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Pulmonary invasive fungal infection in immunocompromised hosts is difficult to diagnose, and current tools for diagnosis or monitoring of response to antifungal treatments have inherent limitations. Droplet digital PCR (ddPCR) has emerged as a promising tool for pulmonary pathogen detection with high sensitivity. This study presents a novel ddPCR panel for rapid and sensitive identification of pulmonary fungal pathogens. First, a ddPCR method for detecting three fungal genera, including Pneumocystis, Aspergillus, and Cryptococcus, was established and evaluated. Then, the clinical validation performance of ddPCR was compared with that of qPCR using 170 specimens, and the 6 specimens with inconsistent results were further verified by metagenomics next-generation sequencing, which yielded results consistent with the ddPCR findings. Finally, the area under the ROC curve (AUC) was used to evaluate the efficiency of ddPCR. While the qPCR identified 16 (9.41%) cases of Aspergillus and 6 (3.53%) cases of Pneumocystis, ddPCR detected 20 (11.76%) Aspergillus cases and 8 (4.71%) Pneumocystis cases. The AUC for Aspergillus, Cryptococcus, and Pneumocystis was 0.974, 0.998, and 0.975, respectively. These findings demonstrated that the ddPCR assay is a highly sensitive method for identifying pathogens responsible for invasive fungal pulmonary infections, and is a promising tool for early diagnosis. [ABSTRACT FROM AUTHOR]

Over the years, it has become obvious that simultaneous backfill grouting is of great significance to the design of the tunnel lining and process control of shield construction. It is essential to know the grout pressure distribution around the tunnel lining. The purpose of this study is to seek an alternative method to simulate the grout diffusion process and obtain pressure distribution of different time during simultaneous backfill grouting of tunnel construction. A lattice Boltzmann method (LBM) based free surface flow model is introduced and modified, and a Smagorinsky large-eddy simulation model serves to deal with the small-scale turbulent problem, which is a challenge for LBM to simulate the large-scale real-world engineering. The simulation results obtain the grout filling state with time and pressure distribution around the tunnel lining. Additionally, the grout pressures on site are measured at a ring in line 8 of Chengdu Rail tunnel, and the measurement results are also compared with ones taken from numerical simulation, it is showed that the numerical results are consistent with that of measurement on the whole. Through simulating different grouting strategies, it is concluded that for four-holes grouting schemes, the grouting pressures of upper holes are significant for the grout pressure distribution and should be paid most attention in construction. [ABSTRACT FROM AUTHOR]

Childhood trauma and empathy deficits have been found to be related to borderline personality disorder (BPD), but the mechanisms of action remain unclear. This study aims to investigate the characteristics of empathy in male offenders with BPD tendencies and to test its mediating role in the relationship between childhood trauma and BPD tendencies. Two hundred and fifty-seven male offenders and 43 male nonoffender controls completed the Childhood Trauma Questionnaire Short From (CTQ-SF), the Questionnaire of Cognitive and Affective Empathy (QCAE) and the Chinese version of the computerized Yoni Task. ANOVA, Mixed-model ANOVAs and the PROCESS macro were used to analyze the inventory data and task performances. Compared to male offenders without BPD tendencies and nonoffender controls, offenders with BPD tendencies showed more severe childhood abuse, lower cognitive empathy and poorer task performances in second-order cognitive and second-order affective theory of mind. Furthermore, cognitive empathy was found to play a mediating role in the relationship between childhood trauma and BPD tendencies. Our findings indicate the effects of childhood trauma and empathy impairments on BPD and suggest the possible application value of the precision empathy ability training in the intervention of offenders with BPD. [ABSTRACT FROM AUTHOR]

Five species of Unionicola Haldeman, 1842 (Acari: Hydrachnidia: Unionicolidae) were collected between 2018 and 2019. Notably, Unionicola (Armatax) armata Walter, 1929, previously unrecorded in China, is now documented for the first time in the country. Additionally, Unionicola newsumi Wiles, 1999 has been reclassified and assigned to the new subgenus Vespicola within the genus Unionicola Haldeman, 1842. These findings also led to the establishment of three new subgenera: Micronodusgen. nov., Vespicolagen. nov., and Lingshuiataxgen. nov. including four species: Unionicola soluplatussp. nov. U. jinisp. nov. U. caudipessp. nov. and U. armatallasp. nov. This marks the first description and documentation of these subgenera and species. [ABSTRACT FROM AUTHOR]

A brief introduction of Chinese Hygrobatidae taxonomy is reviewed. A new species, Sinibates xinyaoae Li, Smit & Guo sp. nov. from Hainan province is described representing a new genus Sinibates Li, Smit & Guo gen. nov. A second species, Hygrobates (Minibates) cf. dobriyali Pešić & Smit, 2019, from Hunan province represents a new subgenus, Minibates Li, Smit & Guo subgen. nov. in Hygrobates. The other new species, Mixobates neoamurensis Li & Guo sp. nov. from Inner Mongolia Autonomous Region adds a newly recorded genus, Mixobates Thor, 1905 from China.Text: LSIDhttps://www.zoobank.org/urn:lsid:zoobank.org:pub:3EF1A166-30A4-4761-A133-E00F40B68007Genus: Sinibates Li, Smit & Guo gen. nov.: LSID https://www.zoobank.org/urn:lsid:zoobank.org:act:D4F94642-D47C-4200-B36B-C695A54166B4Species: Sinibates xinyaoae Li, Smit & Guo sp. nov.: LSID https://www.zoobank.org/urn:lsid:zoobank.org:act:23C6986A-128D-4906-9027-926891B2C138Subgenus: Minibates Li, Smit & Guo subgen. nov.: LSID https://www.zoobank.org/urn:lsid:zoobank.org:act:142B5C42-140C-4E6A-B38E-180F8C442B1BSpecies: Mixobates neoamurensis Li & Guo sp. nov.: LSID https://www.zoobank.org/urn:lsid:zoobank.org:act:9B494E8C-81F7-4859-8DC3-88392DCDB67A [ABSTRACT FROM AUTHOR]

