nociceptors and the solute carrier superfamily members associated with cell membrane transport were significantly expressed. The relationship between the main genes of the nucleus and life activities is preliminarily verified. [ABSTRACT FROM AUTHOR]

Psychological dependence is the most important factor leading to relapse, few studies have examined whether in-group stereotypes exist in drug abusers, while it's helpful for drug abusers in getting rid of psychological dependence. To investigate the presence of in-group stereotypes and the neural mechanisms in drug abusers, two experiments were designed in this study. Experiment 1 used a classification–verification paradigm and Experiment 2 used The Extrinsic Affect Simon Task (EAST), simultaneous collection of EEG data from China. 18 and 17 males were analyzed respectively in Experiment 1 and 2. The results in Experiment 1 showed that (1) there was no significant difference in reaction times or N400 amplitude between the condition "Drug abusers-Negative words" and the condition "Drug abusers-Positive words". In Experiment 2, we found that (2) participants in the condition "Drug abusers-Negative words" scored higher in accuracy rates than in the condition "Drug abusers-Positive words". (3) Participants in the condition "Drug abusers-Negative words" were shorter than "Drug abusers-Positive words" in reaction times (RTs). (4) Participants in the condition "Drug abusers-Negative words" were lower than "Drug abusers-Positive words" in the peak of N400. The conclusion is that there are significant negative implicit in-group stereotypes among Chinese male drug abusers. The experimental results and the uniqueness of Chinese male drug abusers in this study were discussed. [ABSTRACT FROM AUTHOR]

Correction to: IL-17A promotes fatty acid uptake through the IL-17A/IL-17RA/ p-STAT3/FABP4 axis to fuel ovarian cancer growth in an adipocyte-rich microenvironment 3rhIL-17A increased the uptake of PA in OvCa cells via the IL-17A/IL-17RA/p-STAT3/FABP4 axis. ** p < 0.01. g The sections were prepared from the tumor tissues of WT and IL-17A -/- mice, and IHC staining was performed to determine FABP4 expression was performed. [Extracted from the article]

Aiming at recovery of uranium (U) from low-grade uranium ore with a high fluorine content, column bioleaching of uranium extraction with an indigenous mesophilic consortium was studied. The performance of the bacterial community and the effect of chemical parameters on column bioleaching of uranium were investigated. The results showed that U extraction reached a high value of 95.69%, including acid preleaching (65.53%) and bioleaching (30.16%), in a 124-day period of the leaching process. The composition and diversity of the bacterial community were found to be significantly changing in the acid preleaching and bioleaching stages; nevertheless, they existed in residue and pregnant leaching solution samples for the same stage and showed little difference. Additionally, dominant genera in the solid samples, including Stenotrophomonas, Pseudomonas, Brevundimonas Clostridium_sensu_stricto, Bacillus, Clostridium XI, and Acidithiobacillus, and the Acidithiobacillus genus were ubiquitous throughout the leaching column, which was in favour of U extraction. [ABSTRACT FROM AUTHOR]

Atmospheric corrosion is ubiquitous in China but varies a lot among different regions covering the cold, temperate, and tropical zones. Categorizing the atmospheric corrosivity and plotting precise atmospheric corrosion map remain key interest for a variety of industries. The present work proposed an atmospheric corrosion map of China for hot-dip galvanized steels, which was constructed by inverse distance weighting (IDW) interpolation algorithm based on both the measured corrosion rates of coupons exposed at 2393 inland test stations and calculated corrosion rates from a prevalent dose-response function in 2918 sites in coastal regions. When the corrosion category was used as the criterion, the IDW interpolation algorithm of power 2 performed best. Cross-validation results confirmed that the prediction accuracy of IDW interpolation reached 85.6%. Based on the corrosion map, the categories of atmospheric corrosivity in China could be determined. [ABSTRACT FROM AUTHOR]

For most projection methods, the operator of a variational inequality problem is assumed to be monotone (or pseudomonotone) and Lipschitz continuous. In this paper, we present a projection-like method to solve quasimonotone variational inequality problems without Lipschitz continuity. Under some mild assumptions, we prove that the sequence generated by the proposed algorithm converges to a solution. Numerical experiments are provided to show the effectiveness of the method. [ABSTRACT FROM AUTHOR]

Methicillin-resistant Staphylococcus aureus (MRSA) has become a rising clinical problem as its occurrence has increased due to the overuse and misuse of antibiotics. In this work, upconversion nanoparticles@AgBiS2 core-shell were produced with enhanced photothermal transformation efficiency and ability to produce reactive oxygen species for synergistic photodynamic photothermal and photodynamic antibacterial performance. The nanoparticles exhibit good antibacterial effects in vitro and satisfactory therapeutic performance on healing MRSA-infected wounds in vivo experiments. RNA-sequencing technique has been used to investigate and reveal that photothermal-photodynamic therapy using the nanoparticles can interfere with metabolic processes such as galactose metabolism in MRSA bacteria, destroy the transport system on the surface of MRSA, and affect quorum sensing to hinder the formation of biofilms to achieve effective antibacterial efficacy. It was demonstrated that this work presents an alternative near-infrared photoactive multimodal nanostructure for antibacterial applications. [ABSTRACT FROM AUTHOR]

In cutaneous cosmetology surgery, local injection or coated anesthetics are generally used to provide analgesia at the treatment site to achieve painless operation. Due to the barrier of corneum, topical cream may cause uncertain dosage and delayed analgesia. Local injection has problems such as pain, infection, and misoperation. Therefore, it is necessary to develop a painless and rapid administration method for local anesthesia. Here, a lidocaine/hyaluronic acid bubble microneedle patch (Lido/HA bMNP) was prepared for rapid drug delivery and efficient analgesia. The bubble structure between microneedles (MNs) and the backing layer allowed the MNs to efficiently penetrate into the skin and remove from the backing layer under shear force to rapidly complete the administration. Drugs were quickly released with the dissolution of HA within 15 s, which immediately played an analgesic effect and lasted for 1 h. Lido/HA bMNP could deliver precise doses to the skin in an extremely short time, which had the advantages of convenient operation, high biosafety, rapid onset of analgesia, and reasonable pain relief time. This patch provided an alternative way for local anesthesia and it was a promising transdermal drug delivery method for the realization of high quality and efficiency "painless medical beauty". [ABSTRACT FROM AUTHOR]

α-Synuclein (α-syn) is the main protein component of Lewy bodies, the major pathological hallmarks of Parkinson's disease (PD). C-terminally truncated α-syn is found in the brain of PD patients, reduces cell viability and tends to form fibrils. Nevertheless, little is known about the mechanisms underlying the role of C-terminal truncation on the cytotoxicity and aggregation of α-syn. Here, we use nuclear magnetic resonance spectroscopy to show that the truncation alters α-syn conformation, resulting in an attractive interaction of the N-terminus with membranes and molecular chaperone, protein disulfide isomerase (PDI). The truncated protein is more toxic to mitochondria than full-length protein and diminishes the effect of PDI on α-syn fibrillation. Our findings reveal a modulatory role for the C-terminus in the cytotoxicity and aggregation of α-syn by interfering with the N-terminus binding to membranes and chaperone, and provide a molecular basis for the pathological role of C-terminal truncation in PD pathogenesis. C-terminal truncation of a-syn results in a more extended and exposed conformation, providing further insight into the pathological role of this truncation event in the progression of Parkinson's disease. [ABSTRACT FROM AUTHOR]

A novel self-template sacrifice strategy was reported to prepare highly ordered mesoporous carbon (OMC) nanoparticles with idiographic hollow and unique groove structure. Besides, we successfully accomplished multi-morphology interconversion by simply regulating initial reaction temperature and the concentration of hydrochloric acid, and the particle sizes could be easily tuned from 700 to 190 nm. Meaningfully, a "pyrolysis-sagging" mechanism was further proposed for the comprehension of the groove structure, which provided a brand-new and oversimplified soft template principal for the synthesis of OMC with novel morphology. Moreover, the self-template sacrifice strategy was rationalized through a simplified replenishment of the nucleation and growth stages as an extension. The as synthesized materials were used as carriers for furosemide to evaluate the drug adsorption and release performance, endowing them with significant prospect in the field of biomedicine. [ABSTRACT FROM AUTHOR]