Maternal high-fat diet (HFD) is a detrimental factor in developing glucose intolerance, obesity, and islet dysfunction. However, the effect of artemisinin on maternal HFD and whether it is related to the alterations of islet function is seldom studied since artemisinin treatments not only attenuate insulin resistance (IR) and restore islet ß cell function in Diabetes mellitus type 2. Female rats were randomly fed a HFD (45% kcal from fat), HFD + artemisinin, or a regular chow diet (RCD) before pregnancy and during gestation. Glucose metabolism and the β cell phenotypes were assessed. Maternal HFD increased islet load in female rats, proliferation of pancreatic β cells, increased insulinogen, and decreased insulin secretion response to high glucose stimulation with delayed insulin release, increased fasting glucose, and glucose area under the curve compared with the general diet group. HFD inhibited expression of Foxo1 and PAX6 in female rats. Under the effect of both HFD and pregnancy, islet load was further increased, insulinogen was further increased, and fasting insulin level and fasting glucose were higher than RCD fed general-pregnancy group. ALDH1a3 transdifferentiation and PAX6, Foxo1, and PDX1 expression were increased in islets of high-fat pregnant rats. When adding artemisinin in HFD treated pregnant rats, islet function was significantly improved. Intervention with artemisinin in maternal HFD resulted in reduced islet size, decreased number of β-cells and improved islet microcirculation, insulin processing shear process, decreased insulinogen/insulin ratio, and restored islet function through increased expression of PC1/3. [ABSTRACT FROM AUTHOR]

A new species, Momonides neolandbergi Li & Guo sp. nov. is described in detail, which is the fifth species all over the world in Momonidinae Lundblad, 1941, Momoniidae K. Viets, 1926. The family and subfamily are newly recorded for Chinese fauna. The new species are recorded by camera, optical microscope, and scanning electron microscope. Publication:New species: [ABSTRACT FROM AUTHOR]

This research is focussed to quantify IGF1 by electroanalytical analysis on InterDigitated electrode surface and characterized by the microscopic observations. For the detection, antibody and aptamer were used to analyze the level of IGF1. The sandwich pattern (aptamer-IGF1-antibody) was designed on the chemically modified IDE surface and reached the limit of detection to 10 fM with 100 folds enhancement in the sensitivity. Different control experiments (absence of IGF1, binding with IGF2 and with non-complementary aptamer) were failed to show the current changes, discriminated the specific detection. A good detection strategy is to complement the currently following imaging systems for AAA. [ABSTRACT FROM AUTHOR]

Semen cuscutae is commonly used to treat male infertility (MI), and semen cuscutae flavonoid (SCF) is the main active component of semen cuscutae. The therapeutic mechanism of SCF on MI is still unclear. To clarify the mechanisms of SCF against MI. Network pharmacology and molecular docking were used to predict the potential pathways of SCF against MI. Primary Sertoli cells (SCs) were extracted from testis of 60-day-old rats and divided into Control, Model, and 3 treatment groups. The Control and Model groups were given normal medium, the treatment groups were treated with various concentrations of SCF-containing medium (200, 400, and 800 μg/mL). After 24 h, the Model and treatment groups were exposed to heat stress at 43 °C for 15 min. Western blotting and immunohistochemistry were used to detect the expression of targets. Network pharmacology indicated that the treatment of SCF on MI was closely related to PI3K-AKT signaling pathway. The in vitro experiments showed that SCF could up-regulated the expression of AKT, AR, occludin, and Ki67, and down-regulated the expression of CK-18 in SCs after heat stress. The AKT inhibitor could block this process. SCF can treat MI by regulating the proliferation and differentiation of SCs and the integrity of the blood-testis barrier. The study could provide experimental basis for clinical research. [ABSTRACT FROM AUTHOR]

The present paper deals with two new species, Atractides (Atractides) bitergumus Zhang & Guo sp. nov. and Atractides (Tympanomegapus) tergumus Zhang & Guo sp. nov., and a new record of a species, Atractides (Atractides) incertus, from Hainan Province, P. R. China. These species are described and illustrated in detail. Article: Atractides (Atractides) bitergumus: Atractides (Tympanomegapus) tergumus: [ABSTRACT FROM AUTHOR]

The genus Dyneogamasus is transferred to the subfamily Pergamasinae, and the generic diagnosis is revised. A new mite species, Dyneogamasus serratus Yao & Jin sp. nov., is described and illustrated based on females from Fujian province, China. A key to the known species of the genus is given. [ABSTRACT FROM AUTHOR]