Background: Chronic kidney disease (CKD) has become a worldwide health problem among aging populations. However, epidemiological information on Chinese elderly people with CKD is still lacking. This study aimed to investigate the epidemiological features and associated risk factors of CKD in aging population in China. Methods: In this cross-sectional study, a total of 37,533 individuals aged ≥ 65 years were enrolled in Binhai from January to December 2018. The crude and standardized prevalence of CKD were calculated. Associations of metabolism-related indicators with CKD were examined using univariate and multivariate analyses. Results: The overall prevalence of CKD was 17.7% (95% confidence interval 17.3–18.1%) in this Chinese elderly population. The prevalence was 17.5% among men (95% CI 17.0–18.1%) and 17.8% among women (95% CI 17.3–18.4%). The mean eGFR was 84.22 (SD ± 12.87) mL/min/1.73 m2, with the median value higher for women than for men. Conclusion: Our study shows a high prevalence of CKD among Chinese elderly population. Aging, pre-HTN, HTN, elevated triglyceride, and FBG were associated with the risk of CKD. More attention should be paid to metabolic diseases to prevent CKD in the elderly. [ABSTRACT FROM AUTHOR]

Due to high content of carbonate and sulfide in ore, precipitation of calcium compounds is blocking the in-situ leaching system at Mengqiguer sandstone type deposit. Autoclave CO2 + O2 leaching tests were conducted in respecting the blockage and uranium migration. Result shows that: Most uranium was recovered within the first hour. Calcite and dolomite precipitated in the initial stage of leaching because of high pH value. As pH decreases rapidly after CO2 injection, calcite and dolomite did not precipitate. Gypsum is not saturated during the whole test. HCO3-, pH and Eh, controlled by CO2 + O2 injection are key factors influencing uranium leaching. [ABSTRACT FROM AUTHOR]

Integration of human papillomavirus (HPV) DNA into the human genome is considered as a key event in cervical carcinogenesis. Here, we perform comprehensive characterization of large-range virus-human integration events in 16 HPV16-positive cervical tumors using the Nanopore long-read sequencing technology. Four distinct integration types characterized by the integrated HPV DNA segments are identified with Type B being particularly notable as lacking E6/E7 genes. We further demonstrate that multiple clonal integration events are involved in the use of shared breakpoints, the induction of inter-chromosomal translocations and the formation of extrachromosomal circular virus-human hybrid structures. Combined with the corresponding RNA-seq data, we highlight LINC00290, LINC02500 and LENG9 as potential driver genes in cervical cancer. Finally, we reveal the spatial relationship of HPV integration and its various structural variations as well as their functional consequences in cervical cancer. These findings provide insight into HPV integration and its oncogenic progression in cervical cancer. The molecular mechanisms underlying cervical carcinogenesis following integration of HPV DNA into the human genome remain elusive. Here, the authors perform long-read sequencing in 16 HPV16-positive cervical tumors and identify distinct integration types, structural variations and potential driver genes. [ABSTRACT FROM AUTHOR]

Due to the small size of uranium minerals, the occupations of U4+ and U6+, Th and Pb in uraninite is little studied. The coarse giant uraninite (CGU) from Miyi with size up to 1 cm is one of the best samples for studying uraninite mineralogical property. CGU mainly composed of UO2, ThO2, PbO, CaO. There are three forms of occurrence of U, namely UO2, UO3, and UO2+x; and impurity such as ThO2, PbO, CaO may entering the mineral lattice, which can affect mineral properties and facilitate the chemical reactions during mineralization and leaching processes. [ABSTRACT FROM AUTHOR]